FastQCFastQC Report
Sat 14 Jan 2017
SRR2933706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733179
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1432505.241149591739144No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC58530.2141462377692789No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC34480.12615346451878928No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA31120.11386008746591422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG453.8562575E-1045.0000041
CGAACGA253.8922233E-544.99999622
CGTTTTT843250.044.4556771
GTTTTTT947450.039.9963072
TGCGACG301.1403335E-437.5000041
CGATCGC800.036.562510
TGGGCGA44300.036.111746
TTGCGCG3500.036.0000041
GCCGTCG250.00210761635.9999961
TTGGGCG35950.035.9248965
GGCCGAT9000.035.7500048
TTGGGAC51400.035.5447465
CGCGGGT2550.035.2941174
CGAATAT1600.035.1562514
CTTTGCG6150.035.1219521
ACGGGCG1550.034.8387075
CACGACC6150.034.75609627
ACACGAC6200.034.47580726
GGGCGAT68200.034.409827
GCCGATT5300.034.386799
Produced by FastQC (version 0.11.5)