FastQCFastQC Report
Sat 14 Jan 2017
SRR2933709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846788
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420634.967359008394072No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC19710.2327619191580419No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC10900.1287217107469638No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10370.12246276517853347No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA8820.10415830172368998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA207.032233E-445.018
CGTTTTT271250.044.460831
GCTCTCG900.042.51
GCGATGC2350.040.212769
GTTTTTT305650.040.14152
CGGGACG451.9272193E-840.06
GCGTTTG1600.039.3751
GGACCGA3600.038.758
GCCTTCG356.2463387E-638.571431
CGTTAGG356.2463387E-638.571432
TGGGCGA13200.038.3522726
CTTCGCG659.094947E-1238.0769271
GGGACCG4300.037.674427
AGGCACG2750.037.63636410
ATCGTCG301.1396682E-437.49999611
GCGATTC1200.037.4999969
GACCGAT3550.037.3943679
GCTTTCG1450.037.241381
CTCTGCG1150.037.1739161
TTGGGCG10550.036.8957375
Produced by FastQC (version 0.11.5)