##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933700.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954581
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05934121881747	33.0	31.0	34.0	30.0	34.0
2	32.24149757851874	34.0	31.0	34.0	30.0	34.0
3	32.38008194171055	34.0	31.0	34.0	30.0	34.0
4	35.932237285259184	37.0	35.0	37.0	35.0	37.0
5	35.94224586493969	37.0	35.0	37.0	35.0	37.0
6	35.90146357407072	37.0	35.0	37.0	35.0	37.0
7	35.96259615475272	37.0	35.0	37.0	35.0	37.0
8	35.78238619876155	37.0	35.0	37.0	35.0	37.0
9	37.57555304369142	39.0	37.0	39.0	35.0	39.0
10	37.07442008588061	39.0	37.0	39.0	33.0	39.0
11	37.09117193826401	39.0	37.0	39.0	33.0	39.0
12	37.043529045727915	39.0	37.0	39.0	33.0	39.0
13	36.960584801080266	39.0	37.0	39.0	33.0	39.0
14	37.905370000031425	40.0	37.0	41.0	33.0	41.0
15	37.985497301957615	40.0	37.0	41.0	33.0	41.0
16	38.10086938667332	40.0	37.0	41.0	33.0	41.0
17	38.05970891941072	40.0	37.0	41.0	33.0	41.0
18	37.87336538229862	39.0	37.0	40.0	33.0	41.0
19	37.61990758248907	39.0	37.0	40.0	33.0	41.0
20	37.29205902903997	39.0	35.0	40.0	33.0	41.0
21	37.13929881277755	39.0	35.0	40.0	33.0	41.0
22	37.2121098157202	39.0	35.0	40.0	33.0	41.0
23	37.12746849141141	39.0	35.0	40.0	33.0	41.0
24	37.05353448266831	39.0	35.0	40.0	33.0	41.0
25	36.969824457013075	38.0	35.0	40.0	32.0	41.0
26	36.91882616561612	38.0	35.0	40.0	32.0	41.0
27	36.85208379383206	38.0	35.0	40.0	32.0	41.0
28	36.70527592734404	38.0	35.0	40.0	32.0	41.0
29	36.62695884372306	38.0	35.0	40.0	32.0	41.0
30	36.48617246729193	38.0	35.0	40.0	31.0	41.0
31	36.23030104307544	38.0	35.0	40.0	31.0	41.0
32	35.90740021014456	38.0	35.0	40.0	30.0	41.0
33	35.54332214867046	38.0	35.0	40.0	27.0	41.0
34	35.20649583429798	38.0	35.0	40.0	24.0	41.0
35	34.91919910410955	38.0	34.0	40.0	23.0	41.0
36	34.7242476018274	38.0	34.0	40.0	22.0	41.0
37	34.61406313345855	38.0	34.0	40.0	21.0	41.0
38	34.58334494401208	38.0	34.0	40.0	21.0	41.0
39	34.48471737861952	38.0	34.0	40.0	20.0	41.0
40	34.30027310411584	38.0	34.0	40.0	19.0	41.0
41	34.187619489598056	38.0	34.0	40.0	18.0	41.0
42	34.13689356901091	38.0	33.0	40.0	18.0	41.0
43	34.045624205803385	38.0	33.0	40.0	18.0	41.0
44	33.941798548263584	38.0	33.0	40.0	18.0	41.0
45	33.88492333285494	37.0	33.0	40.0	18.0	41.0
46	33.64806339116324	37.0	33.0	40.0	18.0	41.0
47	33.503386302471974	37.0	33.0	40.0	18.0	41.0
48	33.459649835896585	37.0	33.0	40.0	18.0	41.0
49	33.51504377313188	37.0	33.0	40.0	18.0	41.0
50	33.42933705992472	37.0	33.0	40.0	17.0	41.0
51	32.400355758180815	35.0	31.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	4.0
10	6.0
11	8.0
12	14.0
13	13.0
14	16.0
15	47.0
16	56.0
17	138.0
18	305.0
19	531.0
20	1001.0
21	1512.0
22	2453.0
23	4180.0
24	7184.0
25	13472.0
26	20866.0
27	24099.0
28	23152.0
29	21628.0
30	22271.0
31	24999.0
32	30246.0
33	40985.0
34	61966.0
35	71955.0
36	93633.0
37	126034.0
38	198343.0
39	163430.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.947928986644403	4.694834697107946	41.45022790103721	27.907008415210445
2	33.693107237625725	4.726785888258828	42.88069844256276	18.69940843155269
3	21.124870492917836	5.2948885427218855	54.17476358737498	19.405477376985296
4	16.314382959644075	5.041688447601618	55.58857760630056	23.055350986453742
5	15.206357553733	6.023480458965766	55.508123459402604	23.26203852789863
6	18.823756182031698	7.007577146412928	57.89084425522822	16.277822416327165
7	69.44575682943615	2.582808583032765	22.603320200171595	5.36811438735948
8	68.92217632657679	3.1013607017110125	21.790502848893915	6.185960122818283
9	62.47316885628354	5.751528681170063	24.996831070385856	6.77847139216054
10	29.854459705357637	24.185166057149683	29.9006579850217	16.059716252470977
11	24.355921603300295	18.972617305393673	36.774982950634886	19.896478140671142
12	20.228665770636542	18.300908985198742	40.46089331340138	21.00953193076334
13	21.412535971279546	18.198560415512148	43.55764466294636	16.831258950261947
14	18.10176402002554	19.394582544592865	44.1120240189151	18.391629416466493
15	15.079705127170978	18.391524658462718	45.37404369037306	21.15472652399325
16	17.18523624501221	19.921620061576757	42.04661521651908	20.846528476891958
17	16.824868712031773	20.583062097401896	40.78281465899698	21.809254531569348
18	18.936371036088083	17.76486227989034	42.52399743971439	20.774769244307187
19	17.41654191734384	22.331263664372116	40.818222864272386	19.433971554011656
20	18.329088888213782	25.076656669261173	37.989337730375944	18.604916712149098
21	16.71277764799425	26.997394668446155	38.56854473323898	17.721282950320614
22	16.151903295791556	22.64197590356397	38.46294866543541	22.74317213520906
23	14.669996574413277	25.541991722022544	37.12770314933987	22.660308554224315
24	15.13396977312559	23.936994346210536	38.27972691683576	22.64930896382811
25	16.813240573612926	25.688548169301505	34.51116248909207	22.987048767993496
26	16.271432178096987	24.662757796352537	36.1904332895794	22.87537673597107
27	17.299946259144065	23.419280291562476	37.25383178588302	22.026941663410437
28	14.910730467084512	23.807303937539086	36.45484249110343	24.827123104272975
29	16.925017363639125	22.56665489885091	35.222364576709566	25.285963160800396
30	20.84118581869951	22.79356073502406	34.53368545990335	21.83156798637308
31	19.646316027660305	26.535726145816856	30.19754216771547	23.620415658807374
32	17.716045050131942	24.822199478095623	30.416172121590517	27.04558335018191
33	18.588679221564224	24.949061420665192	28.990310932231	27.471948425539583
34	16.656732115975494	25.150930093936502	30.525434719526157	27.666903070561847
35	15.062001024533277	27.523594121399857	29.42966600005657	27.984738854010295
36	16.075953743055855	31.41346831751313	25.87417935198794	26.63639858744308
37	15.577410403098323	29.408295367286797	27.520032349271567	27.494261880343313
38	16.13964660935007	27.09963848012898	30.245835607454996	26.514879303065953
39	17.45896890887206	26.772479234344704	28.8619823776086	26.90656947917463
40	19.84870849095048	27.66700782856562	28.566041016948795	23.9182426635351
41	16.399551216711835	26.735709175020244	26.739375705152312	30.12536390311561
42	18.544157069960537	23.936994346210536	28.647123711869398	28.871724871959533
43	17.895914542610843	22.055226324429253	32.965667659423346	27.083191473536555
44	19.770873294146856	23.256172079687317	30.965627851381917	26.007326774783913
45	21.178192316838487	22.24096226511946	30.015053725142238	26.565791692899815
46	20.82997671229576	23.554941906449006	30.80210060749166	24.812980773763567
47	17.087287511484096	26.21789036236841	32.72849553888041	23.966326587267083
48	17.38270508212504	24.495144990315122	33.535760715958105	24.586389211601738
49	21.800245343244836	21.075005683121706	32.64573671589944	24.47901225773402
50	19.106707550223607	20.83615743451839	32.58811981382407	27.469015201433926
51	18.048127922093567	19.459846780943682	30.479236439862095	32.01278885710065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	920.0
1	1876.5
2	2833.0
3	23697.0
4	44561.0
5	28147.0
6	11733.0
7	11146.5
8	10560.0
9	10640.0
10	10720.0
11	10539.0
12	10358.0
13	9925.0
14	9492.0
15	8949.0
16	8406.0
17	7863.5
18	7321.0
19	6944.5
20	6568.0
21	6229.0
22	5890.0
23	5938.5
24	5987.0
25	6061.0
26	6490.5
27	6846.0
28	7483.5
29	8121.0
30	8868.5
31	9616.0
32	10770.0
33	11924.0
34	13403.5
35	14883.0
36	16036.5
37	17190.0
38	18952.0
39	20714.0
40	23891.5
41	27069.0
42	32736.5
43	38404.0
44	46389.0
45	54374.0
46	69765.5
47	85157.0
48	99958.0
49	114759.0
50	115840.5
51	116922.0
52	101760.5
53	86599.0
54	71222.0
55	55845.0
56	46446.5
57	37048.0
58	32879.5
59	28711.0
60	25105.5
61	21500.0
62	19174.0
63	16848.0
64	14841.0
65	12834.0
66	10774.0
67	8714.0
68	7336.5
69	5959.0
70	5105.5
71	4252.0
72	3693.0
73	3134.0
74	2812.5
75	2020.5
76	1550.0
77	1252.0
78	954.0
79	682.5
80	411.0
81	286.0
82	161.0
83	106.5
84	52.0
85	36.5
86	21.0
87	20.0
88	19.0
89	11.5
90	4.0
91	5.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	954581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.80228825223162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80783024215964	28.25110416075825
2	9.743377352844602	7.561306731906835
3	4.604691218751996	5.360176679476041
4	2.7729903377594556	4.303934816255821
5	1.9128967746227539	3.711238602283812
6	1.4410936962635696	3.3550639800535764
7	1.106604160525565	3.005714153248223
8	0.911931186918306	2.8308013424803056
9	0.7019840355425581	2.451472820602843
>10	3.744796837924918	23.344396949130086
>50	0.16083043924133364	4.322595694081962
>100	0.08582937032983541	6.355029724656186
>500	0.0032490613361351233	0.7990801136605767
>1k	0.0016245306680675617	1.3708121644810944
>5k	0.0	0.0
>10k+	2.707551113445936E-4	2.977272066924413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28339	2.968737068933909	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3836	0.4018517024746983	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	2469	0.25864751131648334	RNA PCR Primer, Index 29 (96% over 26bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1962	0.20553520340337805	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1771	0.1855264246826618	RNA PCR Primer, Index 29 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1563	0.1637367598977981	Illumina PCR Primer Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1447	0.1515848314600856	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.095160075467666E-4	0.0	0.0	0.2822180621654946	0.0
2	2.095160075467666E-4	0.0	0.0	0.843720962390829	0.0
3	2.095160075467666E-4	0.0	0.0	1.272809745846607	0.0
4	2.095160075467666E-4	0.0	0.0	1.9632697487169763	0.0
5	2.095160075467666E-4	0.0	0.0	3.5712003486346364	0.0
6	2.095160075467666E-4	0.0	0.0	5.768604235785125	0.0
7	2.095160075467666E-4	0.0	0.0	7.737321400698317	0.0
8	2.095160075467666E-4	0.0	0.0	10.754037635360435	0.0
9	3.1427401132014986E-4	0.0	0.0	12.611606558270068	0.0
10	3.1427401132014986E-4	0.0	0.0	15.454214990660825	0.0
11	3.1427401132014986E-4	0.0	0.0	20.114479546523555	0.0
12	3.1427401132014986E-4	0.0	0.0	23.92159491965585	0.0
13	3.1427401132014986E-4	0.0	0.0	25.337608856660673	0.0
14	4.190320150935332E-4	0.0	0.0	26.0097362088707	0.0
15	4.190320150935332E-4	0.0	0.0	26.728271356752334	0.0
16	4.190320150935332E-4	0.0	0.0	28.153084966074122	0.0
17	4.190320150935332E-4	0.0	0.0	29.796109497255863	0.0
18	4.190320150935332E-4	0.0	0.0	31.33584263671705	0.0
19	4.190320150935332E-4	0.0	0.0	32.27782660664732	0.0
20	4.190320150935332E-4	0.0	0.0	33.172669474879555	0.0
21	4.190320150935332E-4	0.0	0.0	34.145557055922964	0.0
22	4.190320150935332E-4	0.0	0.0	35.03631436200804	0.0
23	4.190320150935332E-4	0.0	0.0	35.67837616713511	0.0
24	5.237900188669165E-4	0.0	0.0	36.091646492021106	0.0
25	5.237900188669165E-4	0.0	0.0	36.40759663140163	0.0
26	5.237900188669165E-4	0.0	0.0	36.67630091108036	0.0
27	5.237900188669165E-4	0.0	0.0	36.9665853395364	0.0
28	6.285480226402997E-4	0.0	0.0	37.223975754807604	0.0
29	6.285480226402997E-4	0.0	0.0	37.496975112641046	0.0
30	6.285480226402997E-4	0.0	0.0	37.83785765691963	0.0
31	6.285480226402997E-4	0.0	0.0	38.06727768518334	0.0
32	6.285480226402997E-4	0.0	0.0	38.44461601477507	0.0
33	6.285480226402997E-4	0.0	0.0	38.705463444170796	0.0
34	6.285480226402997E-4	0.0	0.0	38.96013015134389	0.0
35	6.285480226402997E-4	0.0	0.0	39.249471757765974	0.0
36	6.285480226402997E-4	0.0	0.0	39.46925404968253	0.0
37	6.285480226402997E-4	0.0	0.0	39.688617309584	0.0
38	6.285480226402997E-4	0.0	0.0	39.96549271355705	0.0
39	6.285480226402997E-4	0.0	0.0	40.55381366274837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	30	2.165114E-6	44.999996	9
CGTTTTT	12960	0.0	43.90625	1
CGAACGA	50	1.0804797E-9	40.5	22
GATAACG	50	1.0804797E-9	40.5	17
CGCACGG	45	1.927765E-8	40.000004	2
GTTTTTT	15005	0.0	39.496834	2
CGTTCAT	40	3.4574077E-7	39.375	17
GCTACGA	110	0.0	38.863636	10
TTGGGAC	1685	0.0	38.590504	5
TCGTTAG	35	6.247177E-6	38.571426	1
GCGTACG	35	6.247177E-6	38.571426	1
AACACGT	550	0.0	38.045456	41
CGAGACA	730	0.0	37.91096	22
CGTTTGG	375	0.0	37.8	2
TCTGGGA	2120	0.0	37.78302	4
AGGCACG	215	0.0	37.67442	10
ACGACCA	705	0.0	37.659573	28
GCGAGAC	735	0.0	37.65306	21
ACACGAC	720	0.0	37.500004	26
CACGACC	720	0.0	37.500004	27
>>END_MODULE