##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2041393
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.482950122783805	34.0	31.0	34.0	31.0	34.0
2	32.69125788126049	34.0	31.0	34.0	31.0	34.0
3	32.723594623867136	34.0	31.0	34.0	31.0	34.0
4	36.22159868286018	37.0	35.0	37.0	35.0	37.0
5	36.23418322684559	37.0	35.0	37.0	35.0	37.0
6	36.217664604512706	37.0	37.0	37.0	35.0	37.0
7	36.291398569506214	37.0	37.0	37.0	35.0	37.0
8	36.2019738482497	37.0	37.0	37.0	35.0	37.0
9	38.028677966467015	39.0	39.0	39.0	35.0	39.0
10	37.52782389280261	39.0	37.0	39.0	35.0	39.0
11	37.38839214203242	39.0	37.0	39.0	34.0	39.0
12	37.48519515840409	39.0	37.0	39.0	35.0	39.0
13	37.515518569917695	39.0	37.0	39.0	35.0	39.0
14	38.84823892312749	40.0	38.0	41.0	35.0	41.0
15	38.881752313248846	40.0	38.0	41.0	35.0	41.0
16	38.89793880943062	40.0	38.0	41.0	35.0	41.0
17	38.84100905607102	40.0	38.0	41.0	35.0	41.0
18	38.336086681986274	39.0	38.0	40.0	35.0	41.0
19	37.837330195606626	38.0	37.0	41.0	34.0	41.0
20	37.24984361169064	38.0	35.0	40.0	34.0	41.0
21	37.15404922031182	38.0	35.0	40.0	33.0	41.0
22	37.15595429199571	38.0	35.0	40.0	33.0	41.0
23	37.08227176246808	38.0	35.0	40.0	33.0	41.0
24	36.98077097354601	38.0	35.0	40.0	33.0	41.0
25	36.918054975205656	38.0	35.0	40.0	33.0	41.0
26	36.869156502447105	38.0	35.0	40.0	33.0	41.0
27	36.787166410387414	38.0	35.0	40.0	33.0	41.0
28	36.63746961021224	38.0	35.0	40.0	33.0	41.0
29	36.50957409964666	38.0	35.0	40.0	33.0	41.0
30	36.29913250412831	38.0	35.0	40.0	32.0	41.0
31	35.877192191802365	38.0	35.0	40.0	31.0	41.0
32	35.181143464291296	38.0	35.0	40.0	24.0	41.0
33	34.2751038139153	38.0	34.0	40.0	18.0	41.0
34	33.46276439666443	38.0	33.0	40.0	13.0	41.0
35	32.919002367501015	38.0	33.0	40.0	10.0	41.0
36	32.629223280377666	38.0	32.0	40.0	8.0	41.0
37	32.42140489361921	38.0	31.0	40.0	8.0	41.0
38	32.299272604540135	38.0	31.0	40.0	8.0	41.0
39	32.173745084851376	38.0	31.0	40.0	8.0	41.0
40	32.00684973447053	38.0	30.0	40.0	7.0	41.0
41	31.8395536773174	37.0	30.0	40.0	7.0	41.0
42	31.70709559599744	37.0	29.0	40.0	7.0	41.0
43	31.561103129088814	37.0	28.0	40.0	7.0	41.0
44	31.415138584290236	37.0	27.0	40.0	7.0	41.0
45	31.38953449923655	37.0	27.0	40.0	7.0	41.0
46	31.249578106714385	37.0	26.0	40.0	7.0	41.0
47	31.159978504873877	37.0	25.0	40.0	7.0	41.0
48	31.078589472972624	36.0	25.0	40.0	7.0	41.0
49	31.068520858061138	36.0	26.0	40.0	7.0	41.0
50	30.97212442680072	36.0	25.0	40.0	7.0	41.0
51	30.19462004621354	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	3.0
11	8.0
12	11.0
13	11.0
14	25.0
15	54.0
16	118.0
17	314.0
18	677.0
19	1557.0
20	2749.0
21	4626.0
22	7232.0
23	12850.0
24	25074.0
25	52705.0
26	87432.0
27	96102.0
28	81984.0
29	65818.0
30	55631.0
31	53442.0
32	54486.0
33	62228.0
34	89139.0
35	122779.0
36	158703.0
37	189635.0
38	353824.0
39	462030.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.588917469590616	3.2459697863174806	41.19010891092504	38.97500383316686
2	42.587292108868795	3.3927813017875534	40.478046118508296	13.541880470835357
3	14.148378092802316	3.3427664344886066	67.72625359252235	14.782601880186716
4	12.05916744105618	3.4211932734167303	68.01292058902916	16.50671869649793
5	12.085717938682066	4.3631970913978835	66.91180972992463	16.639275239995435
6	15.24287582057938	4.997224934150357	67.78518394057392	11.974715304696353
7	61.33468665759116	1.315572258746846	34.29413150726	3.055609576401996
8	63.563311914952195	1.5588865054401577	32.33532200806018	2.542479571547468
9	58.656221511487495	4.384506070119766	33.73691396022226	3.2223584581704747
10	24.550196850875846	26.631667689660933	38.73056290483998	10.087572554623241
11	16.200996084536392	19.454362780709054	50.097408975145896	14.247232159608657
12	14.965418221773074	18.186894929099886	51.41920247595637	15.428484373170672
13	16.07573847857811	17.532194927679285	52.77455149498407	13.617515098758542
14	14.82737522858166	19.139871646468855	51.759068440030894	14.273684684918583
15	13.309000275792068	19.851689508095696	52.75432021173777	14.084990004374465
16	15.209810163941976	20.004183417891603	50.73560064132678	14.05040577683964
17	15.078184357446117	19.565365414694767	50.23060233869715	15.125847889161959
18	15.094545734211884	19.056840108690487	50.80844305824503	15.040171098852598
19	15.164546953967218	20.638358219118025	49.729914817969885	14.467180008944872
20	15.758847022596825	21.74142852454182	48.466365858999225	14.033358593862133
21	16.051441344219363	21.42615361177392	49.06566251574292	13.456742528263788
22	15.501326790088923	19.85923337642482	49.13997451740062	15.499465316085633
23	13.691827100416235	21.266507722912735	48.88475663431784	16.156908542353186
24	13.467323538387758	21.375110035157366	49.46480173097488	15.69276469548
25	13.924364392353652	22.519720602549338	47.91693711108052	15.638977894016486
26	14.115410408480875	22.82015270944889	47.43099442390564	15.633442458164595
27	13.959634426100218	21.824949923900004	48.472195211799004	15.743220438200778
28	13.53869637056657	21.407930761004863	48.42609923713856	16.627273631290006
29	14.277015743661314	20.09760002116202	47.74788587988692	17.877498355289745
30	15.320175977873932	20.666084384535463	46.80108141842359	17.21265821916701
31	14.918734413216859	22.467697302773153	45.183313551089874	17.43025473292012
32	14.364554007973968	23.072578381526732	43.81366057393162	18.74920703656768
33	15.084258641035802	23.35126063428257	42.19795012523311	19.366530599448513
34	14.659744595969517	24.70788329341778	40.53036333523236	20.10200877538034
35	14.46600434115332	25.489751360957936	39.03079906710761	21.013445230781137
36	15.176009714934851	26.21974308719585	38.10990828321641	20.494338914652886
37	15.182132984682518	27.742232877255873	38.273522050874085	18.802112087187524
38	15.429317137856355	26.94865711795818	38.57243558687621	19.049590157309247
39	16.010243985357057	26.316833652314862	37.855131275555465	19.81779108677261
40	16.841833003248272	26.493330779521628	37.87614633732946	18.788689879900637
41	15.921383094778907	26.825995778372906	38.17858687670625	19.07403425014194
42	16.390866432872066	26.839417985659793	38.45516272466889	18.314552856799253
43	16.293481950805162	26.390361875444857	38.57792203657013	18.73823413717986
44	16.59881267350285	26.696427390512262	37.08271753650571	19.622042399479177
45	17.095140426169777	26.01150292961718	36.26469768437532	20.62865895983772
46	17.344137067188925	26.32765959322874	36.750444426918286	19.57775891266405
47	16.15769232088089	26.97883259127468	37.517812591695964	19.345662496148464
48	16.05300890127477	26.15517933097645	38.22478082368265	19.567030944066136
49	17.197227579402888	25.155469818893277	38.14992997428717	19.49737262741667
50	16.40134947068007	25.143174293239962	37.97519634876773	20.480279887312243
51	15.998781224389425	24.668988283980596	37.58267026486325	21.74956022676672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	946.0
1	2354.0
2	3762.0
3	93440.5
4	183119.0
5	118456.5
6	53794.0
7	52106.0
8	50418.0
9	50363.5
10	50309.0
11	49212.5
12	48116.0
13	46523.0
14	44930.0
15	42406.0
16	39882.0
17	37472.5
18	35063.0
19	32891.5
20	30720.0
21	29467.0
22	28214.0
23	27249.5
24	26285.0
25	27191.5
26	29005.0
27	29912.0
28	32894.5
29	35877.0
30	38926.5
31	41976.0
32	46564.5
33	51153.0
34	55842.5
35	60532.0
36	65202.5
37	69873.0
38	74925.5
39	79978.0
40	85515.0
41	91052.0
42	96792.5
43	102533.0
44	109025.5
45	115518.0
46	126828.0
47	138138.0
48	143143.5
49	148149.0
50	139526.5
51	130904.0
52	113487.5
53	96071.0
54	82298.5
55	68526.0
56	59034.5
57	49543.0
58	42676.5
59	35810.0
60	31631.5
61	27453.0
62	22995.5
63	18538.0
64	14985.0
65	11432.0
66	8897.0
67	6362.0
68	4868.5
69	3375.0
70	2622.5
71	1870.0
72	1515.0
73	1160.0
74	1046.5
75	742.5
76	552.0
77	434.5
78	317.0
79	210.5
80	104.0
81	73.0
82	42.0
83	40.5
84	39.0
85	23.5
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2041393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.168844832501485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49217928471745	28.814492771857637
2	8.181833504417744	6.245822669509655
3	3.812843327953698	4.365954760659098
4	2.364888421739005	3.610602368621417
5	1.6843414701083286	3.2144684108756114
6	1.2305490394685867	2.8181181207756136
7	0.9982905151329154	2.667251703890636
8	0.81788061144656	2.497404651985215
9	0.6885150410301822	2.365184138933198
>10	4.580959940142264	28.680715040850547
>50	0.0876071941744326	2.291115688206735
>100	0.05456538769378511	3.9158496382503696
>500	0.0029666052423737866	0.8220635589737707
>1k	0.0023216910592490504	1.454340615381732
>5k	1.2898283662494725E-4	0.282538194092135
>10k+	1.2898283662494725E-4	5.954077667136592
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120941	5.924434932421145	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	5739	0.28113156065490574	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	3318	0.1625360721820835	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2271	0.11124756477562137	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4493078990669607E-4	0.0	0.0	0.1091901461404051	0.0
2	2.4493078990669607E-4	0.0	0.0	0.34456863524074005	0.0
3	2.4493078990669607E-4	0.0	0.0	0.5411011010618729	0.0
4	2.4493078990669607E-4	0.0	0.0	0.8357528413196283	0.0
5	2.4493078990669607E-4	0.0	0.0	1.4801657495641456	0.0
6	2.939169478880353E-4	0.0	0.0	2.391161329543111	0.0
7	2.939169478880353E-4	0.0	0.0	3.255718031755767	0.0
8	2.939169478880353E-4	0.0	0.0	4.405619104209723	0.0
9	2.939169478880353E-4	0.0	0.0	5.146828660625367	0.0
10	2.939169478880353E-4	0.0	0.0	6.031616646084316	0.0
11	2.939169478880353E-4	0.0	0.0	7.509088156959488	0.0
12	2.939169478880353E-4	0.0	0.0	8.762203064280126	0.0
13	2.939169478880353E-4	0.0	0.0	9.286795830102289	0.0
14	2.939169478880353E-4	0.0	0.0	9.534273900224013	0.0
15	3.429031058693745E-4	0.0	0.0	9.767937873795002	0.0
16	3.429031058693745E-4	0.0	0.0	10.183095562686852	0.0
17	3.429031058693745E-4	0.0	0.0	10.735757397032321	0.0
18	3.429031058693745E-4	0.0	0.0	11.304780608143556	0.0
19	3.429031058693745E-4	0.0	0.0	11.678838910489063	0.0
20	3.429031058693745E-4	0.0	0.0	12.040552701023271	0.0
21	3.429031058693745E-4	0.0	0.0	12.430678463186657	0.0
22	3.429031058693745E-4	0.0	0.0	12.822616713195352	0.0
23	3.429031058693745E-4	0.0	0.0	13.162874566533734	0.0
24	3.429031058693745E-4	0.0	0.0	13.409078996547946	0.0
25	3.429031058693745E-4	0.0	0.0	13.618200904970282	0.0
26	3.429031058693745E-4	0.0	0.0	13.808561114885766	0.0
27	3.429031058693745E-4	0.0	0.0	14.00837565329165	0.0
28	3.429031058693745E-4	0.0	0.0	14.211913139704114	0.0
29	3.429031058693745E-4	0.0	0.0	14.424953940764958	0.0
30	3.429031058693745E-4	0.0	0.0	14.660920263761069	0.0
31	3.918892638507137E-4	0.0	0.0	14.87381410634797	0.0
32	4.408754218320529E-4	0.0	0.0	15.08200527776866	0.0
33	4.408754218320529E-4	0.0	0.0	15.28461202717948	0.0
34	4.408754218320529E-4	0.0	0.0	15.504021028777899	0.0
35	4.408754218320529E-4	0.0	0.0	15.730386064809666	0.0
36	5.388477377947313E-4	0.0	0.0	15.945680229137652	0.0
37	5.388477377947313E-4	0.0	0.0	16.172976002171065	0.0
38	5.388477377947313E-4	0.0	0.0	16.40644403111013	0.0
39	5.388477377947313E-4	0.0	0.0	16.69100462282373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTG	45	3.8562575E-10	45.0	1
CGTTTTT	58420	0.0	44.437695	1
CTTCGCG	115	0.0	41.08696	1
GTTTACG	55	6.184564E-11	40.909092	1
GTTTTTT	65050	0.0	40.365105	2
CTGCGCG	150	0.0	39.000004	1
GCGCGAC	35	6.2506733E-6	38.571426	9
CGCGACC	35	6.2506733E-6	38.571426	10
GACCGAT	730	0.0	37.910957	9
CCGTTCG	30	1.14023234E-4	37.499996	1
TGGGCGA	2635	0.0	37.485767	6
TTGGGAC	4705	0.0	36.63124	5
CGGGTAT	160	0.0	36.5625	6
TCTTGCG	425	0.0	36.52941	1
TACGGGA	235	0.0	36.38298	4
GGACCGA	830	0.0	36.3253	8
GGCCGAT	585	0.0	36.153843	8
TTGGGAT	8645	0.0	36.150955	5
GGCGATC	880	0.0	36.051136	8
CTCGTTG	150	0.0	36.000004	1
>>END_MODULE