FastQCFastQC Report
Sat 14 Jan 2017
SRR2933727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062134
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1202715.8323561902378795No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC59120.2866932992715313No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC34430.1669629616698042No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA24000.11638428928478943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC207.03459E-445.00000438
CGTTTTT591150.044.3834041
GTTTTTT664450.039.9307672
CTTCGCG1650.039.5454521
CGAATAT1550.039.19354614
CGGGTCG659.094947E-1238.0769276
ACTGCGG659.094947E-1238.0769272
GGACCGA8700.037.241388
TGGGCGA26900.036.970266
GACCGAT7850.036.68799
CGTTTGG10200.036.617652
ACGGGCG1050.036.428575
TTGGGAT83300.036.221495
CGAGACA2050.036.21951322
CTTTGCG5350.036.1682241
TCGTTTG5250.036.01
TACGAAT1750.036.012
TACTCGA250.00210749636.032
TTTGGGA139150.035.9935344
TTGGGAC49300.035.917855
Produced by FastQC (version 0.11.5)