FastQCFastQC Report
Sat 14 Jan 2017
SRR2933713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421190
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559883.9395154764669047No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC31950.22481160154518395No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC18530.1303836925393508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT431300.044.415721
CGTATGG556.184564E-1140.9090922
GTTTTTT487550.039.7713052
GGATCGA850.039.7058838
CGTGCGG2100.039.642862
AACACGC700.038.57142641
TCTTGCG3100.038.467741
TACGGGA1700.038.3823554
TGGGCGA22300.038.0381166
CAGCGCG301.140058E-437.4999961
CCGTGCG301.140058E-437.4999961
GCGTTAG601.5643309E-1037.4999961
TGCGGGA14900.037.4496654
TAGGGCG1750.037.2857135
ACACGAC8350.037.18562726
GACACGA8350.037.18562725
GCTTTCG2800.036.9642831
AACACGT7500.036.941
GGTCGTT552.750312E-936.818188
TCAAGCG9350.036.8181817
Produced by FastQC (version 0.11.5)