##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086752
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64570481581814	34.0	31.0	34.0	31.0	34.0
2	32.85837247136421	34.0	31.0	34.0	31.0	34.0
3	32.84868672889491	34.0	31.0	34.0	31.0	34.0
4	36.32780339948765	37.0	37.0	37.0	35.0	37.0
5	36.344031572980775	37.0	37.0	37.0	35.0	37.0
6	36.334116707399666	37.0	37.0	37.0	35.0	37.0
7	36.4050657371691	37.0	37.0	37.0	35.0	37.0
8	36.331547583993405	37.0	37.0	37.0	35.0	37.0
9	38.179773306145286	39.0	39.0	39.0	37.0	39.0
10	37.56721772768764	39.0	37.0	39.0	34.0	39.0
11	37.57813374164483	39.0	37.0	39.0	35.0	39.0
12	37.669673485763084	39.0	37.0	39.0	35.0	39.0
13	37.72402811312977	39.0	38.0	39.0	35.0	39.0
14	39.15240275610259	40.0	39.0	41.0	36.0	41.0
15	39.18478916993021	41.0	39.0	41.0	36.0	41.0
16	39.20031341097141	41.0	39.0	41.0	36.0	41.0
17	39.166590905744826	41.0	39.0	41.0	36.0	41.0
18	38.50823370925473	39.0	38.0	40.0	35.0	41.0
19	37.82743164953918	37.0	37.0	40.0	35.0	41.0
20	37.069821817673215	37.0	35.0	40.0	34.0	41.0
21	36.98155788993257	37.0	35.0	40.0	34.0	41.0
22	36.97491792055593	37.0	35.0	40.0	34.0	41.0
23	36.900072877712674	37.0	35.0	40.0	34.0	41.0
24	36.79757387149966	37.0	35.0	40.0	34.0	41.0
25	36.736461492594444	37.0	35.0	40.0	34.0	41.0
26	36.67767347104031	37.0	35.0	40.0	33.0	41.0
27	36.60527608874886	37.0	35.0	40.0	33.0	41.0
28	36.41831162951621	36.0	35.0	40.0	33.0	41.0
29	36.199621440770294	36.0	35.0	40.0	33.0	41.0
30	35.80768565413268	36.0	35.0	40.0	31.0	41.0
31	35.099619784458646	37.0	35.0	40.0	26.0	41.0
32	33.93265528841907	37.0	34.0	40.0	18.0	41.0
33	32.42159296693266	37.0	31.0	40.0	10.0	41.0
34	31.121825402667767	37.0	23.0	40.0	7.0	41.0
35	30.33130649863078	37.0	20.0	40.0	7.0	41.0
36	29.912692132151587	37.0	18.0	40.0	7.0	41.0
37	29.668494743971024	36.0	18.0	40.0	7.0	41.0
38	29.527257368746504	36.0	17.0	40.0	7.0	41.0
39	29.414074232207533	36.0	16.0	40.0	7.0	41.0
40	29.293171763198963	36.0	15.0	40.0	7.0	41.0
41	29.146244957451195	36.0	15.0	40.0	7.0	41.0
42	29.02639240599511	36.0	15.0	40.0	7.0	41.0
43	28.888064618238566	36.0	15.0	40.0	7.0	41.0
44	28.726355231000266	35.0	15.0	40.0	7.0	41.0
45	28.689411199611317	35.0	15.0	40.0	7.0	41.0
46	28.540083662141868	35.0	15.0	40.0	7.0	41.0
47	28.44197020111304	35.0	13.0	40.0	7.0	41.0
48	28.350320036218015	35.0	12.0	40.0	7.0	41.0
49	28.319164814051412	35.0	12.0	40.0	7.0	41.0
50	28.2126060039457	35.0	12.0	40.0	7.0	41.0
51	27.457080364241335	34.0	10.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	4.0
12	3.0
13	4.0
14	13.0
15	14.0
16	53.0
17	124.0
18	368.0
19	824.0
20	1542.0
21	2799.0
22	5021.0
23	10066.0
24	22520.0
25	50863.0
26	81544.0
27	78011.0
28	54906.0
29	36371.0
30	26756.0
31	23533.0
32	23030.0
33	26715.0
34	38129.0
35	49980.0
36	63838.0
37	90450.0
38	177568.0
39	221631.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.674369129295368	3.157022025264274	36.64672344748388	45.52188539795648
2	50.093305556373494	2.9063668619887517	34.98295839345131	12.01736918818645
3	12.814423161862134	2.9761159859839226	70.81873325284886	13.390727599305086
4	10.8811393951886	3.0595756897617856	71.04831645122346	15.010968463826154
5	10.389674921233178	3.951039427578693	71.20713833514914	14.452147316038985
6	12.586772327081064	4.657640381614205	72.7159462324431	10.039641058861635
7	53.93585657077236	1.0919694649745297	42.30431598009481	2.667857984158299
8	55.77629486764229	1.256680456994788	40.545773092665115	2.4212515826978005
9	51.52665925620564	3.4743897411736993	41.944068195871736	3.0548828067489175
10	22.162278054238683	14.483525220105415	50.69693913606784	12.657257589588056
11	16.766014693324696	17.506938105473928	51.62576190335974	14.101285297841642
12	12.644467182945144	15.614786078148466	57.18149126939724	14.559255469509145
13	14.266088307175878	15.945680339212625	58.24677571331822	11.541455640293277
14	14.214834663290244	16.51802803215453	57.287311180471725	11.979826124083507
15	12.857395247489766	17.239075704484556	57.539898707340775	12.363630340684903
16	12.729399163746653	16.385063013456612	57.106037071935454	13.779500750861281
17	13.22049556844616	16.843677306321958	55.61185992756397	14.323967197667914
18	13.753367833691588	15.959206884367363	56.73649553900062	13.550929742940433
19	14.1648692618003	18.373833220458764	54.99782839139012	12.463469126350814
20	14.57563455139719	17.953314095580225	54.68432540266777	12.78672595035482
21	14.481960925767792	18.86253717499485	54.47296163246076	12.182540266776597
22	13.987459880451105	17.308456759223816	54.037995789287706	14.666087571037368
23	12.648976031330056	18.79472041459321	53.47558596625541	15.080717587821324
24	12.494662995789287	18.317886693560258	54.26978740319779	14.917662907452666
25	12.681274108536261	19.228121963428638	53.45359382821472	14.637010099820383
26	12.713480168428493	19.507118459409323	54.11943111215807	13.659970260004123
27	13.171910426665882	19.016850210535615	53.97505594652689	13.836183416271606
28	12.851782191337122	18.050944465710668	54.0114948028621	15.085778540090104
29	13.596938399929332	17.260791790583315	53.30747033361798	15.83479947586938
30	14.526957392302936	17.729620005300198	52.04710918406408	15.696313418332794
31	13.738829098083094	19.195179765024587	50.363652424840254	16.702338712052057
32	13.871794116781015	18.927225346721237	48.884474102647154	18.316506433850595
33	15.596474632666881	19.234931244662995	44.968309237066045	20.200284885604074
34	15.619386943847354	20.148019051264686	42.2272974882954	22.005296516592562
35	16.38027811312977	21.033133594417126	40.5578273607962	22.0287609316569
36	16.644827890815936	22.195864373840582	38.614973793468984	22.544333941874502
37	16.94848502694267	23.874720267365507	38.03912944259592	21.137665263095904
38	18.56863387414976	24.037222843850298	37.14122449280057	20.252918789199377
39	18.866769971437826	23.573179529460266	37.093559524160064	20.466490974941845
40	18.641051500250285	23.58827036895262	38.16970201113041	19.600976119666676
41	17.56904979240894	23.701819734401226	38.92369188186449	19.805438591325345
42	17.93546273666853	24.278032154530198	38.5991468154648	19.187358293336473
43	18.030608639321574	24.049829215865255	38.08707046317835	19.832491681634817
44	18.763342510526783	24.354774594387678	35.608400076558404	21.273482818527135
45	19.662167633461912	23.641364359117812	35.217050440210826	21.479417567209445
46	19.509234857630812	23.92220120137805	35.91684211301198	20.651721827979152
47	18.01524174788728	24.58417375813433	37.15033420688437	20.250250287094023
48	17.944112364182445	23.854384440976414	37.65449707016872	20.547006124672418
49	19.29704293159801	22.76600365124702	37.092547333706314	20.84440608344866
50	18.583448661700185	22.41781013515503	36.67101601837402	22.327725184770767
51	18.094652689850122	22.426551779982923	35.69333205735991	23.785463472807045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	1040.5
2	1736.0
3	35598.5
4	69461.0
5	49188.5
6	28916.0
7	29145.5
8	29375.0
9	30304.5
10	31234.0
11	31698.5
12	32163.0
13	31910.0
14	31657.0
15	30873.0
16	30089.0
17	29030.0
18	27971.0
19	26594.0
20	25217.0
21	24152.0
22	23087.0
23	22180.5
24	21274.0
25	20334.0
26	18974.5
27	18555.0
28	18292.5
29	18030.0
30	18585.5
31	19141.0
32	20375.5
33	21610.0
34	23138.0
35	24666.0
36	26692.5
37	28719.0
38	31265.5
39	33812.0
40	36659.0
41	39506.0
42	44010.0
43	48514.0
44	52927.0
45	57340.0
46	65427.5
47	73515.0
48	78078.5
49	82642.0
50	79251.0
51	75860.0
52	65360.5
53	54861.0
54	46710.0
55	38559.0
56	32379.5
57	26200.0
58	22546.0
59	18892.0
60	16359.0
61	13826.0
62	11312.5
63	8799.0
64	6876.0
65	4953.0
66	4037.5
67	3122.0
68	2464.0
69	1806.0
70	1329.5
71	853.0
72	616.0
73	379.0
74	334.0
75	226.0
76	163.0
77	126.0
78	89.0
79	78.5
80	68.0
81	52.5
82	37.0
83	28.0
84	19.0
85	12.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1086752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.48650871199999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.33065870352914	40.55614500476422
2	7.726852862556667	7.802036487236184
3	3.3382293028326684	5.056066283403454
4	2.0216311930036537	4.0826040335211955
5	1.3809406798581874	3.4859436832207784
6	1.0509814183702264	3.1836229520819126
7	0.7653980595352012	2.7049593060610277
8	0.5850455390591721	2.36295253637021
9	0.4712981014751381	2.1414776135466256
>10	2.2208682732261247	17.692815547932735
>50	0.06763469791732198	2.400900345163199
>100	0.03663376124801874	3.1662517550449825
>500	0.002916119915031481	1.0541289486275904
>1k	7.290299787578703E-4	0.6856991031051679
>5k	0.0	0.0
>10k+	1.8225749468946757E-4	3.6243963999207542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39389	3.624469980271488	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC	3335	0.30687774211595653	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	1840	0.16931185771914842	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1241	0.1141934866464474	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.600865698889903E-4	0.0	0.0	0.0949618680250876	0.0
2	4.600865698889903E-4	0.0	0.0	0.34902167191778805	0.0
3	4.600865698889903E-4	0.0	0.0	0.5916713288772415	0.0
4	4.600865698889903E-4	0.0	0.0	0.9066465946232443	0.0
5	4.600865698889903E-4	0.0	0.0	1.5695393245193014	0.0
6	4.600865698889903E-4	0.0	0.0	2.505815494243397	0.0
7	4.600865698889903E-4	0.0	0.0	3.338756220370425	0.0
8	4.600865698889903E-4	0.0	0.0	4.503695415329348	0.0
9	4.600865698889903E-4	0.0	0.0	5.185819763846766	0.0
10	4.600865698889903E-4	0.0	0.0	6.266287064574071	0.0
11	4.600865698889903E-4	0.0	0.0	8.199110744677718	0.0
12	4.600865698889903E-4	0.0	0.0	9.687674832896558	0.0
13	5.521038838667883E-4	0.0	0.0	10.285051235240422	0.0
14	5.521038838667883E-4	0.0	0.0	10.525768528606342	0.0
15	6.441211978445864E-4	0.0	0.0	10.787189717617267	0.0
16	6.441211978445864E-4	0.0	0.0	11.3950560937546	0.0
17	6.441211978445864E-4	0.0	0.0	12.207753010806513	0.0
18	7.361385118223845E-4	0.0	0.0	13.031584022849739	0.0
19	7.361385118223845E-4	0.0	0.0	13.525440946968581	0.0
20	7.361385118223845E-4	0.0	0.0	13.95092900680192	0.0
21	7.361385118223845E-4	0.0	0.0	14.451779246783074	0.0
22	7.361385118223845E-4	0.0	0.0	14.90036365242484	0.0
23	7.361385118223845E-4	0.0	0.0	15.295485998645505	0.0
24	7.361385118223845E-4	0.0	0.0	15.567121109507966	0.0
25	7.361385118223845E-4	0.0	0.0	15.784097935867614	0.0
26	7.361385118223845E-4	0.0	0.0	15.972365360266188	0.0
27	7.361385118223845E-4	0.0	0.0	16.147474308765936	0.0
28	8.281558258001826E-4	0.0	0.0	16.32746017490651	0.0
29	9.201731397779806E-4	0.0	0.0	16.511954889431994	0.0
30	9.201731397779806E-4	0.0	0.0	16.703442919819793	0.0
31	9.201731397779806E-4	0.0	0.0	16.886189305379702	0.0
32	9.201731397779806E-4	0.0	0.0	17.062034392391272	0.0
33	9.201731397779806E-4	0.0	0.0	17.242756397043667	0.0
34	9.201731397779806E-4	0.0	0.0	17.407467389063925	0.0
35	0.0010121904537557787	0.0	0.0	17.58110406054003	0.0
36	0.0010121904537557787	0.0	0.0	17.756120991725805	0.0
37	0.0010121904537557787	0.0	0.0	17.941167810135155	0.0
38	0.0010121904537557787	0.0	0.0	18.127042784370307	2.7605194193339416E-4
39	0.0010121904537557787	0.0	0.0	18.32929684049351	2.7605194193339416E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	20	7.033115E-4	45.000004	1
TATAGCG	20	7.033115E-4	45.000004	1
GACGATT	20	7.033115E-4	45.000004	9
TATCCGG	45	3.8562575E-10	45.000004	2
TCGTAAC	25	3.8906648E-5	45.0	34
ACACGCG	30	2.1653923E-6	44.999996	36
CGTTTTT	39790	0.0	44.632442	1
CTTTGCG	220	0.0	40.909092	1
GTTTTTT	44580	0.0	40.341522	2
TCAAGCG	75	0.0	39.0	17
CGGACAG	35	6.247974E-6	38.57143	6
CTGCGCG	70	0.0	38.57143	1
CTACGGG	70	0.0	38.57143	3
TACACGC	35	6.247974E-6	38.57143	35
TTGTCCG	95	0.0	37.894737	1
CGCGGGC	180	0.0	37.500004	4
CTATCCG	30	1.1398811E-4	37.499996	1
CGTAACC	30	1.1398811E-4	37.499996	35
GCTCGTA	30	1.1398811E-4	37.499996	32
TAGCTCG	30	1.1398811E-4	37.499996	30
>>END_MODULE