##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478942
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97078978247888	33.0	31.0	34.0	30.0	34.0
2	32.16558581206075	33.0	31.0	34.0	30.0	34.0
3	32.320990850666675	34.0	31.0	34.0	30.0	34.0
4	35.841193714479	37.0	35.0	37.0	35.0	37.0
5	35.916806210355325	37.0	35.0	37.0	35.0	37.0
6	35.89705851648007	37.0	35.0	37.0	35.0	37.0
7	35.8910389984591	37.0	35.0	37.0	35.0	37.0
8	35.756093639730906	37.0	35.0	37.0	35.0	37.0
9	37.554221596769544	39.0	37.0	39.0	35.0	39.0
10	37.09082310592932	39.0	37.0	39.0	33.0	39.0
11	37.02242025130391	39.0	37.0	39.0	33.0	39.0
12	37.106954495533905	39.0	37.0	39.0	34.0	39.0
13	37.09928133260395	39.0	37.0	39.0	33.0	39.0
14	38.12472491449904	40.0	38.0	41.0	33.0	41.0
15	38.156555491061546	40.0	37.0	41.0	33.0	41.0
16	38.31867741814249	40.0	38.0	41.0	34.0	41.0
17	38.37796643434904	40.0	38.0	41.0	34.0	41.0
18	38.267942673643155	39.0	38.0	41.0	34.0	41.0
19	38.12242192165231	39.0	37.0	41.0	34.0	41.0
20	37.734980436044445	39.0	35.0	41.0	34.0	41.0
21	37.666874068258785	39.0	35.0	41.0	33.0	41.0
22	37.68408700844779	39.0	35.0	41.0	34.0	41.0
23	37.56750295442872	39.0	35.0	41.0	33.0	41.0
24	37.4854053309169	39.0	35.0	41.0	33.0	41.0
25	37.41762050519687	39.0	35.0	41.0	33.0	41.0
26	37.40452914966739	39.0	35.0	41.0	33.0	41.0
27	37.3289083020491	39.0	35.0	41.0	33.0	41.0
28	37.31352021747936	39.0	35.0	41.0	33.0	41.0
29	37.17293534498958	39.0	35.0	41.0	33.0	41.0
30	36.97779063018069	39.0	35.0	41.0	32.0	41.0
31	36.751894801458214	39.0	35.0	40.0	31.0	41.0
32	36.5925727958709	39.0	35.0	41.0	31.0	41.0
33	36.21828321592176	39.0	35.0	41.0	30.0	41.0
34	36.013790813919016	39.0	35.0	41.0	29.0	41.0
35	35.845559587590984	39.0	35.0	41.0	27.0	41.0
36	35.54485094228529	39.0	35.0	40.0	25.0	41.0
37	35.39333155162838	38.0	35.0	40.0	25.0	41.0
38	35.38497772172831	38.0	35.0	40.0	25.0	41.0
39	35.427358636327575	39.0	35.0	40.0	25.0	41.0
40	35.33059117805496	39.0	35.0	40.0	24.0	41.0
41	35.13379490627258	38.0	35.0	40.0	23.0	41.0
42	34.7371602406972	38.0	34.0	40.0	22.0	41.0
43	34.575299305552655	38.0	34.0	40.0	22.0	41.0
44	34.60008518776804	38.0	34.0	40.0	22.0	41.0
45	34.60951221651056	38.0	34.0	40.0	22.0	41.0
46	34.354750679623	38.0	34.0	40.0	20.0	41.0
47	34.24384581013985	38.0	33.0	40.0	21.0	41.0
48	34.12341786688158	37.0	33.0	40.0	21.0	41.0
49	34.14340358540282	37.0	33.0	40.0	21.0	41.0
50	33.97762568327689	37.0	33.0	40.0	20.0	41.0
51	32.968891849117426	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	5.0
11	4.0
12	14.0
13	13.0
14	11.0
15	28.0
16	36.0
17	58.0
18	116.0
19	222.0
20	412.0
21	623.0
22	1050.0
23	1697.0
24	2920.0
25	5477.0
26	8385.0
27	9465.0
28	9278.0
29	9150.0
30	9929.0
31	11285.0
32	13792.0
33	17965.0
34	26565.0
35	35335.0
36	48434.0
37	62821.0
38	104100.0
39	99691.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.385382781213593	5.0106275916499285	47.48508170091577	25.118907926220714
2	30.57217784199339	4.927945346200584	47.69679835971788	16.803078452088144
3	19.102104221387975	4.768844661775329	56.88475848850174	19.244292628334954
4	15.650955648074296	5.093727424197502	57.91431947918537	21.34099744854283
5	14.569613857210268	6.070463646955164	57.116310534469726	22.24361196136484
6	18.44628368361931	7.121739166746704	60.278906422907156	14.153070726726828
7	71.07708240246208	2.7151513126850433	21.126148886504005	5.081617398348861
8	70.79416714341194	3.4419616571526404	19.81993644324365	5.943934756191773
9	64.06475105545138	7.243257012331347	22.488318000927045	6.203673931290219
10	28.764443293759996	30.152294014724124	27.557616579878147	13.525646111637734
11	18.29094128307812	19.853552204651084	40.20946168847167	21.646044823799123
12	17.57707613865562	17.369326557286684	44.034350714700324	21.019246589357376
13	21.158094299518524	18.864079575397437	43.95981141766644	16.0180147074176
14	17.59607635162504	21.18377590605961	42.87972238809709	18.340425354218258
15	13.758242125351295	20.891047350201067	44.788513014101916	20.56219751034572
16	14.836869600076835	21.02133452484852	41.27723189864326	22.864563976431384
17	15.217709033661697	20.672440504278182	39.87058975825883	24.239260703801296
18	15.808594777655749	19.520944080911676	43.06659261455458	21.603868526877992
19	16.23119292106351	22.239644883931668	42.412651218727945	19.116510976276878
20	16.115521294854073	25.59934188273319	39.7607643514246	18.524372470988137
21	15.579965841375365	25.919213599976615	39.94763457788208	18.55318598076594
22	14.00211299071704	23.943191451156924	38.81284163844474	23.241853919681297
23	12.979233393605071	24.893410893177045	37.310363259016746	24.816992454201134
24	14.202972384965193	24.587528343724294	37.56926725991874	23.640232011391777
25	14.282313933628707	26.36561420798343	35.19444943229034	24.15762242609752
26	14.262896133561057	26.981763971420342	36.11773450647468	22.637605388543918
27	15.019355162002915	26.227601672018743	38.37166086916579	20.381382296812557
28	13.57032793114824	24.475197414300688	37.79204997682392	24.162424677727156
29	15.811726680892468	22.983993886524882	36.4000233848775	24.80425604770515
30	20.692484684993172	25.109303422961442	34.280351274267026	19.917860617778352
31	19.93477289525663	30.063765549899568	29.55514446425663	20.44631709058717
32	18.757386071799925	29.432165063828187	28.57694668665517	23.233502177716716
33	19.979037127668903	26.35392176923302	29.620288051580356	24.04675305151772
34	20.824651001582655	24.52071440800765	30.169623879300627	24.48501071110907
35	23.000279783355815	24.2336232779752	28.648562874001442	24.117534064667538
36	23.2174250744349	29.289559069782978	29.523616638340343	17.96939921744178
37	20.330645464377735	32.482847609940244	28.77613573251041	18.410371193171613
38	19.302337234988787	28.157898033582356	29.69816804540007	22.84159668602879
39	20.75157325939258	22.006631283119876	33.822258227509806	23.419537229977745
40	26.940005261597438	20.88102525984357	32.77327943675852	19.40569004180047
41	28.123655891527577	23.001741338199615	29.048402520555726	19.826200249717086
42	24.619056169640583	24.483966743363496	32.797499488455806	18.099477598540116
43	19.836848720721925	24.392932755949573	36.176823080874094	19.59339544245441
44	19.538065151939065	23.433108810670188	32.707300675238336	24.32152536215241
45	23.330173590956736	21.30717289358628	30.543364332215596	24.819289183241395
46	25.473021785518913	22.22419416129719	32.93342408892935	19.369359964254546
47	20.706891439882074	25.482208701679955	34.98043604444797	18.830463813990004
48	19.242831073491153	24.381031523650044	36.29395626192733	20.08218114093147
49	22.472449691194342	19.968597450213178	37.165669329480394	20.393283529112086
50	21.868409953606076	18.705605271619525	33.90368771166446	25.52229706310994
51	20.05399401180101	18.252732063590162	32.23542725423955	29.45784667036927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	786.0
1	1353.5
2	1921.0
3	11774.5
4	21628.0
5	13507.0
6	5386.0
7	5173.0
8	4960.0
9	5176.0
10	5392.0
11	5307.5
12	5223.0
13	5134.5
14	5046.0
15	4648.0
16	4250.0
17	3923.5
18	3597.0
19	3479.5
20	3362.0
21	3189.0
22	3016.0
23	3033.0
24	3050.0
25	3157.5
26	3538.5
27	3812.0
28	4394.5
29	4977.0
30	5660.5
31	6344.0
32	6711.5
33	7079.0
34	8229.5
35	9380.0
36	10232.0
37	11084.0
38	12274.0
39	13464.0
40	16637.0
41	19810.0
42	23149.0
43	26488.0
44	30464.5
45	34441.0
46	41952.0
47	49463.0
48	56151.0
49	62839.0
50	59363.5
51	55888.0
52	45665.0
53	35442.0
54	27808.0
55	20174.0
56	16555.5
57	12937.0
58	11410.0
59	9883.0
60	8515.0
61	7147.0
62	6332.0
63	5517.0
64	4959.0
65	4401.0
66	3677.0
67	2953.0
68	2310.5
69	1668.0
70	1430.0
71	1192.0
72	1010.5
73	829.0
74	617.5
75	328.5
76	251.0
77	184.5
78	118.0
79	84.5
80	51.0
81	33.0
82	15.0
83	9.0
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.547414165821614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68485101839337	32.3792216189741
2	11.16495838533664	9.947400506715082
3	5.135949276804555	6.863797787053937
4	2.9365111149093073	5.23255907353614
5	1.9223383872365898	4.281760215154296
6	1.3499139410527716	3.6081105252176586
7	0.9350907309259138	2.915911185122428
8	0.6955866970620117	2.478927094580586
9	0.5442909699395395	2.1822079738152085
>10	2.487045140163756	17.975889361462034
>50	0.09249674435032114	2.84734322032164
>100	0.04530452784467793	3.473195314724889
>500	0.002359610825243642	0.56866977339936
>1k	0.0028315329902923706	2.0493134754517413
>5k	0.0	0.0
>10k+	4.7192216504872837E-4	3.1956928744708577
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15201	3.173870740089614	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	3117	0.6508094925899169	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	1573	0.32843225275711885	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	1452	0.3031682333142635	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1364	0.28479440099218695	No Hit
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1123	0.23447515565559085	No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1119	0.23363998145913284	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	577	0.12047387783907029	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	538	0.11233092942360454	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.11233092942360454	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG	536	0.11191334232537552	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	516	0.10773747134308538	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	484	0.10105607777142118	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.175870982290131E-4	0.0	0.0	0.32905863340446234	0.0
2	4.175870982290131E-4	0.0	0.0	1.1700790492376947	0.0
3	4.175870982290131E-4	0.0	0.0	1.722546780194679	0.0
4	4.175870982290131E-4	0.0	0.0	2.541643873370888	0.0
5	4.175870982290131E-4	0.0	0.0	4.236421111533338	0.0
6	4.175870982290131E-4	0.0	0.0	5.884637388243253	0.0
7	4.175870982290131E-4	0.0	0.0	7.550183529529671	0.0
8	4.175870982290131E-4	0.0	0.0	10.098091209373996	0.0
9	4.175870982290131E-4	0.0	0.0	11.533338065987113	0.0
10	4.175870982290131E-4	0.0	0.0	13.990002964868397	0.0
11	4.175870982290131E-4	0.0	0.0	19.378338086866467	0.0
12	4.175870982290131E-4	0.0	0.0	24.13757824538253	0.0
13	4.175870982290131E-4	0.0	0.0	25.7016507217993	0.0
14	4.175870982290131E-4	0.0	0.0	26.342646917580833	0.0
15	4.175870982290131E-4	0.0	0.0	27.1193589202868	0.0
16	4.175870982290131E-4	0.0	0.0	28.96154440412409	0.0
17	6.263806473435197E-4	0.0	0.0	31.505067419437008	0.0
18	6.263806473435197E-4	0.0	0.0	33.871324711551715	0.0
19	6.263806473435197E-4	0.0	0.0	35.31575848432587	0.0
20	6.263806473435197E-4	0.0	0.0	36.58584964358941	0.0
21	6.263806473435197E-4	0.0	0.0	38.046778106743616	0.0
22	6.263806473435197E-4	0.0	0.0	39.42794743413607	0.0
23	6.263806473435197E-4	0.0	0.0	40.53580600573765	0.0
24	8.351741964580262E-4	0.0	0.0	41.13420831749982	0.0
25	8.351741964580262E-4	0.0	0.0	41.55910318994784	0.0
26	8.351741964580262E-4	0.0	0.0	41.89024975884345	0.0
27	8.351741964580262E-4	0.0	0.0	42.189242121175425	0.0
28	8.351741964580262E-4	0.0	0.0	42.46902547698886	0.0
29	8.351741964580262E-4	0.0	0.0	42.74254502632887	0.0
30	0.0010439677455725328	0.0	0.0	43.02775701441928	0.0
31	0.0010439677455725328	0.0	0.0	43.263693724918674	0.0
32	0.0010439677455725328	0.0	0.0	43.47979504825219	0.0
33	0.0010439677455725328	0.0	0.0	43.677313745714514	0.0
34	0.0010439677455725328	0.0	0.0	43.88172263029762	0.0
35	0.0010439677455725328	0.0	0.0	44.10408776010456	0.0
36	0.0010439677455725328	0.0	0.0	44.30202404466512	0.0
37	0.0010439677455725328	0.0	0.0	44.48931185822083	0.0
38	0.0010439677455725328	0.0	0.0	44.7077099105946	0.0
39	0.0010439677455725328	0.0	0.0	45.09272521516175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCAT	25	3.887385E-5	45.000004	21
TAACCCG	30	2.1628402E-6	45.000004	37
TCTCGCG	20	7.029159E-4	45.000004	1
AATTCCG	30	2.1628402E-6	45.000004	11
CCTGGCG	20	7.029159E-4	45.000004	4
GTTCAAG	35	1.2099372E-7	45.0	10
CGTTTTT	5450	0.0	43.96789	1
ATGGGAC	125	0.0	43.2	5
GGATACT	50	1.0786607E-9	40.500004	8
TGGGCAA	100	0.0	40.500004	6
CGCAGGG	45	1.9243089E-8	40.000004	3
GTTTTTT	6440	0.0	39.444878	2
AGCGTTC	40	3.4525874E-7	39.375004	7
CGTTCAA	40	3.4525874E-7	39.375004	9
CTACGGG	40	3.4525874E-7	39.375004	3
AAAGCGT	40	3.4525874E-7	39.375004	5
TTTGGGA	3225	0.0	39.139534	4
CGTAGGG	75	0.0	39.0	3
CGAACGA	35	6.24066E-6	38.571426	22
TCCGATA	35	6.24066E-6	38.571426	14
>>END_MODULE