FastQCFastQC Report
Sat 14 Jan 2017
SRR2933719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403010
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170274.224957197091884No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCC28590.7094116771295997No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC15170.3764174586238555No Hit
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT11830.2935411031984318TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC9780.24267387906007293TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC8200.20346889655343542TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC6170.1530979380164264TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG5550.13771370437458128No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.11662241631721297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG351.2093005E-745.0000049
GTGTTCG207.027829E-445.01
GCAACGA253.8862825E-545.09
CTACGGG207.027829E-445.03
ATAGTAC207.027829E-445.010
CAATCGG253.8862825E-545.020
AGTACGG207.027829E-445.02
ACGACGC253.8862825E-545.012
CGCGCAA302.1619799E-644.99999616
TATTGCG302.1619799E-644.9999961
GTCTTCG302.1619799E-644.9999961
AAGCTAC302.1619799E-644.9999968
ACGCGCA302.1619799E-644.99999615
CGTTTTT71250.044.6526341
CGCTTGG900.042.5000042
GCGTTTG556.002665E-1140.9090921
TCTTTCG556.002665E-1140.9090921
GCGATGC950.040.2631579
GTTTGCG900.040.0000041
TTGTGCG451.9230356E-840.0000041
Produced by FastQC (version 0.11.5)