FastQCFastQC Report
Sat 14 Jan 2017
SRR2933724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92287
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65417.087672153174336No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC19912.1574002838969735No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT13311.4422399687930043Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC11631.2601991613119943No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC10271.1128327933511764No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC7530.8159329049595284No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC5140.5569581847930911No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG3870.4193440029473273No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG3480.37708452978209284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.27847909239654556No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG2410.26114187263644933No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT2060.22321670441123884No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.2026287559461246No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG1710.18529153618602837No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1660.1798736550109983No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC1620.17553935007097424No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1500.16253643525090206No Hit
GGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1490.16145285901589607No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1440.156034977840866No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGT1430.15495140160586No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1210.1311127244357277No Hit
TTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1150.12461126702569159No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1120.12136053832067355No Hit
GTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1100.11919338585066153No Hit
TGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1050.11377550467563145No Hit
GTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC980.10619047103058936No Hit
GTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG940.1018561660905653No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA930.10077258985555929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATG302.1437845E-645.0000048
TTCCTTG302.1437845E-645.0000041
TTGGGTA302.1437845E-645.0000045
GTCTTTG302.1437845E-645.0000041
TTAGGGA302.1437845E-645.0000044
TGGGCTC302.1437845E-645.0000046
GCTTTGG600.045.0000042
CGCTGGG253.8628787E-545.03
GTGTTTG253.8628787E-545.01
GGGTTCG206.9995614E-445.07
TGTCTTA206.9995614E-445.030
ATGATCT253.8628787E-545.011
CCTCGGG406.697519E-945.03
GGCCGGT206.9995614E-445.09
TGTTCGG206.9995614E-445.02
AGGGCCT206.9995614E-445.06
TTACACG206.9995614E-445.034
AAGGGGC253.8628787E-545.05
TACACGC206.9995614E-445.035
TTGGGCA406.697519E-945.05
Produced by FastQC (version 0.11.5)