##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	905989
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.088087162206165	33.0	31.0	34.0	30.0	34.0
2	32.29804445749341	34.0	31.0	34.0	30.0	34.0
3	32.424929000241725	34.0	31.0	34.0	30.0	34.0
4	35.9365422758996	37.0	35.0	37.0	35.0	37.0
5	36.01931370027671	37.0	35.0	37.0	35.0	37.0
6	36.01483682472966	37.0	35.0	37.0	35.0	37.0
7	36.02772219088753	37.0	35.0	37.0	35.0	37.0
8	35.92678167174215	37.0	35.0	37.0	35.0	37.0
9	37.739423988591476	39.0	38.0	39.0	35.0	39.0
10	36.999269306801736	39.0	37.0	39.0	32.0	39.0
11	37.03480174704108	39.0	37.0	39.0	33.0	39.0
12	37.16341258006444	39.0	37.0	39.0	34.0	39.0
13	37.23172135644031	39.0	37.0	39.0	34.0	39.0
14	38.42958578967294	40.0	38.0	41.0	34.0	41.0
15	38.449639013277206	40.0	38.0	41.0	34.0	41.0
16	38.57143850532402	40.0	38.0	41.0	34.0	41.0
17	38.60047638547488	40.0	38.0	41.0	34.0	41.0
18	38.360513207113996	39.0	38.0	41.0	34.0	41.0
19	38.05346864034773	39.0	37.0	41.0	34.0	41.0
20	37.55495486148286	39.0	35.0	41.0	34.0	41.0
21	37.47067900382896	39.0	35.0	40.0	34.0	41.0
22	37.47451569500292	39.0	35.0	40.0	34.0	41.0
23	37.34505165073748	39.0	35.0	40.0	33.0	41.0
24	37.27392164805533	39.0	35.0	40.0	33.0	41.0
25	37.210694611082474	39.0	35.0	40.0	33.0	41.0
26	37.20856323862652	39.0	35.0	40.0	33.0	41.0
27	37.15724914982412	39.0	35.0	40.0	33.0	41.0
28	37.11453671071062	39.0	35.0	40.0	33.0	41.0
29	36.95298728792513	39.0	35.0	40.0	33.0	41.0
30	36.68429086887368	39.0	35.0	40.0	32.0	41.0
31	36.32309222297401	39.0	35.0	40.0	31.0	41.0
32	35.836743050964195	39.0	35.0	40.0	28.0	41.0
33	35.083584900037415	39.0	35.0	41.0	21.0	41.0
34	34.528619000892945	39.0	35.0	41.0	16.0	41.0
35	34.15846881143148	39.0	34.0	41.0	14.0	41.0
36	33.885620024084176	39.0	34.0	41.0	10.0	41.0
37	33.74618014125999	39.0	34.0	41.0	10.0	41.0
38	33.66063053745685	39.0	33.0	41.0	10.0	41.0
39	33.6024466080714	39.0	33.0	41.0	10.0	41.0
40	33.502274310173746	39.0	33.0	40.0	10.0	41.0
41	33.35159367277086	38.0	33.0	40.0	10.0	41.0
42	33.07388169172032	38.0	33.0	40.0	10.0	41.0
43	32.98066974323088	38.0	33.0	40.0	10.0	41.0
44	32.965033791801005	38.0	32.0	40.0	10.0	41.0
45	32.978861774259954	38.0	32.0	40.0	9.0	41.0
46	32.749904248285574	38.0	31.0	40.0	8.0	41.0
47	32.652936183551894	38.0	31.0	40.0	8.0	41.0
48	32.5083836558722	38.0	31.0	40.0	8.0	41.0
49	32.507239050363744	38.0	31.0	40.0	8.0	41.0
50	32.35378023353484	38.0	31.0	40.0	8.0	41.0
51	31.44101308073277	36.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	6.0
13	10.0
14	10.0
15	15.0
16	33.0
17	94.0
18	281.0
19	567.0
20	1089.0
21	1758.0
22	3065.0
23	5579.0
24	11124.0
25	22520.0
26	34189.0
27	32530.0
28	25423.0
29	19789.0
30	18170.0
31	19317.0
32	22264.0
33	28534.0
34	41098.0
35	53161.0
36	71211.0
37	101950.0
38	189235.0
39	202903.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.015916308034644	4.317602090091602	45.39845406511558	30.268027536758176
2	35.96268828870991	4.215172590395689	43.650309220089866	16.171829900804536
3	16.590709158720472	4.110756311610848	61.3793324201508	17.919202109517887
4	13.747186775998385	4.36694043746668	62.09953983988768	19.786332946647256
5	12.587349294527858	5.349733826790391	63.0186459217496	19.04427095693215
6	16.09831907451415	6.36144588951963	64.91050112087453	12.629733915091684
7	66.9604156341854	1.6703293307093132	27.22052916757268	4.148725867532608
8	67.7563414125337	1.981370634742806	25.38463491278592	4.877653039937571
9	61.77448070561563	4.985380617203962	28.052879229218014	5.187259447962393
10	27.25894022995864	19.13654580795131	37.971873830697724	15.632640131392323
11	18.896035161574808	20.68457784807542	40.1949692545936	20.224417735756173
12	14.882851778553603	17.41422909108168	47.46282791512921	20.240091215235505
13	18.465014475893195	18.45508058044855	48.79827459273788	14.281630350920373
14	17.700104526655398	20.08534319953112	46.60972704966617	15.604825224147312
15	14.26639837790525	20.232475228727942	47.88711562723167	17.61401076613513
16	13.596743448320012	19.809180906169942	46.27462364333342	20.31945200217663
17	13.842772925499094	20.388437387208896	44.27504086694209	21.493748820349918
18	14.999740614952279	18.666120670339264	47.2691169539586	19.065021760749854
19	15.199191160157573	22.077530742646985	46.1751743122709	16.548103784924542
20	15.41784723655585	23.15944233318506	44.44656612828632	16.976144301972763
21	14.580199097340035	24.455484558863297	44.54104851162652	16.423267832170147
22	13.857894521898167	22.05755257514164	42.861447545168865	21.22310535779132
23	12.736247349581506	23.3585617485422	41.52721501033677	22.377975891539524
24	13.351486607453293	22.33117620633363	42.80647999037516	21.510857195837918
25	12.779294229841643	24.22126537960174	41.21981613463298	21.77962425592364
26	12.907662234309688	24.77943992697483	43.351519720438105	18.961378118277374
27	14.224896770269838	23.692340635482328	43.21586685931065	18.866895734937177
28	12.830508979689597	21.654126043472935	43.705718281347785	21.809646695489683
29	14.125116309359164	19.98710801124517	43.183526510807525	22.704249168588138
30	17.613017376590665	21.354895037356965	40.572236528258074	20.4598510577943
31	16.203839119459506	24.646436104632617	36.83808523061538	22.31163954529249
32	14.932631632392887	23.827993496609782	37.759067714950184	23.480307156047147
33	17.278465853338176	20.702679613107886	36.43002288107251	25.58883165248143
34	17.524053824053052	19.571098545346576	36.559494651701065	26.3453529788993
35	17.77935493698047	19.063034981660927	37.98776806340916	25.169842017949446
36	18.211700142054703	20.519785560310336	36.64845820423868	24.62005609339628
37	18.545368652378784	21.80423824130315	34.37679706928009	25.273596037037976
38	19.055639748385467	21.402356982259167	36.25430330831831	23.287699961037053
39	20.688220276405122	19.61944350317719	39.29495832730861	20.397377893109077
40	25.19853993812287	19.080143357148927	36.4056296489251	19.315687055803103
41	24.927454969100065	20.011280490160477	33.291022297180206	21.77024224355925
42	22.350602490758718	19.910837769553495	36.288188929446164	21.450370810241626
43	18.59205796096862	19.361824481312688	39.31670252067078	22.72941503704791
44	18.412033700188413	19.71083534126794	36.257283476951706	25.61984748159194
45	21.916160129979502	18.385322559103916	35.51235169521926	24.186165615697323
46	24.200073069319828	19.50299617324272	35.359369705371705	20.937561052065753
47	20.866478511328506	21.720020883255756	36.66269678770934	20.750803817706394
48	19.694389225476247	20.690096678878	38.45179135729021	21.163722738355542
49	22.22532503154012	17.700877162967764	38.62662791711599	21.447169888376127
50	21.595405683733468	16.98331878201612	35.83056747929611	25.59070805495431
51	20.420005099399663	16.928351227222404	33.34190591718001	29.309737756197922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302.0
1	803.0
2	1304.0
3	18543.5
4	35783.0
5	24614.0
6	13445.0
7	13321.5
8	13198.0
9	13533.5
10	13869.0
11	14195.5
12	14522.0
13	14411.0
14	14300.0
15	13816.5
16	13333.0
17	12803.5
18	12274.0
19	11577.5
20	10881.0
21	10572.5
22	10264.0
23	9711.0
24	9158.0
25	8956.5
26	8721.5
27	8688.0
28	9098.0
29	9508.0
30	10071.5
31	10635.0
32	11437.5
33	12240.0
34	14313.5
35	16387.0
36	18133.5
37	19880.0
38	22869.5
39	25859.0
40	30089.0
41	34319.0
42	41298.5
43	48278.0
44	58309.0
45	68340.0
46	83108.0
47	97876.0
48	108908.5
49	119941.0
50	112391.5
51	104842.0
52	84133.5
53	63425.0
54	49917.5
55	36410.0
56	28794.0
57	21178.0
58	17608.0
59	14038.0
60	11642.5
61	9247.0
62	7338.5
63	5430.0
64	4512.5
65	3595.0
66	2881.0
67	2167.0
68	1635.5
69	1104.0
70	803.0
71	502.0
72	397.5
73	293.0
74	237.0
75	161.0
76	141.0
77	93.0
78	45.0
79	36.5
80	28.0
81	22.5
82	17.0
83	9.0
84	1.0
85	0.5
86	0.0
87	2.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	905989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.484482673381905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28975936185302	34.70000237850678
2	9.55163756205091	8.689025863870569
3	4.2415062231379155	5.7876814894607405
4	2.440204058583184	4.439656768885722
5	1.629129586765346	3.7050058230961103
6	1.148502958147888	3.1343437740123283
7	0.8712108937234919	2.7738603760299414
8	0.6488113197331086	2.3608677784555696
9	0.5209930751402432	2.132739044925151
>10	2.4746006780465093	18.50574839080429
>50	0.11706495982679739	3.6060980932531175
>100	0.0624333578265935	5.329632013943321
>500	0.0021949227343729264	0.6775463582163691
>1k	0.0017071621267344982	1.7197120483674475
>5k	0.0	0.0
>10k+	2.4388030381921407E-4	2.4380797981724913
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21979	2.4259676441987708	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	3933	0.4341112309310599	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	2737	0.3021007981333107	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	2355	0.2599369308015881	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	2181	0.2407313996086045	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1829	0.20187883075843083	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1436	0.15850082065014034	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	1032	0.11390866776528191	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	930	0.10265025292801569	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	925	0.10209836984775753	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.415064642065191E-4	0.0	0.0	0.3204233163978812	0.0
2	4.415064642065191E-4	0.0	0.0	1.0000121414277656	0.0
3	4.415064642065191E-4	0.0	0.0	1.5113870035949664	0.0
4	4.415064642065191E-4	0.0	0.0	2.24781978589144	0.0
5	4.415064642065191E-4	0.0	0.0	3.856227834995789	0.0
6	4.415064642065191E-4	0.0	0.0	5.566513500715792	0.0
7	4.415064642065191E-4	0.0	0.0	7.11862947563381	0.0
8	4.415064642065191E-4	0.0	0.0	9.62108811475636	0.0
9	4.415064642065191E-4	0.0	0.0	10.906975691757847	0.0
10	4.415064642065191E-4	0.0	0.0	13.37378268389572	0.0
11	4.415064642065191E-4	0.0	0.0	18.29393072101317	0.0
12	4.415064642065191E-4	0.0	0.0	22.374554216441922	0.0
13	4.415064642065191E-4	0.0	0.0	23.857905559559775	0.0
14	4.415064642065191E-4	0.0	0.0	24.395108549883055	0.0
15	4.415064642065191E-4	0.0	0.0	25.0517390387742	0.0
16	4.415064642065191E-4	0.0	0.0	26.795799949006003	0.0
17	4.415064642065191E-4	0.0	0.0	29.25951639589443	0.0
18	4.415064642065191E-4	0.0	0.0	31.652260678661662	0.0
19	4.415064642065191E-4	0.0	0.0	33.05238805327659	0.0
20	4.415064642065191E-4	0.0	0.0	34.26344028459507	0.0
21	4.415064642065191E-4	0.0	0.0	35.67261854172622	0.0
22	4.415064642065191E-4	0.0	0.0	36.86170582645043	0.0
23	4.415064642065191E-4	0.0	0.0	37.84085678744444	0.0
24	4.415064642065191E-4	0.0	0.0	38.43887729321217	0.0
25	4.415064642065191E-4	0.0	0.0	38.8573150446639	0.0
26	4.415064642065191E-4	0.0	0.0	39.18138078939148	0.0
27	4.415064642065191E-4	0.0	0.0	39.483923093989	0.0
28	5.518830802581488E-4	0.0	0.0	39.75887124457361	0.0
29	5.518830802581488E-4	0.0	0.0	40.03260525238165	0.0
30	6.622596963097786E-4	0.0	0.0	40.313734493465155	0.0
31	7.726363123614084E-4	0.0	0.0	40.561640373117115	0.0
32	8.830129284130381E-4	0.0	0.0	40.802261396109664	0.0
33	8.830129284130381E-4	0.0	0.0	41.041447523093545	0.0
34	8.830129284130381E-4	0.0	0.0	41.26628468999072	0.0
35	9.93389544464668E-4	0.0	0.0	41.50447742743013	0.0
36	9.93389544464668E-4	0.0	0.0	41.73295702265701	0.0
37	9.93389544464668E-4	0.0	0.0	41.992121317146236	0.0
38	9.93389544464668E-4	0.0	0.0	42.2334045998351	0.0
39	0.0011037661605162977	0.0	0.0	42.49841885497506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	25	3.890149E-5	45.000004	1
CGTTAGG	50	2.1827873E-11	45.000004	2
CTACGAA	25	3.890149E-5	45.000004	11
CCGGCGG	20	7.032494E-4	45.0	2
TACGAAT	20	7.032494E-4	45.0	12
TCGCTAG	20	7.032494E-4	45.0	1
CGTTTTT	17590	0.0	44.53951	1
TTGCGCG	85	0.0	42.352943	1
GTCTTCG	55	6.002665E-11	40.909092	1
CTGTGCG	55	6.002665E-11	40.909092	1
TGTACGG	50	1.0804797E-9	40.500004	2
CGTCTGG	95	0.0	40.263157	2
ATGGGCG	45	1.927583E-8	40.0	5
GTTTTTT	20185	0.0	39.604904	2
TATCGGG	40	3.457135E-7	39.375	3
TACGGGA	120	0.0	39.374996	4
GCGGGCA	235	0.0	38.29787	5
GGACCGA	290	0.0	38.01724	8
TAGCGGG	95	0.0	37.894737	3
GACCGAT	285	0.0	37.894737	9
>>END_MODULE