##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933718.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127863
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02739651032746	33.0	31.0	34.0	30.0	34.0
2	32.235118838131434	34.0	31.0	34.0	30.0	34.0
3	32.342280409500795	34.0	31.0	34.0	30.0	34.0
4	35.93745649640631	37.0	35.0	37.0	35.0	37.0
5	35.94645049779842	37.0	35.0	37.0	35.0	37.0
6	35.903998811227645	37.0	35.0	37.0	35.0	37.0
7	35.96005881294823	37.0	35.0	37.0	35.0	37.0
8	35.79188662865724	37.0	35.0	37.0	35.0	37.0
9	37.59681846976842	39.0	37.0	39.0	35.0	39.0
10	37.06885494630972	39.0	37.0	39.0	33.0	39.0
11	37.071998936361574	39.0	37.0	39.0	33.0	39.0
12	37.12428927836825	39.0	37.0	39.0	34.0	39.0
13	37.04943572417353	39.0	37.0	39.0	33.0	39.0
14	37.968231622908895	40.0	37.0	41.0	33.0	41.0
15	38.05760071326341	40.0	37.0	41.0	33.0	41.0
16	38.172989840688864	40.0	37.0	41.0	33.0	41.0
17	38.18748973510711	40.0	37.0	41.0	33.0	41.0
18	37.95726676208129	39.0	37.0	40.0	33.0	41.0
19	37.679516357351226	39.0	37.0	40.0	34.0	41.0
20	37.23062965830616	39.0	35.0	40.0	33.0	41.0
21	37.10108475477659	39.0	35.0	40.0	33.0	41.0
22	37.12875499558121	39.0	35.0	40.0	33.0	41.0
23	37.097260348967254	38.0	35.0	40.0	33.0	41.0
24	36.978171949664876	38.0	35.0	40.0	33.0	41.0
25	36.878534055981795	38.0	35.0	40.0	32.0	41.0
26	36.80840430773562	38.0	35.0	40.0	32.0	41.0
27	36.738313663843336	38.0	35.0	40.0	32.0	41.0
28	36.61043460578901	38.0	35.0	40.0	32.0	41.0
29	36.508161078654496	38.0	35.0	40.0	32.0	41.0
30	36.39728459366666	38.0	35.0	40.0	31.0	41.0
31	36.08453579221511	38.0	35.0	40.0	30.0	41.0
32	35.72233562484847	38.0	35.0	40.0	30.0	41.0
33	35.37079530434919	38.0	35.0	40.0	27.0	41.0
34	35.03901832430023	38.0	35.0	40.0	24.0	41.0
35	34.73754721850731	38.0	34.0	40.0	23.0	41.0
36	34.603575702118675	38.0	34.0	40.0	21.0	41.0
37	34.47096501724502	38.0	34.0	40.0	21.0	41.0
38	34.372461149824424	38.0	34.0	40.0	20.0	41.0
39	34.34121677107529	38.0	34.0	40.0	20.0	41.0
40	34.15694141385702	38.0	34.0	40.0	18.0	41.0
41	33.99904585376536	38.0	33.0	40.0	18.0	41.0
42	33.93195842425096	38.0	33.0	40.0	18.0	41.0
43	33.84316807833384	38.0	33.0	40.0	17.0	41.0
44	33.692170526266395	37.0	33.0	40.0	16.0	41.0
45	33.73358985789478	37.0	33.0	40.0	17.0	41.0
46	33.48608276045455	37.0	33.0	40.0	16.0	41.0
47	33.303950321828836	37.0	33.0	40.0	15.0	41.0
48	33.28007320335046	37.0	33.0	40.0	15.0	41.0
49	33.357750091895234	37.0	33.0	40.0	15.0	41.0
50	33.3038955757334	37.0	33.0	40.0	15.0	41.0
51	32.19069629212517	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	2.0
13	2.0
14	0.0
15	9.0
16	10.0
17	20.0
18	51.0
19	79.0
20	144.0
21	220.0
22	362.0
23	589.0
24	1057.0
25	1923.0
26	2747.0
27	3058.0
28	3149.0
29	2905.0
30	3014.0
31	3408.0
32	4047.0
33	5443.0
34	7979.0
35	10533.0
36	13880.0
37	16992.0
38	26285.0
39	19944.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.72559692796196	5.306460821347849	43.509068299664484	29.458873951025705
2	34.753603466209924	4.910724760094789	42.97177447737031	17.363897296324975
3	17.955155126971835	4.938879894887497	58.109069863838634	18.996895114302028
4	15.035624066383551	5.140658360901903	58.34526016126635	21.47845741144819
5	13.37290693945864	6.183962522387242	58.66044125352917	21.78268928462495
6	17.474953661340653	7.210842855243503	61.755159819494295	13.55904366392154
7	64.68955053455652	2.71775259457388	27.106356021679456	5.486340849190149
8	63.97472294565277	3.030587425603967	26.174108225209796	6.8205814035334695
9	58.11845490876954	5.601307649593705	29.174194254788326	7.1060431868484235
10	28.623604952175373	21.38538904921674	35.074259168015764	14.916746830592118
11	18.8936596200621	18.06777566614267	41.298890218436924	21.73967449535831
12	17.136309956750583	16.17434285133307	46.9166216966597	19.77272549525664
13	20.406998115170143	16.7382276342648	47.90361558855963	14.95115866200543
14	16.62326083386124	19.09074556361105	47.241187833853424	17.044805768674284
15	12.81840720145781	18.467422162783603	49.037641851043695	19.67652878471489
16	13.814004051211063	18.915558058234204	45.10061550253005	22.16982238802468
17	13.917239545450991	19.433299703588997	42.66050382049537	23.98895693046464
18	14.883117086256384	18.153023157598366	46.344133955874646	20.6197258002706
19	15.226453313311906	20.647098847985735	45.73175977413325	18.39468806456911
20	14.577321038924474	24.61540867960239	42.95691482289638	17.850355458576757
21	14.102594182836317	24.312740980580777	44.34902982098027	17.23563501560264
22	12.207597193871566	22.746220564197618	42.38129873380102	22.664883508129797
23	12.148940663053425	23.36250518132689	40.79444405340091	23.694110102218783
24	14.00405121106184	23.00039886440956	40.29390832375277	22.70164160077583
25	13.474578259543419	24.740542612014423	37.71927766437515	24.06560146406701
26	12.622885432064008	25.786193034732484	39.66980283584775	21.921118697355762
27	14.243369856799855	24.128168430273025	42.49079092466155	19.137670788265567
28	11.662482500801639	23.56350156026372	40.62394907048951	24.150066868445133
29	13.919585806683715	22.96676912007383	39.339762089111005	23.773882984131454
30	19.646809475767032	23.429764669998356	38.00317527353496	18.920250580699655
31	16.751523114583577	29.110845201504738	33.11434895161227	21.023282732299414
32	15.80128731532969	28.832422201887958	31.473530262859466	23.892760219922888
33	18.842041872942133	23.465740675566817	32.19696080961654	25.49525664187451
34	16.63499214002487	24.817187145616792	31.562688189703042	26.9851325246553
35	15.361754377732417	28.370208739041004	30.414584359822623	25.85345252340396
36	17.865997200128263	28.268537418956225	32.87581239295183	20.98965298796368
37	15.195951917286472	29.96175594190657	32.2172950736335	22.624997067173457
38	17.23719918975779	28.01279494458913	28.579808075831163	26.17019778982192
39	22.21127300313617	21.442481405879732	27.944753368840086	28.401492222144014
40	21.471418627750012	22.660190985664343	29.691935900143125	26.17645448644252
41	16.25098738493544	28.223958455534436	28.3576953457998	27.16735881373032
42	16.42539280323471	27.322212055090212	31.500121223497025	24.75227391817805
43	16.715547109015116	23.117711926045846	35.14230074376481	25.024440221174228
44	18.637917145695003	22.10178081227564	32.290811258925565	26.96949078310379
45	22.018879582052666	19.639770692068854	30.770433980119343	27.570915745759134
46	21.646606133126863	21.846038337908542	33.714209740112466	22.793145788852133
47	15.416500473162682	25.859709220024556	35.9220415601073	22.801748746705456
48	15.867764716923583	24.20246670264267	35.97131304599454	23.958455534439203
49	20.373368370834406	19.54748441691498	36.81674917685335	23.26239803539726
50	18.931981886863284	17.978617739299093	34.61048153101366	28.47891884282396
51	17.249712582998992	18.300055528182508	31.98501521159366	32.46521667722484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	379.0
1	587.5
2	796.0
3	4908.0
4	9020.0
5	5428.0
6	1836.0
7	1754.0
8	1672.0
9	1710.5
10	1749.0
11	1709.0
12	1669.0
13	1590.0
14	1511.0
15	1383.0
16	1255.0
17	1193.5
18	1132.0
19	1011.0
20	890.0
21	859.0
22	828.0
23	832.5
24	837.0
25	836.5
26	858.5
27	881.0
28	928.0
29	975.0
30	1092.5
31	1210.0
32	1351.5
33	1493.0
34	1624.0
35	1755.0
36	1879.0
37	2003.0
38	2287.5
39	2572.0
40	3307.5
41	4043.0
42	5051.5
43	6060.0
44	7062.5
45	8065.0
46	10379.5
47	12694.0
48	14930.5
49	17167.0
50	16431.5
51	15696.0
52	12617.5
53	9539.0
54	7536.0
55	5533.0
56	4625.5
57	3718.0
58	3209.0
59	2700.0
60	2418.0
61	2136.0
62	1872.5
63	1609.0
64	1410.5
65	1212.0
66	1025.5
67	839.0
68	713.0
69	587.0
70	478.0
71	369.0
72	313.5
73	258.0
74	201.0
75	128.0
76	112.0
77	78.0
78	44.0
79	31.0
80	18.0
81	17.0
82	16.0
83	9.5
84	3.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127863.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.31710502647365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2890325349355	45.65589732760846
2	11.815036343641875	13.780374306875329
3	4.526191561837835	7.9186316604490745
4	2.0465091328487515	4.773859521519126
5	1.0567819113268782	3.081423085646356
6	0.5632593943620418	1.9708594354895475
7	0.36477751253922697	1.4890937957032135
8	0.26017219644341927	1.213799144396737
9	0.1810476624735134	0.9502357992538889
>10	0.823431590805461	8.431680783338418
>50	0.05230265804790388	2.135097721780343
>100	0.01609312555320119	2.14370067963367
>500	0.004023281388300297	1.4679774446086826
>1k	0.0	0.0
>5k	0.0013410937961000993	4.98736929369716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6377	4.98736929369716	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	869	0.6796336704128637	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	505	0.3949539741754847	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	503	0.39338980002033425	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	463	0.36210631691732553	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	428	0.33473326920219293	No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	395	0.3089243956422108	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	269	0.21038142386773342	No Hit
TCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	209	0.16345619921322038	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	207	0.16189202505806996	No Hit
TTCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	174	0.13608315149808778	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	150	0.11731306163628259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45126424376090035	0.0
2	0.0	0.0	0.0	1.7229378318982036	0.0
3	0.0	0.0	0.0	2.491729429154642	0.0
4	0.0	0.0	0.0	3.603857253466601	0.0
5	0.0	0.0	0.0	5.9501184861922525	0.0
6	0.0	0.0	0.0	7.815396166209146	0.0
7	0.0	0.0	0.0	9.679109672070888	0.0
8	0.0	0.0	0.0	12.460993407005937	0.0
9	0.0	0.0	0.0	13.799144396737132	0.0
10	0.0	0.0	0.0	16.403494365062606	0.0
11	0.0	0.0	0.0	22.249595269937355	0.0
12	0.0	0.0	0.0	27.022672704378905	0.0
13	0.0	0.0	0.0	28.610309471856596	0.0
14	0.0	0.0	0.0	29.208606086201637	0.0
15	0.0	0.0	0.0	30.003206557018057	0.0
16	0.0	0.0	0.0	31.946692944792474	0.0
17	0.0	0.0	0.0	34.58154430914338	0.0
18	0.0	0.0	0.0	36.865238575663014	0.0
19	0.0	0.0	0.0	38.25266105128145	0.0
20	0.0	0.0	0.0	39.53059133603936	0.0
21	0.0	0.0	0.0	40.90471833133901	0.0
22	0.0	0.0	0.0	42.120863736968474	0.0
23	0.0	0.0	0.0	42.98428787061152	0.0
24	0.0	0.0	0.0	43.4863877744148	0.0
25	0.0	0.0	0.0	43.85084035256485	0.0
26	0.0	0.0	0.0	44.113621610630126	0.0
27	0.0	0.0	0.0	44.375620781617826	0.0
28	0.0	0.0	0.0	44.60007977288191	0.0
29	0.0	0.0	0.0	44.84721928939568	0.0
30	0.0	0.0	0.0	45.0834095868234	0.0
31	0.0	0.0	0.0	45.272674659596596	0.0
32	0.0	0.0	0.0	45.465068080680105	0.0
33	0.0	0.0	0.0	45.6418197602121	0.0
34	0.0	0.0	0.0	45.82248187513198	0.0
35	0.0	0.0	0.0	46.00861859959488	0.0
36	0.0	0.0	0.0	46.17442106004083	0.0
37	0.0	0.0	0.0	46.36290404573645	0.0
38	0.0	0.0	0.0	46.55607955389753	0.0
39	0.0	0.0	0.0	46.93617387359909	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTG	25	3.871318E-5	45.0	42
TCTCTCG	20	7.009762E-4	45.0	1
CCCTACA	20	7.009762E-4	45.0	27
TCTTCCG	20	7.009762E-4	45.0	1
TTGCTCG	25	3.871318E-5	45.0	1
CGTGAGG	25	3.871318E-5	45.0	45
GGCCGAT	35	1.2006421E-7	45.0	8
TCGGGAC	35	1.2006421E-7	45.0	5
CACGACC	25	3.871318E-5	45.0	27
GCTTGCG	20	7.009762E-4	45.0	1
GGAAGAT	20	7.009762E-4	45.0	8
GCCCTAC	20	7.009762E-4	45.0	26
GTCAAGC	25	3.871318E-5	45.0	16
CGGGACT	20	7.009762E-4	45.0	6
AAACACG	25	3.871318E-5	45.0	40
CGGGACC	35	1.2006421E-7	45.0	6
CTATTGG	25	3.871318E-5	45.0	2
CGAGACA	25	3.871318E-5	45.0	22
TTGCAGG	20	7.009762E-4	45.0	2
GGCTGCG	20	7.009762E-4	45.0	1
>>END_MODULE