FastQCFastQC Report
Sat 14 Jan 2017
SRR2933723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92108
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65447.104703174534242No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC19532.1203369957006992No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT13941.513440743475051Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC11541.2528770573674382No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC9721.0552829287358318No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC7410.8044903808572544No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC4910.5330698744951579No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG3640.39518825726321277No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG3140.3409041559907934No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG2950.32027619750727404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.22473617926781603No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1990.21605072306422896No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC1890.20519390280974512No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.19867981065705476No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1680.18239458027532895No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG1610.17479480609719025No Hit
GGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1460.15850957571546445No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1440.15633821166456768No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1370.14873843748642898No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1350.1465670734355322No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGT1230.13353888913015155No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.13245320710470318No Hit
TTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1100.11942502279932254No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT1100.11942502279932254No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT1040.1129109306466322No Hit
GTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC1030.11182524862118383No Hit
GTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG1020.11073956659573543No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.10748252051939028No Hit
TGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT970.10531115646849351No Hit
GTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC940.10205411039214835No Hit
TTGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT930.10096842836669996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG351.1957309E-745.00000414
AATCCTA351.1957309E-745.00000413
TCCTAGC351.1957309E-745.00000415
GGGTATG351.1957309E-745.0000047
GGGTAGA351.1957309E-745.0000047
TGGGCAT351.1957309E-745.0000046
TTCGGGC351.1957309E-745.0000044
ATGGGTA206.9995027E-445.05
GCCCTAC253.86283E-545.026
ACTAATC406.697519E-945.010
GCCTTTG206.9995027E-445.01
GGCATGG206.9995027E-445.02
TATACTA253.86283E-545.044
ACATGAT206.9995027E-445.032
ATGATTT206.9995027E-445.034
CACAATT206.9995027E-445.043
GGGTACT206.9995027E-445.07
GGACTAA406.697519E-945.08
CAACTAG206.9995027E-445.017
TATGCCA206.9995027E-445.010
Produced by FastQC (version 0.11.5)