SRR2933699.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933699.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	964587
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28163	2.919695164873671	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3965	0.41105675278642567	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	2505	0.2596966370063043	RNA PCR Primer, Index 29 (96% over 26bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1954	0.20257374399613512	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1763	0.18277252337010555	RNA PCR Primer, Index 29 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1580	0.16380067324150127	Illumina PCR Primer Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1506	0.15612899614031703	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	30	2.1651376E-6	45.000004	6
TCCGATA	30	2.1651376E-6	45.000004	14
GTTACGT	20	7.032723E-4	45.0	22
TATTACG	20	7.032723E-4	45.0	39
CGCACGG	55	1.8189894E-12	45.0	2
CGCGTAG	20	7.032723E-4	45.0	1
CGTTTTT	13355	0.0	43.803818	1
CAACCCG	75	0.0	42.0	23
CGAATAT	130	0.0	41.53846	14
TACGCGG	60	3.6379788E-12	41.250004	2
CTACGAA	150	0.0	40.5	11
TTGTGCG	135	0.0	40.0	1
CGCTTGG	220	0.0	39.886364	2
CGTTTGG	540	0.0	39.166664	2
GTTTTTT	15535	0.0	39.01835	2
TACGAAT	150	0.0	39.0	12
CGAACGA	35	6.2472445E-6	38.571426	22
CGAGGGT	35	6.2472445E-6	38.571426	4
ACGAACG	35	6.2472445E-6	38.571426	21
GACCTAT	135	0.0	38.333332	33