FastQCFastQC Report
Sat 14 Jan 2017
SRR2933711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069410
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383543.5864635640212827No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC32180.3009135878662066No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC17190.16074283950963617No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC12200.11408159639427348TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGCG207.033067E-445.04
CGTTTTT389050.044.5778161
CTTCGCG556.184564E-1140.9090921
GTTTTTT436100.040.2017862
GCGATCA403.457917E-739.3759
TTGTACG356.247881E-638.5714261
ATGGGCG950.037.8947375
GGCACCG2200.037.840918
GCACCGA2200.037.840919
CTCGTAA301.139869E-437.49999633
TCGTAAC301.139869E-437.49999634
GCGATTC2100.036.428579
TTCTACG250.00210704336.01
GCGACTT250.00210704336.09
CTCGTAC250.00210704336.029
TTCGTAG250.00210704336.01
GTTCGCG250.00210704336.01
TCGCTTG1000.036.01
TCGTACG250.00210704336.030
GCGGACC250.00210704336.05
Produced by FastQC (version 0.11.5)