##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933719.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403010
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1283814297412	33.0	31.0	34.0	30.0	34.0
2	32.36122676856654	34.0	31.0	34.0	30.0	34.0
3	32.47324880275924	34.0	31.0	34.0	31.0	34.0
4	35.96604054489963	37.0	35.0	37.0	35.0	37.0
5	36.05562889258331	37.0	35.0	37.0	35.0	37.0
6	36.058152403166176	37.0	35.0	37.0	35.0	37.0
7	36.0629761048113	37.0	35.0	37.0	35.0	37.0
8	35.960844644053495	37.0	35.0	37.0	35.0	37.0
9	37.80534974318255	39.0	38.0	39.0	35.0	39.0
10	37.316019453611574	39.0	37.0	39.0	34.0	39.0
11	37.21085829135753	39.0	37.0	39.0	34.0	39.0
12	37.28103272871641	39.0	37.0	39.0	34.0	39.0
13	37.29347410734225	39.0	37.0	39.0	34.0	39.0
14	38.4314359445175	40.0	38.0	41.0	34.0	41.0
15	38.47432321778616	40.0	38.0	41.0	34.0	41.0
16	38.604858440237216	40.0	38.0	41.0	34.0	41.0
17	38.629897521153325	40.0	38.0	41.0	34.0	41.0
18	38.41067219175703	39.0	38.0	41.0	35.0	41.0
19	38.1414332150567	39.0	37.0	41.0	34.0	41.0
20	37.678618396565845	39.0	35.0	41.0	34.0	41.0
21	37.613828440981614	39.0	35.0	41.0	34.0	41.0
22	37.62748318900275	39.0	35.0	41.0	34.0	41.0
23	37.51201955286469	39.0	35.0	41.0	34.0	41.0
24	37.43811816083968	39.0	35.0	41.0	33.0	41.0
25	37.37323887744721	39.0	35.0	41.0	33.0	41.0
26	37.338559837224885	39.0	35.0	41.0	33.0	41.0
27	37.28133048807722	39.0	35.0	41.0	33.0	41.0
28	37.236932086052455	39.0	35.0	41.0	33.0	41.0
29	37.07984913525719	39.0	35.0	41.0	33.0	41.0
30	36.87972506885685	39.0	35.0	41.0	33.0	41.0
31	36.53429691570929	39.0	35.0	41.0	31.0	41.0
32	36.10876653184784	39.0	35.0	41.0	30.0	41.0
33	35.57534304359693	39.0	35.0	41.0	25.0	41.0
34	35.1413612565445	39.0	35.0	41.0	22.0	41.0
35	34.741579613409094	38.0	35.0	41.0	19.0	41.0
36	34.55508051909382	38.0	35.0	41.0	18.0	41.0
37	34.47392868663309	38.0	35.0	41.0	18.0	41.0
38	34.31990521327014	38.0	34.0	40.0	17.0	41.0
39	34.348686136820426	38.0	34.0	40.0	17.0	41.0
40	34.181648098062084	38.0	34.0	40.0	15.0	41.0
41	33.89060321083844	38.0	33.0	40.0	15.0	41.0
42	33.679794546041045	38.0	33.0	40.0	15.0	41.0
43	33.733130691546116	38.0	33.0	40.0	15.0	41.0
44	33.660266494627926	38.0	33.0	40.0	12.0	41.0
45	33.660832237413466	38.0	33.0	40.0	12.0	41.0
46	33.418361827249946	38.0	33.0	40.0	12.0	41.0
47	33.33673109848391	37.0	33.0	40.0	12.0	41.0
48	33.225230143172624	37.0	33.0	40.0	10.0	41.0
49	33.23655492419543	37.0	33.0	40.0	10.0	41.0
50	33.05546016227885	37.0	33.0	40.0	10.0	41.0
51	32.2248902012357	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	6.0
13	7.0
14	9.0
15	20.0
16	22.0
17	44.0
18	103.0
19	246.0
20	410.0
21	650.0
22	1136.0
23	1995.0
24	3677.0
25	7100.0
26	10674.0
27	11639.0
28	10131.0
29	8777.0
30	8511.0
31	9054.0
32	10496.0
33	13564.0
34	20158.0
35	27108.0
36	36835.0
37	45954.0
38	81553.0
39	93072.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.73000669958562	4.47135306816208	45.187712463710575	29.61092776854172
2	35.815984715019475	4.053745564626188	44.017021910126296	16.113247810228035
3	17.80923550284112	4.301630232500434	60.74514280042679	17.143991464231657
4	13.976079998014937	4.397409493560954	61.493263194461676	20.133247313962432
5	13.175851715838316	5.232872633433414	61.703431676633336	19.887843974094935
6	17.14969851864718	6.00059551872162	63.099923078831786	13.74978288379941
7	67.61370685590929	1.672166943748294	26.395374804595416	4.318751395747004
8	68.19930026550209	2.006898091858763	24.930150616610007	4.863651026029131
9	62.84831641894743	4.989950621572666	27.10776407533312	5.053968884146796
10	26.771792263219275	28.439740949356096	32.19622341877373	12.592243368650902
11	18.22560234237364	20.639934492940622	42.506637552418056	18.627825612267685
12	16.717947445472817	19.41763231681596	45.22939877422397	18.63502146348726
13	19.013423984516514	19.96774273591226	46.5261407905511	14.492692489020124
14	16.754174834371355	22.24833130691546	45.154710801220816	15.84278305749237
15	13.725465869333267	21.20791047368552	47.11347112974864	17.953152527232575
16	14.748765539316642	21.910622565196896	44.29393811567951	19.04667377980695
17	15.054961415349494	21.253318776209028	42.79695292920771	20.894766879233767
18	16.269571474653237	19.33351529738716	45.512022034192704	18.884891193766904
19	15.612019552864693	22.42425746259398	44.577057641249596	17.386665343291728
20	16.410759038237266	25.0519838217414	42.52276618446192	16.014490955559417
21	15.635840301729488	25.606064365648496	42.93739609438972	15.820699238232303
22	14.345797871020572	23.168159599017393	42.225503089253365	20.260539440708666
23	13.564179548894566	25.008560581623286	40.32083571127267	21.106424158209474
24	13.704622714076573	24.724696657651172	41.52452792734672	20.046152700925536
25	13.759956328627082	27.185925907545716	38.41269447408253	20.64142328974467
26	13.949033522741372	27.53231929728791	39.48239497779211	19.036252202178606
27	15.020967221657031	25.710280141931964	41.01957767797325	18.249174958437756
28	12.678345450485098	25.147266817200563	40.73496935559911	21.43941837671522
29	14.78300786580978	24.69417632316816	39.3657725614749	21.157043249547158
30	20.30718840723555	24.45720949852361	37.69037989131783	17.545222202923007
31	19.205726905039576	27.900548373489492	34.21081362745341	18.68291109401752
32	17.043497679958314	28.988362571648345	33.74730155579266	20.22083819260068
33	20.65308553137639	25.281010396764348	32.93615543038634	21.12974864147292
34	19.291084588471747	25.43733406118955	33.00464008337262	22.266941266966082
35	18.49433016550458	29.117391627999307	32.211111386814224	20.177166819681894
36	22.774372844346296	28.490608173494454	31.336939530036474	17.398079452122776
37	20.900473933649288	27.49013672117317	31.759013423984516	19.850375921193024
38	18.805984963152277	28.883402396962854	32.13369395300365	20.17691868688122
39	22.9138234783256	26.157167315947493	32.10416614972333	18.824843056003573
40	26.055681000471452	24.513287511476143	32.35552467680703	17.07550681124538
41	21.445621696732093	25.479764770104964	34.04282772139649	19.031785811766458
42	20.279645666360636	24.900622813329694	37.18865536835314	17.631076151956528
43	19.088111957519665	24.64380536463115	37.12091511376889	19.147167564080295
44	19.85781990521327	25.740552343614304	32.14560432743605	22.256023423736384
45	23.01406912979827	24.000645145281755	30.646137812957498	22.33914791196248
46	23.91627999305228	23.999900746879728	33.25227661844619	18.831542641621795
47	18.342472891491525	26.555916726632095	36.875759906702065	18.225850475174312
48	17.61792511352076	25.741296742016328	37.61246619190591	19.028311952557008
49	21.40939430783355	22.104414282524008	37.25813255254212	19.22805885710032
50	19.89007716930101	22.05925411280117	34.76117217935039	23.28949653854743
51	18.56207042008883	20.994019999503735	33.20463512071661	27.239274459690826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	472.0
1	1037.5
2	1603.0
3	13075.5
4	24548.0
5	15521.5
6	6495.0
7	6317.0
8	6139.0
9	6351.5
10	6564.0
11	6613.0
12	6662.0
13	6449.5
14	6237.0
15	5776.5
16	5316.0
17	4973.0
18	4630.0
19	4411.0
20	4192.0
21	3889.0
22	3586.0
23	3628.0
24	3670.0
25	3716.5
26	4077.5
27	4392.0
28	4590.0
29	4788.0
30	5324.5
31	5861.0
32	6559.0
33	7257.0
34	8029.5
35	8802.0
36	9717.5
37	10633.0
38	11718.0
39	12803.0
40	14814.5
41	16826.0
42	18929.0
43	21032.0
44	24143.0
45	27254.0
46	32343.0
47	37432.0
48	42557.5
49	47683.0
50	44651.0
51	41619.0
52	34091.0
53	26563.0
54	21030.0
55	15497.0
56	12863.5
57	10230.0
58	8533.5
59	6837.0
60	5975.0
61	5113.0
62	4104.5
63	3096.0
64	2540.0
65	1984.0
66	1617.0
67	1250.0
68	944.0
69	638.0
70	528.5
71	419.0
72	386.0
73	353.0
74	350.5
75	309.5
76	271.0
77	179.5
78	88.0
79	70.0
80	52.0
81	27.5
82	3.0
83	4.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	403010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.80526672276712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38076270038849	36.03597466657686
2	10.566081278190135	10.10228667436631
3	4.6255508317890595	6.6337707376018
4	2.6614587906991347	5.089269894441015
5	1.714169695613151	4.09731697534356
6	1.1190169837886006	3.209694322639225
7	0.7616977696328923	2.548921552760607
8	0.6025495348804554	2.3044032982911555
9	0.4235667122917306	1.8223847690392572
>10	2.020877682155091	15.869282289105044
>50	0.0819751228138386	2.6638145440696324
>100	0.038115821435732576	3.2439030935839606
>500	0.002088538160862059	0.7413351945171102
>1k	0.0015664036206465442	1.3875698135759649
>5k	0.0	0.0
>10k+	5.221345402155147E-4	4.250072174088496
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17027	4.224957197091884	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCC	2859	0.7094116771295997	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	1517	0.3764174586238555	No Hit
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	1183	0.2935411031984318	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	978	0.24267387906007293	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	820	0.20346889655343542	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	617	0.1530979380164264	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	555	0.13771370437458128	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.11662241631721297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.481328006749212E-4	0.0	0.0	0.4342324011811121	0.0
2	2.481328006749212E-4	0.0	0.0	1.3282548820128532	0.0
3	2.481328006749212E-4	0.0	0.0	1.908637502791494	0.0
4	2.481328006749212E-4	0.0	0.0	2.7349197290389817	0.0
5	2.481328006749212E-4	0.0	0.0	4.358452643854991	0.0
6	2.481328006749212E-4	0.0	0.0	5.807796332597206	0.0
7	2.481328006749212E-4	0.0	0.0	7.191384829160567	0.0
8	2.481328006749212E-4	0.0	0.0	9.266271308404258	0.0
9	2.481328006749212E-4	0.0	0.0	10.295278032803155	0.0
10	4.962656013498424E-4	0.0	0.0	12.28108483660455	0.0
11	4.962656013498424E-4	0.0	0.0	16.601573161956278	0.0
12	4.962656013498424E-4	0.0	0.0	20.266742760725542	0.0
13	4.962656013498424E-4	0.0	0.0	21.481104687228605	0.0
14	4.962656013498424E-4	0.0	0.0	21.937917173271135	0.0
15	4.962656013498424E-4	0.0	0.0	22.520532989255848	0.0
16	4.962656013498424E-4	0.0	0.0	23.851517332076128	0.0
17	4.962656013498424E-4	0.0	0.0	25.802337410982357	0.0
18	4.962656013498424E-4	0.0	0.0	27.593856231855288	0.0
19	4.962656013498424E-4	0.0	0.0	28.689114414034393	0.0
20	4.962656013498424E-4	0.0	0.0	29.648395821443636	0.0
21	4.962656013498424E-4	0.0	0.0	30.821071437433314	0.0
22	4.962656013498424E-4	0.0	0.0	31.846108037021413	0.0
23	4.962656013498424E-4	0.0	0.0	32.65129897521153	0.0
24	7.443984020247637E-4	0.0	0.0	33.12250316369321	0.0
25	7.443984020247637E-4	0.0	0.0	33.4688965534354	0.0
26	7.443984020247637E-4	0.0	0.0	33.74779782139401	0.0
27	7.443984020247637E-4	0.0	0.0	33.99593062206893	0.0
28	7.443984020247637E-4	0.0	0.0	34.235130641919554	0.0
29	7.443984020247637E-4	0.0	0.0	34.476563856976256	0.0
30	7.443984020247637E-4	0.0	0.0	34.751743132924744	0.0
31	7.443984020247637E-4	0.0	0.0	34.96538547430585	0.0
32	7.443984020247637E-4	0.0	0.0	35.21500707178482	0.0
33	7.443984020247637E-4	0.0	0.0	35.4269124835612	0.0
34	9.925312026996848E-4	0.0	0.0	35.62790005210789	0.0
35	9.925312026996848E-4	0.0	0.0	35.84600878390114	0.0
36	9.925312026996848E-4	0.0	0.0	36.03210838440734	0.0
37	9.925312026996848E-4	0.0	0.0	36.24624699138979	0.0
38	0.0012406640033746062	0.0	0.0	36.48519887843974	0.0
39	0.0012406640033746062	0.0	0.0	36.889903476340535	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTACG	35	1.2093005E-7	45.000004	9
GTGTTCG	20	7.027829E-4	45.0	1
GCAACGA	25	3.8862825E-5	45.0	9
CTACGGG	20	7.027829E-4	45.0	3
ATAGTAC	20	7.027829E-4	45.0	10
CAATCGG	25	3.8862825E-5	45.0	20
AGTACGG	20	7.027829E-4	45.0	2
ACGACGC	25	3.8862825E-5	45.0	12
CGCGCAA	30	2.1619799E-6	44.999996	16
TATTGCG	30	2.1619799E-6	44.999996	1
GTCTTCG	30	2.1619799E-6	44.999996	1
AAGCTAC	30	2.1619799E-6	44.999996	8
ACGCGCA	30	2.1619799E-6	44.999996	15
CGTTTTT	7125	0.0	44.652634	1
CGCTTGG	90	0.0	42.500004	2
GCGTTTG	55	6.002665E-11	40.909092	1
TCTTTCG	55	6.002665E-11	40.909092	1
GCGATGC	95	0.0	40.263157	9
GTTTGCG	90	0.0	40.000004	1
TTGTGCG	45	1.9230356E-8	40.000004	1
>>END_MODULE