##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933711.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069410
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60469137187795	34.0	31.0	34.0	31.0	34.0
2	32.83791155870994	34.0	31.0	34.0	31.0	34.0
3	32.83249081269111	34.0	31.0	34.0	31.0	34.0
4	36.281551509710965	37.0	37.0	37.0	35.0	37.0
5	36.34280491111922	37.0	37.0	37.0	35.0	37.0
6	36.34920283146782	37.0	37.0	37.0	35.0	37.0
7	36.35772061230024	37.0	37.0	37.0	35.0	37.0
8	36.31668957649545	37.0	37.0	37.0	35.0	37.0
9	38.182455746626644	39.0	39.0	39.0	37.0	39.0
10	37.60582751236663	39.0	38.0	39.0	35.0	39.0
11	37.62194761597517	39.0	37.0	39.0	35.0	39.0
12	37.688240244620864	39.0	37.0	39.0	35.0	39.0
13	37.75204365023705	39.0	38.0	39.0	35.0	39.0
14	39.20851778083242	41.0	39.0	41.0	36.0	41.0
15	39.23828279144575	41.0	39.0	41.0	36.0	41.0
16	39.29887695084205	41.0	39.0	41.0	36.0	41.0
17	39.28904816674615	41.0	39.0	41.0	36.0	41.0
18	38.674060463246086	39.0	39.0	40.0	36.0	41.0
19	38.01877670865243	38.0	37.0	40.0	35.0	41.0
20	37.23290599489438	38.0	35.0	40.0	35.0	41.0
21	37.172965466939715	38.0	35.0	40.0	35.0	41.0
22	37.15172104244397	38.0	35.0	40.0	35.0	41.0
23	37.03030830083878	37.0	35.0	40.0	34.0	41.0
24	36.92878783628356	37.0	35.0	40.0	34.0	41.0
25	36.89040685985731	37.0	35.0	40.0	34.0	41.0
26	36.85957023031391	37.0	35.0	40.0	34.0	41.0
27	36.79166082232259	37.0	35.0	40.0	34.0	41.0
28	36.664046530329806	37.0	35.0	40.0	33.0	41.0
29	36.389441841763215	37.0	35.0	40.0	33.0	41.0
30	35.93877091106311	37.0	35.0	40.0	32.0	41.0
31	35.231597796916056	37.0	35.0	40.0	27.0	41.0
32	34.13270401436306	37.0	34.0	40.0	18.0	41.0
33	32.57475430377498	37.0	32.0	40.0	10.0	41.0
34	31.320136336858642	37.0	24.0	40.0	7.0	41.0
35	30.560883103767498	37.0	21.0	40.0	7.0	41.0
36	30.13178201064138	37.0	18.0	40.0	7.0	41.0
37	29.90602107704248	37.0	18.0	40.0	7.0	41.0
38	29.76210901338121	37.0	18.0	40.0	7.0	41.0
39	29.684254869507484	37.0	18.0	40.0	7.0	41.0
40	29.561854667526955	37.0	17.0	40.0	7.0	41.0
41	29.43248239683564	37.0	16.0	40.0	7.0	41.0
42	29.265514629562094	36.0	15.0	40.0	7.0	41.0
43	29.16861072928063	36.0	15.0	40.0	7.0	41.0
44	29.019703387849376	36.0	15.0	40.0	7.0	41.0
45	28.961215997606157	36.0	15.0	40.0	7.0	41.0
46	28.794323037936806	35.0	15.0	40.0	7.0	41.0
47	28.76011165034926	35.0	15.0	40.0	7.0	41.0
48	28.63452557952516	35.0	15.0	40.0	7.0	41.0
49	28.60704407102982	35.0	14.0	40.0	7.0	41.0
50	28.454209330378433	35.0	12.0	40.0	7.0	41.0
51	27.79507298416884	35.0	12.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	5.0
12	10.0
13	14.0
14	14.0
15	23.0
16	54.0
17	115.0
18	325.0
19	696.0
20	1380.0
21	2474.0
22	4581.0
23	9410.0
24	21308.0
25	48777.0
26	79398.0
27	75650.0
28	53735.0
29	35583.0
30	25671.0
31	22100.0
32	21221.0
33	23910.0
34	35611.0
35	47010.0
36	60231.0
37	84687.0
38	170869.0
39	244435.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.878951945465257	3.3122936946540618	36.6279537314968	45.18080062838388
2	49.97933439934169	2.924509776418773	35.04212603211116	12.05402979212837
3	13.024845475542591	3.040648581928353	70.489241731422	13.445264211107059
4	11.119308777737256	3.1435090376936814	70.61398341141377	15.123198773155291
5	10.49784460590419	3.9908921741895065	71.07760353839969	14.433659681506624
6	12.713645842099849	4.67949617078576	72.55813953488372	10.04871845223067
7	54.23887938208919	1.1358599601649508	41.97435969366286	2.650900964082999
8	56.197155440850565	1.2220757239973443	40.1658858622979	2.414882972854191
9	51.889266043893365	3.4882785835180146	41.59742287803555	3.0250324945530713
10	22.34643401501763	14.618060425842286	50.3435539222562	12.69195163688389
11	16.955236999841034	17.63392898888172	51.33765347247547	14.073180538801768
12	12.803134438615684	15.75953095632171	56.88248660476337	14.55484800029923
13	14.321261256206693	16.098222384305362	58.03686144696608	11.543654912521859
14	14.306486754378584	16.716881270981197	56.99684872967337	11.97978324496685
15	12.906368932401978	17.395386241011398	57.28495151532154	12.41329331126509
16	12.797056320775008	16.511440887966263	56.853311639128115	13.838191152130614
17	13.33090208619706	17.09045174441982	55.35931027388934	14.219335895493776
18	13.869890874407384	16.1196360610056	56.45271691867478	13.557756145912231
19	14.25533705501164	18.570894231398622	54.79413882421148	12.379629889378254
20	14.882224778148698	18.07594842015691	54.31565068589222	12.72617611580217
21	14.664441140441925	18.970647366304785	54.247014709045175	12.117896784208115
22	14.199605389887882	17.425589811204308	53.78302054403831	14.591784254869507
23	12.911605464695485	18.93380462123975	53.18932869526187	14.965261218802892
24	12.63939929493833	18.504595992182605	53.97303185868844	14.88297285419063
25	12.8136075032027	19.434454512301176	53.18558831505222	14.566349669443898
26	12.862232445928129	19.606512001945	53.90533097689381	13.625924575233073
27	13.261331014297603	19.184223076275703	53.759830186738476	13.794615722688212
28	13.071693737668433	18.11007938956995	53.7319643541766	15.086262518585015
29	13.686051187103168	17.524429358244266	53.09666077556784	15.692858679084729
30	14.710634836031083	17.829457364341085	51.87215380443422	15.587753995193612
31	13.832580581816142	19.416781215810587	50.16710148586604	16.583536716507233
32	14.054198109237802	18.99524036618322	48.764084869226956	18.186476655352017
33	15.644981812401232	19.48335998354233	44.8046118887985	20.067046315257947
34	15.760092013353159	20.328685910922843	42.0069010014868	21.904321074237195
35	16.42101719639801	21.27369297089049	40.381051233857924	21.924238598853574
36	16.83713449472139	22.361021497835253	38.427170121842884	22.374673885600473
37	17.009659531891415	24.068505063539707	37.96439158040415	20.957443824164727
38	18.6391561702247	24.26450098652528	37.09737144780767	19.998971395442346
39	18.851890294648452	23.850534406822455	36.983196341908155	20.314378956620942
40	18.65991528038825	23.81397219027314	38.12560196743999	19.400510561898617
41	17.655997232118644	23.952085729514405	38.762588717143096	19.629328321223852
42	18.00768648133083	24.467697141414423	38.46139460076117	19.06322177649358
43	18.00993070945662	24.25056807024434	38.05827512366632	19.681226096632724
44	18.701433500715346	24.664441140441927	35.5262247407449	21.10790061809783
45	19.652238150007946	23.947316744747106	35.20034411497929	21.20010099026566
46	19.48822247781487	24.16023788818133	35.92251802395713	20.42902161004666
47	18.1047493477712	24.845756071104628	36.99039657381173	20.059098007312443
48	18.012829504119093	24.091975949355252	37.44719050691503	20.448004039610627
49	19.397892295751863	23.03887190132877	36.930456980952115	20.632778821967253
50	18.62952469118486	22.698684321261258	36.537530039928555	22.134260947625325
51	18.17114109649246	22.709344404858754	35.573914588417914	23.545599910230873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	1097.5
2	1860.0
3	35117.0
4	68374.0
5	48483.5
6	28593.0
7	28755.0
8	28917.0
9	29840.5
10	30764.0
11	31059.0
12	31354.0
13	30942.5
14	30531.0
15	29822.5
16	29114.0
17	28107.0
18	27100.0
19	25861.0
20	24622.0
21	23528.5
22	22435.0
23	21713.0
24	20991.0
25	20085.5
26	18703.5
27	18227.0
28	17991.5
29	17756.0
30	18359.5
31	18963.0
32	20140.5
33	21318.0
34	22994.5
35	24671.0
36	26811.5
37	28952.0
38	31476.5
39	34001.0
40	36817.5
41	39634.0
42	43786.5
43	47939.0
44	52159.0
45	56379.0
46	63905.0
47	71431.0
48	76009.5
49	80588.0
50	77606.0
51	74624.0
52	64476.0
53	54328.0
54	46063.5
55	37799.0
56	31741.5
57	25684.0
58	22279.0
59	18874.0
60	16274.0
61	13674.0
62	11232.0
63	8790.0
64	6925.0
65	5060.0
66	4007.0
67	2954.0
68	2329.5
69	1705.0
70	1261.5
71	818.0
72	611.0
73	404.0
74	352.0
75	226.5
76	153.0
77	112.5
78	72.0
79	81.0
80	90.0
81	58.0
82	26.0
83	17.5
84	9.0
85	10.5
86	12.0
87	7.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1069410.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37263676743401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25336737325283	40.425737240563045
2	7.592253082142282	7.64883613506369
3	3.4376327322495834	5.194878748843499
4	2.0567973439821903	4.144252220505515
5	1.462112667128618	3.682523514716701
6	0.9985567586736269	3.0179962137799725
7	0.7703018938172443	2.7161496248965795
8	0.6094349585508746	2.455907664036752
9	0.4732334320727335	2.145421420200536
>10	2.239974490269232	17.905233802683476
>50	0.0660457415351909	2.292170704957208
>100	0.03674554052135471	3.1703258689055955
>500	0.0026113579609701354	0.9207869022638702
>1k	7.46102274562896E-4	0.6761206682337562
>5k	0.0	0.0
>10k+	1.86525568640724E-4	3.6036592703498447
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38354	3.5864635640212827	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC	3218	0.3009135878662066	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	1719	0.16074283950963617	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1220	0.11408159639427348	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.805285157236233E-4	0.0	0.0	0.08658980185335839	0.0
2	2.805285157236233E-4	0.0	0.0	0.33036908201718707	0.0
3	2.805285157236233E-4	0.0	0.0	0.5642363546254476	0.0
4	2.805285157236233E-4	0.0	0.0	0.8769321401520465	0.0
5	2.805285157236233E-4	0.0	0.0	1.514947494412807	0.0
6	2.805285157236233E-4	0.0	0.0	2.4073087029296527	0.0
7	2.805285157236233E-4	0.0	0.0	3.180632311274441	0.0
8	2.805285157236233E-4	0.0	0.0	4.309572568051543	0.0
9	2.805285157236233E-4	0.0	0.0	4.9693756370335045	0.0
10	2.805285157236233E-4	0.0	0.0	6.005741483621811	0.0
11	2.805285157236233E-4	0.0	0.0	7.888274843137805	0.0
12	2.805285157236233E-4	0.0	0.0	9.336363041303148	0.0
13	3.740380209648311E-4	0.0	0.0	9.913877745672847	0.0
14	3.740380209648311E-4	0.0	0.0	10.145781318671043	0.0
15	3.740380209648311E-4	0.0	0.0	10.38105123385792	0.0
16	3.740380209648311E-4	0.0	0.0	10.947718835619641	0.0
17	3.740380209648311E-4	0.0	0.0	11.704397752031493	0.0
18	3.740380209648311E-4	0.0	0.0	12.498106432518865	0.0
19	3.740380209648311E-4	0.0	0.0	12.956396517706025	0.0
20	3.740380209648311E-4	0.0	0.0	13.368492907304027	0.0
21	3.740380209648311E-4	0.0	0.0	13.8463264790866	0.0
22	3.740380209648311E-4	0.0	0.0	14.27759231725905	0.0
23	3.740380209648311E-4	0.0	0.0	14.654996680412564	0.0
24	3.740380209648311E-4	0.0	0.0	14.919815599255664	0.0
25	3.740380209648311E-4	0.0	0.0	15.128154776933075	0.0
26	3.740380209648311E-4	0.0	0.0	15.319475224656586	0.0
27	3.740380209648311E-4	0.0	0.0	15.495553623025781	0.0
28	4.675475262060388E-4	0.0	0.0	15.66817216970105	0.0
29	4.675475262060388E-4	0.0	0.0	15.850048157395198	0.0
30	4.675475262060388E-4	0.0	0.0	16.03996596254009	0.0
31	5.610570314472466E-4	0.0	0.0	16.219691231613695	0.0
32	5.610570314472466E-4	0.0	0.0	16.40353091891791	0.0
33	5.610570314472466E-4	0.0	0.0	16.57886124124517	0.0
34	5.610570314472466E-4	0.0	0.0	16.742315856406805	0.0
35	5.610570314472466E-4	0.0	0.0	16.922134634985646	0.0
36	5.610570314472466E-4	0.0	0.0	17.0949402006714	0.0
37	5.610570314472466E-4	0.0	0.0	17.283268344227192	0.0
38	5.610570314472466E-4	0.0	0.0	17.468323655099542	0.0
39	5.610570314472466E-4	0.0	0.0	17.668433996315727	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGCG	20	7.033067E-4	45.0	4
CGTTTTT	38905	0.0	44.577816	1
CTTCGCG	55	6.184564E-11	40.909092	1
GTTTTTT	43610	0.0	40.201786	2
GCGATCA	40	3.457917E-7	39.375	9
TTGTACG	35	6.247881E-6	38.571426	1
ATGGGCG	95	0.0	37.894737	5
GGCACCG	220	0.0	37.84091	8
GCACCGA	220	0.0	37.84091	9
CTCGTAA	30	1.139869E-4	37.499996	33
TCGTAAC	30	1.139869E-4	37.499996	34
GCGATTC	210	0.0	36.42857	9
TTCTACG	25	0.002107043	36.0	1
GCGACTT	25	0.002107043	36.0	9
CTCGTAC	25	0.002107043	36.0	29
TTCGTAG	25	0.002107043	36.0	1
GTTCGCG	25	0.002107043	36.0	1
TCGCTTG	100	0.0	36.0	1
TCGTACG	25	0.002107043	36.0	30
GCGGACC	25	0.002107043	36.0	5
>>END_MODULE