##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933705.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2766093
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52584023747575	34.0	31.0	34.0	31.0	34.0
2	32.75043644591849	34.0	31.0	34.0	31.0	34.0
3	32.77552707013105	34.0	31.0	34.0	31.0	34.0
4	36.217326387796795	37.0	37.0	37.0	35.0	37.0
5	36.27898194312339	37.0	37.0	37.0	35.0	37.0
6	36.27293189346851	37.0	37.0	37.0	35.0	37.0
7	36.28526770430351	37.0	37.0	37.0	35.0	37.0
8	36.24748661740585	37.0	37.0	37.0	35.0	37.0
9	38.097655791038115	39.0	39.0	39.0	37.0	39.0
10	37.56264304923949	39.0	38.0	39.0	35.0	39.0
11	37.47419338395347	39.0	37.0	39.0	34.0	39.0
12	37.523171491341756	39.0	37.0	39.0	35.0	39.0
13	37.59576413374388	39.0	37.0	39.0	35.0	39.0
14	39.0130982580846	40.0	38.0	41.0	35.0	41.0
15	39.03801932906811	40.0	39.0	41.0	35.0	41.0
16	39.093033748322995	41.0	39.0	41.0	35.0	41.0
17	39.04980129012293	40.0	39.0	41.0	35.0	41.0
18	38.54744290954787	39.0	38.0	41.0	35.0	41.0
19	38.0184277968962	38.0	37.0	41.0	35.0	41.0
20	37.36209411614143	38.0	35.0	41.0	34.0	41.0
21	37.29919167576795	38.0	35.0	41.0	34.0	41.0
22	37.28017676918311	38.0	35.0	41.0	34.0	41.0
23	37.142027039582544	38.0	35.0	41.0	34.0	41.0
24	37.05163853854516	38.0	35.0	41.0	34.0	41.0
25	37.00135678735314	38.0	35.0	40.0	33.0	41.0
26	36.9949531704104	38.0	35.0	40.0	34.0	41.0
27	36.935135586547524	38.0	35.0	40.0	33.0	41.0
28	36.83340075695214	38.0	35.0	40.0	33.0	41.0
29	36.63259008283525	38.0	35.0	40.0	33.0	41.0
30	36.323325716091254	38.0	35.0	40.0	33.0	41.0
31	35.85307435433299	38.0	35.0	40.0	31.0	41.0
32	35.11334506829669	38.0	35.0	41.0	23.0	41.0
33	33.991722621039855	38.0	34.0	41.0	16.0	41.0
34	33.06121450001862	38.0	33.0	41.0	10.0	41.0
35	32.478274953155946	38.0	31.0	41.0	8.0	41.0
36	32.12102774563256	38.0	31.0	41.0	7.0	41.0
37	31.937214692347656	38.0	30.0	41.0	7.0	41.0
38	31.772012365455534	38.0	29.0	41.0	7.0	41.0
39	31.673515677166314	38.0	29.0	41.0	7.0	41.0
40	31.51749525413643	38.0	27.0	40.0	7.0	41.0
41	31.364368804664196	37.0	25.0	40.0	7.0	41.0
42	31.147052900968983	37.0	24.0	40.0	7.0	41.0
43	31.020082838863335	37.0	23.0	40.0	7.0	41.0
44	30.8638332116816	37.0	23.0	40.0	7.0	41.0
45	30.814002999899134	37.0	23.0	40.0	7.0	41.0
46	30.673877920952044	36.0	23.0	40.0	7.0	41.0
47	30.63957755577994	36.0	23.0	40.0	7.0	41.0
48	30.51720350689583	36.0	23.0	40.0	7.0	41.0
49	30.457210223951254	36.0	23.0	40.0	7.0	41.0
50	30.31095013797439	36.0	22.0	40.0	7.0	41.0
51	29.648097153638723	35.0	20.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	12.0
11	15.0
12	20.0
13	22.0
14	52.0
15	76.0
16	170.0
17	421.0
18	1011.0
19	2081.0
20	3723.0
21	6261.0
22	10200.0
23	18263.0
24	37053.0
25	81743.0
26	136719.0
27	147794.0
28	120964.0
29	92161.0
30	74925.0
31	68313.0
32	68668.0
33	77346.0
34	111291.0
35	146507.0
36	187652.0
37	227738.0
38	447465.0
39	697036.0
40	371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.015190740152264	3.261929371138281	39.88951203014505	39.83336785856441
2	44.24193257421207	3.1189840688653634	38.6220564529103	14.017026904012267
3	14.573298873175991	3.338282552321994	67.82613599759661	14.262282576905406
4	12.277967515915048	3.450643199632116	67.96568300487365	16.305706279579173
5	12.230644450493891	4.351589046355275	67.65228067169107	15.765485831459753
6	14.65973848312403	5.044479704767699	68.45106075609172	11.844721056016555
7	60.36084108524189	1.428874589538385	35.53846526490613	2.6718190603135903
8	63.10004761228202	1.5030224941822274	33.48361750671434	1.9133123868214121
9	58.573880198532734	4.089197290185109	34.67775667701701	2.6591658342651527
10	24.62802226823176	22.70386425908312	41.68663164976738	10.98148182291774
11	18.06768608286128	19.58394023628273	48.66965788930452	13.678715791551477
12	14.369075804754214	18.638563490092345	52.179807403438716	14.812553301714729
13	15.458518567524665	18.24121604009699	53.27384147966102	13.026423912717325
14	15.366113865296649	18.966968934160928	52.21755016913748	13.449367031404947
15	14.390983961855222	19.393418804067686	52.62050119066857	13.59509604340852
16	15.860131962302063	19.205283408764636	51.37527913920464	13.559305489728654
17	16.076212911134945	18.99368531716034	51.16064427334873	13.769457498355983
18	16.829332925537933	17.904025641943345	51.22398270773976	14.042658724778956
19	16.453423655676076	19.835667130497782	49.810400445682774	13.900508768143371
20	17.553169759657393	20.082730407112127	49.13399513320774	13.230104700022741
21	17.368938788392146	20.679673460002974	49.25383925992365	12.697548491681227
22	17.178489660325955	18.807429829727347	49.48839391878725	14.525686591159445
23	15.059616578329074	20.949331783132383	49.278964951648405	14.712086686890139
24	14.38610343180797	20.74973618023689	50.139818147835236	14.724342240119908
25	14.962512106425924	21.91498984307469	48.79944383648706	14.323054214012327
26	15.242148402096387	21.638064952986035	48.577325491225345	14.54246115369223
27	14.979612037628526	20.983857014207405	49.397724516131596	14.638806432032473
28	14.488160737907222	20.81846127371712	49.58282313718303	15.11055485119264
29	15.314524855093447	20.069426443724055	49.19324838318885	15.422800317993646
30	15.94559546624065	19.75974777420716	49.183884995913004	15.110771763639184
31	16.016055859293235	20.732455488662165	47.498583742484435	15.752904909560165
32	15.706304885627489	21.07731012659372	47.082220301342005	16.13416468643679
33	16.605587736927138	22.19126399582371	44.00365425168279	17.199494015566362
34	16.494709324668406	23.610341373193165	42.451103415539535	17.443845886598897
35	17.040641800546837	24.551741391196895	40.22138807335834	18.18622873489792
36	17.59300934567276	25.7825387649656	38.46258965262556	18.161862236736077
37	18.033413916307225	25.885572177074305	37.97453664790012	18.106477258718343
38	18.437087979326797	26.567761821457196	37.72740106713693	17.267749132079075
39	18.954424164335762	26.287727852968068	36.718722038629934	18.039125944066235
40	19.206151058550816	26.25280494907438	36.7840488371143	17.75699515526051
41	18.51344116051051	26.030216626845153	36.830540404823694	18.625801807820633
42	19.0945496048036	26.235054280532143	36.827033653604566	17.843362461059698
43	18.84701635122174	26.06344038324091	37.065781953101364	18.023761312435987
44	18.805730682229413	26.46592142780449	36.33099104043139	18.397356849534706
45	18.963172966346395	25.857156646576957	36.04726956035101	19.132400826725636
46	18.682669020889755	26.119223034077304	36.15670188963278	19.041406055400163
47	18.048308570969958	26.674012768189648	36.5796450083204	18.69803365252
48	18.383185236360454	26.095073448362005	36.69927222258977	18.822469092687776
49	19.07958264599202	25.46407514136365	36.5510125653765	18.905329647267823
50	18.132795968899092	25.51761636358575	36.5928405154852	19.75674715202996
51	17.844627783664542	25.038818289912886	36.27061707614313	20.845936850279436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1062.0
1	2936.5
2	4811.0
3	114599.0
4	224387.0
5	148791.0
6	73195.0
7	70944.5
8	68694.0
9	68576.5
10	68459.0
11	68217.5
12	67976.0
13	66168.5
14	64361.0
15	61511.0
16	58661.0
17	55867.5
18	53074.0
19	49999.5
20	46925.0
21	44804.5
22	42684.0
23	41208.5
24	39733.0
25	40070.5
26	41530.5
27	42653.0
28	45408.0
29	48163.0
30	52499.0
31	56835.0
32	62193.0
33	67551.0
34	74549.5
35	81548.0
36	87913.0
37	94278.0
38	102087.5
39	109897.0
40	114489.0
41	119081.0
42	127701.5
43	136322.0
44	142923.0
45	149524.0
46	157470.5
47	165417.0
48	170308.0
49	175199.0
50	169812.5
51	164426.0
52	149268.0
53	134110.0
54	119670.5
55	105231.0
56	93207.0
57	81183.0
58	70718.0
59	60253.0
60	51822.0
61	43391.0
62	36680.0
63	29969.0
64	23937.0
65	17905.0
66	14062.5
67	10220.0
68	8085.5
69	5951.0
70	4787.0
71	3623.0
72	3262.0
73	2901.0
74	2786.0
75	2311.5
76	1952.0
77	1426.5
78	901.0
79	615.0
80	329.0
81	234.0
82	139.0
83	79.0
84	19.0
85	13.0
86	7.0
87	5.0
88	3.0
89	4.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2766093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.95134970235873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19954417169092	30.04323286661196
2	8.217300895447572	6.565845233670629
3	3.8528813992024262	4.61783436423748
4	2.521909665119749	4.030147797958299
5	1.7557801191888716	3.5072892771081854
6	1.4258655381621224	3.4179151646194126
7	1.106346927534955	3.094003709585539
8	0.9000054055736811	2.876514455366987
9	0.7418388141403829	2.6673715697854994
>10	4.165663544778824	26.406859555468916
>50	0.06319673050429518	1.760142909807821
>100	0.04446043318943371	3.577758698555173
>500	0.003470903644511746	0.9455284286890322
>1k	0.001552772683065079	1.1089368769768717
>5k	9.133956959206347E-5	0.21373007629252486
>10k+	9.133956959206347E-5	5.166889015265696
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141592	5.118844521858087	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	5857	0.2117426999019917	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	3488	0.12609843559128345	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	3040	0.10990230624928372	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5306452096874547E-4	0.0	0.0	0.05972322694862393	0.0
2	2.5306452096874547E-4	0.0	0.0	0.1659018695322247	0.0
3	2.5306452096874547E-4	0.0	0.0	0.2729120098275799	0.0
4	2.5306452096874547E-4	0.0	0.0	0.4137243397094747	0.0
5	2.5306452096874547E-4	0.0	0.0	0.7144372947691925	0.0
6	2.5306452096874547E-4	0.0	0.0	1.247535784227067	0.0
7	2.5306452096874547E-4	0.0	0.0	1.7677641351899593	0.0
8	2.5306452096874547E-4	0.0	0.0	2.4875157848994953	0.0
9	2.5306452096874547E-4	0.0	0.0	2.999320702521571	0.0
10	2.8921659539285195E-4	0.0	0.0	3.616725829536462	0.0
11	2.8921659539285195E-4	0.0	0.0	4.46423891026079	0.0
12	3.2536866981695844E-4	0.0	0.0	5.156008854366068	0.0
13	3.2536866981695844E-4	0.0	0.0	5.4797145287595175	0.0
14	3.2536866981695844E-4	0.0	0.0	5.649701582701666	0.0
15	3.6152074424106493E-4	0.0	0.0	5.791815387262829	0.0
16	3.6152074424106493E-4	0.0	0.0	6.007860184021289	0.0
17	3.976728186651714E-4	0.0	0.0	6.27954302331845	0.0
18	3.976728186651714E-4	0.0	0.0	6.5797136972618055	0.0
19	3.976728186651714E-4	0.0	0.0	6.782924507599708	0.0
20	4.699769675133844E-4	0.0	0.0	6.985376124374705	0.0
21	4.699769675133844E-4	0.0	0.0	7.202360875068192	0.0
22	5.061290419374909E-4	0.0	0.0	7.420249427622282	0.0
23	5.061290419374909E-4	0.0	0.0	7.63108832566367	0.0
24	5.061290419374909E-4	0.0	0.0	7.797640932535529	0.0
25	5.061290419374909E-4	0.0	0.0	7.956348539257357	0.0
26	5.061290419374909E-4	0.0	0.0	8.113393150555675	0.0
27	5.061290419374909E-4	0.0	0.0	8.27481216285931	0.0
28	5.422811163615974E-4	0.0	0.0	8.444835368875884	0.0
29	5.422811163615974E-4	0.0	0.0	8.630837791787911	0.0
30	5.422811163615974E-4	0.0	0.0	8.855450630184885	0.0
31	5.784331907857039E-4	0.0	0.0	9.035524112891359	0.0
32	6.145852652098103E-4	0.0	0.0	9.234866651265882	0.0
33	6.507373396339169E-4	0.0	0.0	9.427593360020795	0.0
34	6.868894140580233E-4	0.0	0.0	9.615294930430755	0.0
35	6.868894140580233E-4	0.0	0.0	9.829568275542435	0.0
36	6.868894140580233E-4	0.0	0.0	10.022367288446194	0.0
37	7.230414884821299E-4	0.0	0.0	10.237833652013869	0.0
38	7.591935629062363E-4	0.0	0.0	10.466423218597495	0.0
39	7.953456373303428E-4	0.0	0.0	10.758568132018699	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	83555	0.0	44.48028	1
GTTTTTT	94400	0.0	39.873146	2
TACGGGA	385	0.0	39.15584	4
GCTTACG	65	9.094947E-12	38.07692	1
CGAACGA	25	0.0021076205	35.999996	22
TTGGGAC	5445	0.0	35.619835	5
TGGGCGA	4330	0.0	35.594685	6
TTGGGCG	3410	0.0	35.300587	5
CTGCGCG	225	0.0	35.0	1
CGTTTGG	1320	0.0	34.772728	2
TTCGTCG	130	0.0	34.615383	1
GGGCGAT	6715	0.0	34.378258	7
CGGGATA	860	0.0	34.273254	6
GGACCGA	1150	0.0	34.239132	8
TCTTGCG	625	0.0	34.2	1
AGGCACG	645	0.0	34.186047	10
GCGAGAC	810	0.0	34.166664	21
TCTGGGA	6000	0.0	34.1625	4
TATCCGG	145	0.0	34.137928	2
ACGGGCG	165	0.0	34.090908	5
>>END_MODULE