FastQCFastQC Report
Sat 14 Jan 2017
SRR2933705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766093
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1415925.118844521858087No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC58570.2117426999019917No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC34880.12609843559128345No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA30400.10990230624928372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT835550.044.480281
GTTTTTT944000.039.8731462
TACGGGA3850.039.155844
GCTTACG659.094947E-1238.076921
CGAACGA250.002107620535.99999622
TTGGGAC54450.035.6198355
TGGGCGA43300.035.5946856
TTGGGCG34100.035.3005875
CTGCGCG2250.035.01
CGTTTGG13200.034.7727282
TTCGTCG1300.034.6153831
GGGCGAT67150.034.3782587
CGGGATA8600.034.2732546
GGACCGA11500.034.2391328
TCTTGCG6250.034.21
AGGCACG6450.034.18604710
GCGAGAC8100.034.16666421
TCTGGGA60000.034.16254
TATCCGG1450.034.1379282
ACGGGCG1650.034.0909085
Produced by FastQC (version 0.11.5)