##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933706.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2733179
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60897694589341	34.0	31.0	34.0	31.0	34.0
2	32.81248392439719	34.0	31.0	34.0	31.0	34.0
3	32.82791577134172	34.0	31.0	34.0	31.0	34.0
4	36.296115622138174	37.0	37.0	37.0	35.0	37.0
5	36.30762566227825	37.0	37.0	37.0	35.0	37.0
6	36.286492395851134	37.0	37.0	37.0	35.0	37.0
7	36.36122149335993	37.0	37.0	37.0	35.0	37.0
8	36.29134645041543	37.0	37.0	37.0	35.0	37.0
9	38.13133936708866	39.0	39.0	39.0	37.0	39.0
10	37.55840506604214	39.0	38.0	39.0	35.0	39.0
11	37.47647556197381	39.0	37.0	39.0	34.0	39.0
12	37.54704540024638	39.0	37.0	39.0	35.0	39.0
13	37.60583152438973	39.0	37.0	39.0	35.0	39.0
14	39.00271625092978	40.0	38.0	41.0	35.0	41.0
15	39.03903513088605	40.0	38.0	41.0	35.0	41.0
16	39.04549866657105	40.0	38.0	41.0	35.0	41.0
17	38.98276878316422	40.0	38.0	41.0	35.0	41.0
18	38.436867106032935	39.0	38.0	41.0	35.0	41.0
19	37.877533085099806	38.0	37.0	41.0	35.0	41.0
20	37.25750783245444	38.0	35.0	41.0	34.0	41.0
21	37.1680804660068	38.0	35.0	40.0	34.0	41.0
22	37.16311664914738	38.0	35.0	40.0	34.0	41.0
23	37.081232513494356	38.0	35.0	40.0	34.0	41.0
24	36.98091197100519	38.0	35.0	40.0	33.0	41.0
25	36.92119286735336	38.0	35.0	40.0	33.0	41.0
26	36.88635285138661	38.0	35.0	40.0	33.0	41.0
27	36.82253851650404	38.0	35.0	40.0	33.0	41.0
28	36.664140548423646	38.0	35.0	40.0	33.0	41.0
29	36.50805746714723	38.0	35.0	40.0	33.0	41.0
30	36.27527871390787	38.0	35.0	40.0	33.0	41.0
31	35.81025831092658	38.0	35.0	40.0	31.0	41.0
32	34.99140195354933	38.0	35.0	40.0	23.0	41.0
33	33.919080674921034	38.0	34.0	40.0	16.0	41.0
34	32.955785186407475	38.0	33.0	40.0	10.0	41.0
35	32.323957925917036	38.0	31.0	40.0	8.0	41.0
36	31.976020963134868	38.0	30.0	40.0	7.0	41.0
37	31.754744200800605	38.0	29.0	40.0	7.0	41.0
38	31.60464389635659	37.0	28.0	40.0	7.0	41.0
39	31.474439105525104	37.0	27.0	40.0	7.0	41.0
40	31.322039646872746	37.0	25.0	40.0	7.0	41.0
41	31.158872872943924	37.0	24.0	40.0	7.0	41.0
42	31.00548884650438	37.0	23.0	40.0	7.0	41.0
43	30.83665321590719	37.0	23.0	40.0	7.0	41.0
44	30.673680721240725	37.0	23.0	40.0	7.0	41.0
45	30.641132907870286	36.0	23.0	40.0	7.0	41.0
46	30.519650194882956	36.0	23.0	40.0	7.0	41.0
47	30.42814941868059	36.0	23.0	40.0	7.0	41.0
48	30.342436042425323	36.0	23.0	40.0	7.0	41.0
49	30.29404184650914	36.0	22.0	40.0	7.0	41.0
50	30.19504430555042	35.0	22.0	40.0	7.0	41.0
51	29.436006569639236	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	7.0
11	11.0
12	11.0
13	17.0
14	40.0
15	67.0
16	173.0
17	411.0
18	974.0
19	2149.0
20	3866.0
21	6378.0
22	10328.0
23	18375.0
24	37752.0
25	82525.0
26	137903.0
27	147958.0
28	120589.0
29	91481.0
30	74524.0
31	68601.0
32	68748.0
33	78502.0
34	111556.0
35	147285.0
36	189216.0
37	233745.0
38	456494.0
39	643264.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.88579489305311	3.122956820610725	39.79574700376375	40.19550128257242
2	44.46119335762495	3.0767834817990334	38.53805403890488	13.923969121671137
3	14.395032304872824	3.256464358902216	68.18887456694202	14.159628769282948
4	12.061961547341028	3.3826178234210054	68.37268982382786	16.182730805410113
5	12.128587260475806	4.322658706217193	67.84773335372472	15.701020679582273
6	14.583860039902252	4.9665243293615235	68.74401566820175	11.70559996253447
7	60.12222397435367	1.383151268175264	35.88861175942007	2.6060129980509874
8	62.77744706804786	1.5016945468994163	33.81926320961781	1.9015951754349054
9	58.265704514779316	4.056046091382965	35.05364998048061	2.62459941335712
10	24.495212351624243	22.61747218166099	42.01418933776383	10.87312612895094
11	17.91745070483858	19.44600042660945	49.00977945462043	13.626769413931541
12	14.257061099913324	18.463591297898894	52.50197663599786	14.77737096618992
13	15.366940840684054	18.101485486314655	53.552474975111394	12.979098697889894
14	15.296217335198317	18.84797885539147	52.51518469884336	13.340619110566854
15	14.274184017951258	19.300236098696793	52.87758320988124	13.547996673470708
16	15.749242914569445	19.077601576771958	51.67484456744326	13.498310941215339
17	15.94816878074945	18.95360677072376	51.355472876090445	13.742751572436346
18	16.69630858425299	17.784455390590956	51.50965231329525	14.009583711860804
19	16.34528876447536	19.78399512070011	50.058265484990194	13.812450629834345
20	17.364212150027495	19.9894335497236	49.428193323598634	13.218160976650267
21	17.223533475121826	20.59630927941419	49.53630918428687	12.643848061177113
22	16.96851907613808	18.72577683349682	49.7889454002098	14.51675869015531
23	14.898951001745587	20.850408992605317	49.560456889212155	14.69018311643694
24	14.23964548242175	20.68583872479629	50.37214906158726	14.702366731194699
25	14.792005938871913	21.810133913658785	49.09806492732455	14.299795220144748
26	15.1023405345936	21.5247519463599	48.87850374966294	14.494403769383565
27	14.889987080977866	20.82417580407284	49.67654881001208	14.609288304937218
28	14.366055058962477	20.704425140102423	49.77584709965941	15.153672701275694
29	15.176137384342555	19.97929883114132	49.41004595747297	15.434517827043162
30	15.867383731544843	19.669622809190322	49.39621590828848	15.066777550976354
31	15.890653338109212	20.627664708385364	47.755964757522285	15.725717195983139
32	15.552000070247868	21.000966274071327	47.35013696505059	16.096896690630214
33	16.521969472178736	22.07850272521485	44.27701222642205	17.122515576184362
34	16.339544537697677	23.492277673727187	42.70430147458326	17.463876313991875
35	17.00141117724086	24.425659643953068	40.37906042743633	18.19386875136974
36	17.53759998887742	25.7050855432447	38.522138506113215	18.23517596176467
37	17.99168660376799	25.801090964038583	38.06688109340808	18.140341338785348
38	18.376952259621486	26.455127893196895	37.88226091302472	17.285658934156892
39	18.88935924064981	26.210906786566117	36.7995656340108	18.100168338773276
40	19.117079415581635	26.153903567969756	36.89476613130717	17.834250885141444
41	18.4768359481761	25.91513398866302	36.91927971054951	18.688750352611372
42	19.10573731175309	26.107035067955664	36.879253060264254	17.907974560026986
43	18.79456120510219	26.03411631656763	37.101668057598864	18.069654420731318
44	18.72087411764835	26.468701830359446	36.37588317486707	18.434540877125137
45	18.899311022073565	25.796590709938865	36.1331621529362	19.170936115051372
46	18.66339526243982	25.998772857540615	36.196787696671166	19.141044183348402
47	17.962782532721057	26.532656660979757	36.66580198369737	18.83875882260181
48	18.300886989106825	25.946050368453733	36.82671350833589	18.92634913410355
49	19.039770172389	25.31714900487674	36.6418006284989	19.001280194235356
50	18.12523072949119	25.412422677036524	36.62658025690963	19.835766336562664
51	17.773369398784347	24.937261701483877	36.27267003002731	21.016698869704474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1116.0
1	2862.5
2	4609.0
3	114582.0
4	224555.0
5	148070.0
6	71585.0
7	69951.5
8	68318.0
9	68480.0
10	68642.0
11	68087.5
12	67533.0
13	65856.0
14	64179.0
15	61291.0
16	58403.0
17	55917.0
18	53431.0
19	50309.5
20	47188.0
21	45150.5
22	43113.0
23	41425.5
24	39738.0
25	40037.5
26	41551.5
27	42766.0
28	45241.5
29	47717.0
30	51780.0
31	55843.0
32	61274.0
33	66705.0
34	73365.5
35	80026.0
36	85970.0
37	91914.0
38	99680.5
39	107447.0
40	112242.0
41	117037.0
42	125102.5
43	133168.0
44	140369.5
45	147571.0
46	155420.5
47	163270.0
48	168498.0
49	173726.0
50	167675.0
51	161624.0
52	146753.5
53	131883.0
54	117624.5
55	103366.0
56	91229.5
57	79093.0
58	69306.5
59	59520.0
60	51149.5
61	42779.0
62	36047.5
63	29316.0
64	23527.5
65	17739.0
66	13834.5
67	9930.0
68	7861.5
69	5793.0
70	4709.0
71	3625.0
72	3224.0
73	2823.0
74	2701.5
75	2214.0
76	1848.0
77	1372.5
78	897.0
79	574.0
80	251.0
81	187.0
82	123.0
83	72.0
84	21.0
85	18.5
86	16.0
87	11.5
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2733179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.2988168222172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40552763555584	30.387535455679014
2	8.340045524769204	6.7218793378325286
3	3.8042283915913404	4.599177093078522
4	2.4539630280480837	3.9556722622321265
5	1.8010398390498066	3.628988728169185
6	1.323623645127123	3.200428008992002
7	1.056583381185289	2.980534209504939
8	0.8532588084089406	2.750825633761217
9	0.7409203612365868	2.687239252559179
>10	4.1091855995775335	26.16302578431453
>50	0.06161179864285929	1.7217841020241103
>100	0.04454347036141026	3.567035490176896
>500	0.003554535689817293	0.9629641666583776
>1k	0.00173169687452233	1.19648793550257
>5k	9.114194076433316E-5	0.2149755613487396
>10k+	9.114194076433316E-5	5.261446978166231
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143250	5.241149591739144	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	5853	0.2141462377692789	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	3448	0.12615346451878928	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	3112	0.11386008746591422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.658743170498529E-4	0.0	0.0	0.060881486357095525	0.0
2	3.658743170498529E-4	0.0	0.0	0.17426593721084496	0.0
3	3.658743170498529E-4	0.0	0.0	0.28746745090606945	0.0
4	3.658743170498529E-4	0.0	0.0	0.4280729509483279	0.0
5	3.658743170498529E-4	0.0	0.0	0.7332853062313153	0.0
6	3.658743170498529E-4	0.0	0.0	1.28125527087688	0.0
7	3.658743170498529E-4	0.0	0.0	1.808297224587193	0.0
8	3.658743170498529E-4	0.0	0.0	2.557754175632112	0.0
9	3.658743170498529E-4	0.0	0.0	3.0688440091190516	0.0
10	4.0246174875483825E-4	0.0	0.0	3.7009284792543773	0.0
11	4.0246174875483825E-4	0.0	0.0	4.573246025964637	0.0
12	4.0246174875483825E-4	0.0	0.0	5.2811396545926925	0.0
13	4.390491804598235E-4	0.0	0.0	5.61196321206917	0.0
14	4.756366121648088E-4	0.0	0.0	5.787802408843328	0.0
15	5.122240438697941E-4	0.0	0.0	5.930493392492771	0.0
16	5.122240438697941E-4	0.0	0.0	6.153420613871247	0.0
17	5.122240438697941E-4	0.0	0.0	6.44333942270155	0.0
18	5.122240438697941E-4	0.0	0.0	6.752320283450151	0.0
19	5.122240438697941E-4	0.0	0.0	6.9592953846052525	0.0
20	5.122240438697941E-4	0.0	0.0	7.1608189584363116	0.0
21	5.488114755747794E-4	0.0	0.0	7.385538963968331	0.0
22	5.488114755747794E-4	0.0	0.0	7.608246660756577	0.0
23	5.488114755747794E-4	0.0	0.0	7.823124647159956	0.0
24	5.488114755747794E-4	0.0	0.0	7.999183368524345	0.0
25	5.488114755747794E-4	0.0	0.0	8.157643535238636	0.0
26	5.853989072797647E-4	0.0	0.0	8.312847420531183	0.0
27	5.853989072797647E-4	0.0	0.0	8.477746975225552	0.0
28	5.853989072797647E-4	0.0	0.0	8.655196018994731	0.0
29	5.853989072797647E-4	0.0	0.0	8.843987166592456	0.0
30	5.853989072797647E-4	0.0	0.0	9.069621857917099	0.0
31	5.853989072797647E-4	0.0	0.0	9.25354687709806	0.0
32	6.2198633898475E-4	0.0	0.0	9.452984967321935	0.0
33	6.2198633898475E-4	0.0	0.0	9.645910494702322	0.0
34	6.2198633898475E-4	0.0	0.0	9.837848161426676	0.0
35	6.585737706897353E-4	0.0	0.0	10.05429940739337	0.0
36	7.317486340997058E-4	0.0	0.0	10.253993609639178	0.0
37	7.317486340997058E-4	0.0	0.0	10.465688489484224	0.0
38	7.683360658046911E-4	0.0	0.0	10.693298902120937	0.0
39	7.683360658046911E-4	0.0	0.0	10.989218049750859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTCG	45	3.8562575E-10	45.000004	1
CGAACGA	25	3.8922233E-5	44.999996	22
CGTTTTT	84325	0.0	44.455677	1
GTTTTTT	94745	0.0	39.996307	2
TGCGACG	30	1.1403335E-4	37.500004	1
CGATCGC	80	0.0	36.5625	10
TGGGCGA	4430	0.0	36.11174	6
TTGCGCG	350	0.0	36.000004	1
GCCGTCG	25	0.002107616	35.999996	1
TTGGGCG	3595	0.0	35.924896	5
GGCCGAT	900	0.0	35.750004	8
TTGGGAC	5140	0.0	35.544746	5
CGCGGGT	255	0.0	35.294117	4
CGAATAT	160	0.0	35.15625	14
CTTTGCG	615	0.0	35.121952	1
ACGGGCG	155	0.0	34.838707	5
CACGACC	615	0.0	34.756096	27
ACACGAC	620	0.0	34.475807	26
GGGCGAT	6820	0.0	34.40982	7
GCCGATT	530	0.0	34.38679	9
>>END_MODULE