FastQCFastQC Report
Sat 14 Jan 2017
SRR2933714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409183
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567094.024246673427085No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC33030.23439113301820985No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC19500.13837805309885232No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA14230.10098049721008555No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC207.03383E-445.017
TAGTGCG207.03383E-445.01
CTCCGCG253.8912574E-544.9999961
CGTTTTT429900.044.4766241
TATCCGG603.6379788E-1241.2499962
CTATCGG501.0822987E-940.4999962
GTTTTTT480200.040.2488562
GCGTTCG900.040.01
TGTCGCG451.9288564E-840.01
CTTCGCG850.039.7058831
GCACGCG356.2492945E-638.5714261
TACGGGA1600.037.968754
TGGGCGA21600.037.81256
TAGCGCG301.1400531E-437.4999961
TCTCGCG1200.037.4999961
AGTGCGC1300.036.34615313
AAGTGCG1300.036.34615312
CGTGCGG2750.036.02
CTATCCG250.002107270335.9999961
GCGATAT250.002107270335.9999969
Produced by FastQC (version 0.11.5)