SRR2933708.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933708.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101668
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3725	3.6638863752606525	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	1451	1.4271943974505252	No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	884	0.8694967934846757	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	732	0.7199905575008853	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	692	0.6806468111893614	No Hit
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	598	0.5881890073572805	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	525	0.5163866703387496	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	355	0.34917574851477357	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	352	0.3462249675414093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	334	0.32852028170122355	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	289	0.2842585671007593	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	247	0.24294763347365939	No Hit
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	209	0.20557107447771178	No Hit
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	175	0.17212889011291654	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	172	0.16917810913955228	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.15048982964157848	No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	135	0.13278514380139278	No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	135	0.13278514380139278	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC	124	0.12196561356572373	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	115	0.11311327064563088	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	104	0.10229374040996184	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGGA	25	3.865679E-5	45.000004	4
TGTTCGG	30	2.1459618E-6	45.000004	2
CGGGATT	25	3.865679E-5	45.000004	6
TGGAATG	25	3.865679E-5	45.000004	6
TGGGTGA	30	2.1459618E-6	45.000004	6
TGGGTAC	25	3.865679E-5	45.000004	6
GGTGATA	20	7.0029486E-4	45.0	8
TTGTTCG	20	7.0029486E-4	45.0	1
GGGTCTA	20	7.0029486E-4	45.0	7
CCTATGT	20	7.0029486E-4	45.0	10
CGGGGTT	20	7.0029486E-4	45.0	6
GTTCTTG	45	3.765308E-10	45.0	1
TGTTCAT	20	7.0029486E-4	45.0	14
GGCCCAC	20	7.0029486E-4	45.0	8
GGCCTAT	20	7.0029486E-4	45.0	8
TGCTCGG	20	7.0029486E-4	45.0	2
ATGCTGG	20	7.0029486E-4	45.0	2
CCCTGGA	20	7.0029486E-4	45.0	3
TCGTTGA	20	7.0029486E-4	45.0	24
TGGGCGA	80	0.0	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content