FastQCFastQC Report
Sat 14 Jan 2017
SRR2933728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041393
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1209415.924434932421145No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC57390.28113156065490574No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC33180.1625360721820835No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA22710.11124756477562137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG453.8562575E-1045.01
CGTTTTT584200.044.4376951
CTTCGCG1150.041.086961
GTTTACG556.184564E-1140.9090921
GTTTTTT650500.040.3651052
CTGCGCG1500.039.0000041
GCGCGAC356.2506733E-638.5714269
CGCGACC356.2506733E-638.57142610
GACCGAT7300.037.9109579
CCGTTCG301.14023234E-437.4999961
TGGGCGA26350.037.4857676
TTGGGAC47050.036.631245
CGGGTAT1600.036.56256
TCTTGCG4250.036.529411
TACGGGA2350.036.382984
GGACCGA8300.036.32538
GGCCGAT5850.036.1538438
TTGGGAT86450.036.1509555
GGCGATC8800.036.0511368
CTCGTTG1500.036.0000041
Produced by FastQC (version 0.11.5)