##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92108
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83600773005602	33.0	31.0	34.0	30.0	34.0
2	32.08258783167586	33.0	31.0	34.0	30.0	34.0
3	32.17556564033526	33.0	31.0	34.0	30.0	34.0
4	35.78404698831806	37.0	35.0	37.0	35.0	37.0
5	35.87255178703261	37.0	35.0	37.0	35.0	37.0
6	35.87407174186824	37.0	35.0	37.0	35.0	37.0
7	35.80763885873105	37.0	35.0	37.0	35.0	37.0
8	35.62385460546315	37.0	35.0	37.0	33.0	37.0
9	37.45162200894602	39.0	37.0	39.0	34.0	39.0
10	37.12833847222825	39.0	37.0	39.0	33.0	39.0
11	37.13529769401138	39.0	37.0	39.0	34.0	39.0
12	37.154318843097236	39.0	37.0	39.0	34.0	39.0
13	37.10350892430625	39.0	37.0	39.0	33.0	39.0
14	38.151756633517174	40.0	37.0	41.0	33.0	41.0
15	38.16628305901767	40.0	37.0	41.0	33.0	41.0
16	38.29468667216746	40.0	38.0	41.0	33.0	41.0
17	38.326421157771314	40.0	38.0	41.0	34.0	41.0
18	38.06381638945586	39.0	38.0	40.0	34.0	41.0
19	37.74908802709862	39.0	37.0	40.0	34.0	41.0
20	37.205335041473056	38.0	35.0	40.0	33.0	41.0
21	37.112552655578234	38.0	35.0	40.0	33.0	41.0
22	37.085432318582534	38.0	35.0	40.0	33.0	41.0
23	36.98405133104616	38.0	35.0	40.0	33.0	41.0
24	36.866157119902724	38.0	35.0	40.0	33.0	41.0
25	36.749522299908804	38.0	35.0	40.0	33.0	41.0
26	36.70542189603509	38.0	35.0	40.0	33.0	41.0
27	36.64824988057498	38.0	35.0	40.0	33.0	41.0
28	36.6136817648847	38.0	35.0	40.0	33.0	41.0
29	36.47651669778955	38.0	35.0	40.0	32.0	41.0
30	36.247861206409866	38.0	35.0	40.0	31.0	41.0
31	35.96683241412255	38.0	35.0	40.0	31.0	41.0
32	35.62855560863334	38.0	35.0	40.0	30.0	41.0
33	35.07136187953272	38.0	35.0	40.0	25.0	41.0
34	34.642148347591956	38.0	35.0	40.0	21.0	41.0
35	34.32173101142138	38.0	34.0	40.0	20.0	41.0
36	34.009597429104964	38.0	34.0	40.0	18.0	41.0
37	33.8253029052851	37.0	33.0	40.0	17.0	41.0
38	33.787705736743824	38.0	33.0	40.0	16.0	41.0
39	33.82811482173101	38.0	33.0	40.0	15.0	41.0
40	33.617155947366136	38.0	33.0	40.0	15.0	41.0
41	33.50702436270465	37.0	33.0	40.0	15.0	41.0
42	33.250597125114	37.0	33.0	40.0	15.0	41.0
43	32.887512485343294	37.0	32.0	40.0	12.0	41.0
44	32.6861944673644	37.0	32.0	40.0	10.0	41.0
45	32.73097885091414	37.0	32.0	40.0	10.0	41.0
46	32.51107395665957	36.0	31.0	40.0	10.0	41.0
47	32.44562904416554	36.0	31.0	40.0	10.0	41.0
48	32.33403178876971	36.0	31.0	40.0	10.0	41.0
49	32.326594866895384	36.0	31.0	40.0	10.0	41.0
50	32.15500282277327	36.0	31.0	39.0	10.0	41.0
51	31.148271594215487	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	2.0
13	4.0
14	4.0
15	4.0
16	15.0
17	29.0
18	52.0
19	81.0
20	154.0
21	207.0
22	336.0
23	542.0
24	898.0
25	1528.0
26	2362.0
27	2611.0
28	2462.0
29	2435.0
30	2374.0
31	2745.0
32	3055.0
33	3733.0
34	5413.0
35	7899.0
36	10424.0
37	10927.0
38	18150.0
39	13653.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.83975333304382	4.449124940287488	37.8881313240978	37.822990402570895
2	40.413427715290744	4.025708950362618	38.01407043904981	17.546792895296825
3	15.987753506752941	4.231988535197812	62.12272549615668	17.65753246189256
4	14.008555174360534	4.180961480001737	62.52225648152169	19.288226864116037
5	11.789421114344032	5.067963694793069	62.597168541277625	20.54544664958527
6	14.868415338515655	6.025535241238546	65.23646154514266	13.86958787510314
7	52.841229860598425	2.370043861553828	37.304034394406564	7.484691883441178
8	50.76649150996656	3.1506492378512183	36.92404568549963	9.158813566682591
9	45.916749902288615	4.984366178833543	40.421027489468884	8.677856429408955
10	24.00551526468928	17.419768098319363	43.953836800277934	14.620879836713424
11	15.220176314760932	16.552308159986104	46.31519520562817	21.91232031962479
12	13.865245147001346	14.933556260042558	52.60780822512703	18.59339036782907
13	16.92578277674035	16.617449081513005	51.67086463716507	14.785903504581576
14	13.69479306900595	19.497763495027577	49.968515221261995	16.838928214704477
15	9.981760541972468	17.89421114344031	51.49172710296608	20.63230121162114
16	9.68971207712685	19.197029573978373	47.8796630042993	23.233595344595475
17	9.573544100403874	19.03634863421201	46.309766795500934	25.080340469883183
18	10.729795457506405	17.572849263907585	51.041169062405004	20.656186216181005
19	10.71351022712468	19.250227993225344	50.98797064315803	19.048291136491944
20	12.001129109306467	22.884005732401093	48.369305597776524	16.745559560515915
21	11.874104312329004	23.70260997958918	48.62986928388413	15.793416424197682
22	9.751595952577409	23.42576106309984	44.643244886437664	22.17939809788509
23	11.199895774525558	21.887349633039477	42.97563729534894	23.93711729708603
24	13.16280887653624	22.053458982933076	41.80310070786468	22.980631432666
25	10.317236287836018	25.041255916967035	39.473227081252446	25.1682807139445
26	10.128327615407999	28.032309897077347	41.23746037260607	20.601902114908587
27	11.93055977765232	25.797976288704564	44.41633734311895	17.85512659052417
28	9.264124723151083	22.29339471055717	44.19377252790203	24.248708038389715
29	10.82642115777131	21.904720545446647	43.63247492074521	23.636383376036825
30	16.011638511312807	23.11634168584705	41.34168584704911	19.530333955791026
31	14.873843748642898	27.29513180179789	36.66022495331568	21.17079949624354
32	15.805358926477615	25.476614409171844	34.68645503105051	24.03157163330004
33	19.075433187128155	20.94823468102662	36.8154774829548	23.16085464889043
34	15.867242801928171	19.50210622312937	37.56459808051418	27.066052894428278
35	17.58913449428931	25.53306987449516	32.460806878881314	24.416988752334216
36	19.087375689408088	28.968167803013856	35.0610153298302	16.88344117774786
37	16.54687974985886	25.39084552916142	38.966213575368045	19.096061145611674
38	17.378512181352328	21.370564988926045	37.503799887089066	23.74712294263256
39	19.90163720849438	17.49142311199896	39.24631953793373	23.360620141572934
40	19.903808572545273	18.656359925305075	36.65262517913753	24.787206323012118
41	22.742867069092803	21.780952794545534	30.753029052850998	24.72315108351066
42	26.97159855821427	20.57910279237417	33.361922960003476	19.087375689408088
43	22.659269553133278	18.623789464541627	39.544882094932035	19.17205888739306
44	17.16680418638989	17.877925913058583	38.10852477526382	26.846745125287708
45	20.20128544751813	16.13866330829027	36.51908629000739	27.14096495418422
46	21.681070048204283	16.955096191427454	41.107178529552264	20.256655230815998
47	17.349198766665218	20.492248230338298	41.52625179137534	20.63230121162114
48	17.865983410778654	17.959352064967213	42.55547835150041	21.619186172753725
49	23.266165805358927	14.074781777912884	42.71833065531767	19.94072176141052
50	20.54436096755982	13.035784079558779	38.61553828114822	27.804316671733183
51	17.91483910192383	13.224692751986797	36.36491944239371	32.49554870369566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309.0
1	512.5
2	716.0
3	4876.0
4	9036.0
5	5446.5
6	1857.0
7	1749.0
8	1641.0
9	1655.5
10	1670.0
11	1552.0
12	1434.0
13	1342.0
14	1250.0
15	1161.5
16	1073.0
17	974.5
18	876.0
19	811.5
20	747.0
21	681.5
22	616.0
23	586.0
24	556.0
25	519.0
26	482.0
27	482.0
28	481.0
29	480.0
30	506.0
31	532.0
32	595.5
33	659.0
34	715.0
35	771.0
36	887.0
37	1003.0
38	1217.0
39	1431.0
40	2115.0
41	2799.0
42	3842.0
43	4885.0
44	5164.5
45	5444.0
46	7176.0
47	8908.0
48	11316.0
49	13724.0
50	12826.5
51	11929.0
52	9182.0
53	6435.0
54	4791.5
55	3148.0
56	2542.0
57	1936.0
58	1693.5
59	1451.0
60	1292.5
61	1134.0
62	997.5
63	861.0
64	766.5
65	672.0
66	586.5
67	501.0
68	375.0
69	249.0
70	207.0
71	165.0
72	137.0
73	109.0
74	92.0
75	54.5
76	34.0
77	25.5
78	17.0
79	13.0
80	9.0
81	5.0
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	92108.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.330698744951576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20312377364414	45.48356277413471
2	10.609449807707401	11.740565423198854
3	3.2454281453575073	5.387154210274895
4	1.3480103602542972	2.9834542059321665
5	0.671061926065458	1.8565162635167411
6	0.3688878423985559	1.2246493247057801
7	0.2727415430499961	1.0563686107612802
8	0.18836825994819872	0.8338037955443609
9	0.14520053371006986	0.7230642289486255
>10	0.8201867985244486	7.857080818169974
>50	0.07456243622949534	2.7608893907152474
>100	0.04120555686366847	4.241759673426847
>500	0.003924338748920807	1.8597733095930862
>1k	0.00588650812338121	4.886654796543189
>5k	0.0019621693744604034	7.104703174534242
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6544	7.104703174534242	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	1953	2.1203369957006992	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	1394	1.513440743475051	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1154	1.2528770573674382	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	972	1.0552829287358318	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	741	0.8044903808572544	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	491	0.5330698744951579	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	364	0.39518825726321277	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	314	0.3409041559907934	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	295	0.32027619750727404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207	0.22473617926781603	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	199	0.21605072306422896	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	189	0.20519390280974512	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.19867981065705476	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	168	0.18239458027532895	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG	161	0.17479480609719025	No Hit
GGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	146	0.15850957571546445	No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	144	0.15633821166456768	No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	137	0.14873843748642898	No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	135	0.1465670734355322	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGT	123	0.13353888913015155	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122	0.13245320710470318	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	110	0.11942502279932254	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	110	0.11942502279932254	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	104	0.1129109306466322	No Hit
GTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	103	0.11182524862118383	No Hit
GTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	102	0.11073956659573543	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99	0.10748252051939028	No Hit
TGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	97	0.10531115646849351	No Hit
GTTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	94	0.10205411039214835	No Hit
TTGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	93	0.10096842836669996	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010856820254483866	0.0	0.0	1.623094628045338	0.0
2	0.0010856820254483866	0.0	0.0	4.484952447127285	0.0
3	0.0010856820254483866	0.0	0.0	6.1232466235289005	0.0
4	0.0010856820254483866	0.0	0.0	7.933078559951362	0.0
5	0.0010856820254483866	0.0	0.0	11.193381682372866	0.0
6	0.0010856820254483866	0.0	0.0	13.311547314022668	0.0
7	0.0010856820254483866	0.0	0.0	15.405827941112607	0.0
8	0.0010856820254483866	0.0	0.0	18.221001433100273	0.0
9	0.0010856820254483866	0.0	0.0	19.56290441655448	0.0
10	0.0010856820254483866	0.0	0.0	22.45841837842533	0.0
11	0.0010856820254483866	0.0	0.0	29.53706518434881	0.0
12	0.0010856820254483866	0.0	0.0	34.79719459764624	0.0
13	0.0010856820254483866	0.0	0.0	36.69930950623181	0.0
14	0.0010856820254483866	0.0	0.0	37.47557215442741	0.0
15	0.0010856820254483866	0.0	0.0	38.556911451774006	0.0
16	0.0010856820254483866	0.0	0.0	40.84878620749555	0.0
17	0.0010856820254483866	0.0	0.0	43.55104876883658	0.0
18	0.0010856820254483866	0.0	0.0	45.979719459764624	0.0
19	0.0010856820254483866	0.0	0.0	47.36070699613497	0.0
20	0.0010856820254483866	0.0	0.0	48.495244712728535	0.0
21	0.0010856820254483866	0.0	0.0	49.66235289008555	0.0
22	0.0010856820254483866	0.0	0.0	50.473357363095495	0.0
23	0.0010856820254483866	0.0	0.0	51.125852260389976	0.0
24	0.0010856820254483866	0.0	0.0	51.490641420940634	0.0
25	0.0010856820254483866	0.0	0.0	51.745776696921006	0.0
26	0.0010856820254483866	0.0	0.0	51.94445650757806	0.0
27	0.0010856820254483866	0.0	0.0	52.10839449342077	0.0
28	0.0010856820254483866	0.0	0.0	52.246276110652715	0.0
29	0.0010856820254483866	0.0	0.0	52.39392886611369	0.0
30	0.0010856820254483866	0.0	0.0	52.528553437269295	0.0
31	0.0010856820254483866	0.0	0.0	52.64580709601772	0.0
32	0.0010856820254483866	0.0	0.0	52.76088939071525	0.0
33	0.0010856820254483866	0.0	0.0	52.8900855517436	0.0
34	0.0010856820254483866	0.0	0.0	53.01385330264472	0.0
35	0.0010856820254483866	0.0	0.0	53.13979241759674	0.0
36	0.0010856820254483866	0.0	0.0	53.240760845963436	0.0
37	0.0010856820254483866	0.0	0.0	53.346072002431924	0.0
38	0.0010856820254483866	0.0	0.0	53.45464020497677	0.0
39	0.0010856820254483866	0.0	0.0	53.57949363790333	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	35	1.1957309E-7	45.000004	14
AATCCTA	35	1.1957309E-7	45.000004	13
TCCTAGC	35	1.1957309E-7	45.000004	15
GGGTATG	35	1.1957309E-7	45.000004	7
GGGTAGA	35	1.1957309E-7	45.000004	7
TGGGCAT	35	1.1957309E-7	45.000004	6
TTCGGGC	35	1.1957309E-7	45.000004	4
ATGGGTA	20	6.9995027E-4	45.0	5
GCCCTAC	25	3.86283E-5	45.0	26
ACTAATC	40	6.697519E-9	45.0	10
GCCTTTG	20	6.9995027E-4	45.0	1
GGCATGG	20	6.9995027E-4	45.0	2
TATACTA	25	3.86283E-5	45.0	44
ACATGAT	20	6.9995027E-4	45.0	32
ATGATTT	20	6.9995027E-4	45.0	34
CACAATT	20	6.9995027E-4	45.0	43
GGGTACT	20	6.9995027E-4	45.0	7
GGACTAA	40	6.697519E-9	45.0	8
CAACTAG	20	6.9995027E-4	45.0	17
TATGCCA	20	6.9995027E-4	45.0	10
>>END_MODULE