##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933724.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92287
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936469925341598	33.0	31.0	34.0	30.0	34.0
2	32.148840031640425	33.0	31.0	34.0	30.0	34.0
3	32.23638215566656	33.0	31.0	34.0	30.0	34.0
4	35.86690433105421	37.0	35.0	37.0	35.0	37.0
5	35.89929242471854	37.0	35.0	37.0	35.0	37.0
6	35.87126030751894	37.0	35.0	37.0	35.0	37.0
7	35.870718519401436	37.0	35.0	37.0	35.0	37.0
8	35.65951867543641	37.0	35.0	37.0	33.0	37.0
9	37.48660157985415	39.0	37.0	39.0	34.0	39.0
10	37.11480490209889	39.0	37.0	39.0	33.0	39.0
11	37.110665640881166	39.0	37.0	39.0	33.0	39.0
12	37.15872224690368	39.0	37.0	39.0	34.0	39.0
13	37.066130657622416	39.0	37.0	39.0	33.0	39.0
14	38.09481292056303	40.0	37.0	41.0	33.0	41.0
15	38.13150281188033	40.0	37.0	41.0	33.0	41.0
16	38.189777541798954	40.0	37.0	41.0	33.0	41.0
17	38.173718941996164	40.0	37.0	41.0	33.0	41.0
18	37.863382708290445	39.0	37.0	40.0	33.0	41.0
19	37.5137993433528	38.0	37.0	40.0	33.0	41.0
20	36.99840714293454	38.0	35.0	40.0	33.0	41.0
21	36.86989500146283	38.0	35.0	40.0	33.0	41.0
22	36.85759641119551	38.0	35.0	40.0	33.0	41.0
23	36.83369271945128	38.0	35.0	40.0	33.0	41.0
24	36.7027642029755	38.0	35.0	40.0	32.0	41.0
25	36.574078689306184	38.0	35.0	40.0	32.0	41.0
26	36.451407023741154	38.0	35.0	40.0	32.0	41.0
27	36.350536912024445	38.0	35.0	40.0	32.0	41.0
28	36.273050375459164	38.0	35.0	40.0	31.0	41.0
29	36.20300800762838	38.0	35.0	40.0	31.0	41.0
30	36.09737016047764	38.0	35.0	40.0	31.0	41.0
31	35.80717760898068	38.0	35.0	40.0	30.0	41.0
32	35.382675783154724	38.0	35.0	40.0	28.0	41.0
33	34.89910821675859	38.0	35.0	40.0	24.0	41.0
34	34.47023957870556	38.0	34.0	40.0	21.0	41.0
35	34.031932991645625	38.0	34.0	40.0	18.0	41.0
36	33.75112421034382	37.0	33.0	40.0	18.0	41.0
37	33.54132217972195	37.0	33.0	40.0	15.0	41.0
38	33.57600745500449	37.0	33.0	40.0	15.0	41.0
39	33.59041901893008	37.0	33.0	40.0	15.0	41.0
40	33.40143248778268	37.0	33.0	40.0	15.0	41.0
41	33.21494901773814	37.0	33.0	40.0	15.0	41.0
42	33.032669823485435	37.0	33.0	40.0	13.0	41.0
43	32.66905414630446	37.0	31.0	40.0	12.0	41.0
44	32.45908957924735	36.0	31.0	40.0	10.0	41.0
45	32.57467465623544	37.0	31.0	40.0	10.0	41.0
46	32.34770877805108	36.0	31.0	40.0	10.0	41.0
47	32.12897807925277	36.0	31.0	40.0	10.0	41.0
48	32.08508240597267	36.0	31.0	39.0	10.0	41.0
49	32.08926501023979	36.0	31.0	39.0	10.0	41.0
50	31.999555733743648	36.0	31.0	39.0	10.0	41.0
51	30.845882952095096	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	1.0
15	3.0
16	8.0
17	26.0
18	40.0
19	81.0
20	150.0
21	202.0
22	333.0
23	578.0
24	922.0
25	1575.0
26	2416.0
27	2752.0
28	2633.0
29	2513.0
30	2530.0
31	2790.0
32	3111.0
33	4056.0
34	5546.0
35	8202.0
36	10727.0
37	12133.0
38	17918.0
39	11036.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.838113710490102	4.266039637218676	38.11912837127656	37.77671828101466
2	40.37621766879409	4.143595522662997	37.905663852980375	17.57452295556254
3	16.088939937369293	4.263872484748664	61.776848310162855	17.870339267719178
4	13.990052770162645	4.233532350168496	62.678383737687874	19.098031141980996
5	11.719960557825045	5.120981286638422	62.72281036332311	20.436247792213422
6	14.840660114642365	6.123289304018984	65.11426311398138	13.921787467357266
7	52.831926490188216	2.4683866633436997	37.32378341478215	7.375903431685936
8	50.65827256276615	3.1770455210376323	37.012797035335424	9.151884880860793
9	45.92196083955486	4.925937564337339	40.387053431144146	8.765048164963646
10	23.832175712722268	17.450995264771855	44.22616403177045	14.490664990735425
11	14.94793416190796	16.52778831254673	46.37489570578738	22.14938181975793
12	13.931539653472319	14.714965271381669	52.83301006642323	18.52048500872279
13	17.074994311224767	16.842025420698473	51.4059401649203	14.677040103156457
14	13.711573677766097	19.346170099797373	50.05688775233782	16.88536847009871
15	9.833454332679576	18.067550142490273	51.457951824200585	20.64104370062956
16	9.589649679803221	19.331000032507287	47.6578499680345	23.42150031965499
17	9.455286226662476	18.748036018074053	46.41607160271761	25.380606152545866
18	10.593041273418793	17.654707596952985	51.19897710403415	20.55327402559407
19	10.815174401595025	18.814134168409417	51.1155417339387	19.25514969605687
20	11.674450355954793	23.25246242699405	48.51495876992426	16.558128447126897
21	11.98543673540152	23.555863772795735	48.504123007574194	15.954576484228546
22	9.591816832273233	23.626296228071126	44.673681016827935	22.1082059228277
23	10.863935332170295	21.88498921841646	42.95729626057842	24.29377918883483
24	13.132943968272887	22.006349756737134	41.88130505921744	22.979401215772537
25	10.230043234691777	24.924420557608332	39.49201946102918	25.353516746670714
26	10.208371709991656	28.118803298406057	40.895250685361965	20.777574306240314
27	11.86841050202087	26.029668317314464	44.33018735033104	17.771733830333634
28	9.10420752652053	22.271825934313608	43.9996965986542	24.624269940511663
29	10.941952821090728	21.676942581295307	43.78514850412301	23.59595609349096
30	15.679348120537021	23.314226272389394	41.502053376965335	19.50437223010825
31	14.685708713036504	27.0991580612654	36.80475039821427	21.41038282748383
32	15.629503613726744	25.416364168301058	35.10461928548983	23.849512932482362
33	19.12620412409115	20.61612144722442	36.98462405322526	23.273050375459164
34	16.059683379024133	19.453444147062967	37.24901665456673	27.23785581934617
35	17.518176991342227	25.474877284991386	32.60264175886095	24.404303964805443
36	19.132705581501185	29.0810189950914	34.544410371991724	17.24186505141569
37	16.385839825760943	25.525805368036668	38.874381007075755	19.213973799126638
38	17.352389827386304	21.138405192497316	37.39855017499757	24.110654805118813
39	19.620314887253894	17.537681363572332	39.33490090695331	23.507102842220466
40	19.71892032463944	18.543240109657916	36.8654306673746	24.87240889832804
41	22.8287841191067	21.744124307865683	30.812573818631012	24.614517754396612
42	26.74374505618343	20.188108834397045	33.612534809886554	19.45561129953298
43	22.81578120428663	18.313521947836637	39.3869125662336	19.483784281643135
44	17.37081062338141	17.53876493980734	38.303336331227584	26.787088105583667
45	20.399406200223215	15.88089330024814	36.44391951195726	27.27578098757138
46	21.935917301461743	16.748837864487957	40.977602479222426	20.337642354827874
47	17.48783685676206	20.459002893148547	41.70576570914647	20.34739454094293
48	17.64495541083793	18.08813809095539	42.5769610020913	21.689945496115378
49	22.746432325246243	14.19268152610877	42.74924962345726	20.31163652518773
50	20.729896951900052	12.995329786427124	38.37701951520799	27.897753746464833
51	17.90284655476936	13.052759326882443	36.32689327857662	32.71750083977158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	361.0
1	523.0
2	685.0
3	4899.5
4	9114.0
5	5459.5
6	1805.0
7	1712.0
8	1619.0
9	1592.0
10	1565.0
11	1489.5
12	1414.0
13	1344.0
14	1274.0
15	1178.5
16	1083.0
17	1011.0
18	939.0
19	824.5
20	710.0
21	670.5
22	631.0
23	575.0
24	519.0
25	500.0
26	482.5
27	484.0
28	460.5
29	437.0
30	494.0
31	551.0
32	581.5
33	612.0
34	716.5
35	821.0
36	937.5
37	1054.0
38	1260.5
39	1467.0
40	2126.5
41	2786.0
42	3812.5
43	4839.0
44	5148.0
45	5457.0
46	7275.0
47	9093.0
48	11355.0
49	13617.0
50	12948.0
51	12279.0
52	9268.0
53	6257.0
54	4733.5
55	3210.0
56	2579.5
57	1949.0
58	1671.5
59	1394.0
60	1264.0
61	1134.0
62	983.0
63	832.0
64	752.5
65	673.0
66	561.5
67	450.0
68	356.0
69	262.0
70	213.0
71	164.0
72	136.5
73	109.0
74	94.5
75	60.0
76	40.0
77	31.5
78	23.0
79	15.5
80	8.0
81	6.0
82	4.0
83	2.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	92287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.05109061948053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06672571597284	45.17862754234074
2	10.585572286192303	11.654945983724685
3	3.326444247613424	5.49373151148049
4	1.3266410786339926	2.9213215295762134
5	0.7400846373388446	2.0371233218113063
6	0.4192500738116327	1.3848104283376856
7	0.2854049798248204	1.099829878531104
8	0.17124298789489226	0.7541690595641857
9	0.16140143686645014	0.7996792614344381
>10	0.7971656333038087	8.02171486774952
>50	0.070859167404783	2.673182571759836
>100	0.0354295837023915	3.5476285934096894
>500	0.003936620411376833	1.3728910897526196
>1k	0.007873240822753666	5.972672207353148
>5k	0.0019683102056884164	7.087672153174336
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6541	7.087672153174336	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	1991	2.1574002838969735	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	1331	1.4422399687930043	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1163	1.2601991613119943	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	1027	1.1128327933511764	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	753	0.8159329049595284	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	514	0.5569581847930911	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	387	0.4193440029473273	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	348	0.37708452978209284	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	257	0.27847909239654556	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	241	0.26114187263644933	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	206	0.22321670441123884	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	187	0.2026287559461246	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG	171	0.18529153618602837	No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	166	0.1798736550109983	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	162	0.17553935007097424	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	150	0.16253643525090206	No Hit
GGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	149	0.16145285901589607	No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	144	0.156034977840866	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGT	143	0.15495140160586	No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	121	0.1311127244357277	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	115	0.12461126702569159	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	112	0.12136053832067355	No Hit
GTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	110	0.11919338585066153	No Hit
TGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	105	0.11377550467563145	No Hit
GTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	98	0.10619047103058936	No Hit
GTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	94	0.1018561660905653	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	93	0.10077258985555929	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5668512358186961	0.0
2	0.0	0.0	0.0	4.541268000910204	0.0
3	0.0	0.0	0.0	6.227312622579562	0.0
4	0.0	0.0	0.0	8.08456228937987	0.0
5	0.0	0.0	0.0	11.351544637923	0.0
6	0.0	0.0	0.0	13.468852601124752	0.0
7	0.0	0.0	0.0	15.645757257251834	0.0
8	0.0	0.0	0.0	18.341694929946797	0.0
9	0.0	0.0	0.0	19.76226337403968	0.0
10	0.0	0.0	0.0	22.661913378915774	0.0
11	0.0	0.0	0.0	29.852525274415683	0.0
12	0.0	0.0	0.0	35.203224722875376	0.0
13	0.0	0.0	0.0	37.08973094802085	0.0
14	0.0	0.0	0.0	37.89699524310033	0.0
15	0.0	0.0	0.0	38.926392666356044	0.0
16	0.0	0.0	0.0	41.31351111207429	0.0
17	0.0	0.0	0.0	44.09938561227475	0.0
18	0.0	0.0	0.0	46.36839424837734	0.0
19	0.0	0.0	0.0	47.77595977765016	0.0
20	0.0	0.0	0.0	48.847616674071105	0.0
21	0.0	0.0	0.0	49.99404033070747	0.0
22	0.0	0.0	0.0	50.871737081062335	0.0
23	0.0	0.0	0.0	51.48829195878076	0.0
24	0.0	0.0	0.0	51.89354947067301	0.0
25	0.0	0.0	0.0	52.19044935906466	0.0
26	0.0	0.0	0.0	52.41691679218091	0.0
27	0.0	0.0	0.0	52.596790447191914	0.0
28	0.0	0.0	0.0	52.75065827256277	0.0
29	0.0	0.0	0.0	52.87526953958846	0.0
30	0.0	0.0	0.0	53.03997312730937	0.0
31	0.0	0.0	0.0	53.167835123040085	0.0
32	0.0	0.0	0.0	53.29894784747581	0.0
33	0.0	0.0	0.0	53.39972043733137	0.0
34	0.0	0.0	0.0	53.506994484596966	0.0
35	0.0	0.0	0.0	53.62943859915264	0.0
36	0.0	0.0	0.0	53.7291276127732	0.0
37	0.0	0.0	0.0	53.84073596497882	0.0
38	0.0	0.0	0.0	53.92958921624931	0.0
39	0.0	0.0	0.0	54.04553187339495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGATG	30	2.1437845E-6	45.000004	8
TTCCTTG	30	2.1437845E-6	45.000004	1
TTGGGTA	30	2.1437845E-6	45.000004	5
GTCTTTG	30	2.1437845E-6	45.000004	1
TTAGGGA	30	2.1437845E-6	45.000004	4
TGGGCTC	30	2.1437845E-6	45.000004	6
GCTTTGG	60	0.0	45.000004	2
CGCTGGG	25	3.8628787E-5	45.0	3
GTGTTTG	25	3.8628787E-5	45.0	1
GGGTTCG	20	6.9995614E-4	45.0	7
TGTCTTA	20	6.9995614E-4	45.0	30
ATGATCT	25	3.8628787E-5	45.0	11
CCTCGGG	40	6.697519E-9	45.0	3
GGCCGGT	20	6.9995614E-4	45.0	9
TGTTCGG	20	6.9995614E-4	45.0	2
AGGGCCT	20	6.9995614E-4	45.0	6
TTACACG	20	6.9995614E-4	45.0	34
AAGGGGC	25	3.8628787E-5	45.0	5
TACACGC	20	6.9995614E-4	45.0	35
TTGGGCA	40	6.697519E-9	45.0	5
>>END_MODULE