##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841005
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59279433534878	34.0	31.0	34.0	31.0	34.0
2	32.79165403297245	34.0	31.0	34.0	31.0	34.0
3	32.80923894626073	34.0	31.0	34.0	31.0	34.0
4	36.283028043828516	37.0	37.0	37.0	35.0	37.0
5	36.2987187947753	37.0	37.0	37.0	35.0	37.0
6	36.28689484604729	37.0	37.0	37.0	35.0	37.0
7	36.366277251621575	37.0	37.0	37.0	35.0	37.0
8	36.29849406365004	37.0	37.0	37.0	35.0	37.0
9	38.149227412441064	39.0	39.0	39.0	37.0	39.0
10	37.661242204267516	39.0	38.0	39.0	35.0	39.0
11	37.513354855203	39.0	37.0	39.0	35.0	39.0
12	37.60309035023573	39.0	37.0	39.0	35.0	39.0
13	37.638594300866224	39.0	37.0	39.0	35.0	39.0
14	39.04713883984043	40.0	39.0	41.0	35.0	41.0
15	39.05792712290652	40.0	39.0	41.0	35.0	41.0
16	39.07331704329938	41.0	39.0	41.0	35.0	41.0
17	38.99448398047574	40.0	38.0	41.0	35.0	41.0
18	38.419286448950956	39.0	38.0	40.0	35.0	41.0
19	37.84224112817403	38.0	37.0	40.0	35.0	41.0
20	37.190567238006906	38.0	35.0	40.0	34.0	41.0
21	37.09583296175409	38.0	35.0	40.0	34.0	41.0
22	37.09500538046742	38.0	35.0	40.0	34.0	41.0
23	37.0021165153596	38.0	35.0	40.0	34.0	41.0
24	36.88460591791963	38.0	35.0	40.0	33.0	41.0
25	36.821533760203565	38.0	35.0	40.0	33.0	41.0
26	36.786946569877706	38.0	35.0	40.0	33.0	41.0
27	36.71062835536055	37.0	35.0	40.0	33.0	41.0
28	36.55609657493118	37.0	35.0	40.0	33.0	41.0
29	36.37799656363517	37.0	35.0	40.0	33.0	41.0
30	36.10468903276437	37.0	35.0	40.0	32.0	41.0
31	35.559914625953475	37.0	35.0	40.0	30.0	41.0
32	34.648789246199485	37.0	35.0	40.0	21.0	41.0
33	33.487769989476874	37.0	33.0	40.0	15.0	41.0
34	32.445281538159705	37.0	31.0	40.0	10.0	41.0
35	31.779717124155027	37.0	30.0	40.0	7.0	41.0
36	31.415178268856902	37.0	27.0	40.0	7.0	41.0
37	31.181028650245835	37.0	24.0	40.0	7.0	41.0
38	31.01609859632226	37.0	23.0	40.0	7.0	41.0
39	30.895250325503415	37.0	23.0	40.0	7.0	41.0
40	30.74628212674122	37.0	23.0	40.0	7.0	41.0
41	30.560047799953626	36.0	22.0	40.0	7.0	41.0
42	30.3858098346621	36.0	21.0	40.0	7.0	41.0
43	30.219557553165558	36.0	21.0	40.0	7.0	41.0
44	30.054868877117258	36.0	20.0	40.0	7.0	41.0
45	30.01139945660252	35.0	20.0	40.0	7.0	41.0
46	29.86955606684859	35.0	20.0	40.0	7.0	41.0
47	29.76350437868978	35.0	20.0	40.0	7.0	41.0
48	29.670686856796333	35.0	20.0	40.0	7.0	41.0
49	29.630157965767147	35.0	20.0	40.0	7.0	41.0
50	29.523194273517994	35.0	20.0	40.0	7.0	41.0
51	28.78059821285248	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	6.0
13	8.0
14	9.0
15	29.0
16	66.0
17	125.0
18	302.0
19	659.0
20	1207.0
21	2077.0
22	3526.0
23	6271.0
24	12529.0
25	27376.0
26	45422.0
27	49497.0
28	40423.0
29	30914.0
30	24218.0
31	21204.0
32	21196.0
33	23618.0
34	33622.0
35	45266.0
36	58023.0
37	72171.0
38	136983.0
39	184186.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.722486786642172	3.3314903002954797	36.63842664431246	42.30759626874988
2	46.96666488308631	3.4085409718134847	37.111907777004895	12.512886368095314
3	14.945808883419243	3.562404504134934	68.21445770239178	13.277328910054042
4	11.987086878199298	3.6243541952782685	68.74596464943728	15.642594277085154
5	11.809561179778955	4.223161574544741	68.36071129184725	15.60656595382905
6	14.816439854697652	4.886058941385604	68.89079137460539	11.40670982931136
7	58.88538118084911	1.2903609372120262	37.462678580983464	2.3615793009554045
8	60.954215492178996	1.4074827141336854	35.74746880220688	1.890832991480431
9	56.82201651595412	3.8304171794460196	36.80572648200665	2.541839822593207
10	23.17441632332745	25.270004340045542	41.78358035921308	9.771998977413928
11	16.546869519206187	19.100124256098358	51.638337465294505	12.714668759400954
12	14.199915577196332	19.011064143494984	52.88862729710287	13.900392982205814
13	14.850327881522702	18.713681844935522	54.02215206806142	12.413838205480348
14	15.095391822878579	19.405116497523796	53.037496804418524	12.461994875179101
15	13.817278137466484	18.708568914572446	53.79028662136373	13.683866326597343
16	15.551037151978884	19.53662582267644	52.17055784448368	12.741779180860993
17	15.115249017544485	18.683598789543463	52.494931659145905	13.706220533766148
18	16.385514949376045	17.010362601887028	52.68957972901469	13.914542719722236
19	15.696101687861546	19.135201336496216	51.25688907913746	13.911807896504778
20	17.473498968496028	19.68252269606007	50.54345693545223	12.300521399991677
21	16.98848401614735	20.250176871718956	50.64571554271377	12.11562356941992
22	16.67100671220742	18.18800126039679	50.87829442155516	14.262697605840632
23	14.687189731333344	20.863609609930975	50.332518831635966	14.11668182709972
24	13.81002491067235	20.888579734959958	51.30183530418964	13.999560050178061
25	14.200629009340016	22.434825001040423	49.936445086533375	13.428100903086188
26	14.615965422322102	21.87442405217567	49.77080992384112	13.738800601661108
27	14.065195807397101	21.360752908722304	50.57104297834138	14.003008305539206
28	13.191003620668129	21.261348030035492	50.920505823389874	14.627142525906505
29	14.537131170444884	19.729490312186016	50.96295503593914	14.770423481429956
30	15.643783330657962	19.423903543974173	50.806356680400235	14.125956444967628
31	15.599669443106759	21.376329510526094	48.4026848829674	14.621316163399742
32	15.379337816065302	21.415568278428783	47.71481739109756	15.490276514408357
33	15.96387655245807	22.565501988692098	44.814240105587956	16.65638135326187
34	16.17219873841416	24.179166592350818	43.322453493142135	16.326181176092888
35	16.088727177602987	25.312929174023935	41.477042348142994	17.121301300230083
36	17.374450805881057	26.42576441281562	39.48573432976023	16.714050451543095
37	18.373017996325824	25.941462892610627	38.30464741588932	17.38087169517423
38	17.829025986765835	26.96143304736595	37.75102407238958	17.458516893478635
39	18.751018127121718	25.755257103108782	37.3586363933627	18.1350883764068
40	19.585852640590723	26.06857271954388	38.04543373701702	16.30014090284838
41	18.442458724977854	26.51066283791416	36.79252798734847	18.254350449759514
42	19.91129660346847	26.727427304237196	36.016076004304374	17.345200087989966
43	19.402500579663617	26.317560537690028	36.23652653670311	18.043412345943246
44	19.020576572077456	27.090802076087538	36.012627748943224	17.87599360289178
45	19.58014518344124	25.773568528130035	35.839501548742284	18.806784739686446
46	18.943288089844888	26.293541655519288	36.217858395610016	18.54531185902581
47	17.760060879542927	27.379147567493657	37.025939203690825	17.834852349272595
48	18.642576441281562	26.558819507612913	36.58670281389528	18.211901237210242
49	19.599883472749866	25.900678355063288	36.27517077781939	18.224267394367452
50	18.33021206770471	26.16964227323262	36.12820375622023	19.371941902842433
51	18.00583825304249	25.308767486519105	35.46007455365901	21.225319706779388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	479.0
1	1254.5
2	2030.0
3	35843.0
4	69656.0
5	47031.0
6	24406.0
7	23953.0
8	23500.0
9	23513.0
10	23526.0
11	23158.0
12	22790.0
13	22076.0
14	21362.0
15	20522.0
16	19682.0
17	18537.5
18	17393.0
19	16313.0
20	15233.0
21	14509.5
22	13786.0
23	13296.5
24	12807.0
25	12627.0
26	12792.0
27	13137.0
28	13791.5
29	14446.0
30	15619.5
31	16793.0
32	18030.5
33	19268.0
34	21528.5
35	23789.0
36	25797.0
37	27805.0
38	29773.5
39	31742.0
40	33733.5
41	35725.0
42	37727.0
43	39729.0
44	41724.0
45	43719.0
46	46464.5
47	49210.0
48	50728.5
49	52247.0
50	51260.0
51	50273.0
52	45537.0
53	40801.0
54	35462.0
55	30123.0
56	26276.0
57	22429.0
58	19423.0
59	16417.0
60	14311.5
61	12206.0
62	10375.5
63	8545.0
64	6708.0
65	4871.0
66	3796.5
67	2722.0
68	2129.0
69	1536.0
70	1260.5
71	985.0
72	978.5
73	972.0
74	1031.5
75	974.5
76	858.0
77	575.5
78	293.0
79	199.0
80	105.0
81	79.0
82	53.0
83	33.0
84	13.0
85	8.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	841005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.07905602120651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1342607528834	34.77139661633582
2	8.24251825303456	7.431298841687243
3	3.8423068468811445	5.196226968036613
4	2.3048030533750916	4.1559338384377424
5	1.713810739110085	3.862848515904443
6	1.2755041550349695	3.449911395606183
7	0.9491375641901608	2.995035779957182
8	0.7686326265812603	2.771938658670699
9	0.639518348456351	2.594599511098798
>10	3.0141233004030026	21.27489350764218
>50	0.07560595722533021	2.3653414758957663
>100	0.03635377211849458	2.929521921736515
>500	0.0026343313124276746	0.7939836866726567
>1k	5.268662624855349E-4	0.3713411588730777
>5k	0.0	0.0
>10k+	2.6343313124276745E-4	5.035728123445111
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42405	5.04218167549539	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC	2020	0.24018882170736203	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC	1107	0.13162823050992561	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	996	0.1184297358517488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.945267864043614E-4	0.0	0.0	0.07978549473546531	0.0
2	5.945267864043614E-4	0.0	0.0	0.24827438600246135	0.0
3	7.134321436852337E-4	0.0	0.0	0.40154339153750573	0.0
4	7.134321436852337E-4	0.0	0.0	0.5876302756820708	0.0
5	7.134321436852337E-4	0.0	0.0	0.9928597332952837	0.0
6	7.134321436852337E-4	0.0	0.0	1.5504069535852938	0.0
7	7.134321436852337E-4	0.0	0.0	2.0446965238018797	0.0
8	7.134321436852337E-4	0.0	0.0	2.762409260349225	0.0
9	7.134321436852337E-4	0.0	0.0	3.2156764823039103	0.0
10	7.134321436852337E-4	0.0	0.0	3.8659698812730006	0.0
11	7.134321436852337E-4	0.0	0.0	5.015071254035351	0.0
12	7.134321436852337E-4	0.0	0.0	5.919465401513666	0.0
13	7.134321436852337E-4	0.0	0.0	6.270117300134958	0.0
14	7.134321436852337E-4	0.0	0.0	6.415776362804026	0.0
15	7.134321436852337E-4	0.0	0.0	6.576417500490485	0.0
16	7.134321436852337E-4	0.0	0.0	6.910898270521578	0.0
17	7.134321436852337E-4	0.0	0.0	7.367019221051004	0.0
18	7.134321436852337E-4	0.0	0.0	7.845018757320111	0.0
19	7.134321436852337E-4	0.0	0.0	8.140855286234922	0.0
20	7.134321436852337E-4	0.0	0.0	8.408392340116883	0.0
21	7.134321436852337E-4	0.0	0.0	8.729912426204363	0.0
22	7.134321436852337E-4	0.0	0.0	9.031218601554093	0.0
23	7.134321436852337E-4	0.0	0.0	9.286508403636125	0.0
24	7.134321436852337E-4	0.0	0.0	9.470930612778758	0.0
25	7.134321436852337E-4	0.0	0.0	9.620632457595377	0.0
26	7.134321436852337E-4	0.0	0.0	9.7547577006082	0.0
27	7.134321436852337E-4	0.0	0.0	9.891855577553047	0.0
28	7.134321436852337E-4	0.0	0.0	10.024910672350343	0.0
29	7.134321436852337E-4	0.0	0.0	10.179249826100914	0.0
30	7.134321436852337E-4	0.0	0.0	10.349522297727123	0.0
31	7.134321436852337E-4	0.0	0.0	10.499699763972865	0.0
32	7.134321436852337E-4	0.0	0.0	10.664383683806873	0.0
33	7.134321436852337E-4	0.0	0.0	10.809448219689537	0.0
34	7.134321436852337E-4	0.0	0.0	10.935725709121824	0.0
35	7.134321436852337E-4	0.0	0.0	11.09208625394617	0.0
36	7.134321436852337E-4	0.0	0.0	11.217412500520211	0.0
37	7.134321436852337E-4	0.0	0.0	11.366282007835864	0.0
38	7.134321436852337E-4	0.0	0.0	11.523712700875738	0.0
39	7.134321436852337E-4	0.0	0.0	11.720738877890144	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTTCG	65	0.0	45.000004	1
TACGAAT	25	3.8899096E-5	45.0	12
CTTACGG	35	1.2114106E-7	45.0	2
CGTTTTT	27105	0.0	44.493637	1
GTACGGC	65	0.0	41.538464	9
GCGATCG	105	0.0	40.714287	9
GTTTTTT	30480	0.0	40.08366	2
CGGGTGA	40	3.456753E-7	39.375	6
CGCATGG	40	3.456753E-7	39.375	2
TGGGCGA	1310	0.0	38.64504	6
CTTCGCG	35	6.2462914E-6	38.571426	1
CGGGTCC	35	6.2462914E-6	38.571426	6
CCGCGAG	35	6.2462914E-6	38.571426	16
TCCGCCG	30	1.1396617E-4	37.499996	3
ATCGTCG	30	1.1396617E-4	37.499996	11
GCGGGAC	325	0.0	37.384617	5
GGCGATT	860	0.0	37.151165	8
GGCCGAT	270	0.0	36.666664	8
GCGATTC	135	0.0	36.666664	9
GCGATTT	455	0.0	36.593407	9
>>END_MODULE