FastQCFastQC Report
Sat 14 Jan 2017
SRR2933722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475940
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154343.2428457368575874No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC30920.6496617220658066No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC15110.3174769928982645No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT14670.3082321301004328Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC12880.2706223473547086No Hit
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC11270.23679455393537No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC10420.2189351598941043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.12879774761524562No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG5700.1197629953355465No Hit
TCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG5430.11409001134596798No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.11324956927343782No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4800.10085304870361811No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT4790.10064293818548556No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG207.029115E-445.0000049
CGGGCTT302.1628111E-645.0000046
ATCGCGG207.029115E-445.0000042
TCGTTCA253.8873484E-545.016
TTGCTCG502.1827873E-1145.01
AGACGTG253.8873484E-545.024
CAGACGT253.8873484E-545.023
CGTTTTT55700.043.9093361
GCCGATT650.041.538469
TACGGGA603.6379788E-1241.2500044
GGGACGT501.0786607E-940.57
CGGGATC1800.040.06
AGCTACG850.039.7058839
CGGGAAT1250.039.66
GTTTTTT64900.039.591682
CTAGGGA1650.039.5454524
CGAATAT750.039.014
AGGGATT1800.038.756
TTTGGGC10200.038.602944
CGCGGGT356.240578E-638.5714264
Produced by FastQC (version 0.11.5)