##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933713.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421190
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.440067126844404	34.0	31.0	34.0	31.0	34.0
2	32.65307735067092	34.0	31.0	34.0	31.0	34.0
3	32.6966506941366	34.0	31.0	34.0	31.0	34.0
4	36.16302042654395	37.0	37.0	37.0	35.0	37.0
5	36.22573125338624	37.0	35.0	37.0	35.0	37.0
6	36.215589752249876	37.0	37.0	37.0	35.0	37.0
7	36.23869222271477	37.0	37.0	37.0	35.0	37.0
8	36.185013263532674	37.0	37.0	37.0	35.0	37.0
9	38.027345393648986	39.0	39.0	39.0	35.0	39.0
10	37.506093485037184	39.0	37.0	39.0	34.0	39.0
11	37.43170371308551	39.0	37.0	39.0	34.0	39.0
12	37.476015874021066	39.0	37.0	39.0	35.0	39.0
13	37.5376114383017	39.0	37.0	39.0	35.0	39.0
14	38.93468361021397	40.0	38.0	41.0	35.0	41.0
15	38.95316389786024	40.0	38.0	41.0	35.0	41.0
16	39.00597034879221	40.0	38.0	41.0	35.0	41.0
17	38.94563358875309	40.0	38.0	41.0	35.0	41.0
18	38.456251451248605	39.0	38.0	41.0	35.0	41.0
19	37.92434157290721	38.0	37.0	41.0	35.0	41.0
20	37.27043041394887	38.0	35.0	41.0	34.0	41.0
21	37.20004643995525	38.0	35.0	40.0	34.0	41.0
22	37.183658764837915	38.0	35.0	40.0	34.0	41.0
23	37.03050893969139	38.0	35.0	40.0	33.0	41.0
24	36.937578367424486	38.0	35.0	40.0	33.0	41.0
25	36.890037925963455	38.0	35.0	40.0	33.0	41.0
26	36.87598632132227	38.0	35.0	40.0	33.0	41.0
27	36.800859139172104	38.0	35.0	40.0	33.0	41.0
28	36.70189559453697	38.0	35.0	40.0	33.0	41.0
29	36.493828411401715	38.0	35.0	40.0	33.0	41.0
30	36.13556315482096	38.0	35.0	40.0	31.0	41.0
31	35.60193147995694	38.0	35.0	40.0	30.0	41.0
32	34.772955762424445	38.0	35.0	40.0	21.0	41.0
33	33.56091937038679	38.0	33.0	40.0	15.0	41.0
34	32.544201690132915	38.0	31.0	40.0	10.0	41.0
35	31.929979805655822	38.0	30.0	40.0	7.0	41.0
36	31.55774456617342	37.0	27.0	40.0	7.0	41.0
37	31.355089045096012	37.0	25.0	40.0	7.0	41.0
38	31.177359818180538	37.0	24.0	40.0	7.0	41.0
39	31.097002511979397	37.0	24.0	40.0	7.0	41.0
40	30.94226387745481	37.0	23.0	40.0	7.0	41.0
41	30.796948332031608	37.0	23.0	40.0	7.0	41.0
42	30.589615040916414	37.0	22.0	40.0	7.0	41.0
43	30.4541862805114	36.0	22.0	40.0	7.0	41.0
44	30.287022143415026	36.0	21.0	40.0	7.0	41.0
45	30.186363540413314	36.0	21.0	40.0	7.0	41.0
46	29.977551910722703	35.0	20.0	40.0	7.0	41.0
47	29.91533714703875	35.0	20.0	40.0	7.0	41.0
48	29.786848345400685	35.0	20.0	40.0	7.0	41.0
49	29.716078779051358	35.0	20.0	40.0	7.0	41.0
50	29.518564020292853	35.0	20.0	40.0	7.0	41.0
51	28.781027871009506	35.0	19.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	8.0
11	11.0
12	10.0
13	7.0
14	23.0
15	46.0
16	93.0
17	233.0
18	571.0
19	1223.0
20	2254.0
21	3627.0
22	5907.0
23	10470.0
24	21237.0
25	46432.0
26	77323.0
27	81198.0
28	65156.0
29	49829.0
30	40067.0
31	36482.0
32	36499.0
33	41250.0
34	58541.0
35	74115.0
36	94928.0
37	126657.0
38	236680.0
39	310156.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.612303773598182	3.371540751060731	35.75693608877068	40.25921938657041
2	43.99890232833049	3.6497583011420005	37.00469325002287	15.346646120504648
3	15.395970982064327	4.045201556442136	66.34996024458376	14.208867216909773
4	12.451114910743813	3.6912024430231005	67.44925027617701	16.40843237005608
5	12.726166100239938	4.395612127864677	67.17743581083458	15.7007859610608
6	14.79724737719798	5.338554310120392	67.80648611374974	12.057712198931881
7	60.12123642862671	1.2761136793813637	35.86705507356511	2.735594818426811
8	61.89102090501621	1.624835525158494	34.12562711530478	2.3585164545205073
9	57.466630077611015	3.8806915331517957	35.48153308143176	3.1711453078054306
10	23.51494170378345	23.389624188180328	41.88187364110358	11.213560466932641
11	18.444050408460516	19.208691307988378	48.61869278562331	13.728565497927791
12	14.447118260049677	19.30192303632871	51.39214320393472	14.85881549968688
13	15.4042035195857	18.482468916893588	52.962235872754526	13.15109169076619
14	15.137807049022298	19.72403408411261	51.87765182699005	13.260507039875034
15	14.296680950471083	18.83949366376065	52.287167796001945	14.576657589766324
16	15.389919715168274	19.49971502754734	51.185766857351936	13.92459839993245
17	15.357693200768372	19.223819475228506	51.10484875350938	14.313638570493742
18	17.066542826786	17.24083338610601	50.97045433756219	14.722169449545802
19	16.048733807583783	19.757386415609453	49.68315285077998	14.510726926026779
20	17.85595170244654	19.80009710172461	48.885441074029515	13.458510121799335
21	16.93397786362133	21.66205785292607	48.74182903060111	12.662135252851483
22	17.06175810412401	18.560502114425233	49.1883562366749	15.189383544775856
23	14.972945207889163	21.541736150690618	48.452071855276216	15.033246786144005
24	14.314060751905094	20.744165101077268	49.93350642771199	15.008267719305652
25	14.794292107318515	22.44133437471415	48.13015852911997	14.634214988847374
26	15.731394113383853	21.44484551678523	48.44644276979152	14.377317600039405
27	15.808794038798471	20.794545416165327	48.68103490736636	14.715625637669842
28	14.254603536472956	21.750153040761617	48.59885025928975	15.39639316347568
29	15.916591025830465	20.296582441475103	48.08568875379084	15.701137778903595
30	17.364180721789484	19.77448476276923	47.90098438632413	14.960350129117147
31	17.54198945953743	21.49916619171258	45.371414096637324	15.587430252112666
32	16.879938642968217	21.64749259423441	45.14772831218908	16.324840450608296
33	17.054721747268133	22.892364849175692	41.57002230525123	18.48289109830494
34	17.134514034013748	25.01678171110126	39.98860110189349	17.860103152991506
35	17.907809652474334	26.004686213666012	37.71022875196138	18.37727538189827
36	18.947923922909673	28.067816407376917	35.05970348792209	17.924556181791313
37	18.21192099578522	27.758427796424122	34.8980080073741	19.13164320041655
38	17.797057395562874	28.489997818729375	35.54450847529183	18.16843631041592
39	18.421111885110363	27.349896917372064	34.499750209331616	19.729240988185957
40	19.901139186174966	27.18376853200487	35.050204406166664	17.8648878756535
41	18.608138250339504	27.20445542116114	34.83496224994547	19.352444078553887
42	20.109978257657314	26.77608201577551	34.52810672746079	18.585832999106383
43	19.42850709616589	26.265383235176152	34.8251817139158	19.480927954742153
44	19.77568094343473	27.130151492763105	33.88660207291073	19.207565490891437
45	20.3179026027484	25.825329477409774	33.84642447526369	20.010343444578137
46	19.759567686234774	26.73147151330927	33.809061420358994	19.699899380096962
47	18.49633053989966	27.632406645135415	34.97477466067169	18.896488154293234
48	19.225086019462562	26.754410036659422	34.787326114031195	19.233177829846817
49	20.08619537148446	25.71809539892625	34.43944863107677	19.756260598512515
50	18.663232924521	25.957824077005892	34.60578810714964	20.77315489132347
51	18.509066345808794	25.10227344690013	33.88104335099459	22.507616856296483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	1244.5
2	2100.0
3	49157.5
4	96215.0
5	66632.0
6	37049.0
7	36200.5
8	35352.0
9	35701.5
10	36051.0
11	36067.0
12	36083.0
13	35124.5
14	34166.0
15	32829.0
16	31492.0
17	30125.0
18	28758.0
19	27084.0
20	25410.0
21	24376.0
22	23342.0
23	22348.0
24	21354.0
25	20965.5
26	20855.5
27	21134.0
28	22011.5
29	22889.0
30	24700.5
31	26512.0
32	28708.0
33	30904.0
34	34150.5
35	37397.0
36	41103.5
37	44810.0
38	47895.5
39	50981.0
40	53587.5
41	56194.0
42	61255.5
43	66317.0
44	70714.5
45	75112.0
46	83228.0
47	91344.0
48	95316.0
49	99288.0
50	97445.0
51	95602.0
52	86731.5
53	77861.0
54	68345.0
55	58829.0
56	51185.5
57	43542.0
58	37450.5
59	31359.0
60	26833.5
61	22308.0
62	18709.5
63	15111.0
64	11945.0
65	8779.0
66	6948.0
67	5117.0
68	4150.0
69	3183.0
70	2422.5
71	1662.0
72	1670.5
73	1679.0
74	1886.0
75	1886.5
76	1680.0
77	1175.5
78	671.0
79	484.5
80	298.0
81	213.0
82	128.0
83	86.0
84	44.0
85	31.0
86	18.0
87	10.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1421190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.60130307647627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6950470303327	34.206990370644704
2	8.394064665755385	7.487724444017927
3	3.8912182633318375	5.206602152987488
4	2.4362848629223137	4.346459182073183
5	1.7237831026790187	3.8441486300347756
6	1.2899511082061372	3.452010019856301
7	0.995673715203346	3.108584159594589
8	0.7800600535013146	2.783335589125154
9	0.6208170478792897	2.492032437675673
>10	3.0510874080594292	21.223917706331058
>50	0.07370268455239605	2.2855667399418524
>100	0.04290697402612048	3.4896048460297058
>500	0.0038139413299312845	1.1503469183267605
>1k	0.0014302279987242318	0.9543697631096628
>5k	0.0	0.0
>10k+	1.5891422208047021E-4	3.968307040251304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55988	3.9395154764669047	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC	3195	0.22481160154518395	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	1853	0.1303836925393508	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.518178427937151E-4	0.0	0.0	0.1004088123333263	0.0
2	3.518178427937151E-4	0.0	0.0	0.3113587908724379	0.0
3	3.518178427937151E-4	0.0	0.0	0.5111209620107093	0.0
4	3.518178427937151E-4	0.0	0.0	0.7425467390004151	0.0
5	3.518178427937151E-4	0.0	0.0	1.2773802236154208	0.0
6	3.518178427937151E-4	0.0	0.0	2.0375882183240805	0.0
7	3.518178427937151E-4	0.0	0.0	2.7194111976583004	0.0
8	3.518178427937151E-4	0.0	0.0	3.7465082079102725	0.0
9	4.2218141135245816E-4	1.4072713711748606E-4	0.0	4.419395014037532	0.0
10	4.2218141135245816E-4	1.4072713711748606E-4	0.0	5.433967309086047	0.0
11	4.2218141135245816E-4	1.4072713711748606E-4	0.0	6.701918814514597	0.0
12	4.2218141135245816E-4	1.4072713711748606E-4	0.0	7.757161252190066	0.0
13	4.925449799112012E-4	1.4072713711748606E-4	0.0	8.225290073811383	0.0
14	4.925449799112012E-4	1.4072713711748606E-4	0.0	8.442924591363576	0.0
15	4.925449799112012E-4	1.4072713711748606E-4	0.0	8.662951470246766	0.0
16	4.925449799112012E-4	1.4072713711748606E-4	0.0	9.037285654979279	0.0
17	4.925449799112012E-4	1.4072713711748606E-4	0.0	9.534052449004003	0.0
18	4.925449799112012E-4	1.4072713711748606E-4	0.0	10.0257530660925	0.0
19	4.925449799112012E-4	1.4072713711748606E-4	0.0	10.340981853235668	0.0
20	5.629085484699443E-4	1.4072713711748606E-4	0.0	10.622295400333524	0.0
21	5.629085484699443E-4	1.4072713711748606E-4	0.0	10.945686361429507	0.0
22	5.629085484699443E-4	1.4072713711748606E-4	0.0	11.241213349376228	0.0
23	5.629085484699443E-4	1.4072713711748606E-4	7.036356855874303E-5	11.516545993146588	0.0
24	5.629085484699443E-4	1.4072713711748606E-4	7.036356855874303E-5	11.719896706281355	0.0
25	6.332721170286872E-4	1.4072713711748606E-4	7.036356855874303E-5	11.892428176387394	0.0
26	6.332721170286872E-4	1.4072713711748606E-4	7.036356855874303E-5	12.056516018266382	0.0
27	6.332721170286872E-4	1.4072713711748606E-4	7.036356855874303E-5	12.229962214763685	0.0
28	7.036356855874302E-4	1.4072713711748606E-4	7.036356855874303E-5	12.41093731309677	0.0
29	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	12.60134112961673	0.0
30	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	12.857112701327761	0.0
31	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	13.031543987784884	0.0
32	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	13.239609060013088	0.0
33	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	13.433319964255308	0.0
34	7.739992541461733E-4	1.4072713711748606E-4	7.036356855874303E-5	13.60838452282946	0.0
35	8.443628227049163E-4	1.4072713711748606E-4	7.036356855874303E-5	13.825667222538858	0.0
36	8.443628227049163E-4	1.4072713711748606E-4	7.036356855874303E-5	13.995102695628312	0.0
37	8.443628227049163E-4	1.4072713711748606E-4	7.036356855874303E-5	14.184169604345653	0.0
38	8.443628227049163E-4	1.4072713711748606E-4	7.036356855874303E-5	14.391249586614034	0.0
39	8.443628227049163E-4	1.4072713711748606E-4	7.036356855874303E-5	14.617609186667512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	43130	0.0	44.41572	1
CGTATGG	55	6.184564E-11	40.909092	2
GTTTTTT	48755	0.0	39.771305	2
GGATCGA	85	0.0	39.705883	8
CGTGCGG	210	0.0	39.64286	2
AACACGC	70	0.0	38.571426	41
TCTTGCG	310	0.0	38.46774	1
TACGGGA	170	0.0	38.382355	4
TGGGCGA	2230	0.0	38.038116	6
CAGCGCG	30	1.140058E-4	37.499996	1
CCGTGCG	30	1.140058E-4	37.499996	1
GCGTTAG	60	1.5643309E-10	37.499996	1
TGCGGGA	1490	0.0	37.449665	4
TAGGGCG	175	0.0	37.285713	5
ACACGAC	835	0.0	37.185627	26
GACACGA	835	0.0	37.185627	25
GCTTTCG	280	0.0	36.964283	1
AACACGT	750	0.0	36.9	41
GGTCGTT	55	2.750312E-9	36.81818	8
TCAAGCG	935	0.0	36.81818	17
>>END_MODULE