##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933725.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479800
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11639849937474	33.0	31.0	34.0	30.0	34.0
2	32.336811171321386	34.0	31.0	34.0	30.0	34.0
3	32.44204251771571	34.0	31.0	34.0	30.0	34.0
4	35.94864735306378	37.0	35.0	37.0	35.0	37.0
5	36.036734055856606	37.0	35.0	37.0	35.0	37.0
6	36.027901208837015	37.0	35.0	37.0	35.0	37.0
7	36.05496248436849	37.0	35.0	37.0	35.0	37.0
8	35.97937473947478	37.0	35.0	37.0	35.0	37.0
9	37.80190287619842	39.0	38.0	39.0	35.0	39.0
10	37.11032513547311	39.0	37.0	39.0	33.0	39.0
11	37.08231137974156	39.0	37.0	39.0	33.0	39.0
12	37.167724051688204	39.0	37.0	39.0	34.0	39.0
13	37.20362025844102	39.0	37.0	39.0	34.0	39.0
14	38.38976240100042	40.0	38.0	41.0	34.0	41.0
15	38.41373905794081	40.0	38.0	41.0	34.0	41.0
16	38.53917048770321	40.0	38.0	41.0	34.0	41.0
17	38.56473322217591	40.0	38.0	41.0	34.0	41.0
18	38.267232180075034	39.0	38.0	41.0	34.0	41.0
19	37.9242851187995	39.0	37.0	41.0	34.0	41.0
20	37.374506044185075	39.0	35.0	40.0	34.0	41.0
21	37.3054355981659	39.0	35.0	40.0	33.0	41.0
22	37.313720300125055	39.0	35.0	40.0	33.0	41.0
23	37.182034180908715	38.0	35.0	40.0	33.0	41.0
24	37.07150687786578	38.0	35.0	40.0	33.0	41.0
25	36.98722175906628	38.0	35.0	40.0	33.0	41.0
26	36.9970050020842	38.0	35.0	40.0	33.0	41.0
27	36.91533347228012	38.0	35.0	40.0	33.0	41.0
28	36.86040016673614	38.0	35.0	40.0	33.0	41.0
29	36.65453939141309	38.0	35.0	40.0	33.0	41.0
30	36.3297332221759	38.0	35.0	40.0	31.0	41.0
31	35.916042100875366	38.0	35.0	40.0	30.0	41.0
32	35.40937057107128	38.0	35.0	40.0	25.0	41.0
33	34.609956231763235	38.0	34.0	40.0	20.0	41.0
34	34.02349103793247	38.0	34.0	40.0	15.0	41.0
35	33.611811171321385	38.0	33.0	40.0	12.0	41.0
36	33.31238224260108	38.0	33.0	40.0	10.0	41.0
37	33.19917674030846	38.0	33.0	40.0	10.0	41.0
38	33.12965819091288	38.0	33.0	40.0	10.0	41.0
39	33.0438495206336	38.0	33.0	40.0	10.0	41.0
40	32.892325969153816	38.0	33.0	40.0	10.0	41.0
41	32.841390162567734	38.0	32.0	40.0	10.0	41.0
42	32.72345560650271	38.0	32.0	40.0	9.0	41.0
43	32.681300541892455	38.0	32.0	40.0	9.0	41.0
44	32.56302834514381	38.0	31.0	40.0	8.0	41.0
45	32.48344726969571	38.0	31.0	40.0	8.0	41.0
46	32.27949979157982	37.0	31.0	40.0	8.0	41.0
47	32.23779699874948	37.0	31.0	40.0	8.0	41.0
48	32.16035014589412	37.0	31.0	40.0	8.0	41.0
49	32.168155481450604	37.0	31.0	40.0	8.0	41.0
50	32.00068570237599	37.0	30.0	40.0	8.0	41.0
51	31.209912463526468	36.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	3.0
12	7.0
13	6.0
14	15.0
15	17.0
16	43.0
17	77.0
18	162.0
19	338.0
20	664.0
21	1043.0
22	1767.0
23	3186.0
24	6387.0
25	12324.0
26	18528.0
27	18176.0
28	14456.0
29	11537.0
30	10669.0
31	11171.0
32	12731.0
33	16103.0
34	23536.0
35	30832.0
36	40374.0
37	52604.0
38	92911.0
39	100090.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.783243017924136	4.571696540225094	44.16965402250938	31.47540641934139
2	38.32951229679033	4.254689453939141	42.64589412255106	14.769904126719466
3	16.85493955814923	3.936015006252605	62.76406836181743	16.44497707378074
4	13.138182576073365	4.1661108795331385	63.858065860775326	18.837640683618172
5	12.467278032513548	4.996665277198833	63.86202584410171	18.67403084618591
6	15.966235931638181	6.512296790329304	65.6317215506461	11.88974572738641
7	65.65152146727803	1.7150896206752815	29.098165902459357	3.535223009587328
8	67.07419758232597	1.8286786160900377	27.115256356815337	3.9818674447686537
9	61.645685702375985	4.736140058357649	29.145894122551063	4.4722801167152975
10	27.783243017924136	19.86140058357649	37.753647353063776	14.601709045435598
11	19.75239683201334	20.91725719049604	41.28928720300125	18.041058774489372
12	16.189870779491457	18.402667778240932	47.0208420175073	18.386619424760315
13	18.747394747811587	19.503126302626093	48.54147561483952	13.2080033347228
14	17.7919966652772	20.760525218841185	47.18945393914131	14.258024176740308
15	14.413714047519798	20.950604418507712	48.68695289704043	15.948728636932055
16	14.63651521467278	20.27344726969571	46.635889954147565	18.454147561483953
17	15.192788661942478	20.71717382242601	44.86890370987911	19.221133805752398
18	16.076490204251773	18.994997915798248	47.1394330971238	17.789078782826177
19	16.442476031679867	22.663609837432265	45.68841183826595	15.205502292621926
20	16.25406419341392	23.601292205085453	44.8151313047103	15.329512296790329
21	15.77303042934556	24.087119633180492	45.16381825760733	14.976031679866612
22	14.507294706127553	22.281158816173406	44.20466861192163	19.00687786577741
23	13.603376406836182	23.936015006252607	42.7234264276782	19.737182159233015
24	13.997707378074198	23.47311379741559	43.09045435598166	19.438724468528555
25	13.26448520216757	25.22446852855356	41.67736556898708	19.83368070029179
26	13.478324301792414	25.38557732388495	42.81888286786161	18.317215506461025
27	14.1802834514381	25.073989162150895	43.670279283034596	17.075448103376406
28	13.379949979157981	22.91871613172155	43.439558149228844	20.261775739891622
29	14.735931638182576	22.487494789495624	42.03459774906211	20.741975823259693
30	17.568153397248853	24.231763234681118	39.89516465193831	18.30491871613172
31	15.935806586077533	27.341392246769487	36.93226344310129	19.79053772405169
32	15.573363901625678	26.267611504793663	35.936223426427674	22.22280116715298
33	17.49937473947478	25.343059608170073	35.081909128803666	22.07565652355148
34	16.69591496456857	24.539391413088786	34.11296373488954	24.651729887453104
35	16.389537307211338	25.94622759483118	31.887869945810753	25.77636515214673
36	16.710921217173823	28.263443101292207	32.20216756982076	22.823468111713215
37	16.765527303042933	29.776781992496876	31.57940808670279	21.8782826177574
38	19.636098374322632	26.01083784910379	32.068778657774075	22.2842851187995
39	20.131304710295957	24.69528970404335	34.65923301375573	20.51417257190496
40	18.313672363484784	26.83451438099208	33.7303042934556	21.12150896206753
41	16.081909128803666	27.71842434347645	31.54647769904127	24.653188828678616
42	17.053147144643603	26.281158816173406	33.68924551896624	22.976448520216756
43	17.761567319716548	24.82596915381409	36.05106294289287	21.36140058357649
44	18.78991246352647	24.61796581909129	33.283243017924136	23.30887869945811
45	20.26052521884118	24.129637348895372	32.2180075031263	23.39182992913714
46	19.843268028345143	26.0154230929554	33.34576907044602	20.795539808253437
47	16.082325969153814	27.613588995414755	35.396206752813676	20.907878282617755
48	16.765527303042933	26.04210087536474	35.97498957899125	21.217382242601087
49	19.706544393497293	23.007294706127553	36.74551896623593	20.540641934139224
50	18.415381408920382	22.754272613588995	34.58711963318049	24.243226344310127
51	17.110671112963736	22.713213839099623	32.771154647769904	27.404960400166733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	431.0
1	861.0
2	1291.0
3	14159.5
4	27028.0
5	17938.0
6	8848.0
7	8697.5
8	8547.0
9	8679.0
10	8811.0
11	8995.0
12	9179.0
13	8903.0
14	8627.0
15	8261.0
16	7895.0
17	7574.5
18	7254.0
19	6723.5
20	6193.0
21	5924.0
22	5655.0
23	5574.0
24	5493.0
25	5364.0
26	5323.0
27	5411.0
28	5522.5
29	5634.0
30	6232.5
31	6831.0
32	7549.0
33	8267.0
34	9251.0
35	10235.0
36	11262.5
37	12290.0
38	13768.5
39	15247.0
40	17603.5
41	19960.0
42	22961.0
43	25962.0
44	28998.5
45	32035.0
46	37547.0
47	43059.0
48	48139.5
49	53220.0
50	50022.5
51	46825.0
52	38887.0
53	30949.0
54	24708.5
55	18468.0
56	15036.5
57	11605.0
58	9843.0
59	8081.0
60	6887.0
61	5693.0
62	4750.0
63	3807.0
64	3139.0
65	2471.0
66	1901.0
67	1331.0
68	1049.5
69	768.0
70	638.0
71	508.0
72	393.5
73	279.0
74	221.0
75	120.5
76	78.0
77	61.0
78	44.0
79	43.5
80	43.0
81	36.5
82	30.0
83	22.0
84	14.0
85	9.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	479800.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.150970139522855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99465520933192	38.613566543060635
2	9.76081119072602	9.790283011272424
3	4.427077445571107	6.66066686334575
4	2.447350669945916	4.909480414775956
5	1.5772810054849464	3.9551086303856064
6	1.074942101560474	3.234563354224517
7	0.7342719409994429	2.5777115131146786
8	0.5369039186002132	2.154100191160968
9	0.4371561116765934	1.9731422792702478
>10	1.9017875107069329	15.730813360794476
>50	0.0735126728329347	2.47995559603655
>100	0.03132642307640267	2.678536379921502
>500	0.0012530569230561066	0.52033192834324
>1k	0.0012530569230561066	1.0249533516036529
>5k	0.0	0.0
>10k+	4.176856410187022E-4	3.696786582689815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17648	3.6781992496873697	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC	2487	0.5183409754064194	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC	1372	0.2859524802000834	No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	1034	0.21550646102542725	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	897	0.1869528970404335	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	842	0.17548978741142143	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	745	0.15527303042934557	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG	488	0.10170904543559817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22926219258024177	0.0
2	0.0	0.0	0.0	0.7982492705293872	0.0
3	0.0	0.0	0.0	1.161317215506461	0.0
4	0.0	0.0	0.0	1.752396832013339	0.0
5	0.0	0.0	0.0	3.024176740308462	0.0
6	0.0	0.0	0.0	4.232596915381409	0.0
7	0.0	0.0	0.0	5.353063776573572	0.0
8	0.0	0.0	0.0	7.155481450604419	0.0
9	0.0	0.0	0.0	8.017924135056273	0.0
10	0.0	0.0	0.0	9.896206752813672	0.0
11	0.0	0.0	0.0	13.783451438099208	0.0
12	0.0	0.0	0.0	17.073780741975824	0.0
13	0.0	0.0	0.0	18.15756565235515	0.0
14	0.0	0.0	0.0	18.545227177990828	0.0
15	0.0	0.0	0.0	19.046269278866195	0.0
16	0.0	0.0	0.0	20.378907878282618	0.0
17	0.0	0.0	0.0	22.25614839516465	0.0
18	0.0	0.0	0.0	24.097123801583994	0.0
19	0.0	0.0	0.0	25.190496040016672	0.0
20	0.0	0.0	0.0	26.15193830762818	0.0
21	0.0	0.0	0.0	27.348895373072114	0.0
22	0.0	0.0	0.0	28.434556065027095	0.0
23	0.0	0.0	0.0	29.215506461025427	0.0
24	0.0	0.0	0.0	29.677157148812004	0.0
25	0.0	0.0	0.0	30.025218841183825	0.0
26	0.0	0.0	0.0	30.314506044185077	0.0
27	0.0	0.0	0.0	30.588161734055856	0.0
28	0.0	0.0	0.0	30.84493538974573	0.0
29	0.0	0.0	0.0	31.095664860358482	0.0
30	2.0842017507294707E-4	0.0	0.0	31.351187994997915	0.0
31	4.1684035014589413E-4	0.0	0.0	31.58128386827845	0.0
32	4.1684035014589413E-4	0.0	0.0	31.794706127553148	0.0
33	4.1684035014589413E-4	0.0	0.0	32.00979574822843	0.0
34	4.1684035014589413E-4	0.0	0.0	32.22759483117966	0.0
35	6.252605252188412E-4	0.0	0.0	32.45810754481034	0.0
36	6.252605252188412E-4	0.0	0.0	32.6625677365569	0.0
37	6.252605252188412E-4	0.0	0.0	32.891413088787	0.0
38	6.252605252188412E-4	0.0	0.0	33.149020425177156	0.0
39	6.252605252188412E-4	0.0	0.0	33.531263026260945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTG	30	2.1628475E-6	45.000004	1
GCTAGCG	30	2.1628475E-6	45.000004	1
TATCCGG	30	2.1628475E-6	45.000004	2
CGCATGG	25	3.8873975E-5	45.0	2
TAGCGGA	25	3.8873975E-5	45.0	3
TACGGGT	25	3.8873975E-5	45.0	4
CACTGCG	25	3.8873975E-5	45.0	1
CCTCGTT	20	7.029174E-4	45.0	14
TTATCCG	20	7.029174E-4	45.0	1
TCGGAAT	20	7.029174E-4	45.0	5
CCTCCGC	25	3.8873975E-5	45.0	45
TATTCGG	20	7.029174E-4	45.0	2
CGGGCTA	20	7.029174E-4	45.0	6
TTGGTCG	20	7.029174E-4	45.0	1
CTAGCGG	35	1.2099554E-7	45.0	2
CGTTTTT	10235	0.0	44.362484	1
GGACCGA	190	0.0	41.44737	8
CCGCTTG	50	1.0786607E-9	40.5	1
GCGTTTG	125	0.0	39.6	1
GTTTTTT	11870	0.0	39.408173	2
>>END_MODULE