##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127102
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947742757785086	33.0	31.0	34.0	30.0	34.0
2	32.178486569841546	34.0	31.0	34.0	30.0	34.0
3	32.3008921968183	34.0	31.0	34.0	30.0	34.0
4	35.8489874274205	37.0	35.0	37.0	35.0	37.0
5	35.93236927821749	37.0	35.0	37.0	35.0	37.0
6	35.9284511652059	37.0	35.0	37.0	35.0	37.0
7	35.899167597677454	37.0	35.0	37.0	35.0	37.0
8	35.78413400261208	37.0	35.0	37.0	35.0	37.0
9	37.58391685418011	39.0	37.0	39.0	35.0	39.0
10	37.1223584207959	39.0	37.0	39.0	33.0	39.0
11	37.10852700980315	39.0	37.0	39.0	34.0	39.0
12	37.13481298484682	39.0	37.0	39.0	34.0	39.0
13	37.08522289185064	39.0	37.0	39.0	33.0	39.0
14	38.063791285739015	40.0	37.0	41.0	33.0	41.0
15	38.11646551588488	40.0	37.0	41.0	33.0	41.0
16	38.29668297902472	40.0	38.0	41.0	33.0	41.0
17	38.35783071863543	40.0	38.0	41.0	34.0	41.0
18	38.16249940992274	39.0	38.0	41.0	34.0	41.0
19	37.926287548583026	39.0	37.0	41.0	34.0	41.0
20	37.44387185095435	39.0	35.0	40.0	34.0	41.0
21	37.358389325108966	39.0	35.0	40.0	33.0	41.0
22	37.36782269358468	39.0	35.0	40.0	33.0	41.0
23	37.27067237336942	39.0	35.0	40.0	33.0	41.0
24	37.145615332567544	39.0	35.0	40.0	33.0	41.0
25	37.062729146669604	38.0	35.0	40.0	33.0	41.0
26	37.05402747399726	38.0	35.0	40.0	33.0	41.0
27	37.00762379820931	38.0	35.0	40.0	33.0	41.0
28	36.95015027300908	38.0	35.0	40.0	33.0	41.0
29	36.79585687085962	38.0	35.0	40.0	33.0	41.0
30	36.56040030841371	38.0	35.0	40.0	32.0	41.0
31	36.26886280310302	38.0	35.0	40.0	31.0	41.0
32	36.0116127204922	38.0	35.0	40.0	30.0	41.0
33	35.58756746550015	38.0	35.0	40.0	27.0	41.0
34	35.25368601595569	38.0	35.0	40.0	25.0	41.0
35	34.99697880442479	38.0	35.0	40.0	23.0	41.0
36	34.825510220138156	38.0	35.0	40.0	22.0	41.0
37	34.73375713993485	38.0	35.0	40.0	22.0	41.0
38	34.62999795439883	38.0	34.0	40.0	21.0	41.0
39	34.631579361457725	38.0	35.0	40.0	21.0	41.0
40	34.42813645733348	38.0	34.0	40.0	20.0	41.0
41	34.29297729382701	38.0	34.0	40.0	18.0	41.0
42	34.14608739437617	38.0	34.0	40.0	18.0	41.0
43	34.11280703686803	38.0	34.0	40.0	18.0	41.0
44	33.986884549417006	38.0	33.0	40.0	17.0	41.0
45	33.971345848216394	38.0	33.0	40.0	18.0	41.0
46	33.680665921858036	37.0	33.0	40.0	17.0	41.0
47	33.607032147409164	37.0	33.0	40.0	17.0	41.0
48	33.57114758225677	37.0	33.0	40.0	17.0	41.0
49	33.61282277226165	37.0	33.0	40.0	17.0	41.0
50	33.44498906390143	37.0	33.0	40.0	15.0	41.0
51	32.499071611776365	36.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	5.0
12	5.0
13	0.0
14	5.0
15	9.0
16	8.0
17	15.0
18	35.0
19	75.0
20	138.0
21	248.0
22	318.0
23	561.0
24	935.0
25	1805.0
26	2729.0
27	3030.0
28	3032.0
29	2755.0
30	2882.0
31	3119.0
32	3876.0
33	5085.0
34	7582.0
35	9885.0
36	13175.0
37	15615.0
38	26514.0
39	23654.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.907601768658242	5.393306163553682	43.248729366965115	29.450362700822964
2	35.01125080643892	4.959796069298673	42.94975688816856	17.079196236093846
3	18.41041053641957	5.0298185709115515	57.84330695032336	18.71646394234552
4	15.373479567591383	5.08253213954147	58.01875658919607	21.525231703671068
5	13.33023870592123	6.0305896051989745	58.91803433462888	21.721137354250917
6	17.55991251121147	7.221758902298942	61.74647133798052	13.471857248509073
7	64.66617362433321	2.7615615804629354	27.241900206133657	5.330364589070196
8	64.19883243379333	3.0274897326556625	26.13019464681909	6.643483186731916
9	58.26580226904377	5.622256140737361	29.08844864754292	7.023492942675961
10	28.743056757564787	21.42452518449749	35.045081902723794	14.787336155213923
11	18.9941936397539	18.206637189029284	41.25190791647653	21.547261254740288
12	17.22789570581108	15.89510786612327	46.60430205661595	20.272694371449703
13	20.471747100753728	16.832937325927208	47.84425107394061	14.851064499378452
14	17.02569589778288	19.400953564853424	47.23922518921811	16.334125348145584
15	13.05722962659911	18.28216707840947	49.05902346147189	19.601579833519537
16	13.890418718824252	18.93675945303772	45.10078519614168	22.07203663199635
17	14.230303221035074	19.45052005475917	42.66809334235496	23.651083381850796
18	15.139808972321442	18.103570360812576	46.05120297084231	20.705417696023666
19	15.268052430331544	20.493776651822945	45.97803339050526	18.260137527340248
20	14.815659863731492	24.50000786769681	42.937955342952904	17.746376925618794
21	14.346745133829522	24.452014917153154	44.23455177731271	16.966688171704618
22	12.221680225330836	22.817894289625652	42.54299696306903	22.41742852197448
23	12.504130540825479	23.337162279114413	40.75939009614326	23.399317083916856
24	14.115434847602712	23.217573287595787	40.21337193749901	22.453619927302483
25	13.610328712372741	24.95554751302104	37.532060864502526	23.902062910103698
26	12.511211467954872	25.757265818004438	39.803464933675315	21.928057780365375
27	14.331796509889694	24.226212018693648	42.35889285770483	19.083098613711822
28	11.820427687998615	23.4669792764866	40.75545624773804	23.957136787776744
29	13.814888829444069	23.209705590785354	39.4564995043351	23.518906075435478
30	19.740051297383204	23.36312567858885	38.036380230051456	18.860442793976492
31	17.07604915736967	29.05776462998222	33.23079101823732	20.635395194410787
32	15.79597488631178	28.84455004641941	31.758744945004803	23.600730122264007
33	18.955641925382764	23.41583924721877	32.02624663655961	25.602272190838853
34	17.108306714292457	24.57711129643908	31.933407814196475	26.38117417507199
35	15.619738477758021	28.3260688266117	30.272537017513496	25.78165567811679
36	17.810892039464367	28.55108495539016	32.821670784094664	20.81635222105081
37	15.460811002187219	29.559723686488017	32.52033799625498	22.459127315069786
38	17.413573350537366	28.065648062186277	28.580195433588774	25.94058315368759
39	22.03269814794417	21.27425217541817	28.164781041997767	28.528268634639897
40	21.843086654812673	22.60310616670076	29.853975547198313	25.699831631288255
41	16.246793913549748	28.099479158471148	28.573901276140422	27.07982565183868
42	16.166543406083303	27.890985192994606	31.31579361457727	24.626677786344825
43	16.761341284952245	23.13181539236204	35.15995027615616	24.94689304652956
44	18.651948828499947	22.094852952746614	32.49830844518576	26.754889773567687
45	21.815549715976147	19.740051297383204	30.450346965429343	27.994052021211314
46	21.877704520778586	21.786439237777532	33.76815471039008	22.5677015310538
47	15.530833503800098	25.675441771175905	36.258280750892986	22.535443974131013
48	15.827445673553525	24.56609652090447	36.04663970669226	23.559818098849743
49	20.41982030180485	19.47963053295778	36.82632846060645	23.274220704630928
50	19.310475051533412	17.785715409670974	34.62179981432236	28.282009724473255
51	16.913187833393653	18.577205708800808	32.158423942974935	32.351182514830604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	326.0
1	582.0
2	838.0
3	4829.0
4	8820.0
5	5391.5
6	1963.0
7	1840.0
8	1717.0
9	1745.5
10	1774.0
11	1743.5
12	1713.0
13	1622.0
14	1531.0
15	1435.5
16	1340.0
17	1199.5
18	1059.0
19	990.5
20	922.0
21	841.5
22	761.0
23	766.0
24	771.0
25	801.5
26	855.0
27	878.0
28	902.5
29	927.0
30	1092.0
31	1257.0
32	1360.0
33	1463.0
34	1600.5
35	1738.0
36	1909.0
37	2080.0
38	2377.5
39	2675.0
40	3412.0
41	4149.0
42	5105.0
43	6061.0
44	7087.5
45	8114.0
46	10213.0
47	12312.0
48	14526.5
49	16741.0
50	16062.5
51	15384.0
52	12450.5
53	9517.0
54	7491.5
55	5466.0
56	4573.5
57	3681.0
58	3143.0
59	2605.0
60	2433.0
61	2261.0
62	1955.0
63	1649.0
64	1469.0
65	1289.0
66	1126.0
67	963.0
68	759.5
69	556.0
70	466.0
71	376.0
72	307.0
73	238.0
74	195.5
75	127.0
76	101.0
77	74.0
78	47.0
79	37.5
80	28.0
81	24.0
82	20.0
83	12.0
84	4.0
85	2.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.66390772765181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50944838592868	46.05671035860962
2	11.46144870780414	13.447467388396719
3	4.629642047664391	8.147786816887225
4	2.0492737684910747	4.808736290538308
5	1.09974115848343	3.2257556922786423
6	0.627657148988104	2.209249264370348
7	0.3714979279267197	1.5255464115434847
8	0.22799511822217453	1.070006766219257
9	0.14618510521304132	0.7718210571037435
>10	0.8020063570403552	8.181617913172099
>50	0.05230476241567533	2.0149171531525862
>100	0.020117216313721285	2.9771364730688736
>500	0.0013411477542480856	0.6726880772922534
>1k	0.0	0.0
>5k	0.0013411477542480856	4.890560337366839
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6216	4.890560337366839	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	855	0.6726880772922534	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	484	0.3807965256250885	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	481	0.3784362165819578	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	448	0.35247281710751993	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	441	0.34696542934021496	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	412	0.3241491085899514	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.17780994791584712	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218	0.17151579046749854	No Hit
TCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	195	0.15342008780349642	No Hit
TTCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	175	0.13768469418262497	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.867696810435714E-4	0.0	0.0	0.4335100942550078	0.0
2	7.867696810435714E-4	0.0	0.0	1.649856021148369	0.0
3	7.867696810435714E-4	0.0	0.0	2.3980739878208053	0.0
4	7.867696810435714E-4	0.0	0.0	3.4625733662727574	0.0
5	7.867696810435714E-4	0.0	0.0	5.773315919497726	0.0
6	7.867696810435714E-4	0.0	0.0	7.622224669950119	0.0
7	7.867696810435714E-4	0.0	0.0	9.37357397995311	0.0
8	7.867696810435714E-4	0.0	0.0	12.03521581092351	0.0
9	7.867696810435714E-4	0.0	0.0	13.308209154852008	0.0
10	7.867696810435714E-4	0.0	0.0	15.77394533524256	0.0
11	7.867696810435714E-4	0.0	0.0	21.45127535365297	0.0
12	7.867696810435714E-4	0.0	0.0	26.294629510157197	0.0
13	7.867696810435714E-4	0.0	0.0	27.84613932117512	0.0
14	7.867696810435714E-4	0.0	0.0	28.394517788862487	0.0
15	7.867696810435714E-4	0.0	0.0	29.18128746990606	0.0
16	7.867696810435714E-4	0.0	0.0	31.171814762946294	0.0
17	7.867696810435714E-4	0.0	0.0	33.65171279759563	0.0
18	7.867696810435714E-4	0.0	0.0	35.9317713332599	0.0
19	7.867696810435714E-4	0.0	0.0	37.285015184654846	0.0
20	7.867696810435714E-4	0.0	0.0	38.45022108228037	0.0
21	7.867696810435714E-4	0.0	0.0	39.85539173262419	0.0
22	7.867696810435714E-4	0.0	0.0	41.07567150792277	0.0
23	7.867696810435714E-4	0.0	0.0	41.915941527277305	0.0
24	7.867696810435714E-4	0.0	0.0	42.424981510912495	0.0
25	7.867696810435714E-4	0.0	0.0	42.75070415886454	0.0
26	7.867696810435714E-4	0.0	0.0	43.00797784456578	0.0
27	7.867696810435714E-4	0.0	0.0	43.28413400261208	0.0
28	7.867696810435714E-4	0.0	0.0	43.50285597394219	0.0
29	7.867696810435714E-4	0.0	0.0	43.734166260169	0.0
30	7.867696810435714E-4	0.0	0.0	43.97885163097355	0.0
31	7.867696810435714E-4	0.0	0.0	44.16138219697566	0.0
32	7.867696810435714E-4	0.0	0.0	44.35020692042612	0.0
33	7.867696810435714E-4	0.0	0.0	44.56735535239414	0.0
34	7.867696810435714E-4	0.0	0.0	44.745952069991034	0.0
35	7.867696810435714E-4	0.0	0.0	44.91196047269123	0.0
36	7.867696810435714E-4	0.0	0.0	45.09370426901229	0.0
37	7.867696810435714E-4	0.0	0.0	45.262859750436654	0.0
38	7.867696810435714E-4	0.0	0.0	45.453258013249204	0.0
39	7.867696810435714E-4	0.0	0.0	45.83484130855533	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	35	1.2005512E-7	45.000004	8
GGATGTA	20	7.009605E-4	45.0	8
GGATAAA	20	7.009605E-4	45.0	8
GCGTTGG	25	3.8711893E-5	45.0	2
ATACTAT	20	7.009605E-4	45.0	45
GCCGATT	20	7.009605E-4	45.0	9
GTTCGTG	20	7.009605E-4	45.0	1
GTCGTTG	20	7.009605E-4	45.0	1
GCGATGT	20	7.009605E-4	45.0	9
CACAAAT	25	3.8711893E-5	45.0	32
TACAGGG	20	7.009605E-4	45.0	3
GGACAGT	25	3.8711893E-5	45.0	8
TGCGTTG	20	7.009605E-4	45.0	1
CGTTTTT	2015	0.0	44.106697	1
TGGGTCT	70	0.0	41.785717	6
GTCTTTG	65	0.0	41.538464	1
GGCGATT	55	6.002665E-11	40.909092	8
CCGGGAC	50	1.0659278E-9	40.5	5
GCACCGA	45	1.9052095E-8	40.000004	9
GGGCCGA	45	1.9052095E-8	40.000004	7
>>END_MODULE