##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297579
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.303352628240745	33.0	31.0	34.0	30.0	34.0
2	32.54738015951244	34.0	31.0	34.0	31.0	34.0
3	32.578803294442956	34.0	31.0	34.0	31.0	34.0
4	36.07590289300305	37.0	35.0	37.0	35.0	37.0
5	36.14908918840394	37.0	35.0	37.0	35.0	37.0
6	36.15320377410547	37.0	36.0	37.0	35.0	37.0
7	36.17406416102603	37.0	37.0	37.0	35.0	37.0
8	36.115509730043414	37.0	37.0	37.0	35.0	37.0
9	37.94386391888278	39.0	39.0	39.0	35.0	39.0
10	37.41061854422737	39.0	37.0	39.0	34.0	39.0
11	37.32743439898457	39.0	37.0	39.0	34.0	39.0
12	37.40114243525828	39.0	37.0	39.0	34.0	39.0
13	37.44054042181632	39.0	37.0	39.0	35.0	39.0
14	38.768983622577124	40.0	38.0	41.0	35.0	41.0
15	38.77628414146653	40.0	38.0	41.0	35.0	41.0
16	38.86398053605985	40.0	38.0	41.0	35.0	41.0
17	38.84766707845919	40.0	38.0	41.0	35.0	41.0
18	38.364104998616654	39.0	38.0	40.0	35.0	41.0
19	37.8372769596302	38.0	37.0	40.0	35.0	41.0
20	37.146639241233096	38.0	35.0	40.0	34.0	41.0
21	37.077362534381336	38.0	35.0	40.0	33.0	41.0
22	37.060381680036436	38.0	35.0	40.0	33.0	41.0
23	36.93252896355443	38.0	35.0	40.0	33.0	41.0
24	36.82098739267513	38.0	35.0	40.0	33.0	41.0
25	36.76938128622612	38.0	35.0	40.0	33.0	41.0
26	36.77048025592276	38.0	35.0	40.0	33.0	41.0
27	36.71018257847884	38.0	35.0	40.0	33.0	41.0
28	36.615191059658024	38.0	35.0	40.0	33.0	41.0
29	36.4036447877162	37.0	35.0	40.0	33.0	41.0
30	36.07225533088929	37.0	35.0	40.0	31.0	41.0
31	35.556219698376744	37.0	35.0	40.0	30.0	41.0
32	34.80591856064255	37.0	35.0	40.0	23.0	41.0
33	33.6763711496564	37.0	33.0	40.0	16.0	41.0
34	32.70120431973699	37.0	32.0	40.0	10.0	41.0
35	32.11507199176312	37.0	31.0	40.0	8.0	41.0
36	31.739180427550075	37.0	30.0	40.0	7.0	41.0
37	31.47477340493334	37.0	28.0	40.0	7.0	41.0
38	31.303979179687712	37.0	27.0	40.0	7.0	41.0
39	31.308873679367498	37.0	27.0	40.0	7.0	41.0
40	31.211294264164263	37.0	25.0	40.0	7.0	41.0
41	31.097627196494393	37.0	24.0	40.0	7.0	41.0
42	30.876569364948107	37.0	23.0	40.0	7.0	41.0
43	30.718303856643796	36.0	23.0	40.0	7.0	41.0
44	30.55622971703457	36.0	23.0	40.0	7.0	41.0
45	30.504609738597804	36.0	23.0	40.0	7.0	41.0
46	30.309659758673654	35.0	23.0	40.0	7.0	41.0
47	30.253252403129213	35.0	23.0	40.0	7.0	41.0
48	30.12240333729199	35.0	22.0	40.0	7.0	41.0
49	30.078282709569127	35.0	22.0	40.0	7.0	41.0
50	29.905041619816597	35.0	21.0	40.0	7.0	41.0
51	29.175627071646506	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	3.0
11	6.0
12	8.0
13	13.0
14	24.0
15	24.0
16	78.0
17	209.0
18	518.0
19	1085.0
20	2050.0
21	3167.0
22	5324.0
23	9250.0
24	18108.0
25	38792.0
26	64537.0
27	69090.0
28	57301.0
29	44767.0
30	37722.0
31	35148.0
32	36349.0
33	42084.0
34	57592.0
35	77570.0
36	100757.0
37	124305.0
38	222623.0
39	248984.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.827783896009414	3.714455921373573	40.9852502236858	39.47250995893121
2	46.03257296858226	3.2826517691793717	37.447739212795526	13.237036049442846
3	14.349646534045327	3.4153604520418406	67.88365101469736	14.35134199921546
4	12.218292681986993	3.554080329598429	68.13650652484358	16.091120463571006
5	11.622799074276017	4.477184048138881	67.91440058755575	15.985616290029355
6	14.412378745340359	5.11938001462724	69.16472908393246	11.303512156099938
7	58.35136049519913	1.2010829398441252	37.11712350461899	3.3304330603377523
8	60.137918384930714	1.4309726035948485	35.320392823866605	3.110716187607845
9	55.43300253780309	4.070272407306222	36.88399704372527	3.6127280111654088
10	23.600875168294184	22.134528995922405	42.668770071032284	11.595825764751124
11	16.66149036012451	18.997918431170664	49.86285998771559	14.477731220989243
12	14.565510076843106	17.575114887031926	52.60720156537675	15.252173470748218
13	15.717809859746499	17.449110998251356	54.02946564332499	12.803613498677151
14	14.827690645425056	18.785060485719946	53.217723159823024	13.169525709031973
15	12.840682532624218	19.265570728256236	53.90662148508877	13.987125254030774
16	13.785210765587298	19.151358029067982	52.45491796645908	14.608513238885648
17	14.006160703895484	18.9820427118503	51.454978849072	15.556817735182213
18	14.777366156511473	17.99358651766097	52.38810122543599	14.840946100391575
19	14.6198420288861	20.365156957688125	51.42877620553353	13.586224807892236
20	15.262731594762247	21.1680367823462	49.761671543697915	13.807560079193637
21	14.845878362704697	22.059157862449993	50.070015004866754	13.024948769978554
22	14.098024089477404	20.201236302375424	50.01822625057897	15.68251335756821
23	12.87436063623101	21.5791870861042	49.08625987319462	16.46019240447017
24	12.480858583562156	21.73193308461373	49.75388781723502	16.033320514589093
25	12.880988363714271	22.68193304608043	48.6339560057615	15.803122584443798
26	13.470470776731128	22.914211774389074	48.74724390576605	14.868073543113752
27	13.654659947486818	22.160269239869017	49.57324371001688	14.611827102627279
28	13.2313331211433	21.331417971468404	49.55813865668294	15.879110250705352
29	14.670628917391543	20.365234024286767	48.54594595011171	16.41819110820998
30	17.045667354357615	20.984232944583724	46.961533748619544	15.008565952439119
31	16.447861748687362	22.891862460782736	45.43969962522513	15.220576165304772
32	15.91957021499269	23.344705794406355	44.48669406641137	16.249029924189585
33	17.23995224953548	23.313339688758834	41.9522819034525	17.494426158253177
34	16.326327722628065	24.363063828868995	40.544352212851784	18.766256235651163
35	17.834752257858675	25.53879185775972	37.9573806296187	18.66907525476291
36	19.004854425048496	26.11324628404128	36.98919295087236	17.89270634003787
37	17.27409275273413	27.675771571518958	37.98396860615038	17.06616706959653
38	15.8667025283239	28.241132139160698	37.90643960791597	17.985725724599426
39	16.435608159503197	27.380529432119356	35.82648917715222	20.35737323122523
40	17.535194388935086	27.516860245118025	35.987789568111076	18.960155797835814
41	17.235328253616927	27.330744409396267	36.41026866186953	19.023658675117275
42	18.08745363480759	26.645314081069436	36.50721844296185	18.760013841161115
43	17.337133230423735	26.27161814425172	37.562028978582426	18.829219646742125
44	17.38784305233053	26.9022541209437	35.78880361041602	19.92109921630976
45	18.290061722638853	26.29396745785806	35.34089253910552	20.07507828039757
46	18.494211142442964	26.62157756868753	36.03310472811289	18.85110656075661
47	16.54034166705842	27.71399660444566	37.01539559441082	18.730266134085092
48	16.241169131128046	26.75020172182195	37.730496563215034	19.278132583834974
49	17.686938521662267	25.497638294084602	37.02333345407101	19.79208973018213
50	16.793274243803268	25.012195789235182	36.54698480786141	21.64754515910014
51	16.026769853704476	24.891432429162307	35.528087307208274	23.553710409924943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	467.0
1	1417.5
2	2368.0
3	56317.0
4	110266.0
5	72843.0
6	35420.0
7	34371.0
8	33322.0
9	33357.0
10	33392.0
11	33103.0
12	32814.0
13	31661.0
14	30508.0
15	29388.0
16	28268.0
17	26881.5
18	25495.0
19	24050.5
20	22606.0
21	21650.5
22	20695.0
23	19965.5
24	19236.0
25	19424.5
26	19276.5
27	18940.0
28	19986.5
29	21033.0
30	22022.0
31	23011.0
32	24968.5
33	26926.0
34	30028.0
35	33130.0
36	35884.0
37	38638.0
38	41963.0
39	45288.0
40	48810.0
41	52332.0
42	58045.5
43	63759.0
44	70169.0
45	76579.0
46	86085.5
47	95592.0
48	101951.0
49	108310.0
50	101204.0
51	94098.0
52	78815.5
53	63533.0
54	52141.5
55	40750.0
56	34073.5
57	27397.0
58	23330.0
59	19263.0
60	16324.0
61	13385.0
62	10959.5
63	8534.0
64	6936.0
65	5338.0
66	4023.5
67	2709.0
68	2201.5
69	1694.0
70	1373.0
71	1052.0
72	881.0
73	710.0
74	560.5
75	403.5
76	396.0
77	283.0
78	170.0
79	129.5
80	89.0
81	55.5
82	22.0
83	14.0
84	6.0
85	4.0
86	2.0
87	6.0
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1297579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.450701743104304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22319841604329	35.55299260599252
2	9.058087342740642	8.233928523557863
3	3.7818307508481634	5.156605844991002
4	2.1713156985923865	3.9475128882737085
5	1.3692437901318553	3.111654555944033
6	0.9470739173449821	2.582710448755212
7	0.6977324014888658	2.2198699093599243
8	0.5564403224671852	2.0232482507434253
9	0.4535796429347292	1.8553961760993114
>10	2.5878531997736887	19.747560747706665
>50	0.09320450425798427	2.911592572712383
>100	0.055132253269239824	4.306316759115179
>500	0.0030819252234140677	0.9910353236768958
>1k	0.002054616815609378	1.6847989601574238
>5k	0.0	0.0
>10k+	1.7121806796744818E-4	5.674776432914535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72922	5.619850506211954	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC	4934	0.38024659770233643	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	2807	0.21632594238963485	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	2278	0.17555771170772647	TruSeq Adapter, Index 11 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1703	0.13124441748826082	RNA PCR Primer, Index 11 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1481	0.11413563258961497	No Hit
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1357	0.1045793743579389	Illumina PCR Primer Index 11 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	1333	0.10272977599051773	TruSeq Adapter, Index 11 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5413319728509785E-4	0.0	0.0	0.1872718347013939	0.0
2	1.5413319728509785E-4	0.0	0.0	0.5370771259399235	0.0
3	2.3119979592764678E-4	0.0	0.0	0.8453435205101192	0.0
4	2.3119979592764678E-4	0.0	0.0	1.2442402350839525	0.0
5	2.3119979592764678E-4	0.0	0.0	2.068544574164656	0.0
6	2.3119979592764678E-4	0.0	0.0	3.1746814644811607	0.0
7	2.3119979592764678E-4	0.0	0.0	4.2505311815311435	0.0
8	2.3119979592764678E-4	0.0	0.0	5.788318090844565	0.0
9	2.3119979592764678E-4	0.0	0.0	6.758432434556971	0.0
10	2.3119979592764678E-4	0.0	0.0	8.09415072222963	0.0
11	2.3119979592764678E-4	0.0	0.0	10.618004761174465	0.0
12	2.3119979592764678E-4	0.0	0.0	12.69741572574772	0.0
13	3.082663945701957E-4	0.0	0.0	13.553702703265081	0.0
14	3.082663945701957E-4	0.0	0.0	13.921618645184608	0.0
15	3.082663945701957E-4	0.0	0.0	14.292154851457985	0.0
16	3.8533299321274464E-4	0.0	0.0	15.076769892237776	0.0
17	3.8533299321274464E-4	0.0	0.0	16.128343630715356	0.0
18	3.8533299321274464E-4	0.0	0.0	17.172827242117823	0.0
19	4.6239959185529356E-4	0.0	0.0	17.84037811955958	0.0
20	4.6239959185529356E-4	0.0	0.0	18.43294319652214	0.0
21	4.6239959185529356E-4	0.0	0.0	19.119760723624534	0.0
22	4.6239959185529356E-4	0.0	0.0	19.735676979975786	0.0
23	4.6239959185529356E-4	0.0	0.0	20.273216505507566	0.0
24	4.6239959185529356E-4	0.0	0.0	20.625102594909443	0.0
25	4.6239959185529356E-4	0.0	0.0	20.91125087566923	0.0
26	4.6239959185529356E-4	0.0	0.0	21.166341317176062	0.0
27	6.165327891403914E-4	0.0	0.0	21.422125358070684	0.0
28	6.165327891403914E-4	0.0	0.0	21.671667004475257	0.0
29	6.165327891403914E-4	0.0	0.0	21.93022544292101	0.0
30	6.165327891403914E-4	0.0	0.0	22.20966892959889	0.0
31	6.935993877829403E-4	0.0	0.0	22.461599640561385	0.0
32	6.935993877829403E-4	0.0	0.0	22.72940607084424	0.0
33	7.706659864254893E-4	0.0	0.0	22.969545592214423	0.0
34	7.706659864254893E-4	0.0	0.0	23.196969124808586	0.0
35	8.477325850680382E-4	0.0	0.0	23.446048371621306	0.0
36	8.477325850680382E-4	0.0	0.0	23.68988708972633	0.0
37	8.477325850680382E-4	0.0	0.0	23.955843921641765	0.0
38	9.247991837105871E-4	0.0	0.0	24.2218007535572	0.0
39	9.247991837105871E-4	0.0	0.0	24.533072745474456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	30	2.165718E-6	45.000004	1
CCGTCGG	25	3.891084E-5	45.000004	2
TACTCCG	20	7.033623E-4	45.0	1
CGTTTTT	39630	0.0	44.489025	1
TGTACGG	55	6.184564E-11	40.909092	2
TGTTTCG	145	0.0	40.34483	1
GTTTTTT	44435	0.0	40.083267	2
GCTTACG	40	3.458681E-7	39.375	1
CGGGCGC	35	6.2489107E-6	38.57143	6
CTTACGG	95	0.0	37.894737	2
CGGAATC	30	1.14000286E-4	37.500004	6
TACGGGA	150	0.0	37.5	4
GCGAGAC	115	0.0	37.173916	21
ACGGGAC	110	0.0	36.818184	5
CTGCGCG	55	2.750312E-9	36.818184	1
TTACCGG	55	2.750312E-9	36.818184	2
TTACGGG	185	0.0	36.486485	3
TTGGGCG	1565	0.0	36.08626	5
TTGGGAC	2595	0.0	36.069366	5
TCCGAAG	25	0.002107208	36.000004	13
>>END_MODULE