FastQCFastQC Report
Sat 14 Jan 2017
SRR2933707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103413
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36473.526635916180751No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC14251.3779698877317166No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT9800.9476564841944436TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC7470.7223463200951524TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC6590.6372506358001412No Hit
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC5730.5540889443300165TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC5170.4999371452331912TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG3560.3442507228298183No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC3490.3374817479427151No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC3010.29106592014543625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.27656097395878665No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG2530.2446500923481574No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT2080.20113525378820846No Hit
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG1990.19243228607621865No Hit
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG1760.17019136859002254No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.14988444392871303No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG1320.12764352644251692No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC1300.12570953361763026No Hit
TTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG1300.12570953361763026No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG1180.11410557666831056No Hit
TTTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC1150.11120458743098063No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACT1060.10250161971899084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATTA302.146322E-645.0000045
AAACACG302.146322E-645.00000440
CACGTGA302.146322E-645.00000443
CACGACC302.146322E-645.00000427
CCATTAC302.146322E-645.0000046
CTAATCC302.146322E-645.00000411
GGGACCC302.146322E-645.0000047
ACGACCA302.146322E-645.00000428
TGGGAAG453.783498E-1045.0000046
AGACACG302.146322E-645.00000424
CGACCAA302.146322E-645.00000429
AACACGT302.146322E-645.00000441
GACTAAT302.146322E-645.0000049
GGAGATC207.00351E-445.08
ATCCTAG207.00351E-445.014
AATCCTA207.00351E-445.013
GGATGAC253.8661456E-545.08
GGCACCG253.8661456E-545.08
CCCGGGA207.00351E-445.04
GGACTAA351.1976408E-745.08
Produced by FastQC (version 0.11.5)