FastQCFastQC Report
Sat 14 Jan 2017
SRR2933710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841005
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424055.04218167549539No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC20200.24018882170736203No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC11070.13162823050992561No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA9960.1184297358517488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCG650.045.0000041
TACGAAT253.8899096E-545.012
CTTACGG351.2114106E-745.02
CGTTTTT271050.044.4936371
GTACGGC650.041.5384649
GCGATCG1050.040.7142879
GTTTTTT304800.040.083662
CGGGTGA403.456753E-739.3756
CGCATGG403.456753E-739.3752
TGGGCGA13100.038.645046
CTTCGCG356.2462914E-638.5714261
CGGGTCC356.2462914E-638.5714266
CCGCGAG356.2462914E-638.57142616
TCCGCCG301.1396617E-437.4999963
ATCGTCG301.1396617E-437.49999611
GCGGGAC3250.037.3846175
GGCGATT8600.037.1511658
GGCCGAT2700.036.6666648
GCGATTC1350.036.6666649
GCGATTT4550.036.5934079
Produced by FastQC (version 0.11.5)