FastQCFastQC Report
Sat 14 Jan 2017
SRR2933715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422876
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101462.399284896754604No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC25340.5992300343363066No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC18860.44599362460863234TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT18390.4348792553845572TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC13270.31380357362441946TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC12790.3024527284594065No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC9510.2248886198318183TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG6380.15087165031829664No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT5230.12367691711045317No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG5090.1203662539373244No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG4460.10546826965824496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG207.028224E-445.01
TCGTTAG253.88661E-545.01
TTTACGG351.2095006E-745.02
ACGTTGT207.028224E-445.010
CGTTGTC207.028224E-445.011
TTCCGGA207.028224E-445.03
TACGGGA453.8380676E-1045.04
CGTTAGG502.1827873E-1145.02
TTGCGGA253.88661E-545.03
TGGGACG253.88661E-545.06
CCGTTTG253.88661E-545.01
AGTCGCG207.028224E-445.01
TGGTACG207.028224E-445.06
AGGGTGC302.1622345E-644.9999966
TGTTGCG302.1622345E-644.9999961
CTACGGG302.1622345E-644.9999963
CGTTTTT55850.044.073411
TCGGGAC950.042.631585
TTCGTTG750.042.01
TAGTTGG650.041.538462
Produced by FastQC (version 0.11.5)