##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420528
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03195268804931	33.0	31.0	34.0	30.0	34.0
2	32.2283438914888	34.0	31.0	34.0	30.0	34.0
3	32.35888454514325	34.0	31.0	34.0	30.0	34.0
4	35.93212342578853	37.0	35.0	37.0	35.0	37.0
5	35.95125413765552	37.0	35.0	37.0	35.0	37.0
6	35.91039360042613	37.0	35.0	37.0	35.0	37.0
7	35.99267587413918	37.0	35.0	37.0	35.0	37.0
8	35.83435823536126	37.0	35.0	37.0	35.0	37.0
9	37.6575947380436	39.0	37.0	39.0	35.0	39.0
10	36.86542394323327	39.0	37.0	39.0	32.0	39.0
11	36.88800983525473	39.0	37.0	39.0	33.0	39.0
12	37.091266217707265	39.0	37.0	39.0	34.0	39.0
13	37.11350492713922	39.0	37.0	39.0	33.0	39.0
14	38.16016531598372	40.0	37.0	41.0	33.0	41.0
15	38.19874063082601	40.0	37.0	41.0	33.0	41.0
16	38.29147167370544	40.0	38.0	41.0	34.0	41.0
17	38.304521934330175	40.0	38.0	41.0	34.0	41.0
18	38.123390119088384	39.0	38.0	41.0	34.0	41.0
19	37.89219505003234	39.0	37.0	41.0	34.0	41.0
20	37.48935148194651	39.0	35.0	40.0	33.0	41.0
21	37.374983354259406	39.0	35.0	40.0	33.0	41.0
22	37.41547055130693	39.0	35.0	40.0	33.0	41.0
23	37.3495177491154	39.0	35.0	40.0	33.0	41.0
24	37.25792099455922	39.0	35.0	40.0	33.0	41.0
25	37.18051592283986	39.0	35.0	40.0	33.0	41.0
26	37.13484001065327	39.0	35.0	40.0	33.0	41.0
27	37.09167284937032	39.0	35.0	40.0	33.0	41.0
28	37.00846079214701	39.0	35.0	40.0	33.0	41.0
29	36.92028117033824	39.0	35.0	40.0	32.0	41.0
30	36.792993569988205	39.0	35.0	40.0	32.0	41.0
31	36.460937202754636	38.0	35.0	40.0	31.0	41.0
32	36.017722957805425	38.0	35.0	40.0	30.0	41.0
33	35.664604972796106	38.0	35.0	40.0	27.0	41.0
34	35.35863248107142	39.0	35.0	40.0	24.0	41.0
35	34.987772514553136	39.0	35.0	40.0	22.0	41.0
36	34.845256439523645	38.0	35.0	40.0	21.0	41.0
37	34.64803057109158	38.0	35.0	40.0	19.0	41.0
38	34.32857027356086	38.0	34.0	40.0	18.0	41.0
39	34.27080241981509	38.0	34.0	40.0	18.0	41.0
40	34.23413423125214	38.0	33.0	40.0	18.0	41.0
41	33.9284090476734	38.0	33.0	40.0	16.0	41.0
42	33.761219229159536	38.0	33.0	40.0	15.0	41.0
43	33.81254042537001	38.0	33.0	40.0	15.0	41.0
44	33.84992200281551	38.0	33.0	40.0	15.0	41.0
45	33.91182037819122	38.0	33.0	40.0	15.0	41.0
46	33.61927862116197	38.0	33.0	40.0	15.0	41.0
47	33.301366377506376	37.0	32.0	40.0	14.0	41.0
48	33.20217203135106	37.0	32.0	40.0	13.0	41.0
49	33.31841399383632	38.0	33.0	40.0	13.0	41.0
50	33.27850939770955	38.0	32.0	40.0	12.0	41.0
51	32.00667494197771	36.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	4.0
13	5.0
14	3.0
15	9.0
16	20.0
17	60.0
18	87.0
19	241.0
20	429.0
21	680.0
22	1239.0
23	2148.0
24	3973.0
25	7220.0
26	10478.0
27	10516.0
28	9031.0
29	8359.0
30	8812.0
31	9992.0
32	12156.0
33	15665.0
34	22777.0
35	30550.0
36	42025.0
37	61136.0
38	95790.0
39	67104.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.399288513487804	4.7846992352471185	47.15976106228361	26.65625118898147
2	32.97901685500133	4.551183274359852	45.12588935814024	17.343910512498574
3	17.608577787923753	4.83059392002435	58.47934025796142	19.081488034090476
4	14.31462352090705	4.535013126355439	59.81171289426626	21.338650458471257
5	13.364627325647756	5.91542061408515	59.6652303770498	21.054721683217288
6	17.248078605943004	6.8977571053532705	62.8638283300993	12.99033595860442
7	69.87073393448236	1.9575390937107635	23.496414031883727	4.675312939923145
8	70.09973176578016	2.127087851462923	21.882966175855113	5.8902142069018
9	64.119154967089	4.910493474869688	24.80548263135867	6.164868926682646
10	28.254717878476583	21.773104287942775	33.86290568047787	16.109272153102765
11	18.046360765513832	20.575562150439445	38.31421451128106	23.063862572765668
12	15.484343491991021	16.722548795799565	45.71847772324316	22.07462998896625
13	20.385800707681774	18.1666856903702	47.08176387779173	14.365749724156299
14	18.736445611231595	19.899031693490087	44.56897043716471	16.79555225811361
15	13.807166229121485	20.121609024844958	46.00954038732261	20.061684358710956
16	12.735893923829092	19.36422782787353	44.08695734885668	23.812920899440705
17	12.94182551459118	19.612962751588476	42.19172088422174	25.2534908495986
18	13.771021192405739	17.81427158239166	46.37503329148119	22.039673933721417
19	14.753357683673856	20.90586120305901	46.45992656850436	17.880854544762776
20	14.468477723243161	23.87902826922345	44.04201384925617	17.610480158277213
21	13.505878324392192	24.983829851995587	43.83964920290682	17.670642620705397
22	12.147348095727276	23.219619145455237	41.47381387208462	23.15921888673287
23	11.437763953886543	23.992457101548528	39.00429935699882	25.56547958756611
24	12.631739146977134	23.1451889053761	39.78522238709432	24.43784956055245
25	11.574496823041509	25.37857170033862	37.84504242285888	25.201889053760983
26	11.109367271620439	26.431295894684776	40.74829737853366	21.711039455161128
27	13.216955826960392	24.897985389795686	41.62410113000799	20.26095765323593
28	11.908362820073812	21.585720808126926	42.18078225468934	24.32513411710992
29	13.800745729178557	19.51689304873873	41.656679222311	25.025681999771717
30	20.349893467260205	22.07177643343606	36.665572803713424	20.912757295590307
31	19.08743294144504	26.832696039264924	32.60448769166381	21.475383327626222
32	16.455027964844195	26.244388007457292	33.640803941711376	23.65978008598714
33	20.896824943880073	21.510577179165242	31.221940037286462	26.370657839668226
34	21.60189095613134	18.837271239965	33.52523494273865	26.035602861165014
35	17.8982136742381	18.6736673895674	37.666695202221966	25.761423733972528
36	21.2589886999201	21.026423924209563	34.237197047521214	23.477390328349124
37	24.444032264201194	21.709850473690217	32.21664193585207	21.629475326256514
38	24.64663470684473	21.044972035155805	34.765104820606474	19.54328843739299
39	28.33271506296846	19.509045771030703	33.252482593311264	18.905756572689572
40	30.49428337708785	17.783120267853747	33.043935243313165	18.678661111745235
41	23.4735855876422	20.29044439371457	34.94440322642012	21.29156679222311
42	20.527527299014572	23.880217250694365	35.768605182056845	19.823650268234218
43	20.671156260700833	22.150487006810486	36.836072746642316	20.342283985846365
44	22.68433968725031	18.484143743103907	33.96040216109272	24.87111440855306
45	27.74012669786554	15.42180306662101	32.94429859605068	23.89377163946277
46	28.370286877449303	17.22881710611422	35.63020773884259	18.770688277593884
47	21.18622303390024	22.563063577217214	38.294715215158085	17.95599817372446
48	19.089573108092683	22.638920595061446	39.48678803789522	18.784718258950655
49	24.668749762203706	17.868013544876916	38.084978883689075	19.378257809230302
50	23.475963550584027	15.465081992162233	34.40389224974318	26.65506220751056
51	20.696362667884184	15.172592550317695	31.90132404976601	32.22972073203211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	881.5
2	1257.0
3	8942.0
4	16627.0
5	10812.5
6	4998.0
7	4912.0
8	4826.0
9	4903.0
10	4980.0
11	4961.0
12	4942.0
13	4777.0
14	4612.0
15	4462.5
16	4313.0
17	4053.5
18	3794.0
19	3528.5
20	3263.0
21	3115.0
22	2967.0
23	2887.5
24	2808.0
25	2799.0
26	2889.5
27	2989.0
28	3132.0
29	3275.0
30	3561.5
31	3848.0
32	4540.5
33	5233.0
34	5792.0
35	6351.0
36	7268.0
37	8185.0
38	9184.5
39	10184.0
40	12344.0
41	14504.0
42	18197.0
43	21890.0
44	26547.5
45	31205.0
46	39936.0
47	48667.0
48	56844.5
49	65022.0
50	61851.5
51	58681.0
52	47012.5
53	35344.0
54	26383.0
55	17422.0
56	13322.5
57	9223.0
58	7392.0
59	5561.0
60	4663.0
61	3765.0
62	3197.0
63	2629.0
64	2106.5
65	1584.0
66	1284.5
67	985.0
68	737.5
69	490.0
70	409.5
71	329.0
72	278.0
73	227.0
74	164.0
75	84.5
76	68.0
77	53.0
78	38.0
79	30.0
80	22.0
81	20.0
82	18.0
83	11.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14495934814108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90012808096267	35.57918662280367
2	11.59340532169214	11.163280558387811
3	5.297596606681751	7.651577198146291
4	2.89167894454563	5.5687906093209945
5	1.7884449271239804	4.3052304156386585
6	1.1159745585013514	3.223712985156435
7	0.766398217181371	2.582874770747943
8	0.5578412984494138	2.148579730924901
9	0.3712602718105457	1.6086879624508679
>10	1.550405843995132	12.81826281994179
>50	0.10663621501808818	3.4994364166017333
>100	0.055292852231562405	4.67241194576646
>500	0.0019747447225558	0.6132273283181131
>1k	0.00246843090319475	2.0830714361937765
>5k	0.0	0.0
>10k+	4.9368618063895E-4	2.4816691996005504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10441	2.48283110756002	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	2591	0.6161301982269908	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1902	0.4522885515352129	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1786	0.42470418141003685	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1253	0.297958756610737	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC	1232	0.2929650344329034	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	933	0.2218639424723205	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	616	0.1464825172164517	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	517	0.1229406840923791	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG	514	0.12222729520983144	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	444	0.10558155461705283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3779629418255147E-4	0.0	0.0	0.4584712551839592	0.0
2	2.3779629418255147E-4	0.0	0.0	1.5452003195982194	0.0
3	2.3779629418255147E-4	0.0	0.0	2.250979720732032	0.0
4	2.3779629418255147E-4	0.0	0.0	3.332001674085911	0.0
5	2.3779629418255147E-4	0.0	0.0	5.4771620439067075	0.0
6	2.3779629418255147E-4	0.0	0.0	7.263012213217669	0.0
7	2.3779629418255147E-4	0.0	0.0	8.87265532853936	0.0
8	2.3779629418255147E-4	0.0	0.0	11.529553323441007	0.0
9	2.3779629418255147E-4	0.0	0.0	12.807946200966404	0.0
10	2.3779629418255147E-4	0.0	0.0	15.833428451851006	0.0
11	2.3779629418255147E-4	0.0	0.0	22.972786592093748	0.0
12	2.3779629418255147E-4	0.0	0.0	29.068694593463455	0.0
13	2.3779629418255147E-4	0.0	0.0	31.057622798006317	0.0
14	2.3779629418255147E-4	0.0	0.0	31.722739032834912	0.0
15	2.3779629418255147E-4	0.0	0.0	32.70031959821938	0.0
16	2.3779629418255147E-4	0.0	0.0	35.20265000190237	0.0
17	2.3779629418255147E-4	0.0	0.0	38.70681619297645	0.0
18	2.3779629418255147E-4	0.0	0.0	41.95083323821481	0.0
19	2.3779629418255147E-4	0.0	0.0	43.97138834988395	0.0
20	2.3779629418255147E-4	0.0	0.0	45.543697447018985	0.0
21	2.3779629418255147E-4	0.0	0.0	47.493389263021726	0.0
22	2.3779629418255147E-4	0.0	0.0	49.12490963740821	0.0
23	2.3779629418255147E-4	0.0	0.0	50.39236388540121	0.0
24	2.3779629418255147E-4	0.0	0.0	51.09196058288627	0.0
25	4.7559258836510294E-4	0.0	0.0	51.561846060190994	0.0
26	4.7559258836510294E-4	0.0	0.0	51.88168207586653	0.0
27	4.7559258836510294E-4	0.0	0.0	52.164897462237946	0.0
28	4.7559258836510294E-4	0.0	0.0	52.42837575619222	0.0
29	4.7559258836510294E-4	0.0	0.0	52.698750142677774	0.0
30	4.7559258836510294E-4	0.0	0.0	52.9560457329833	0.0
31	0.0019023703534604118	0.0	0.0	53.18242780504509	0.0
32	0.0021401666476429633	0.0	0.0	53.3826522847468	0.0
33	0.0021401666476429633	0.0	0.0	53.57217593121029	0.0
34	0.0021401666476429633	0.0	0.0	53.758846022143594	0.0
35	0.0021401666476429633	0.0	0.0	53.95645474260929	0.0
36	0.0021401666476429633	0.0	0.0	54.13361298177529	0.0
37	0.0021401666476429633	0.0	0.0	54.323136628238785	0.0
38	0.0021401666476429633	0.0	0.0	54.51313586729064	0.0
39	0.0021401666476429633	0.0	0.0	54.73167066164441	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCGG	65	0.0	45.000004	2
CGGGTCC	45	3.8380676E-10	45.0	6
GTTCTCG	35	1.2094642E-7	45.0	1
CGTATGG	35	1.2094642E-7	45.0	2
TGCCTCG	20	7.0281787E-4	45.0	1
GCGATGC	40	6.7975634E-9	45.0	9
CGATGCG	20	7.0281787E-4	45.0	10
TGGGCGC	20	7.0281787E-4	45.0	6
GTCTTCG	25	3.886572E-5	45.0	1
TACGGGA	30	2.1622072E-6	44.999996	4
TCGAGGG	30	2.1622072E-6	44.999996	3
GTGAAGT	30	2.1622072E-6	44.999996	15
CGTTTTT	5490	0.0	44.385246	1
GGACCGA	75	0.0	42.000004	8
GACCGAT	70	0.0	41.785713	9
TGGGTCC	175	0.0	41.142857	6
TCTTGCG	55	6.002665E-11	40.909092	1
GGATCTA	55	6.002665E-11	40.909092	8
TGTTTCG	50	1.0786607E-9	40.5	1
GCTTGCG	50	1.0786607E-9	40.5	1
>>END_MODULE