##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933697.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821166
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27870247915769	33.0	31.0	34.0	30.0	34.0
2	32.51256871326869	34.0	31.0	34.0	31.0	34.0
3	32.566638170601315	34.0	31.0	34.0	31.0	34.0
4	36.06912487852639	37.0	35.0	37.0	35.0	37.0
5	36.13913508352757	37.0	35.0	37.0	35.0	37.0
6	36.135173643331555	37.0	36.0	37.0	35.0	37.0
7	36.137828404975366	37.0	36.0	37.0	35.0	37.0
8	36.040287834615654	37.0	37.0	37.0	35.0	37.0
9	37.85586227388859	39.0	38.0	39.0	35.0	39.0
10	37.347418183412366	39.0	37.0	39.0	34.0	39.0
11	37.28051210108553	39.0	37.0	39.0	34.0	39.0
12	37.39929076459571	39.0	37.0	39.0	34.0	39.0
13	37.45289381196981	39.0	37.0	39.0	35.0	39.0
14	38.71142497375683	40.0	38.0	41.0	35.0	41.0
15	38.72536490794797	40.0	38.0	41.0	34.0	41.0
16	38.8320692771011	40.0	38.0	41.0	35.0	41.0
17	38.838535691930744	40.0	38.0	41.0	35.0	41.0
18	38.40757654359776	39.0	38.0	40.0	35.0	41.0
19	37.93508377112545	38.0	37.0	40.0	35.0	41.0
20	37.28440047444731	38.0	35.0	40.0	34.0	41.0
21	37.20002167649415	38.0	35.0	40.0	34.0	41.0
22	37.189934800028254	38.0	35.0	40.0	34.0	41.0
23	37.05630652024073	38.0	35.0	40.0	33.0	41.0
24	36.9434523592063	38.0	35.0	40.0	33.0	41.0
25	36.88449594844404	38.0	35.0	40.0	33.0	41.0
26	36.888346570608135	38.0	35.0	40.0	33.0	41.0
27	36.817830986670174	38.0	35.0	40.0	33.0	41.0
28	36.72383537555135	38.0	35.0	40.0	33.0	41.0
29	36.52173007650097	38.0	35.0	40.0	33.0	41.0
30	36.18069671662003	38.0	35.0	40.0	31.0	41.0
31	35.66789053614007	38.0	35.0	40.0	30.0	41.0
32	34.95944425365882	38.0	35.0	40.0	23.0	41.0
33	33.92185014966523	38.0	34.0	40.0	16.0	41.0
34	33.10824607935545	38.0	33.0	40.0	10.0	41.0
35	32.59407963797819	38.0	32.0	40.0	8.0	41.0
36	32.24349400730181	38.0	31.0	40.0	8.0	41.0
37	32.05568910549146	38.0	30.0	40.0	7.0	41.0
38	31.882562112898	38.0	30.0	40.0	7.0	41.0
39	31.82931343966993	38.0	30.0	40.0	7.0	41.0
40	31.683174900081106	38.0	29.0	40.0	7.0	41.0
41	31.458328766656194	37.0	27.0	40.0	7.0	41.0
42	31.201752142684914	37.0	26.0	40.0	7.0	41.0
43	31.11225379521315	37.0	25.0	40.0	7.0	41.0
44	31.036349532250483	37.0	24.0	40.0	7.0	41.0
45	31.01025614796521	37.0	24.0	40.0	7.0	41.0
46	30.75921068334539	37.0	23.0	40.0	7.0	41.0
47	30.649908788235265	36.0	23.0	40.0	7.0	41.0
48	30.50193261776547	36.0	23.0	40.0	7.0	41.0
49	30.49486827267568	36.0	23.0	40.0	7.0	41.0
50	30.329609360348577	36.0	23.0	40.0	7.0	41.0
51	29.419395104034994	35.0	21.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	7.0
12	9.0
13	16.0
14	14.0
15	33.0
16	46.0
17	137.0
18	307.0
19	605.0
20	1160.0
21	2036.0
22	3474.0
23	6105.0
24	12176.0
25	25615.0
26	39777.0
27	40874.0
28	32224.0
29	24424.0
30	20963.0
31	20361.0
32	21741.0
33	25548.0
34	35291.0
35	48167.0
36	64550.0
37	82740.0
38	149620.0
39	163068.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.834596171785	4.074693789075534	40.81720382967634	41.27350620946313
2	41.94730419915096	3.804833614640645	39.14007642790861	15.107785758299782
3	13.54598217656357	3.7127694035067207	66.33421257090527	16.40703584902444
4	12.140663398143616	3.8596337403156005	65.9899460036095	18.009756857931283
5	11.767413653268646	4.284030269153861	66.03220298940774	17.91635308816975
6	14.815031309138469	4.895234337515192	67.80590039042045	12.483833962925889
7	59.92917388201655	1.3310341636161265	34.932766334699686	3.8070256196676437
8	60.680909828219875	1.7539693557697227	33.399824152485614	4.165296663524793
9	55.23950090481097	4.666048034136825	35.542630844433404	4.5518202166188075
10	23.409761241941336	23.258634673135518	41.45215461916349	11.879449465759663
11	15.505391114585846	17.77825677146886	50.00840268593683	16.70794942800847
12	13.96258490975028	15.527676499026994	53.231746078137675	17.277992513085053
13	16.402895395084553	15.807644252197486	54.53647618142982	13.252984171288142
14	14.997211282493431	17.736121563727675	53.09072708806746	14.17594006571144
15	11.668286314825504	18.145904725719284	54.222045238112635	15.963763721342577
16	12.277785490388057	18.34744741014606	52.387580586629255	16.987186512836626
17	12.629724075278324	18.067114322804404	50.87278333491645	18.430378267000826
18	13.46767888587691	17.349719788690717	52.63637315719355	16.546228168238823
19	13.406424523178018	19.766405330956225	52.37942145680654	14.447748689059217
20	13.62525969170667	21.567259238692298	50.002192005027	14.805289064574032
21	13.025746316822664	22.279782650523767	50.22650718612315	14.467963846530422
22	12.239303624358534	20.57976097402961	49.32449711751339	17.856438284098463
23	11.268854287683611	21.690011520204198	48.035719939695504	19.005414252416685
24	11.58803457522596	21.34233516731087	48.76943273345463	18.300197524008542
25	11.529946442010507	22.710146304157746	47.19411178738525	18.56579546644649
26	11.721503325758738	23.005458092517227	48.067869346758144	17.20516923496589
27	12.288501959408938	22.057172362226396	49.423380899842414	16.230944778522247
28	11.293697011322925	20.862651400569433	49.375765679533735	18.467885908573905
29	12.43475740593254	18.862325035376525	48.53233085636765	20.17058670232328
30	15.793761553693164	20.08595095266974	46.194801051188186	17.925486442448907
31	14.645272697603165	23.92208152797364	43.04148004179423	18.39116573262897
32	13.313751421758816	23.866916068127516	41.78083359515616	21.03849891495751
33	15.397495756034713	22.304990708334245	38.85925622833873	23.43825730729231
34	15.35438632383708	22.718792546208682	38.04784903417823	23.878972095776007
35	14.510099054271608	22.54172725125005	38.53410881600066	24.414064878477674
36	14.999037953349262	25.15264879451901	36.57153364849495	23.27677960363678
37	15.604274896914875	28.14802366391205	34.576565517812476	21.6711359213606
38	15.31773112866339	27.084535891646755	34.44711544316253	23.150617536527328
39	17.1529264484891	24.7215544725427	33.209485049307936	24.916034029660263
40	20.31099680210822	22.705640516046692	35.389312270600584	21.5940504112445
41	18.71753092553759	24.009639950996508	36.905449080941004	20.367380042524896
42	17.541642006610115	26.98784411434472	36.786837253368994	18.683676625676174
43	16.37963578618696	26.4928163124143	37.14133317745742	19.986214723941323
44	16.99534564265934	24.731053160993028	34.738895667867396	23.534705528480234
45	19.398757376705806	22.45124615485785	33.815452661215886	24.334543807220467
46	20.399894783758704	22.970629568199367	35.48320315259034	21.14627249545159
47	17.04515286799502	25.79551515771476	37.09590996217574	20.063422012114483
48	16.257735951074448	25.327034972222425	37.602141345355264	20.813087731347863
49	19.236427226650886	22.468295082845614	37.08580238344013	21.20947530706337
50	18.707545124859042	20.902229269112457	36.07236539262463	24.317860213403865
51	17.337663761042226	20.487696762895688	34.91425607002726	27.260383406034833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	654.0
1	1370.0
2	2086.0
3	29783.5
4	57481.0
5	38564.5
6	19648.0
7	19450.5
8	19253.0
9	19439.5
10	19626.0
11	19557.5
12	19489.0
13	19152.5
14	18816.0
15	17953.0
16	17090.0
17	16134.0
18	15178.0
19	14317.5
20	13457.0
21	12584.5
22	11712.0
23	11170.0
24	10628.0
25	10441.0
26	10169.0
27	10084.0
28	10223.0
29	10362.0
30	11131.5
31	11901.0
32	13017.0
33	14133.0
34	15600.0
35	17067.0
36	18279.0
37	19491.0
38	21322.0
39	23153.0
40	25923.5
41	28694.0
42	33493.5
43	38293.0
44	44149.5
45	50006.0
46	60384.5
47	70763.0
48	79257.0
49	87751.0
50	83876.5
51	80002.0
52	65052.0
53	50102.0
54	39209.5
55	28317.0
56	22184.0
57	16051.0
58	13303.0
59	10555.0
60	8897.5
61	7240.0
62	5926.5
63	4613.0
64	3836.0
65	3059.0
66	2487.5
67	1916.0
68	1438.0
69	960.0
70	717.0
71	474.0
72	411.0
73	348.0
74	256.0
75	167.0
76	170.0
77	108.0
78	46.0
79	49.0
80	52.0
81	32.5
82	13.0
83	12.0
84	11.0
85	5.5
86	0.0
87	0.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.99107949972252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88644930420962	37.378547805094314
2	9.210587882871916	8.840521106521742
3	4.011460216833098	5.775429185280339
4	2.338972039351472	4.489991723525785
5	1.5473314828060962	3.7129054101885446
6	1.049478802607009	3.0219372389511943
7	0.7399495318107354	2.4857683764838017
8	0.5695101012797642	2.1865123637129753
9	0.4339465515466482	1.8743007108515064
>10	2.0558027552882003	16.35722425244773
>50	0.10655028873699793	3.556543246236433
>100	0.04460807380283741	3.6556123484387646
>500	0.0038235491825888775	1.1192048112004083
>1k	0.0012745163941962924	1.0873987940496699
>5k	0.0	0.0
>10k+	2.549032788392585E-4	4.458102627016776
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36443	4.437957733271957	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCC	3377	0.4112444986762725	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC	1769	0.21542538293110042	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	1601	0.1949666693457839	TruSeq Adapter, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTG	1097	0.13359052858983445	No Hit
CTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGCT	1045	0.12725806962295078	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	955	0.11629804448795981	TruSeq Adapter, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4355611411091058E-4	0.0	0.0	0.13870520698616357	0.0
2	2.4355611411091058E-4	0.0	0.0	0.550193261776547	0.0
3	2.4355611411091058E-4	0.0	0.0	0.9437799421797786	0.0
4	2.4355611411091058E-4	0.0	0.0	1.5439022073490622	0.0
5	2.4355611411091058E-4	0.0	0.0	2.817457128035014	0.0
6	2.4355611411091058E-4	0.0	0.0	4.255534203802886	0.0
7	2.4355611411091058E-4	0.0	0.0	5.693976613741923	0.0
8	2.4355611411091058E-4	0.0	0.0	7.930308853508304	0.0
9	2.4355611411091058E-4	0.0	0.0	9.30798401297667	0.0
10	2.4355611411091058E-4	0.0	0.0	11.281032093389156	0.0
11	2.4355611411091058E-4	0.0	0.0	15.416127798764196	0.0
12	2.4355611411091058E-4	0.0	0.0	18.952440797597564	0.0
13	2.4355611411091058E-4	0.0	0.0	20.21491391509147	0.0
14	2.4355611411091058E-4	0.0	0.0	20.663544277283766	0.0
15	2.4355611411091058E-4	0.0	0.0	21.189990817934497	0.0
16	2.4355611411091058E-4	0.0	0.0	22.42384609202037	0.0
17	2.4355611411091058E-4	0.0	0.0	24.121675763487527	0.0
18	2.4355611411091058E-4	0.0	0.0	25.77310799521656	0.0
19	2.4355611411091058E-4	0.0	0.0	26.78484009323328	0.0
20	2.4355611411091058E-4	0.0	0.0	27.716685785821625	0.0
21	2.4355611411091058E-4	0.0	0.0	28.80793895509556	0.0
22	2.4355611411091058E-4	0.0	0.0	29.76535803966555	0.0
23	2.4355611411091058E-4	0.0	0.0	30.50893485604616	0.0
24	2.4355611411091058E-4	0.0	0.0	30.951232759271573	0.0
25	2.4355611411091058E-4	0.0	0.0	31.2699259345857	0.0
26	2.4355611411091058E-4	0.0	0.0	31.533453650053705	0.0
27	2.4355611411091058E-4	0.0	0.0	31.787360899014327	0.0
28	3.653341711663659E-4	0.0	0.0	32.01727787073503	0.0
29	3.653341711663659E-4	0.0	0.0	32.252187742795	0.0
30	3.653341711663659E-4	0.0	0.0	32.497083415533524	0.0
31	3.653341711663659E-4	0.0	0.0	32.70422789058485	0.0
32	3.653341711663659E-4	0.0	0.0	32.89724611101775	0.0
33	3.653341711663659E-4	0.0	0.0	33.080400308829155	0.0
34	3.653341711663659E-4	0.0	0.0	33.26367628469761	0.0
35	3.653341711663659E-4	0.0	0.0	33.44634337028079	0.0
36	4.8711222822182116E-4	0.0	0.0	33.6204859918701	0.0
37	6.088902852772765E-4	0.0	0.0	33.80205707493978	0.0
38	6.088902852772765E-4	0.0	0.0	33.98837750223463	0.0
39	7.306683423327318E-4	0.0	0.0	34.21732024949888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0321077E-4	45.000004	1
ATTCGCG	20	7.0321077E-4	45.000004	1
CGCCTCG	20	7.0321077E-4	45.000004	1
CTATGCG	25	3.88983E-5	45.0	1
GATCGGA	25	3.88983E-5	45.0	9
TCGATGA	25	3.88983E-5	45.0	38
TACGAAT	50	2.1827873E-11	45.0	12
TGGTCCG	25	3.88983E-5	45.0	1
CTCGATG	30	2.164741E-6	44.999996	37
CGTTTTT	22495	0.0	44.45988	1
TCTTGCG	130	0.0	43.269234	1
TACGGGA	100	0.0	42.75	4
TCTCTCG	60	3.6379788E-12	41.249996	1
GGCACGT	55	6.002665E-11	40.909092	11
CGAATAT	55	6.002665E-11	40.909092	14
TGTACGG	50	1.0804797E-9	40.5	2
GTTTTTT	25460	0.0	39.95385	2
TTTCGCG	85	0.0	39.705883	1
CTCTCCG	40	3.4566074E-7	39.375004	1
ACGGGTA	35	6.2461113E-6	38.571426	5
>>END_MODULE