##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933720.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401466
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24590127183871	33.0	31.0	34.0	30.0	34.0
2	32.4450787862484	34.0	31.0	34.0	30.0	34.0
3	32.53442881838063	34.0	31.0	34.0	31.0	34.0
4	36.056331046713794	37.0	35.0	37.0	35.0	37.0
5	36.08690399685154	37.0	35.0	37.0	35.0	37.0
6	36.06487722497048	37.0	35.0	37.0	35.0	37.0
7	36.13666661684925	37.0	35.0	37.0	35.0	37.0
8	35.99805712065281	37.0	35.0	37.0	35.0	37.0
9	37.830137545894296	39.0	38.0	39.0	35.0	39.0
10	37.29728793970099	39.0	37.0	39.0	34.0	39.0
11	37.20258751675111	39.0	37.0	39.0	34.0	39.0
12	37.28374756517364	39.0	37.0	39.0	34.0	39.0
13	37.286196091325294	39.0	37.0	39.0	34.0	39.0
14	38.39193356348981	40.0	38.0	41.0	34.0	41.0
15	38.46097054296005	40.0	38.0	41.0	34.0	41.0
16	38.525979784091305	40.0	38.0	41.0	34.0	41.0
17	38.518596842571974	40.0	38.0	41.0	34.0	41.0
18	38.252195702749425	39.0	38.0	41.0	34.0	41.0
19	37.93485874270797	39.0	37.0	41.0	34.0	41.0
20	37.51895303711896	39.0	35.0	41.0	34.0	41.0
21	37.40272899822152	39.0	35.0	41.0	33.0	41.0
22	37.44269502274165	39.0	35.0	41.0	33.0	41.0
23	37.40459715143997	39.0	35.0	41.0	33.0	41.0
24	37.313884612893744	39.0	35.0	41.0	33.0	41.0
25	37.23005684167526	39.0	35.0	41.0	33.0	41.0
26	37.15062296682658	39.0	35.0	40.0	33.0	41.0
27	37.08550661824413	39.0	35.0	40.0	33.0	41.0
28	36.97702420628397	39.0	35.0	40.0	33.0	41.0
29	36.8828792475577	39.0	35.0	40.0	33.0	41.0
30	36.76829669262154	39.0	35.0	40.0	32.0	41.0
31	36.40973332735524	38.0	35.0	40.0	31.0	41.0
32	35.89125106484733	38.0	35.0	40.0	30.0	41.0
33	35.43349374542302	38.0	35.0	40.0	25.0	41.0
34	34.97972680127333	38.0	35.0	40.0	21.0	41.0
35	34.52742199837595	38.0	34.0	40.0	18.0	41.0
36	34.36589898023743	38.0	34.0	40.0	18.0	41.0
37	34.2615364688417	38.0	34.0	40.0	17.0	41.0
38	34.11622403889744	38.0	34.0	40.0	15.0	41.0
39	34.109095166215816	38.0	34.0	40.0	15.0	41.0
40	33.955258975853496	38.0	34.0	40.0	15.0	41.0
41	33.63600404517444	38.0	33.0	40.0	15.0	41.0
42	33.501828299283126	38.0	33.0	40.0	13.0	41.0
43	33.51849222599174	38.0	33.0	40.0	12.0	41.0
44	33.43062675295044	38.0	33.0	40.0	11.0	41.0
45	33.46379020888444	38.0	33.0	40.0	12.0	41.0
46	33.25138866055905	38.0	33.0	40.0	10.0	41.0
47	33.06880034672924	37.0	33.0	40.0	10.0	41.0
48	33.000463301998174	37.0	33.0	40.0	10.0	41.0
49	33.027133057344834	37.0	33.0	40.0	10.0	41.0
50	32.935105338932814	37.0	32.0	40.0	10.0	41.0
51	31.971576671498955	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	1.0
12	3.0
13	6.0
14	6.0
15	16.0
16	27.0
17	59.0
18	118.0
19	243.0
20	419.0
21	677.0
22	1137.0
23	2084.0
24	3728.0
25	7177.0
26	11195.0
27	11696.0
28	10427.0
29	8970.0
30	8670.0
31	9281.0
32	10834.0
33	14102.0
34	20778.0
35	28367.0
36	38169.0
37	48200.0
38	82365.0
39	82690.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.585304857696542	4.289528876667015	45.1978498801891	29.927316385447334
2	35.799046494597306	4.009305893898861	44.06475268142259	16.12689493008125
3	17.540713285807517	4.183168686763014	61.13743131423334	17.138686713196137
4	13.884114719552839	4.314437586246407	61.885688949998254	19.915758744202495
5	13.085541490437546	5.272426556669805	61.78480867620172	19.857223276690927
6	17.119756093915797	5.948698021750285	63.1894108093836	13.742135074950307
7	67.37830849934988	1.649703835443101	26.647088420937266	4.324899244269751
8	67.89815326827178	1.996682159884025	25.180712687998486	4.924451883845705
9	62.52434826361386	4.976511086866633	27.45263608873479	5.046504560784724
10	26.731528946411405	28.24548031464682	32.49092077535831	12.532069963583467
11	18.12432435125266	20.544703661082135	42.671608554647214	18.65936343301799
12	16.56404278319957	19.320689672350834	45.586674836723404	18.528592707726183
13	18.96349877698236	19.857721450882515	46.84755371563221	14.331226056502915
14	16.57724439927665	22.174729615957517	45.393632337483126	15.854393647282707
15	13.679116039714447	21.11884941688711	47.236627759262305	17.96540678413614
16	14.640343142383166	21.705200440385987	44.69793207893072	18.956524338300127
17	14.90138641877519	21.211509816522444	42.956315105139666	20.9307886595627
18	16.186924920167588	19.30126087887891	45.65716648483309	18.854647716120418
19	15.450872552096568	22.310232996069406	44.8090249236548	17.429869528179225
20	16.317446558363596	24.98816836294979	42.51343824881808	16.18094682986853
21	15.52684411631371	25.565552251000085	43.36506702933748	15.542536603348728
22	14.285144943781042	23.017889435219917	42.30146513029746	20.39550049070158
23	13.402380276287406	24.780678812153457	40.75064887188454	21.066292039674593
24	13.609620739987943	24.467825419836302	41.75845526146673	20.16409857870903
25	13.718222713754091	26.973641603523085	38.60999437063163	20.698141312091188
26	13.743131423333482	27.350759466555076	39.8885584333418	19.01755067676964
27	14.8886829768897	25.55010885106086	41.3379962437666	18.223211928282844
28	12.437665954277573	25.06264540459217	40.960380206543014	21.539308434587237
29	14.648064842352776	24.63496286111402	39.6389731633563	21.077999133176906
30	20.355148381182964	24.23766894332272	37.83583167690415	17.57135099859017
31	19.184439030951562	28.015822012324833	34.205387255707834	18.594351701015775
32	16.996457981497812	28.89211041532782	33.91868800844903	20.192743594725332
33	20.677217996044497	25.37425336143036	32.80053603543015	21.147992607095
34	19.12391086667364	25.236757284552112	33.2117788305859	22.42755301818834
35	18.59136265586625	28.97331280855664	32.36687540165294	20.06844913392417
36	22.68784903329298	28.371518385118538	31.431304269850997	17.509328311737484
37	20.897161901630522	27.449647043585262	31.757110191149433	19.89608086363478
38	18.992392880094453	28.922000866823094	32.130491747744514	19.95511450533794
39	22.85623191004967	26.165852151863422	32.077436196340415	18.900479741746498
40	26.004941887980554	24.511913835791823	32.49266438502887	16.990479891198756
41	21.646415885778623	25.13836788171352	34.00686484036008	19.20835139214778
42	20.282664036306937	24.74829748970025	37.243751650202015	17.725286823790807
43	19.09078228293305	24.565467561387514	37.1662855634101	19.177464592269335
44	19.98973761165329	25.52320744471512	32.08167067696891	22.405384266662683
45	22.925976296871966	23.804506483737104	30.812821010994707	22.456696208396227
46	23.867525518972965	23.92581189938874	33.206548001574234	19.000114580064064
47	18.419990733960038	26.49937977313147	36.75828090049967	18.322348592408822
48	17.673476707865674	25.414854558044762	37.74441671274778	19.16725202134178
49	21.528348602372304	22.00136499728495	37.14312046350127	19.327165936841475
50	19.86071049603204	21.745303462808806	35.077939352273916	23.316046688885237
51	18.647656339515674	20.825175730946082	33.22772040471671	27.29944752482153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	572.0
1	1047.5
2	1523.0
3	13230.0
4	24937.0
5	15584.0
6	6231.0
7	6181.0
8	6131.0
9	6387.0
10	6643.0
11	6586.5
12	6530.0
13	6348.5
14	6167.0
15	5863.0
16	5559.0
17	5177.5
18	4796.0
19	4491.5
20	4187.0
21	4004.0
22	3821.0
23	3662.5
24	3504.0
25	3673.5
26	4044.0
27	4245.0
28	4422.5
29	4600.0
30	5109.5
31	5619.0
32	6444.5
33	7270.0
34	8005.5
35	8741.0
36	9531.5
37	10322.0
38	11354.0
39	12386.0
40	14442.5
41	16499.0
42	18904.5
43	21310.0
44	24291.0
45	27272.0
46	32204.5
47	37137.0
48	42468.0
49	47799.0
50	44638.0
51	41477.0
52	33961.0
53	26445.0
54	21101.0
55	15757.0
56	12845.0
57	9933.0
58	8351.0
59	6769.0
60	5907.5
61	5046.0
62	4044.0
63	3042.0
64	2559.5
65	2077.0
66	1626.5
67	1176.0
68	894.0
69	612.0
70	522.0
71	432.0
72	377.0
73	322.0
74	324.0
75	289.0
76	252.0
77	176.5
78	101.0
79	72.0
80	43.0
81	23.5
82	4.0
83	2.5
84	1.0
85	1.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.857675319737076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55012641406097	36.15653420289222
2	10.485611541367245	10.036339853512828
3	4.597583668484922	6.600889993850312
4	2.655840030299449	5.0840931948492685
5	1.6057512760992094	3.8423761607904727
6	1.140800983163906	3.2757649834017037
7	0.7944639478466705	2.661483836849774
8	0.6058467318868856	2.3195532950533093
9	0.4256785375991749	1.8334786718701621
>10	2.0148624613538573	15.741069119089499
>50	0.07916468350776952	2.583003466673899
>100	0.04010316204011803	3.423482834581166
>500	0.002083281144941197	0.7235799540436202
>1k	0.0015624608587058975	1.392573614619947
>5k	0.0	0.0
>10k+	5.208202862352992E-4	4.325776817921815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17355	4.3229065475034005	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCC	2908	0.7243452745687057	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	1438	0.35818724375165023	No Hit
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	1241	0.3091170858802489	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	972	0.24211265711168564	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	776	0.19329158633607826	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	589	0.14671229942261613	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	566	0.14098329621935607	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	432	0.10760562538297141	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	403	0.10038209960494787	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4505985562911928	0.0
2	0.0	0.0	0.0	1.3669899817170073	0.0
3	0.0	0.0	0.0	1.9767551922205118	0.0
4	0.0	0.0	0.0	2.8089551792679828	0.0
5	0.0	0.0	0.0	4.4591571889026715	0.0
6	0.0	0.0	0.0	5.938236363726941	0.0
7	0.0	0.0	0.0	7.324904226011667	0.0
8	0.0	0.0	0.0	9.478760343341653	0.0
9	0.0	0.0	0.0	10.51620809732331	0.0
10	2.490870957939153E-4	0.0	0.0	12.526590047476	0.0
11	2.490870957939153E-4	0.0	0.0	16.91824463341852	0.0
12	2.490870957939153E-4	0.0	0.0	20.56039614811715	0.0
13	2.490870957939153E-4	0.0	0.0	21.845934649509548	0.0
14	2.490870957939153E-4	0.0	0.0	22.298774989662885	0.0
15	2.490870957939153E-4	0.0	0.0	22.860964564869754	0.0
16	2.490870957939153E-4	0.0	0.0	24.274035659308634	0.0
17	2.490870957939153E-4	0.0	0.0	26.19648986464607	0.0
18	4.981741915878306E-4	0.0	0.0	28.054928686364473	0.0
19	4.981741915878306E-4	0.0	0.0	29.214678204380945	0.0
20	4.981741915878306E-4	0.0	0.0	30.202059452108024	0.0
21	4.981741915878306E-4	0.0	0.0	31.356079966921232	0.0
22	4.981741915878306E-4	0.0	0.0	32.41121290470426	0.0
23	4.981741915878306E-4	0.0	0.0	33.221991401513456	0.0
24	4.981741915878306E-4	0.0	0.0	33.718172896334934	0.0
25	4.981741915878306E-4	0.0	0.0	34.06166400143474	0.0
26	4.981741915878306E-4	0.0	0.0	34.34412876806504	0.0
27	4.981741915878306E-4	0.0	0.0	34.609157437989765	0.0
28	7.472612873817459E-4	0.0	0.0	34.85774635959209	0.0
29	7.472612873817459E-4	0.0	0.0	35.114555155355625	0.0
30	7.472612873817459E-4	0.0	0.0	35.38008199947193	0.0
31	7.472612873817459E-4	0.0	0.0	35.607249430835985	0.0
32	7.472612873817459E-4	0.0	0.0	35.8687908814196	0.0
33	7.472612873817459E-4	0.0	0.0	36.06556968709679	0.0
34	7.472612873817459E-4	0.0	0.0	36.27031927983939	0.0
35	7.472612873817459E-4	0.0	0.0	36.484534182222156	0.0
36	7.472612873817459E-4	0.0	0.0	36.684052945953084	0.0
37	7.472612873817459E-4	0.0	0.0	36.88880253869568	0.0
38	7.472612873817459E-4	0.0	0.0	37.11173548943123	0.0
39	7.472612873817459E-4	0.0	0.0	37.50155679434871	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	25	3.886255E-5	45.000004	1
GCGATTC	50	2.1827873E-11	45.000004	9
TAGGGCG	30	2.1619599E-6	45.000004	5
ATTGCGG	30	2.1619599E-6	45.000004	2
GTGTTCG	20	7.027796E-4	45.0	1
TAGCGCG	20	7.027796E-4	45.0	1
TGACGTA	20	7.027796E-4	45.0	25
CTACGAA	20	7.027796E-4	45.0	11
TTGCGGC	40	6.7957444E-9	45.0	3
GCTACGA	20	7.027796E-4	45.0	10
AGCTACG	20	7.027796E-4	45.0	9
CAGACCG	20	7.027796E-4	45.0	42
CGTTTTT	7335	0.0	44.47853	1
AACACGT	120	0.0	43.125004	41
AGCGGGA	60	3.6379788E-12	41.250004	4
GCCGATT	50	1.0786607E-9	40.500004	9
GTTTTTT	8345	0.0	40.22768	2
GACACGA	140	0.0	40.178574	25
CACGACC	140	0.0	40.178574	27
TTGGGAC	1240	0.0	39.737904	5
>>END_MODULE