##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933708.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101668
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945823661329033	33.0	31.0	34.0	30.0	34.0
2	32.124326238344416	33.0	31.0	34.0	30.0	34.0
3	32.255980249439354	33.0	31.0	34.0	30.0	34.0
4	35.86181492701735	37.0	35.0	37.0	35.0	37.0
5	35.86675256717945	37.0	35.0	37.0	35.0	37.0
6	35.80383208089074	37.0	35.0	37.0	35.0	37.0
7	35.824251485226426	37.0	35.0	37.0	35.0	37.0
8	35.582257937600815	37.0	35.0	37.0	33.0	37.0
9	37.432220561041824	39.0	37.0	39.0	34.0	39.0
10	37.00403273399693	39.0	37.0	39.0	33.0	39.0
11	36.98443954833379	39.0	37.0	39.0	33.0	39.0
12	37.02524884919542	39.0	37.0	39.0	33.0	39.0
13	36.95096785615927	39.0	37.0	39.0	33.0	39.0
14	37.99152142266987	40.0	37.0	41.0	33.0	41.0
15	38.01256049100996	40.0	37.0	41.0	33.0	41.0
16	38.069638430971395	40.0	37.0	41.0	33.0	41.0
17	38.04421253491758	40.0	37.0	41.0	33.0	41.0
18	37.82673014124405	39.0	37.0	40.0	33.0	41.0
19	37.62708030058622	39.0	37.0	40.0	33.0	41.0
20	37.22080694023685	39.0	35.0	40.0	33.0	41.0
21	37.154453712082464	39.0	35.0	40.0	33.0	41.0
22	37.16153558641854	39.0	35.0	40.0	33.0	41.0
23	37.090795530550416	39.0	35.0	40.0	33.0	41.0
24	36.96457095644647	38.0	35.0	40.0	32.0	41.0
25	36.876126214738164	38.0	35.0	40.0	32.0	41.0
26	36.82821536766731	38.0	35.0	40.0	32.0	41.0
27	36.69063028681591	38.0	35.0	40.0	32.0	41.0
28	36.59955935004131	38.0	35.0	40.0	32.0	41.0
29	36.515019475154425	38.0	35.0	40.0	31.0	41.0
30	36.307717275839	38.0	35.0	40.0	31.0	41.0
31	36.09531022543967	38.0	35.0	40.0	30.0	41.0
32	35.753413069992526	38.0	35.0	40.0	29.0	41.0
33	35.38526379981902	38.0	35.0	40.0	27.0	41.0
34	35.10819530235669	38.0	35.0	40.0	24.0	41.0
35	34.789206043199435	38.0	34.0	40.0	22.0	41.0
36	34.52473738049337	38.0	34.0	40.0	21.0	41.0
37	34.41227328166188	38.0	34.0	40.0	21.0	41.0
38	34.361391981744504	38.0	34.0	40.0	21.0	41.0
39	34.16610929692725	38.0	33.0	40.0	20.0	41.0
40	33.97391509619546	38.0	33.0	40.0	18.0	41.0
41	33.84500531140575	37.0	33.0	40.0	18.0	41.0
42	33.74202305543534	37.0	33.0	40.0	18.0	41.0
43	33.63660148719361	37.0	33.0	40.0	18.0	41.0
44	33.504062241806665	37.0	33.0	40.0	18.0	41.0
45	33.42089939804068	37.0	33.0	40.0	18.0	41.0
46	33.04859936263131	36.0	32.0	40.0	17.0	41.0
47	32.794950230160914	36.0	32.0	39.0	17.0	41.0
48	32.713823425266554	36.0	32.0	39.0	16.0	41.0
49	32.72767242396821	36.0	32.0	39.0	17.0	40.0
50	32.56596962662785	35.0	32.0	39.0	15.0	40.0
51	31.403716016839123	35.0	30.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.0
14	4.0
15	8.0
16	10.0
17	26.0
18	33.0
19	87.0
20	151.0
21	228.0
22	355.0
23	536.0
24	758.0
25	1385.0
26	2133.0
27	2571.0
28	2471.0
29	2518.0
30	2583.0
31	2915.0
32	3528.0
33	4431.0
34	6346.0
35	8831.0
36	11439.0
37	14266.0
38	20378.0
39	13671.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.548215761104775	5.098949521973482	42.028956997285285	29.323877719636464
2	33.319235157571704	6.8074517055514026	41.393555494354175	18.47975764252272
3	17.83845457764488	7.014006373686903	55.822874454105516	19.324664594562694
4	15.406027461934926	5.275996380375339	57.687768029271744	21.630208128417987
5	14.807018924341975	6.239918165007672	55.97434787740488	22.978715033245464
6	17.289609316599126	8.789392925994413	59.311681158279896	14.609316599126569
7	60.72117086989023	3.4543809261517877	29.069126962269348	6.755321241688633
8	58.95365306684502	5.09993311563127	27.362591966007006	8.583821851516701
9	53.87732619900066	6.107133021206279	30.29271747255774	9.722823307235315
10	28.238973915096192	21.357752685210684	35.13789983082189	15.265373568871226
11	19.38269662037219	19.393516150607862	39.715544714167685	21.508242514852263
12	17.26108510052327	17.209938230318294	45.00531140575206	20.523665263406382
13	19.400401306212377	19.092536491324704	45.76759649053783	15.73946571192509
14	16.599126568831885	21.86725419994492	43.4404138962112	18.093205335012
15	12.645080064523745	20.832513671951844	45.859070700712124	20.66333556281229
16	12.855569107290396	21.176771452177675	42.10272652161939	23.864932918912537
17	12.651965220128261	21.278081598929848	41.19683676279655	24.873116418145337
18	13.733918243695165	19.814494236141165	44.78892080103867	21.662666719124996
19	13.759491678797655	21.801353424873117	43.969587284101195	20.469567612228037
20	15.112916551914074	24.904591415194556	42.03092418460086	17.951567848290516
21	15.030294684659873	26.2806389424401	41.475193767950586	17.213872604949444
22	12.541802730455995	24.569185977888814	39.053586182476295	23.835425109178896
23	13.743754180273045	24.50918676476374	37.54376991777157	24.203289137191643
24	15.356847779045522	23.791163394578433	37.16508635952315	23.686902466852892
25	13.98965259472007	26.009167092890586	34.63626706534996	25.364913247039382
26	14.800133768737458	26.34063815556517	37.14344729905182	21.715780776645552
27	15.67749931148444	25.88129991737813	37.55852382263839	20.882676948499036
28	13.894244009914624	23.250186882794978	37.194594169256796	25.660974938033597
29	18.815163079828462	22.25479010111343	35.24117716488964	23.68886965416847
30	20.616123067238462	26.550143604674037	33.376283589723414	19.457449738364087
31	19.25974741314868	28.96093165991266	29.905181571389228	21.874139355549435
32	19.819412204430105	29.05240587008695	27.941928630444192	23.186253295038753
33	19.451548176417358	26.648502970452846	28.41110280520911	25.48884604792068
34	19.833182515639137	22.987567376165558	31.264508006452374	25.914742101742927
35	22.756422866585357	26.39375221308573	27.361608372349217	23.488216547979697
36	20.901365227997008	30.297635440846676	27.401935712318526	21.399063618837786
37	18.49549514104733	28.795687925404255	29.535350356060903	23.17346657748751
38	21.04595349569186	28.610772317740096	26.142935830349767	24.20033835621828
39	22.90396191525357	26.472439705708776	29.1222410197899	21.50135735924775
40	20.506944171223985	26.948499036078218	32.83038910965102	19.714167683046778
41	17.44304992721407	29.856001888499822	28.93732541212574	23.763622772160364
42	19.065979462564425	26.556045166620766	28.61470669237125	25.763268678443563
43	20.434158240547664	23.070189243419758	30.93008616280442	25.565566353228153
44	19.984655938938506	22.543966636503125	30.03403234055947	27.4373450839989
45	23.262973600346225	21.151198017075185	30.11763780147146	25.46819058110713
46	22.77117677145218	23.801982924814098	33.74513121139395	19.681709092339773
47	15.768973521658733	26.444899083290714	36.45001377031121	21.336113624739347
48	15.975528189794233	24.047881339261124	36.2936223787229	23.68296809222174
49	20.672187905732383	19.890230947790847	36.99787543769918	22.43970570877759
50	20.18530904512728	18.05779596333163	34.07561868041075	27.681276311130347
51	17.79812723767557	18.10795923987882	32.002203249793446	32.091710272652165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	441.0
1	536.0
2	631.0
3	3151.5
4	5672.0
5	3522.0
6	1372.0
7	1389.0
8	1406.0
9	1434.5
10	1463.0
11	1403.5
12	1344.0
13	1257.5
14	1171.0
15	1105.5
16	1040.0
17	952.5
18	865.0
19	815.0
20	765.0
21	742.5
22	720.0
23	685.5
24	651.0
25	631.0
26	646.5
27	682.0
28	706.0
29	730.0
30	763.5
31	797.0
32	862.0
33	927.0
34	1028.5
35	1130.0
36	1330.5
37	1531.0
38	1690.0
39	1849.0
40	2469.5
41	3090.0
42	4103.0
43	5116.0
44	5559.5
45	6003.0
46	8572.0
47	11141.0
48	12853.0
49	14565.0
50	13642.5
51	12720.0
52	10168.5
53	7617.0
54	5908.0
55	4199.0
56	3499.5
57	2800.0
58	2471.0
59	2142.0
60	2012.5
61	1883.0
62	1646.0
63	1409.0
64	1245.5
65	1082.0
66	910.0
67	738.0
68	600.5
69	463.0
70	410.5
71	358.0
72	284.0
73	210.0
74	187.0
75	127.0
76	90.0
77	65.5
78	41.0
79	31.5
80	22.0
81	17.0
82	12.0
83	7.0
84	2.0
85	2.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	101668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.91690600779006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46706925225648	49.62721800369832
2	11.30738055640773	13.776212770980054
3	3.658792566160205	6.686469685643467
4	1.3546897453699966	3.3009403155368453
5	0.6103369770558507	1.8589920132194986
6	0.33261750601456413	1.2157217610260849
7	0.2502704535546478	1.0671991187000827
8	0.17761128961942743	0.8655624188535231
9	0.11625466229635252	0.6373686902466853
>10	0.6297127541052427	7.018924341975843
>50	0.06135662732307494	2.6281622536097884
>100	0.022605073224290766	2.8514380139276865
>500	0.008073240437246701	3.3747098398709525
>1k	0.0032292961748986806	5.0910807727111775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3725	3.6638863752606525	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	1451	1.4271943974505252	No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	884	0.8694967934846757	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	732	0.7199905575008853	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	692	0.6806468111893614	No Hit
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	598	0.5881890073572805	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	525	0.5163866703387496	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	355	0.34917574851477357	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	352	0.3462249675414093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	334	0.32852028170122355	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	289	0.2842585671007593	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	247	0.24294763347365939	No Hit
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	209	0.20557107447771178	No Hit
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	175	0.17212889011291654	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	172	0.16917810913955228	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.15048982964157848	No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	135	0.13278514380139278	No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	135	0.13278514380139278	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC	124	0.12196561356572373	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	115	0.11311327064563088	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	104	0.10229374040996184	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.835936577880947E-4	0.0	0.0	1.0131014675217374	0.0
2	9.835936577880947E-4	0.0	0.0	3.1661879844198766	0.0
3	9.835936577880947E-4	0.0	0.0	4.308140221111854	0.0
4	9.835936577880947E-4	0.0	0.0	5.869103356021561	0.0
5	9.835936577880947E-4	0.0	0.0	8.917260101506866	0.0
6	9.835936577880947E-4	0.0	0.0	10.837234921509227	0.0
7	9.835936577880947E-4	0.0	0.0	12.831962859503482	0.0
8	9.835936577880947E-4	0.0	0.0	15.551599323287563	0.0
9	9.835936577880947E-4	0.0	0.0	16.88928669787937	0.0
10	9.835936577880947E-4	0.0	0.0	19.90596844631546	0.0
11	9.835936577880947E-4	0.0	0.0	25.843923358382185	0.0
12	9.835936577880947E-4	0.0	0.0	30.692056497619703	0.0
13	9.835936577880947E-4	0.0	0.0	32.42023055435339	0.0
14	9.835936577880947E-4	0.0	0.0	33.113664083093994	0.0
15	9.835936577880947E-4	0.0	0.0	34.097257740882085	0.0
16	9.835936577880947E-4	0.0	0.0	36.25722941338474	0.0
17	9.835936577880947E-4	0.0	0.0	38.82440886021167	0.0
18	9.835936577880947E-4	0.0	0.0	41.12011645748908	0.0
19	9.835936577880947E-4	0.0	0.0	42.594523350513434	0.0
20	9.835936577880947E-4	0.0	0.0	43.701066215525046	0.0
21	9.835936577880947E-4	0.0	0.0	44.922689538497856	0.0
22	9.835936577880947E-4	0.0	0.0	45.918086320179405	0.0
23	9.835936577880947E-4	0.0	0.0	46.69905968446315	0.0
24	9.835936577880947E-4	0.0	0.0	47.17315182751702	0.0
25	9.835936577880947E-4	0.0	0.0	47.52036038871621	0.0
26	9.835936577880947E-4	0.0	0.0	47.797733800212455	0.0
27	9.835936577880947E-4	0.0	0.0	47.99740331274344	0.0
28	9.835936577880947E-4	0.0	0.0	48.227564228665855	0.0
29	9.835936577880947E-4	0.0	0.0	48.42723374119684	0.0
30	9.835936577880947E-4	0.0	0.0	48.61510012983436	0.0
31	9.835936577880947E-4	0.0	0.0	48.781327458000554	0.0
32	9.835936577880947E-4	0.0	0.0	48.9426368178778	0.0
33	9.835936577880947E-4	0.0	0.0	49.099028209466105	0.0
34	9.835936577880947E-4	0.0	0.0	49.25738678836999	0.0
35	9.835936577880947E-4	0.0	0.0	49.422630522878386	0.0
36	9.835936577880947E-4	0.0	0.0	49.56820238423103	0.0
37	9.835936577880947E-4	0.0	0.0	49.69508596608569	0.0
38	9.835936577880947E-4	0.0	0.0	49.84262501475391	0.0
39	9.835936577880947E-4	0.0	0.0	49.970492190266356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGGA	25	3.865679E-5	45.000004	4
TGTTCGG	30	2.1459618E-6	45.000004	2
CGGGATT	25	3.865679E-5	45.000004	6
TGGAATG	25	3.865679E-5	45.000004	6
TGGGTGA	30	2.1459618E-6	45.000004	6
TGGGTAC	25	3.865679E-5	45.000004	6
GGTGATA	20	7.0029486E-4	45.0	8
TTGTTCG	20	7.0029486E-4	45.0	1
GGGTCTA	20	7.0029486E-4	45.0	7
CCTATGT	20	7.0029486E-4	45.0	10
CGGGGTT	20	7.0029486E-4	45.0	6
GTTCTTG	45	3.765308E-10	45.0	1
TGTTCAT	20	7.0029486E-4	45.0	14
GGCCCAC	20	7.0029486E-4	45.0	8
GGCCTAT	20	7.0029486E-4	45.0	8
TGCTCGG	20	7.0029486E-4	45.0	2
ATGCTGG	20	7.0029486E-4	45.0	2
CCCTGGA	20	7.0029486E-4	45.0	3
TCGTTGA	20	7.0029486E-4	45.0	24
TGGGCGA	80	0.0	45.0	6
>>END_MODULE