##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422876
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964263755805483	33.0	31.0	34.0	30.0	34.0
2	32.167372941476934	34.0	31.0	34.0	30.0	34.0
3	32.31494338765974	34.0	31.0	34.0	30.0	34.0
4	35.85119041988668	37.0	35.0	37.0	35.0	37.0
5	35.93394044589903	37.0	35.0	37.0	35.0	37.0
6	35.91734692912343	37.0	35.0	37.0	35.0	37.0
7	35.92768329250182	37.0	35.0	37.0	35.0	37.0
8	35.80824638901238	37.0	35.0	37.0	35.0	37.0
9	37.629789820183696	39.0	37.0	39.0	35.0	39.0
10	36.911983654782965	39.0	37.0	39.0	32.0	39.0
11	36.92485267548879	39.0	37.0	39.0	33.0	39.0
12	37.085441122220224	39.0	37.0	39.0	33.0	39.0
13	37.12691900226071	39.0	37.0	39.0	33.0	39.0
14	38.20537226042622	40.0	38.0	41.0	34.0	41.0
15	38.219423660836746	40.0	37.0	41.0	33.0	41.0
16	38.37556163035973	40.0	38.0	41.0	34.0	41.0
17	38.43535930154466	40.0	38.0	41.0	34.0	41.0
18	38.30381010036039	39.0	38.0	41.0	34.0	41.0
19	38.112680785856845	39.0	37.0	41.0	34.0	41.0
20	37.67819881005307	39.0	35.0	41.0	34.0	41.0
21	37.60569292180214	39.0	35.0	40.0	34.0	41.0
22	37.61690188140258	39.0	35.0	40.0	34.0	41.0
23	37.51136739848088	39.0	35.0	40.0	33.0	41.0
24	37.4230885649694	39.0	35.0	40.0	33.0	41.0
25	37.35570001608036	39.0	35.0	40.0	33.0	41.0
26	37.35762020072078	39.0	35.0	40.0	33.0	41.0
27	37.312330328512374	39.0	35.0	40.0	33.0	41.0
28	37.29375750811113	39.0	35.0	40.0	33.0	41.0
29	37.12280195612898	39.0	35.0	40.0	33.0	41.0
30	36.92612491605104	39.0	35.0	40.0	32.0	41.0
31	36.60100833341216	39.0	35.0	40.0	31.0	41.0
32	36.24572924450666	39.0	35.0	40.0	30.0	41.0
33	35.790297865095205	39.0	35.0	40.0	27.0	41.0
34	35.47292586952204	39.0	35.0	40.0	24.0	41.0
35	35.16111578807972	39.0	35.0	40.0	23.0	41.0
36	34.97902458403882	39.0	35.0	40.0	21.0	41.0
37	34.84147362347355	39.0	35.0	40.0	20.0	41.0
38	34.50811112477417	38.0	34.0	40.0	18.0	41.0
39	34.49586167103359	38.0	34.0	40.0	18.0	41.0
40	34.44226439902005	38.0	34.0	40.0	18.0	41.0
41	34.1724831865606	38.0	33.0	40.0	17.0	41.0
42	33.951080222098206	38.0	33.0	40.0	15.0	41.0
43	34.0185586318448	38.0	33.0	40.0	15.0	41.0
44	34.06217425439136	38.0	33.0	40.0	15.0	41.0
45	34.07732999744606	38.0	33.0	40.0	15.0	41.0
46	33.75746318069599	38.0	33.0	40.0	15.0	41.0
47	33.59148308251118	38.0	33.0	40.0	15.0	41.0
48	33.44374946792913	38.0	33.0	40.0	14.0	41.0
49	33.54551925387111	38.0	33.0	40.0	13.0	41.0
50	33.42237204286836	38.0	33.0	40.0	13.0	41.0
51	32.29769719728715	36.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	5.0
12	5.0
13	8.0
14	5.0
15	14.0
16	18.0
17	42.0
18	93.0
19	202.0
20	444.0
21	712.0
22	1249.0
23	2123.0
24	3854.0
25	7123.0
26	10552.0
27	10467.0
28	9058.0
29	8138.0
30	8330.0
31	9565.0
32	11598.0
33	15029.0
34	21588.0
35	28933.0
36	39564.0
37	57318.0
38	97758.0
39	79044.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.4618469716891	5.026769076514155	46.89578032330991	26.615603628486838
2	32.87322997758208	4.624287025038073	45.044646657649054	17.457836339730797
3	17.73025662369111	4.869749051731477	58.20807990994996	19.191914414627455
4	14.6253275191782	4.580538975964585	59.43822775470824	21.355905750148978
5	13.405821091762125	5.909770239975785	59.56545181093276	21.118956857329334
6	17.411487055306992	6.972966070431995	62.65784769057596	12.957699183685051
7	69.92026031271578	1.9291707261703195	23.335445851739046	4.815123109374852
8	70.08910413454535	2.18669302585155	21.827911728260766	5.8962911113423315
9	64.26895827618499	4.926030325674666	24.576235113839516	6.228776284300835
10	28.361741976371324	21.861964263755805	33.68883549787645	16.087458261996424
11	18.125644396939055	20.766371229391122	38.10265893547991	23.00532543818992
12	15.506436875112325	16.877997332551388	45.64269431228067	21.97287148005562
13	20.401961804406017	18.17506786859505	46.76548207985319	14.657488247145736
14	18.835545171634237	20.033295812484038	44.39622962759769	16.734929388284037
15	13.960120697320255	20.05315979152281	45.93568800310256	20.051031508054372
16	12.993643526707594	19.477104399398403	43.795817213556695	23.73343486033731
17	12.996717713939784	19.66723105591237	42.02201118058249	25.314040049565357
18	13.899346380499248	18.049026192075218	46.150171681533116	21.901455745892413
19	14.971291820770155	20.76471589780456	46.2965502889736	17.96744199245169
20	14.825386165211551	23.859949488739016	43.725347383157235	17.589316962892195
21	13.663106915502416	24.844162354922013	43.83696402727986	17.655766702295708
22	12.292965313708985	23.2637936416349	41.2832603410929	23.159980703563217
23	11.693025851549864	24.01507770599419	39.04383318041222	25.24806326204372
24	12.983238585306331	23.05049234290903	39.589619652096594	24.37664941968804
25	11.664175786755456	25.440318202026123	37.722169146511035	25.173336864707384
26	11.398376829141403	26.33561611441652	40.53268570455642	21.73332135188566
27	13.383119401432097	25.03097834826285	41.41970695901399	20.166195291291064
28	11.965446135510174	21.623596515290537	42.10643309149727	24.30452425770202
29	13.951607563446494	19.768679234574673	41.49088621723626	24.788826984742574
30	20.39250276676851	22.026551518648493	36.45016506020677	21.130780654376224
31	19.155024167841166	26.8889698162109	32.60482978461771	21.351176231330225
32	16.481427179598747	26.1532931639535	33.73352945071368	23.63175020573407
33	20.996698795864507	21.67987778923372	31.12732810563853	26.196095309263235
34	21.57346361581173	18.964188083504386	33.500600648889986	25.961747651793903
35	18.082369299747445	18.810478721894835	37.53417077346551	25.572981204892216
36	21.380026296124633	21.266517844474503	33.98561280375335	23.36784305564752
37	24.33313784655549	21.751766475278806	32.201638305318816	21.713457372846886
38	24.529176401592903	21.29631381303266	34.542750120602726	19.631759664771707
39	28.32106811453003	19.64642117310985	33.15037978036114	18.88213093199898
40	30.4202650422346	17.885384840946283	33.09835507335484	18.595995043464278
41	23.467872378664197	20.473850490451103	34.632138026277204	21.426139104607497
42	20.634654130288784	23.9230885649694	35.61682384434208	19.82543346039974
43	20.802079096472724	22.290695144675983	36.5686395066166	20.3385862522347
44	22.63003811992168	18.694605510835327	33.96669472847832	24.708661640764667
45	27.641909212156758	15.627985508754339	32.91366736348244	23.816437915606468
46	28.383261286996657	17.337233609852536	35.47895837077536	18.800546732375448
47	21.214020185586318	22.592201969371636	38.069552303748615	18.12422554129343
48	19.186948419867765	22.725574400060538	39.35077895174945	18.73669822832225
49	24.621165542617693	18.004568715178916	38.10360483924365	19.270660902959733
50	23.65587075170972	15.446135510173194	34.359717742316896	26.538275995800188
51	20.794038914480844	15.234016591152017	31.965635316262926	32.00630917810422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	470.0
1	844.0
2	1218.0
3	8760.0
4	16302.0
5	10684.5
6	5067.0
7	4894.5
8	4722.0
9	4891.5
10	5061.0
11	5062.0
12	5063.0
13	4811.0
14	4559.0
15	4376.5
16	4194.0
17	3974.5
18	3755.0
19	3581.0
20	3407.0
21	3195.0
22	2983.0
23	2842.5
24	2702.0
25	2741.0
26	2918.5
27	3057.0
28	3184.0
29	3311.0
30	3660.0
31	4009.0
32	4628.5
33	5248.0
34	5973.5
35	6699.0
36	7506.0
37	8313.0
38	9222.0
39	10131.0
40	12557.5
41	14984.0
42	18544.5
43	22105.0
44	26650.5
45	31196.0
46	40137.5
47	49079.0
48	57087.0
49	65095.0
50	61624.0
51	58153.0
52	46814.0
53	35475.0
54	26756.0
55	18037.0
56	13578.0
57	9119.0
58	7474.5
59	5830.0
60	4825.0
61	3820.0
62	3299.0
63	2778.0
64	2258.5
65	1739.0
66	1404.0
67	1069.0
68	813.5
69	558.0
70	447.0
71	336.0
72	268.0
73	200.0
74	149.5
75	77.0
76	55.0
77	53.0
78	51.0
79	36.0
80	21.0
81	19.5
82	18.0
83	11.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.273420056512165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76722663255659	35.609963176373356
2	11.76099728468706	11.354871244143949
3	5.211273776652167	7.5469802414944995
4	2.9597971339663123	5.715181213200665
5	1.777328968271745	4.289887393199467
6	1.1426658213303384	3.3096232306379347
7	0.7840459989316371	2.649400729503823
8	0.4915442203612662	1.8982816500680149
9	0.3843462906169541	1.6698338940703075
>10	1.566920431458358	13.109354639091709
>50	0.09517216072229107	3.151251502267828
>100	0.05375008040788164	4.545962376399066
>500	0.0019724800149681335	0.6239182797581794
>1k	0.0024656000187101667	2.1102768218451966
>5k	0.0	0.0
>10k+	4.931200037420334E-4	2.4152136079460087
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10146	2.399284896754604	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	2534	0.5992300343363066	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1886	0.44599362460863234	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1839	0.4348792553845572	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1327	0.31380357362441946	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC	1279	0.3024527284594065	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	951	0.2248886198318183	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	638	0.15087165031829664	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	523	0.12367691711045317	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	509	0.1203662539373244	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG	446	0.10546826965824496	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.72951881875538E-4	0.0	0.0	0.46562112770646713	0.0
2	4.72951881875538E-4	0.0	0.0	1.5385124717411252	0.0
3	4.72951881875538E-4	0.0	0.0	2.258108760014756	0.0
4	4.72951881875538E-4	0.0	0.0	3.3224869701756545	0.0
5	4.72951881875538E-4	0.0	0.0	5.485768877874365	0.0
6	4.72951881875538E-4	0.0	0.0	7.185794417275987	0.0
7	4.72951881875538E-4	0.0	0.0	8.801161569821886	0.0
8	4.72951881875538E-4	0.0	0.0	11.42935517740425	0.0
9	4.72951881875538E-4	0.0	0.0	12.69402850953944	0.0
10	4.72951881875538E-4	0.0	0.0	15.63318797945497	0.0
11	4.72951881875538E-4	0.0	0.0	22.626490980807613	0.0
12	4.72951881875538E-4	0.0	0.0	28.618318372288805	0.0
13	4.72951881875538E-4	0.0	0.0	30.637349956015473	0.0
14	4.72951881875538E-4	0.0	0.0	31.330224462963137	0.0
15	4.72951881875538E-4	0.0	0.0	32.276128226714214	0.0
16	4.72951881875538E-4	0.0	0.0	34.778516633717686	0.0
17	4.72951881875538E-4	0.0	0.0	38.24998344668413	0.0
18	4.72951881875538E-4	0.0	0.0	41.46700214720154	0.0
19	4.72951881875538E-4	0.0	0.0	43.38647735979342	0.0
20	4.72951881875538E-4	0.0	0.0	44.95218456474238	0.0
21	4.72951881875538E-4	0.0	0.0	46.899327462423976	0.0
22	4.72951881875538E-4	0.0	0.0	48.505945005155176	0.0
23	4.72951881875538E-4	0.0	0.0	49.74295065220065	0.0
24	4.72951881875538E-4	0.0	0.0	50.431805068152364	0.0
25	4.72951881875538E-4	0.0	0.0	50.9116147523151	0.0
26	4.72951881875538E-4	0.0	0.0	51.25024829973798	0.0
27	9.45903763751076E-4	0.0	0.0	51.534492380745185	0.0
28	9.45903763751076E-4	0.0	0.0	51.80029133835924	0.0
29	9.45903763751076E-4	0.0	0.0	52.07673171331549	0.0
30	9.45903763751076E-4	0.0	0.0	52.3297609701189	0.0
31	0.001891807527502152	0.0	0.0	52.556068445596345	0.0
32	0.001891807527502152	0.0	0.0	52.747850433696875	0.0
33	0.001891807527502152	0.0	0.0	52.92615329316396	0.0
34	0.001891807527502152	0.0	0.0	53.097834826284775	0.0
35	0.001891807527502152	0.0	0.0	53.291508621912804	0.0
36	0.00236475940937769	0.0	0.0	53.47004795732082	0.0
37	0.00236475940937769	0.0	0.0	53.65591804689791	0.0
38	0.00236475940937769	0.0	0.0	53.84580822747094	0.0
39	0.00236475940937769	0.0	0.0	54.06242018936993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	20	7.028224E-4	45.0	1
TCGTTAG	25	3.88661E-5	45.0	1
TTTACGG	35	1.2095006E-7	45.0	2
ACGTTGT	20	7.028224E-4	45.0	10
CGTTGTC	20	7.028224E-4	45.0	11
TTCCGGA	20	7.028224E-4	45.0	3
TACGGGA	45	3.8380676E-10	45.0	4
CGTTAGG	50	2.1827873E-11	45.0	2
TTGCGGA	25	3.88661E-5	45.0	3
TGGGACG	25	3.88661E-5	45.0	6
CCGTTTG	25	3.88661E-5	45.0	1
AGTCGCG	20	7.028224E-4	45.0	1
TGGTACG	20	7.028224E-4	45.0	6
AGGGTGC	30	2.1622345E-6	44.999996	6
TGTTGCG	30	2.1622345E-6	44.999996	1
CTACGGG	30	2.1622345E-6	44.999996	3
CGTTTTT	5585	0.0	44.07341	1
TCGGGAC	95	0.0	42.63158	5
TTCGTTG	75	0.0	42.0	1
TAGTTGG	65	0.0	41.53846	2
>>END_MODULE