FastQCFastQC Report
Sat 14 Jan 2017
SRR2933716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420528
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104412.48283110756002No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC25910.6161301982269908No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC19020.4522885515352129TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT17860.42470418141003685TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC12530.297958756610737TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC12320.2929650344329034No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC9330.2218639424723205TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG6160.1464825172164517No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT5170.1229406840923791No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG5140.12222729520983144No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG4440.10558155461705283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGG650.045.0000042
CGGGTCC453.8380676E-1045.06
GTTCTCG351.2094642E-745.01
CGTATGG351.2094642E-745.02
TGCCTCG207.0281787E-445.01
GCGATGC406.7975634E-945.09
CGATGCG207.0281787E-445.010
TGGGCGC207.0281787E-445.06
GTCTTCG253.886572E-545.01
TACGGGA302.1622072E-644.9999964
TCGAGGG302.1622072E-644.9999963
GTGAAGT302.1622072E-644.99999615
CGTTTTT54900.044.3852461
GGACCGA750.042.0000048
GACCGAT700.041.7857139
TGGGTCC1750.041.1428576
TCTTGCG556.002665E-1140.9090921
GGATCTA556.002665E-1140.9090928
TGTTTCG501.0786607E-940.51
GCTTGCG501.0786607E-940.51
Produced by FastQC (version 0.11.5)