##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933702.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1291545 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38043428606824 33.0 31.0 34.0 31.0 34.0 2 32.6089481977012 34.0 31.0 34.0 31.0 34.0 3 32.62516133777762 34.0 31.0 34.0 31.0 34.0 4 36.15209226159367 37.0 35.0 37.0 35.0 37.0 5 36.173170892225976 37.0 35.0 37.0 35.0 37.0 6 36.15734023979033 37.0 36.0 37.0 35.0 37.0 7 36.24940594404376 37.0 37.0 37.0 35.0 37.0 8 36.15565311313195 37.0 37.0 37.0 35.0 37.0 9 37.97645533063114 39.0 39.0 39.0 35.0 39.0 10 37.3921698430949 39.0 37.0 39.0 34.0 39.0 11 37.31898849827145 39.0 37.0 39.0 34.0 39.0 12 37.42157183837961 39.0 37.0 39.0 34.0 39.0 13 37.44684389626378 39.0 37.0 39.0 35.0 39.0 14 38.746223321680624 40.0 38.0 41.0 35.0 41.0 15 38.77001420778989 40.0 38.0 41.0 35.0 41.0 16 38.8025016549946 40.0 38.0 41.0 35.0 41.0 17 38.75669527581308 40.0 38.0 41.0 34.0 41.0 18 38.21772605677696 39.0 38.0 40.0 34.0 41.0 19 37.656233425858176 38.0 37.0 40.0 34.0 41.0 20 37.004853102292216 38.0 35.0 40.0 34.0 41.0 21 36.89816692411027 38.0 35.0 40.0 33.0 41.0 22 36.90910963226214 38.0 35.0 40.0 33.0 41.0 23 36.836656872195704 38.0 35.0 40.0 33.0 41.0 24 36.71943989562888 37.0 35.0 40.0 33.0 41.0 25 36.6570216291341 37.0 35.0 40.0 33.0 41.0 26 36.62563596312943 37.0 35.0 40.0 33.0 41.0 27 36.549537182212 37.0 35.0 40.0 33.0 41.0 28 36.39816808550999 37.0 35.0 40.0 33.0 41.0 29 36.24314754809163 37.0 35.0 40.0 32.0 41.0 30 36.00835510957806 37.0 35.0 40.0 31.0 41.0 31 35.49870349078042 37.0 35.0 40.0 30.0 41.0 32 34.66207913777685 37.0 35.0 40.0 22.0 41.0 33 33.595149220507224 37.0 33.0 40.0 16.0 41.0 34 32.59873794563875 37.0 32.0 40.0 10.0 41.0 35 31.955830420155706 37.0 30.0 40.0 8.0 41.0 36 31.60185436821791 37.0 30.0 40.0 7.0 41.0 37 31.28906387311321 37.0 27.0 40.0 7.0 41.0 38 31.143642691505136 36.0 26.0 40.0 7.0 41.0 39 31.10598082141931 37.0 25.0 40.0 7.0 41.0 40 31.003241853748804 36.0 24.0 40.0 7.0 41.0 41 30.865393772574706 36.0 23.0 40.0 7.0 41.0 42 30.717801547758693 36.0 23.0 40.0 7.0 41.0 43 30.53295394275848 36.0 23.0 40.0 7.0 41.0 44 30.361579348764465 36.0 22.0 40.0 7.0 41.0 45 30.339641282340143 36.0 23.0 40.0 7.0 41.0 46 30.164648541088386 35.0 22.0 40.0 7.0 41.0 47 30.01162793398604 35.0 22.0 40.0 7.0 41.0 48 29.9197457308882 35.0 22.0 40.0 7.0 41.0 49 29.901061132209872 35.0 22.0 40.0 7.0 41.0 50 29.806168581040538 35.0 20.0 40.0 7.0 41.0 51 28.960098176989575 35.0 20.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 5.0 11 9.0 12 4.0 13 7.0 14 13.0 15 37.0 16 78.0 17 209.0 18 568.0 19 1110.0 20 2060.0 21 3289.0 22 5397.0 23 9331.0 24 18780.0 25 39287.0 26 64934.0 27 69781.0 28 56807.0 29 44615.0 30 38048.0 31 36111.0 32 37252.0 33 43384.0 34 58476.0 35 80485.0 36 104583.0 37 131344.0 38 223456.0 39 222028.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.692755575686485 3.566658536868634 41.05470579809453 39.68588008935035 2 46.131958236066104 3.2379824164082556 37.44948879055705 13.180570556968593 3 14.165824651870434 3.3349205796158863 68.20606328079936 14.293191487714326 4 11.944376696127506 3.4733594261136855 68.56238071457054 16.019883163188275 5 11.604396285069432 4.369960009136344 68.1535680135032 15.872075692291016 6 14.328188332578423 5.125334386335745 69.32859482248006 11.217882458605779 7 58.23583382692822 1.1311258996008657 37.39134137796205 3.2416988955088675 8 59.97475891277501 1.4141977244308175 35.52690769582167 3.084135666972502 9 55.28309118149194 4.029360184894835 37.16951403164427 3.5180346019689597 10 23.485205703246887 22.006666434386723 42.95831736408719 11.549810498279193 11 16.611267900073166 18.878242724798593 50.05787641932724 14.452612955800998 12 14.441850651738807 17.445462604864716 52.8363316802744 15.276355063122073 13 15.638711775431752 17.291383575485174 54.35157118025311 12.718333468829968 14 14.793677339930083 18.584098889314735 53.47680491194654 13.145418858808636 15 12.760298712007712 19.16301793588299 54.1275758877933 13.949107464315993 16 13.682759795438795 19.01157141253305 52.74194859644844 14.563720195579712 17 13.899476982993237 18.922143634174574 51.66842812290706 15.509951259925128 18 14.656787026390871 17.8881107510772 52.65244339144203 14.802658831089897 19 14.576882725727714 20.267431641948207 51.68120351981541 13.474482112508662 20 15.206206520098023 20.987344614395937 50.030389959312295 13.776058906193745 21 14.720276877692996 22.050335063818917 50.29240173590544 12.936986322582644 22 14.00950024970094 20.1358063404682 50.11943060443112 15.735262805399735 23 12.68356890390966 21.50803882172127 49.39804652567274 16.41034574869633 24 12.344827319218457 21.5931307077957 49.93771026174078 16.12433171124506 25 12.747910448339006 22.554692248431145 48.886798369394796 15.810598933835058 26 13.43631077507946 22.83559612711907 48.99519567649598 14.732897421305491 27 13.584273099272576 22.06961429915334 49.81289850527856 14.533214096295522 28 13.152929243657791 21.197557963524304 49.80492356054183 15.844589232276071 29 14.547305746218676 20.277884239418682 48.79829971081147 16.37651030355117 30 16.888532726308412 20.84456987561409 47.23195862319935 15.034938774878151 31 16.36985161182924 22.853791389382483 45.57858998331456 15.197767015473715 32 15.860151988509886 23.102253502587985 44.75693839548758 16.280656113414555 33 17.15209303586015 23.258190771517835 42.13960798888154 17.45010820374048 34 16.2849920056986 24.090759516702864 40.89265182397826 18.73159665362028 35 17.823691779999923 25.389978668958495 38.082916197267615 18.703413353773968 36 18.93189939181368 25.926545339109364 37.239817427964184 17.901737841112777 37 17.247250386165405 27.702944922553996 38.05457804412545 16.99522664715515 38 15.78884204576689 28.230994661432625 38.01276765424356 17.967395638556923 39 16.33005431479352 27.264477815329702 35.91001474977643 20.495453120100347 40 17.474110464598603 27.442559105567366 36.10141342345795 18.981917006376083 41 17.135988293090833 27.19154191298019 36.46407984235934 19.208389951569632 42 18.098633806797285 26.440890561304485 36.69194646721562 18.768529164682608 43 17.356809092985532 26.169277880368085 37.595980008439504 18.87793301820688 44 17.396761243317112 26.802937567022443 35.86595898710459 19.934342202555854 45 18.26804331246685 26.23756818384183 35.34983295200709 20.144555551684224 46 18.495058244195906 26.49315354865684 36.03583305266174 18.97595515448552 47 16.4913340224305 27.694273137985903 37.00041423256642 18.813978607017177 48 16.18913781556198 26.57050276993833 37.85017169359178 19.390187720907907 49 17.712894246812926 25.358466023251225 36.96526253440647 19.963377195529386 50 16.727640151911082 24.972649036618932 36.56488933796345 21.734821473506535 51 16.0312648804339 24.741220785957903 35.52504945627136 23.702464877336833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 460.0 1 1451.5 2 2443.0 3 56734.0 4 111025.0 5 72855.5 6 34686.0 7 33917.0 8 33148.0 9 33105.5 10 33063.0 11 32817.0 12 32571.0 13 31769.0 14 30967.0 15 29680.5 16 28394.0 17 27241.5 18 26089.0 19 24500.5 20 22912.0 21 21847.0 22 20782.0 23 20172.5 24 19563.0 25 19631.5 26 19314.0 27 18928.0 28 19764.0 29 20600.0 30 21650.0 31 22700.0 32 24674.5 33 26649.0 34 30061.5 35 33474.0 36 35798.0 37 38122.0 38 41494.5 39 44867.0 40 47860.0 41 50853.0 42 56900.0 43 62947.0 44 69379.5 45 75812.0 46 85920.5 47 96029.0 48 101668.5 49 107308.0 50 100489.0 51 93670.0 52 78471.0 53 63272.0 54 51936.5 55 40601.0 56 33843.5 57 27086.0 58 23146.5 59 19207.0 60 16064.5 61 12922.0 62 10691.0 63 8460.0 64 6822.0 65 5184.0 66 3949.0 67 2714.0 68 2152.0 69 1590.0 70 1278.5 71 967.0 72 845.0 73 723.0 74 570.5 75 393.5 76 369.0 77 253.5 78 138.0 79 110.5 80 83.0 81 56.5 82 30.0 83 17.0 84 4.0 85 4.0 86 4.0 87 6.0 88 8.0 89 4.5 90 1.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1291545.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.63737367814606 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62098250132607 35.880551573560005 2 8.861307613140422 8.088136136357798 3 3.7478770121978986 5.131297911162272 4 2.0706968404552795 3.7800466192805597 5 1.32302129606106 3.018960863624185 6 0.9119089576346381 2.4970277916012384 7 0.7385263157785684 2.3593081011031716 8 0.5500953506482548 2.008392566091615 9 0.4608746446624685 1.8929797539519475 >10 2.5651028786761754 19.732523759437964 >50 0.08927801006729098 2.815282138421125 >100 0.05506045307248146 4.370202066111536 >500 0.002888972475817499 0.9278793958269719 >1k 0.002209214246213382 1.7658714446325916 >5k 0.0 0.0 >10k+ 1.6993955740102936E-4 5.731539878836918 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73902 5.72198413527984 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC 4976 0.38527500009678334 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC 2685 0.20789054969048698 No Hit CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT 2243 0.1736679713056843 TruSeq Adapter, Index 11 (96% over 27bp) CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC 1695 0.13123816824036327 RNA PCR Primer, Index 11 (96% over 26bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1460 0.11304290597695009 No Hit TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC 1371 0.1061519343112319 Illumina PCR Primer Index 11 (100% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5485329585883574E-4 0.0 0.0 0.18791447452469717 0.0 2 1.5485329585883574E-4 0.0 0.0 0.5504260401302317 0.0 3 1.5485329585883574E-4 0.0 0.0 0.8551773263804203 0.0 4 1.5485329585883574E-4 0.0 0.0 1.247188444847063 0.0 5 1.5485329585883574E-4 0.0 0.0 2.049483370691691 0.0 6 1.5485329585883574E-4 0.0 0.0 3.1661304871297555 0.0 7 1.5485329585883574E-4 0.0 0.0 4.255136290257018 0.0 8 1.5485329585883574E-4 0.0 0.0 5.822174217700506 0.0 9 1.5485329585883574E-4 0.0 0.0 6.79922108792183 0.0 10 2.322799437882536E-4 0.0 0.0 8.16402061097368 0.0 11 2.322799437882536E-4 0.0 0.0 10.71491895365628 0.0 12 2.322799437882536E-4 0.0 0.0 12.813877952374868 0.0 13 2.322799437882536E-4 0.0 0.0 13.685856861355973 0.0 14 2.322799437882536E-4 0.0 0.0 14.059595290911274 0.0 15 2.322799437882536E-4 0.0 0.0 14.430236654549397 0.0 16 2.322799437882536E-4 0.0 0.0 15.22502119554487 0.0 17 2.322799437882536E-4 0.0 0.0 16.26501593053281 0.0 18 3.097065917176715E-4 0.0 0.0 17.37182986268384 0.0 19 3.097065917176715E-4 0.0 0.0 18.043196326879823 0.0 20 3.097065917176715E-4 0.0 0.0 18.655099125466013 0.0 21 3.097065917176715E-4 0.0 0.0 19.34822247773016 0.0 22 3.097065917176715E-4 0.0 0.0 19.986837469852 0.0 23 3.097065917176715E-4 0.0 0.0 20.51852626118331 0.0 24 3.8713323964708934E-4 0.0 0.0 20.881115253436775 0.0 25 3.8713323964708934E-4 0.0 0.0 21.172936289482752 0.0 26 3.8713323964708934E-4 0.0 0.0 21.42883136088948 0.0 27 3.8713323964708934E-4 0.0 0.0 21.68883004463646 0.0 28 5.41986535505925E-4 0.0 0.0 21.9379115710254 0.0 29 6.19413183435343E-4 0.0 0.0 22.20503350638189 0.0 30 6.19413183435343E-4 0.0 0.0 22.48175634608163 0.0 31 6.19413183435343E-4 0.0 0.0 22.73827083067179 0.0 32 6.19413183435343E-4 0.0 0.0 23.01530337696325 0.0 33 6.19413183435343E-4 0.0 0.0 23.255403412192376 0.0 34 6.19413183435343E-4 0.0 0.0 23.48745107603684 0.0 35 6.19413183435343E-4 0.0 0.0 23.742804160908058 0.0 36 6.968398313647608E-4 0.0 0.0 23.995369886453823 0.0 37 6.968398313647608E-4 0.0 0.0 24.261640128683087 0.0 38 6.968398313647608E-4 0.0 0.0 24.520477412711134 0.0 39 6.968398313647608E-4 0.0 0.0 24.8375395359821 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCCG 30 2.1657106E-6 45.000004 1 GTCGAGA 35 1.2120836E-7 45.0 35 CAACGGT 20 7.0336106E-4 45.0 7 CCGTGCG 20 7.0336106E-4 45.0 1 ATTCCGG 45 3.8562575E-10 45.0 2 TCGCGCT 20 7.0336106E-4 45.0 3 CGTTTTT 40225 0.0 44.60286 1 GTTTTTT 44370 0.0 40.786003 2 CTTCGCG 40 3.4586628E-7 39.375 1 TGGGTCG 110 0.0 38.863632 6 ACGGGAC 105 0.0 38.57143 5 GCGTTAG 35 6.248889E-6 38.571426 1 TATTCCG 35 6.248889E-6 38.571426 1 TATCCGG 35 6.248889E-6 38.571426 2 CTAGCGG 35 6.248889E-6 38.571426 2 TGGTTCG 95 0.0 37.894737 1 CCGGGAT 215 0.0 37.67442 5 GCCGCCC 30 1.14E-4 37.500004 16 AGGCACG 120 0.0 37.500004 10 TCGAGAT 30 1.14E-4 37.500004 36 >>END_MODULE