SRR2933700.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933700.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954581
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28339	2.968737068933909	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3836	0.4018517024746983	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	2469	0.25864751131648334	RNA PCR Primer, Index 29 (96% over 26bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1962	0.20553520340337805	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1771	0.1855264246826618	RNA PCR Primer, Index 29 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1563	0.1637367598977981	Illumina PCR Primer Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1447	0.1515848314600856	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	30	2.165114E-6	44.999996	9
CGTTTTT	12960	0.0	43.90625	1
CGAACGA	50	1.0804797E-9	40.5	22
GATAACG	50	1.0804797E-9	40.5	17
CGCACGG	45	1.927765E-8	40.000004	2
GTTTTTT	15005	0.0	39.496834	2
CGTTCAT	40	3.4574077E-7	39.375	17
GCTACGA	110	0.0	38.863636	10
TTGGGAC	1685	0.0	38.590504	5
TCGTTAG	35	6.247177E-6	38.571426	1
GCGTACG	35	6.247177E-6	38.571426	1
AACACGT	550	0.0	38.045456	41
CGAGACA	730	0.0	37.91096	22
CGTTTGG	375	0.0	37.8	2
TCTGGGA	2120	0.0	37.78302	4
AGGCACG	215	0.0	37.67442	10
ACGACCA	705	0.0	37.659573	28
GCGAGAC	735	0.0	37.65306	21
ACACGAC	720	0.0	37.500004	26
CACGACC	720	0.0	37.500004	27