##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1409183
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.518611848141795	34.0	31.0	34.0	31.0	34.0
2	32.71360426573412	34.0	31.0	34.0	31.0	34.0
3	32.74327606847372	34.0	31.0	34.0	31.0	34.0
4	36.23686206830482	37.0	37.0	37.0	35.0	37.0
5	36.25225609448879	37.0	37.0	37.0	35.0	37.0
6	36.225076515967054	37.0	37.0	37.0	35.0	37.0
7	36.30889103828247	37.0	37.0	37.0	35.0	37.0
8	36.21955913461914	37.0	37.0	37.0	35.0	37.0
9	38.058422504387295	39.0	39.0	39.0	37.0	39.0
10	37.49444962080865	39.0	37.0	39.0	34.0	39.0
11	37.423147313017544	39.0	37.0	39.0	34.0	39.0
12	37.49342633284677	39.0	37.0	39.0	35.0	39.0
13	37.541058187616514	39.0	37.0	39.0	35.0	39.0
14	38.910384243920056	40.0	38.0	41.0	35.0	41.0
15	38.94751923632346	40.0	38.0	41.0	35.0	41.0
16	38.951743669913704	40.0	38.0	41.0	35.0	41.0
17	38.86947614326883	40.0	38.0	41.0	35.0	41.0
18	38.33814557796965	39.0	38.0	40.0	35.0	41.0
19	37.77665214525012	38.0	37.0	40.0	34.0	41.0
20	37.16838976910735	38.0	35.0	40.0	34.0	41.0
21	37.057871830699064	38.0	35.0	40.0	33.0	41.0
22	37.062182129645336	38.0	35.0	40.0	33.0	41.0
23	36.960990162384874	38.0	35.0	40.0	33.0	41.0
24	36.87164974314904	38.0	35.0	40.0	33.0	41.0
25	36.81258431303812	38.0	35.0	40.0	33.0	41.0
26	36.77645131966537	38.0	35.0	40.0	33.0	41.0
27	36.68950306667055	38.0	35.0	40.0	33.0	41.0
28	36.531026133582365	37.0	35.0	40.0	33.0	41.0
29	36.37053739649144	37.0	35.0	40.0	33.0	41.0
30	36.09837828018079	38.0	35.0	40.0	31.0	41.0
31	35.562682064714096	38.0	35.0	40.0	30.0	41.0
32	34.64808190277629	37.0	35.0	40.0	21.0	41.0
33	33.500964033769925	37.0	33.0	40.0	15.0	41.0
34	32.45250120105054	37.0	31.0	40.0	10.0	41.0
35	31.773284236327008	37.0	29.0	40.0	7.0	41.0
36	31.411382339979973	37.0	27.0	40.0	7.0	41.0
37	31.175971467155083	37.0	25.0	40.0	7.0	41.0
38	31.01412733477483	37.0	24.0	40.0	7.0	41.0
39	30.907316508927515	37.0	23.0	40.0	7.0	41.0
40	30.75573222214574	37.0	23.0	40.0	7.0	41.0
41	30.600547977090272	37.0	23.0	40.0	7.0	41.0
42	30.452790730515485	36.0	22.0	40.0	7.0	41.0
43	30.292930016896314	36.0	21.0	40.0	7.0	41.0
44	30.104341309822782	36.0	21.0	40.0	7.0	41.0
45	30.024520591009118	36.0	20.0	40.0	7.0	41.0
46	29.826564044556314	35.0	20.0	40.0	7.0	41.0
47	29.699689820271747	35.0	20.0	40.0	7.0	41.0
48	29.606489008169984	35.0	20.0	40.0	7.0	41.0
49	29.547397321710523	35.0	20.0	40.0	7.0	41.0
50	29.419748889959642	35.0	20.0	40.0	7.0	41.0
51	28.572317434996023	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	9.0
12	6.0
13	12.0
14	14.0
15	54.0
16	78.0
17	223.0
18	579.0
19	1271.0
20	2256.0
21	3614.0
22	5904.0
23	10429.0
24	21486.0
25	46911.0
26	77680.0
27	82073.0
28	65296.0
29	49167.0
30	40077.0
31	36091.0
32	37161.0
33	42240.0
34	59000.0
35	76257.0
36	97373.0
37	132006.0
38	237571.0
39	284263.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.379184250732514	3.2195250723291444	35.97077171666135	40.430518960276984
2	44.15352725657349	3.5886751401343897	37.1012849289269	15.156512674365215
3	15.271827718614261	3.962863588334517	66.5956089450412	14.169699748010018
4	12.227226698022896	3.596623007799555	67.78679561135779	16.38935468281976
5	12.585306521580234	4.362456827821511	67.32780625369452	15.724430396903738
6	14.705542147471265	5.277313166565308	68.08789206228005	11.929252623683368
7	60.038831010592666	1.2136110072290114	36.09098321509697	2.6565747670813513
8	61.811347426132734	1.593121688240633	34.29064926272883	2.304881622897807
9	57.400635687487004	3.818453671382638	35.65683094388735	3.1240796972430123
10	23.427759205156462	23.29853539249338	42.04712943599234	11.226575966357812
11	18.36745121109182	19.135910665967444	48.81175830250577	13.684879820434961
12	14.434746942022434	19.20559643424594	51.51992324630654	14.839733377425077
13	15.302980521337542	18.41847368297801	53.14767492937397	13.13087086631048
14	15.068660351423485	19.647909462433198	52.08244777292942	13.200982413213897
15	14.254926436098078	18.7471747814159	52.455429848358946	14.54246893412708
16	15.293471465381003	19.42075656603862	51.349611796338735	13.936160172241646
17	15.26764089546922	19.096739032474847	51.31725262084485	14.318367451211092
18	16.920584480511046	17.17399372544233	51.18696436161946	14.71845743242716
19	15.962866426858682	19.744135431665015	49.84554880381044	14.44744933766587
20	17.728712310608348	19.79239034248923	49.055516565272214	13.423380781630206
21	16.85331145777376	21.5562492593226	48.97951508072408	12.610924202179561
22	16.939673555528273	18.564302862012955	49.32155724274278	15.174466339715991
23	14.83873989396693	21.396369385665313	48.75371048330842	15.011180237059346
24	14.215612876397174	20.658211176263126	50.07404999918392	15.052125948155776
25	14.68624018314158	22.340817338841017	48.38761182898176	14.585330649035647
26	15.635726516712165	21.353791523173356	48.59929476867092	14.411187191443553
27	15.65190610445911	20.768771692533903	48.885630893929324	14.693691309077671
28	14.167003150052192	21.59811749077302	48.779966831845115	15.454912527329665
29	15.85365420956682	20.19929278170401	48.23851834715576	15.70853466157341
30	17.26503938807096	19.692829107362208	48.03393171788192	15.008199786684909
31	17.482683228509003	21.468964641214093	45.50090371513139	15.547448415145512
32	16.815771975676686	21.524741641078553	45.20498757081231	16.45449881243245
33	17.01425577799335	22.862609043679917	41.643633225776924	18.47950195254981
34	17.153982130071114	24.915145868208743	40.057891700368224	17.87298030135192
35	17.8900114463487	26.072341207635912	37.692265660315236	18.345381685700154
36	18.939200941254615	28.042986609971877	35.07443674810156	17.94337570067195
37	18.279173109525164	27.784397058437406	34.843735696499316	19.09269413553811
38	17.758587777456867	28.46975871834957	35.5110017648524	18.260651739341164
39	18.463890069636093	27.34250980887507	34.44449727253309	19.749102848955744
40	19.926865424859653	27.180501042093187	35.02767206246457	17.8649614705826
41	18.6774890131374	27.116279432834485	34.78150105415691	19.424730499871202
42	20.10633111526324	26.72697584344971	34.56435395544795	18.602339085839102
43	19.42622072505842	26.244710587624176	34.71855678077297	19.61051190654443
44	19.68871324732132	27.059154133991115	33.93640144679576	19.315731171891798
45	20.348883005259076	25.723202735201887	33.74735573733149	20.180558522207548
46	19.763579322203007	26.657219112067065	33.78674026013655	19.792461305593385
47	18.458922652345365	27.50735709982309	34.90667996988326	19.127040277948286
48	19.160960641733542	26.597681067682476	34.87318538472292	19.368172905861055
49	20.012021149843562	25.595753000142636	34.49835826858541	19.89386758142839
50	18.673373153096513	25.842775565700126	34.63084638403955	20.853004897163817
51	18.477231133216907	24.996043806943455	33.877289181036105	22.649435878803533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	1234.0
2	2103.0
3	49077.5
4	96052.0
5	66186.5
6	36321.0
7	35627.0
8	34933.0
9	35369.5
10	35806.0
11	35774.5
12	35743.0
13	34753.5
14	33764.0
15	32523.5
16	31283.0
17	29989.0
18	28695.0
19	27292.0
20	25889.0
21	24626.0
22	23363.0
23	22622.0
24	21881.0
25	21325.0
26	20988.5
27	21208.0
28	22014.0
29	22820.0
30	24426.5
31	26033.0
32	28385.5
33	30738.0
34	33635.5
35	36533.0
36	40125.0
37	43717.0
38	47187.5
39	50658.0
40	53224.0
41	55790.0
42	60911.5
43	66033.0
44	69785.0
45	73537.0
46	81609.5
47	89682.0
48	94174.5
49	98667.0
50	96807.0
51	94947.0
52	86595.0
53	78243.0
54	68323.5
55	58404.0
56	50520.0
57	42636.0
58	36721.5
59	30807.0
60	26359.0
61	21911.0
62	18414.0
63	14917.0
64	11828.0
65	8739.0
66	6878.0
67	5017.0
68	4040.0
69	3063.0
70	2397.5
71	1732.0
72	1679.5
73	1627.0
74	1835.0
75	1815.0
76	1587.0
77	1122.0
78	657.0
79	478.0
80	299.0
81	205.5
82	112.0
83	73.5
84	35.0
85	26.5
86	18.0
87	11.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1409183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.89330385390161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98990516014017	34.5633120603724
2	8.294948498496288	7.447752867909176
3	3.8754764621213136	5.219488271779672
4	2.456107805234562	4.410511759933383
5	1.6744943900712659	3.758679272756148
6	1.263447019879232	3.4032186580046893
7	0.9274199507661113	2.914446195493945
8	0.7611384681161715	2.733601641922599
9	0.6070117880808324	2.4525688180691647
>10	3.025806412452807	21.10587376465293
>50	0.07501060438430932	2.309740840347074
>100	0.04400933176885821	3.5767659806027465
>500	0.0036410453099205275	1.1145741276798236
>1k	0.0014247568604036846	0.9592174329716622
>5k	0.0	0.0
>10k+	1.5830631782263164E-4	4.03024830750463
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56709	4.024246673427085	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC	3303	0.23439113301820985	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	1950	0.13837805309885232	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1423	0.10098049721008555	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1288931245977278E-4	0.0	0.0	0.09963219823117367	0.0
2	2.1288931245977278E-4	0.0	0.0	0.31472136691969743	0.0
3	2.1288931245977278E-4	0.0	0.0	0.5115020547366808	0.0
4	2.1288931245977278E-4	0.0	0.0	0.7419192539223082	0.0
5	2.1288931245977278E-4	0.0	0.0	1.2862772258819473	0.0
6	2.1288931245977278E-4	0.0	0.0	2.0571494262987846	0.0
7	2.1288931245977278E-4	0.0	0.0	2.763019423311238	0.0
8	2.1288931245977278E-4	0.0	0.0	3.8067447591973504	0.0
9	2.1288931245977278E-4	1.419262083065152E-4	0.0	4.492957976359351	0.0
10	2.1288931245977278E-4	1.419262083065152E-4	0.0	5.530367595975824	0.0
11	2.1288931245977278E-4	1.419262083065152E-4	0.0	6.828282770938905	0.0
12	2.1288931245977278E-4	1.419262083065152E-4	0.0	7.906992917172574	0.0
13	2.1288931245977278E-4	1.419262083065152E-4	0.0	8.386419648831982	0.0
14	2.1288931245977278E-4	1.419262083065152E-4	0.0	8.610237279331358	0.0
15	3.5481552076628797E-4	1.419262083065152E-4	0.0	8.838880400913153	0.0
16	4.2577862491954556E-4	1.419262083065152E-4	0.0	9.235422226921557	0.0
17	4.2577862491954556E-4	1.419262083065152E-4	0.0	9.742027827471663	0.0
18	4.2577862491954556E-4	1.419262083065152E-4	0.0	10.260626192623668	0.0
19	4.2577862491954556E-4	1.419262083065152E-4	0.0	10.585566246541436	0.0
20	4.2577862491954556E-4	1.419262083065152E-4	0.0	10.88495958296403	0.0
21	4.2577862491954556E-4	1.419262083065152E-4	0.0	11.199397097467115	0.0
22	4.2577862491954556E-4	1.419262083065152E-4	0.0	11.50035162218108	0.0
23	4.2577862491954556E-4	1.419262083065152E-4	0.0	11.771856458671444	0.0
24	4.2577862491954556E-4	1.419262083065152E-4	0.0	11.985810217693514	0.0
25	4.2577862491954556E-4	1.419262083065152E-4	0.0	12.1608761956396	0.0
26	4.2577862491954556E-4	1.419262083065152E-4	0.0	12.333174612523711	0.0
27	4.2577862491954556E-4	1.419262083065152E-4	0.0	12.504976287678748	0.0
28	4.967417290728032E-4	1.419262083065152E-4	0.0	12.69018998951875	0.0
29	4.967417290728032E-4	1.419262083065152E-4	0.0	12.883919263857143	0.0
30	4.967417290728032E-4	1.419262083065152E-4	0.0	13.134560947726449	0.0
31	4.967417290728032E-4	1.419262083065152E-4	0.0	13.313317007088504	0.0
32	4.967417290728032E-4	1.419262083065152E-4	0.0	13.530535068901626	0.0
33	5.677048332260608E-4	1.419262083065152E-4	0.0	13.718658258011912	0.0
34	5.677048332260608E-4	1.419262083065152E-4	0.0	13.897059501853201	0.0
35	5.677048332260608E-4	1.419262083065152E-4	0.0	14.104342729084866	0.0
36	5.677048332260608E-4	1.419262083065152E-4	0.0	14.272454322823934	0.0
37	5.677048332260608E-4	1.419262083065152E-4	0.0	14.452700607373208	0.0
38	5.677048332260608E-4	1.419262083065152E-4	0.0	14.655371232834913	0.0
39	5.677048332260608E-4	1.419262083065152E-4	0.0	14.890755849311267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCC	20	7.03383E-4	45.0	17
TAGTGCG	20	7.03383E-4	45.0	1
CTCCGCG	25	3.8912574E-5	44.999996	1
CGTTTTT	42990	0.0	44.476624	1
TATCCGG	60	3.6379788E-12	41.249996	2
CTATCGG	50	1.0822987E-9	40.499996	2
GTTTTTT	48020	0.0	40.248856	2
GCGTTCG	90	0.0	40.0	1
TGTCGCG	45	1.9288564E-8	40.0	1
CTTCGCG	85	0.0	39.705883	1
GCACGCG	35	6.2492945E-6	38.571426	1
TACGGGA	160	0.0	37.96875	4
TGGGCGA	2160	0.0	37.8125	6
TAGCGCG	30	1.1400531E-4	37.499996	1
TCTCGCG	120	0.0	37.499996	1
AGTGCGC	130	0.0	36.346153	13
AAGTGCG	130	0.0	36.346153	12
CGTGCGG	275	0.0	36.0	2
CTATCCG	25	0.0021072703	35.999996	1
GCGATAT	25	0.0021072703	35.999996	9
>>END_MODULE