##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933699.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	964587
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973019541005634	33.0	31.0	34.0	30.0	34.0
2	32.169970153029226	34.0	31.0	34.0	30.0	34.0
3	32.31426817902377	34.0	31.0	34.0	30.0	34.0
4	35.83817530196861	37.0	35.0	37.0	35.0	37.0
5	35.91726096246373	37.0	35.0	37.0	35.0	37.0
6	35.897036762884014	37.0	35.0	37.0	35.0	37.0
7	35.88787636573995	37.0	35.0	37.0	35.0	37.0
8	35.74864579348467	37.0	35.0	37.0	35.0	37.0
9	37.55040965719007	39.0	37.0	39.0	35.0	39.0
10	37.10370241357182	39.0	37.0	39.0	33.0	39.0
11	37.10624650757267	39.0	37.0	39.0	33.0	39.0
12	37.02237330588117	39.0	37.0	39.0	33.0	39.0
13	36.96285560555968	39.0	37.0	39.0	33.0	39.0
14	37.940948820583316	40.0	37.0	41.0	33.0	41.0
15	38.000614770881214	40.0	37.0	41.0	33.0	41.0
16	38.17870964464584	40.0	37.0	41.0	33.0	41.0
17	38.1868177779713	40.0	37.0	41.0	33.0	41.0
18	38.05131107924946	39.0	37.0	41.0	34.0	41.0
19	37.84279074878679	39.0	37.0	41.0	34.0	41.0
20	37.467004013116494	39.0	35.0	41.0	33.0	41.0
21	37.36324147018361	39.0	35.0	40.0	33.0	41.0
22	37.39405154744984	39.0	35.0	40.0	33.0	41.0
23	37.26029274705133	39.0	35.0	40.0	33.0	41.0
24	37.170829588207184	39.0	35.0	40.0	33.0	41.0
25	37.09904757165502	39.0	35.0	40.0	33.0	41.0
26	37.09831150533856	39.0	35.0	40.0	33.0	41.0
27	37.03015487457326	39.0	35.0	40.0	33.0	41.0
28	36.97667706489928	39.0	35.0	40.0	33.0	41.0
29	36.824118508750374	39.0	35.0	40.0	32.0	41.0
30	36.58630170217928	38.0	35.0	40.0	31.0	41.0
31	36.32518165805676	38.0	35.0	40.0	31.0	41.0
32	36.094812598552544	38.0	35.0	40.0	30.0	41.0
33	35.65636692180177	38.0	35.0	40.0	27.0	41.0
34	35.327656292278455	38.0	35.0	40.0	24.0	41.0
35	35.10060886161642	38.0	35.0	40.0	23.0	41.0
36	34.90860959146246	38.0	35.0	40.0	22.0	41.0
37	34.82357319764832	38.0	34.0	40.0	22.0	41.0
38	34.74976440694307	38.0	34.0	40.0	21.0	41.0
39	34.71682492092471	38.0	34.0	40.0	21.0	41.0
40	34.53887518699713	38.0	34.0	40.0	20.0	41.0
41	34.43912161370618	38.0	34.0	40.0	19.0	41.0
42	34.31028201707052	38.0	34.0	40.0	18.0	41.0
43	34.23843987115729	38.0	34.0	40.0	18.0	41.0
44	34.13160243710521	38.0	34.0	40.0	18.0	41.0
45	34.02246972020149	38.0	33.0	40.0	18.0	41.0
46	33.79190990548287	37.0	33.0	40.0	18.0	41.0
47	33.75591729932085	37.0	33.0	40.0	18.0	41.0
48	33.67815137462976	37.0	33.0	40.0	18.0	41.0
49	33.71295798098046	37.0	33.0	40.0	18.0	41.0
50	33.54131871982517	37.0	33.0	40.0	18.0	41.0
51	32.68161606988276	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	4.0
9	6.0
10	14.0
11	18.0
12	15.0
13	16.0
14	29.0
15	40.0
16	67.0
17	149.0
18	300.0
19	541.0
20	969.0
21	1524.0
22	2598.0
23	4121.0
24	7210.0
25	13278.0
26	20710.0
27	23754.0
28	22899.0
29	21477.0
30	21850.0
31	24405.0
32	29366.0
33	39354.0
34	60430.0
35	70035.0
36	90049.0
37	118522.0
38	199442.0
39	191337.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.077689207920073	4.85741566079576	41.179385581601245	27.88550954968292
2	33.76170319525351	4.799670739912522	42.8081655672324	18.630460497601565
3	21.2934654935221	5.439633749988337	53.94567830584488	19.32122245064468
4	16.521578665273324	5.139919986481261	55.251211140104516	23.087290208140892
5	15.287475365104445	6.1171257750726475	55.33435553247141	23.261043327351498
6	18.78503442405921	7.118901664650259	57.738493261883065	16.357570649407467
7	69.38938633840182	2.604223361915514	22.56934833249878	5.437041967183883
8	68.95614392480927	3.118018385070502	21.74837521135989	6.17746247876034
9	62.5903106718212	5.803416384421519	24.82337000187645	6.782902941880825
10	29.99221428445542	24.125869413541754	29.81483266931858	16.06708363268425
11	24.370948395530938	19.063288225945403	36.62043962856642	19.945323749957236
12	20.274583837435088	18.441053010252055	40.357894103901465	20.92646904841139
13	21.403357084430954	18.36018938675309	43.32600377156234	16.910449757253623
14	18.178971933065654	19.53841384965794	43.87566906873097	18.40694514854544
15	15.146793394478673	18.433070319214337	45.312035098959456	21.10810118734754
16	17.250284318573648	20.009392620883343	41.907158193091966	20.833164867451043
17	16.878518993102748	20.654435525255888	40.71701152928663	21.750033952354737
18	19.03032074867275	17.800778986239706	42.40840898747339	20.76049127761415
19	17.394076428564766	22.34852843755929	40.697832336533665	19.55956279734228
20	18.61262903190692	25.08151156920008	37.80322562920711	18.502633769685886
21	16.8597544855985	27.014048499513265	38.44453636634124	17.681660648546995
22	16.263644440574048	22.70785320556881	38.36978935026079	22.658713003596358
23	14.870094662275148	25.618632637595155	36.99687016308534	22.51440253704435
24	15.242793029555655	23.920911229365522	38.26456296839994	22.571732772678878
25	16.822225470590006	25.799228063409522	34.46438734919712	22.914159116803358
26	16.414693542417634	24.751836796473516	36.14800945897053	22.685460202138323
27	17.4845814840963	23.484247662471088	37.09100371454311	21.940167138889493
28	14.966508982600843	23.940297764742837	36.38945994503347	24.703733307622848
29	17.107632593016493	22.570281374308383	35.18749475163982	25.134591281035302
30	20.948240023968808	22.880880625594166	34.46376532132405	21.70711402911298
31	19.74160962152714	26.473506277816313	30.241751132868266	23.543132967788285
32	17.822446290484944	24.90184918519532	30.421413516873024	26.85429100744671
33	18.782235298630397	24.820052519886747	28.991786121936126	27.405926059546726
34	16.763132822648448	25.2680162598086	30.52156000443713	27.447290913105814
35	15.23170019915259	27.530538976784886	29.4495986365149	27.78816218754762
36	16.16204655463945	31.445478738568944	25.954838702988948	26.437636003802663
37	15.692311839160181	29.375162634370977	27.60145015431475	27.331075372154096
38	16.180603719519336	27.242021714993047	30.145751497791284	26.43162306769633
39	17.576123252749625	26.855120377944136	28.918075818977446	26.650680550328794
40	19.99995853147513	27.67028790560105	28.472289176611337	23.857464386312486
41	16.49597185116532	26.77342738394774	26.673902924256705	30.056697840630235
42	18.665916086366497	23.902665078422164	28.734370253797742	28.697048581413597
43	17.98873507521872	22.181824967576798	32.87894197205644	26.950497985148047
44	19.767423778259506	23.394675648749153	30.916236689899407	25.921663883091934
45	21.236446271824107	22.419854300337867	30.019168825621744	26.324530602216285
46	20.77853008593315	23.699572977865138	30.839934604136275	24.68196233206543
47	17.09778381835957	26.308357877516492	32.72167259148216	23.872185712641784
48	17.60276677997941	24.527181062983434	33.499622118067116	24.370430038970046
49	21.864487080999435	21.260187002312907	32.581094292168565	24.294231624519096
50	19.107970561494195	20.958399812562266	32.6008955127946	27.332734113148945
51	18.097071596444902	19.63420614211056	30.472419802464685	31.796302458979852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	905.0
1	1844.5
2	2784.0
3	23601.5
4	44419.0
5	28216.0
6	12013.0
7	11387.0
8	10761.0
9	10923.5
10	11086.0
11	10791.5
12	10497.0
13	9992.0
14	9487.0
15	8916.5
16	8346.0
17	7850.5
18	7355.0
19	6999.0
20	6643.0
21	6277.5
22	5912.0
23	5946.5
24	5981.0
25	6148.5
26	6491.5
27	6667.0
28	7464.5
29	8262.0
30	9184.5
31	10107.0
32	11196.5
33	12286.0
34	14003.0
35	15720.0
36	16739.5
37	17759.0
38	19530.5
39	21302.0
40	24575.0
41	27848.0
42	33749.0
43	39650.0
44	46940.0
45	54230.0
46	69570.0
47	84910.0
48	100093.0
49	115276.0
50	116242.0
51	117208.0
52	102312.0
53	87416.0
54	71818.5
55	56221.0
56	47076.5
57	37932.0
58	33253.0
59	28574.0
60	25191.0
61	21808.0
62	19603.5
63	17399.0
64	15177.0
65	12955.0
66	10926.0
67	8897.0
68	7675.5
69	6454.0
70	5321.5
71	4189.0
72	3753.0
73	3317.0
74	2905.0
75	2046.0
76	1599.0
77	1264.0
78	929.0
79	666.5
80	404.0
81	281.0
82	158.0
83	108.5
84	59.0
85	40.5
86	22.0
87	20.5
88	19.0
89	13.0
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	964587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.69254638575859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62099289679811	28.09891136239206
2	9.975753815835303	7.719746345042314
3	4.529232745783064	5.257426443243237
4	2.7117827626776125	4.197031213320163
5	1.8938382446368998	3.663871206386843
6	1.4588007304603308	3.3866828958548916
7	1.1370783742855288	3.0797520418900057
8	0.9252985374179065	2.864172526377354
9	0.7310332893899427	2.5456985513328636
>10	3.766324652648538	23.56618002211041
>50	0.1608202707368597	4.336560456710065
>100	0.08418675136831687	6.2205895448548185
>500	0.0029681226432126135	0.7372999119882189
>1k	0.0016189759872068802	1.3857521568705222
>5k	0.0	0.0
>10k+	2.6982933120114666E-4	2.9403253216261973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28163	2.919695164873671	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3965	0.41105675278642567	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	2505	0.2596966370063043	RNA PCR Primer, Index 29 (96% over 26bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1954	0.20257374399613512	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1763	0.18277252337010555	RNA PCR Primer, Index 29 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1580	0.16380067324150127	Illumina PCR Primer Index 8 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1506	0.15612899614031703	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.146852487126615E-4	0.0	0.0	0.2779427879496614	0.0
2	4.146852487126615E-4	0.0	0.0	0.8283337843035413	0.0
3	4.146852487126615E-4	0.0	0.0	1.2436410608892718	0.0
4	4.146852487126615E-4	0.0	0.0	1.9115953252531912	0.0
5	4.146852487126615E-4	0.0	0.0	3.4840817883716038	0.0
6	4.146852487126615E-4	0.0	0.0	5.608721660150925	0.0
7	4.146852487126615E-4	0.0	0.0	7.573085683302802	0.0
8	4.146852487126615E-4	0.0	0.0	10.529791506624079	0.0
9	5.183565608908268E-4	0.0	0.0	12.341862372186231	0.0
10	5.183565608908268E-4	0.0	0.0	15.163484475739358	0.0
11	5.183565608908268E-4	0.0	0.0	19.766594407762078	0.0
12	5.183565608908268E-4	0.0	0.0	23.491193640387024	0.0
13	5.183565608908268E-4	0.0	0.0	24.877279084209096	0.0
14	5.183565608908268E-4	0.0	0.0	25.552075655176775	0.0
15	5.183565608908268E-4	0.0	0.0	26.265023269026017	0.0
16	5.183565608908268E-4	0.0	0.0	27.642400322625125	0.0
17	6.220278730689923E-4	0.0	0.0	29.250549717132824	0.0
18	6.220278730689923E-4	0.0	0.0	30.76974912579166	0.0
19	6.220278730689923E-4	0.0	0.0	31.698436740283665	0.0
20	6.220278730689923E-4	0.0	0.0	32.571660202760356	0.0
21	6.220278730689923E-4	0.0	0.0	33.52761337235521	0.0
22	6.220278730689923E-4	0.0	0.0	34.36227110670163	0.0
23	6.220278730689923E-4	0.0	0.0	34.99217799949616	0.0
24	6.220278730689923E-4	0.0	0.0	35.39442269074744	0.0
25	6.220278730689923E-4	0.0	0.0	35.697972292805105	0.0
26	6.220278730689923E-4	0.0	0.0	35.95435144782171	0.0
27	6.220278730689923E-4	0.0	0.0	36.23053182346434	0.0
28	6.220278730689923E-4	0.0	0.0	36.48483755223738	0.0
29	6.220278730689923E-4	0.0	0.0	36.76081058525566	0.0
30	6.220278730689923E-4	0.0	0.0	37.107487453179445	0.0
31	6.220278730689923E-4	0.0	0.0	37.34105891951685	0.0
32	6.220278730689923E-4	0.0	0.0	37.722776690956856	0.0
33	6.220278730689923E-4	0.0	0.0	37.96972175656524	0.0
34	6.220278730689923E-4	0.0	0.0	38.208269445887204	0.0
35	7.256991852471576E-4	0.0	0.0	38.492432512567554	0.0
36	7.256991852471576E-4	0.0	0.0	38.700500836109136	0.0
37	7.256991852471576E-4	0.0	0.0	38.90794713177764	0.0
38	8.29370497425323E-4	0.0	0.0	39.19812313456433	0.0
39	8.29370497425323E-4	0.0	0.0	39.77412094502621	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	30	2.1651376E-6	45.000004	6
TCCGATA	30	2.1651376E-6	45.000004	14
GTTACGT	20	7.032723E-4	45.0	22
TATTACG	20	7.032723E-4	45.0	39
CGCACGG	55	1.8189894E-12	45.0	2
CGCGTAG	20	7.032723E-4	45.0	1
CGTTTTT	13355	0.0	43.803818	1
CAACCCG	75	0.0	42.0	23
CGAATAT	130	0.0	41.53846	14
TACGCGG	60	3.6379788E-12	41.250004	2
CTACGAA	150	0.0	40.5	11
TTGTGCG	135	0.0	40.0	1
CGCTTGG	220	0.0	39.886364	2
CGTTTGG	540	0.0	39.166664	2
GTTTTTT	15535	0.0	39.01835	2
TACGAAT	150	0.0	39.0	12
CGAACGA	35	6.2472445E-6	38.571426	22
CGAGGGT	35	6.2472445E-6	38.571426	4
ACGAACG	35	6.2472445E-6	38.571426	21
GACCTAT	135	0.0	38.333332	33
>>END_MODULE