##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933709.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846788
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5132477078088	34.0	31.0	34.0	31.0	34.0
2	32.73658932341979	34.0	31.0	34.0	31.0	34.0
3	32.75995290438693	34.0	31.0	34.0	31.0	34.0
4	36.2078879247226	37.0	37.0	37.0	35.0	37.0
5	36.27530975875898	37.0	37.0	37.0	35.0	37.0
6	36.2836932030213	37.0	37.0	37.0	35.0	37.0
7	36.304002890924295	37.0	37.0	37.0	35.0	37.0
8	36.261860111385616	37.0	37.0	37.0	35.0	37.0
9	38.12234349093279	39.0	39.0	39.0	37.0	39.0
10	37.67089755641317	39.0	38.0	39.0	35.0	39.0
11	37.516450398446835	39.0	37.0	39.0	35.0	39.0
12	37.58545468287222	39.0	37.0	39.0	35.0	39.0
13	37.6356183602035	39.0	37.0	39.0	35.0	39.0
14	39.066525505793656	40.0	39.0	41.0	35.0	41.0
15	39.07371384573234	41.0	39.0	41.0	35.0	41.0
16	39.12517300670298	41.0	39.0	41.0	35.0	41.0
17	39.07098707114414	41.0	39.0	41.0	35.0	41.0
18	38.52885373907046	39.0	38.0	41.0	35.0	41.0
19	37.981510130044356	38.0	37.0	41.0	35.0	41.0
20	37.29360595568194	38.0	35.0	41.0	34.0	41.0
21	37.2214840078036	38.0	35.0	41.0	34.0	41.0
22	37.20414554764593	38.0	35.0	41.0	34.0	41.0
23	37.06227178467338	38.0	35.0	40.0	34.0	41.0
24	36.961015035640564	38.0	35.0	40.0	34.0	41.0
25	36.91142765367483	38.0	35.0	40.0	33.0	41.0
26	36.89200602748267	38.0	35.0	40.0	33.0	41.0
27	36.82203692069326	38.0	35.0	40.0	33.0	41.0
28	36.72167177617066	38.0	35.0	40.0	33.0	41.0
29	36.49622337586267	38.0	35.0	40.0	33.0	41.0
30	36.134453960141144	37.0	35.0	40.0	32.0	41.0
31	35.59964949904817	37.0	35.0	40.0	30.0	41.0
32	34.753623102830936	38.0	35.0	40.0	21.0	41.0
33	33.5561958837395	38.0	33.0	40.0	15.0	41.0
34	32.52557428777923	37.0	32.0	40.0	10.0	41.0
35	31.906276423378696	37.0	30.0	40.0	7.0	41.0
36	31.540348942120104	37.0	27.0	40.0	7.0	41.0
37	31.34118929413265	37.0	25.0	40.0	7.0	41.0
38	31.158661908293457	37.0	24.0	40.0	7.0	41.0
39	31.074526327723113	37.0	23.0	40.0	7.0	41.0
40	30.91983471659967	37.0	23.0	40.0	7.0	41.0
41	30.74512274618913	37.0	23.0	40.0	7.0	41.0
42	30.524292975337392	36.0	22.0	40.0	7.0	41.0
43	30.391311638804517	36.0	21.0	40.0	7.0	41.0
44	30.21578010080445	36.0	21.0	40.0	7.0	41.0
45	30.15549110284983	36.0	20.0	40.0	7.0	41.0
46	30.002198897480834	35.0	20.0	40.0	7.0	41.0
47	29.965392754739085	35.0	20.0	40.0	7.0	41.0
48	29.848584297368408	35.0	20.0	40.0	7.0	41.0
49	29.77780861325385	35.0	20.0	40.0	7.0	41.0
50	29.62372754455661	35.0	20.0	40.0	7.0	41.0
51	28.975338573527257	35.0	19.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	10.0
13	7.0
14	14.0
15	26.0
16	42.0
17	129.0
18	319.0
19	693.0
20	1204.0
21	2058.0
22	3372.0
23	6058.0
24	12618.0
25	26991.0
26	44932.0
27	49058.0
28	40494.0
29	30840.0
30	24210.0
31	21333.0
32	21035.0
33	23306.0
34	33011.0
35	44602.0
36	57644.0
37	70488.0
38	133695.0
39	198504.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.81059722150054	3.4673377515978023	36.63443506521113	42.08762996169053
2	46.86355971034072	3.458008379901463	37.17447578378532	12.503956125972499
3	15.1829029225733	3.622394271057219	67.81142387468883	13.383278931680657
4	12.152746614264727	3.654988025338101	68.50770204584855	15.684563314548624
5	11.83165089727299	4.244746028521897	68.2878122977652	15.635790776439912
6	14.919200555510942	4.957793450072509	68.65472822005036	11.468277774366193
7	58.987727742953375	1.3375248586423047	37.230570107275966	2.444177291128358
8	61.09250485363514	1.4456983329947992	35.54337094998984	1.9184258633802087
9	57.01202662295639	3.875586333297118	36.54161372149818	2.5707733222483076
10	23.262374998228598	25.338927807196132	41.56530324000812	9.833393954567141
11	16.550895855869474	19.320892596494048	51.422315857097644	12.705895690538835
12	14.30523342324171	19.07053477375683	52.75523507654809	13.868996726453375
13	15.048867012758802	18.763964534216356	53.71580608133323	12.471362371691615
14	15.145113062537494	19.558496341469176	52.84286031450611	12.45353028148722
15	13.921312063940444	18.861155330495944	53.52083402221099	13.696698583352621
16	15.545685578917038	19.619668677402135	52.04372286806142	12.790922875619398
17	15.210536757724483	18.66866323093856	52.39800280589711	13.722797205439852
18	16.468584816978986	17.10498967864448	52.52684260995668	13.899582894419854
19	15.753765995739194	19.224882733340575	51.14160805301917	13.879743217901055
20	17.56779737077049	19.72040227306008	50.36325502959419	12.348545326575247
21	16.98181835359027	20.345352083402222	50.4805216890178	12.192307873989712
22	16.88427327737285	18.20030515311979	50.69261727846875	14.222804291038607
23	14.79874537664681	20.89732022655021	50.16722013065844	14.136714266144537
24	13.96713226923386	20.981756945067715	51.14208042626962	13.909030359428806
25	14.29897447767328	22.516261449146658	49.819435325016414	13.365328748163648
26	14.641326990935157	22.024520895430733	49.632257424526564	13.701894689107547
27	14.128683920886928	21.472670845595356	50.49115008715287	13.907495146364852
28	13.325295115188219	21.354695626296074	50.68836591921473	14.631643339300982
29	14.68195109047365	19.849478263744878	50.69131825203003	14.777252393751446
30	15.73061970646726	19.516691308804564	50.61408522558185	14.138603759146326
31	15.611935927292308	21.5317175019013	48.305242870706714	14.551103700099672
32	15.526318275648688	21.537622167531897	47.5483828301771	15.387676726642324
33	16.0269158278105	22.703203163011285	44.6222667302796	16.647614278898615
34	16.16083364431239	24.264987222303574	43.17172657146771	16.402452561916324
35	16.159652711186272	25.40187154281827	41.42807881075311	17.01039693524235
36	17.44261845940188	26.50722494886559	39.42498004223017	16.625176549502356
37	18.43188613915171	26.028002286286533	38.222435839903255	17.317675734658497
38	17.885232195071257	26.991407530574357	37.708257556791075	17.41510271756331
39	18.886899672645338	25.78402150243036	37.33803502175279	17.991043803171515
40	19.593924335252744	26.093662168098746	38.014355423081106	16.298058073567407
41	18.4565676414876	26.55918600641483	36.80661511499927	18.177631237098304
42	19.93922918132992	26.805882936461074	35.98220569965564	17.272682182553368
43	19.408872114389904	26.36362348072953	36.31770880078603	17.90979560409453
44	19.023179355399463	27.08080416822156	36.093567693448655	17.80244878293032
45	19.691705598095393	25.89998913541524	35.76231595157229	18.645989314917074
46	18.946418702201733	26.442509813554278	36.25618218491523	18.354889299328757
47	17.775169227716972	27.47098447309126	36.9876521632333	17.766194135958468
48	18.718498608860777	26.68495538434649	36.56476001077011	18.031785996022617
49	19.58990916262394	26.076420544457406	36.221226564382114	18.11244372853654
50	18.37827177522591	26.29123228009844	36.062627245544334	19.267868699131306
51	18.04123346103157	25.47721507626466	35.47275114904793	21.008800313655836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	453.0
1	1263.5
2	2074.0
3	35852.5
4	69631.0
5	47196.0
6	24761.0
7	24250.5
8	23740.0
9	23680.0
10	23620.0
11	23272.5
12	22925.0
13	22374.0
14	21823.0
15	20648.0
16	19473.0
17	18383.5
18	17294.0
19	16204.0
20	15114.0
21	14409.0
22	13704.0
23	13281.0
24	12858.0
25	12652.5
26	12891.0
27	13335.0
28	13967.0
29	14599.0
30	15631.5
31	16664.0
32	18029.5
33	19395.0
34	21681.0
35	23967.0
36	25941.5
37	27916.0
38	29946.5
39	31977.0
40	34215.0
41	36453.0
42	38333.0
43	40213.0
44	42352.0
45	44491.0
46	47020.0
47	49549.0
48	51122.0
49	52695.0
50	51670.5
51	50646.0
52	45845.5
53	41045.0
54	35660.5
55	30276.0
56	26487.0
57	22698.0
58	19611.0
59	16524.0
60	14308.5
61	12093.0
62	10370.0
63	8647.0
64	6805.5
65	4964.0
66	3859.0
67	2754.0
68	2129.0
69	1504.0
70	1257.0
71	1010.0
72	993.0
73	976.0
74	1025.5
75	975.5
76	876.0
77	609.0
78	342.0
79	225.0
80	108.0
81	86.5
82	65.0
83	38.0
84	11.0
85	6.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	846788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.20015902833771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19165741955416	34.89075191024813
2	8.147527108644018	7.365390419968043
3	3.893661478227297	5.279823540551589
4	2.3581961715955413	4.263633679045427
5	1.6711165485174013	3.7767366873936683
6	1.271277976091838	3.447718001314463
7	0.9530489497792051	3.0154574864267216
8	0.7812958248010535	2.825175642334712
9	0.6343263405670182	2.5804486322543454
>10	2.9871313077073958	21.283967205407833
>50	0.07349084026665618	2.3151700178105585
>100	0.03412045388979017	2.858738219132596
>500	0.0020997202390924384	0.6206962842418636
>1k	7.873950896596644E-4	0.48616463547843214
>5k	0.0	0.0
>10k+	2.624650298865548E-4	4.990127638391726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42063	4.967359008394072	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC	1971	0.2327619191580419	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC	1090	0.1287217107469638	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1037	0.12246276517853347	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	882	0.10415830172368998	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1809331261189341E-4	0.0	0.0	0.0788863328247448	0.0
2	1.1809331261189341E-4	0.0	0.0	0.24374459723094802	0.0
3	1.1809331261189341E-4	0.0	0.0	0.3943135708111121	0.0
4	1.1809331261189341E-4	0.0	0.0	0.5788934184235015	0.0
5	1.1809331261189341E-4	0.0	0.0	0.9570282054067842	0.0
6	1.1809331261189341E-4	0.0	0.0	1.4980136704818678	0.0
7	1.1809331261189341E-4	0.0	0.0	1.9903446907608515	0.0
8	1.1809331261189341E-4	0.0	0.0	2.6837886224178895	0.0
9	1.1809331261189341E-4	0.0	0.0	3.1305356240286826	0.0
10	2.3618662522378682E-4	0.0	0.0	3.7735537112004423	0.0
11	2.3618662522378682E-4	0.0	0.0	4.913980831093497	0.0
12	2.3618662522378682E-4	0.0	0.0	5.809128140691649	0.0
13	2.3618662522378682E-4	0.0	0.0	6.16033765239942	0.0
14	2.3618662522378682E-4	0.0	0.0	6.296735428466157	0.0
15	2.3618662522378682E-4	0.0	0.0	6.457932800181391	0.0
16	3.5427993783568025E-4	0.0	0.0	6.79213687487305	0.0
17	4.7237325044757365E-4	0.0	0.0	7.258014993126969	0.0
18	4.7237325044757365E-4	0.0	0.0	7.72460167125656	0.0
19	4.7237325044757365E-4	0.0	0.0	8.006018035210703	0.0
20	4.7237325044757365E-4	0.0	0.0	8.277514560905445	0.0
21	4.7237325044757365E-4	0.0	0.0	8.575818268563088	0.0
22	4.7237325044757365E-4	0.0	0.0	8.866209724275734	0.0
23	4.7237325044757365E-4	0.0	0.0	9.118929413265185	0.0
24	4.7237325044757365E-4	0.0	0.0	9.288747596801088	0.0
25	4.7237325044757365E-4	0.0	0.0	9.432349064937151	0.0
26	4.7237325044757365E-4	0.0	0.0	9.551269030737327	0.0
27	4.7237325044757365E-4	0.0	0.0	9.687194433553616	0.0
28	4.7237325044757365E-4	0.0	0.0	9.818041823927596	0.0
29	4.7237325044757365E-4	0.0	0.0	9.963532785065448	0.0
30	4.7237325044757365E-4	0.0	0.0	10.141145127233735	0.0
31	4.7237325044757365E-4	0.0	0.0	10.289588421186885	0.0
32	4.7237325044757365E-4	0.0	0.0	10.450077233026448	0.0
33	4.7237325044757365E-4	0.0	0.0	10.590844461659826	0.0
34	4.7237325044757365E-4	0.0	0.0	10.727360331039174	0.0
35	4.7237325044757365E-4	0.0	0.0	10.89304524863366	0.0
36	5.904665630594671E-4	0.0	0.0	11.02271170588152	0.0
37	5.904665630594671E-4	0.0	0.0	11.156393335758183	0.0
38	7.085598756713605E-4	0.0	0.0	11.314402188032895	0.0
39	7.085598756713605E-4	0.0	0.0	11.503115301586702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGA	20	7.032233E-4	45.0	18
CGTTTTT	27125	0.0	44.46083	1
GCTCTCG	90	0.0	42.5	1
GCGATGC	235	0.0	40.21276	9
GTTTTTT	30565	0.0	40.1415	2
CGGGACG	45	1.9272193E-8	40.0	6
GCGTTTG	160	0.0	39.375	1
GGACCGA	360	0.0	38.75	8
GCCTTCG	35	6.2463387E-6	38.57143	1
CGTTAGG	35	6.2463387E-6	38.57143	2
TGGGCGA	1320	0.0	38.352272	6
CTTCGCG	65	9.094947E-12	38.076927	1
GGGACCG	430	0.0	37.67442	7
AGGCACG	275	0.0	37.636364	10
ATCGTCG	30	1.1396682E-4	37.499996	11
GCGATTC	120	0.0	37.499996	9
GACCGAT	355	0.0	37.394367	9
GCTTTCG	145	0.0	37.24138	1
CTCTGCG	115	0.0	37.173916	1
TTGGGCG	1055	0.0	36.895737	5
>>END_MODULE