FastQCFastQC Report
Sat 14 Jan 2017
SRR2933694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131694
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540842.5371371313143443No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC214031.0040371648088326No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT168370.7898413186883295TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC117500.5512048164511417TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC110480.518273260608699TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC109960.5158338861018513No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC69020.32378005473581106TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG59420.27874544845554755No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG58420.2740543436346868No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC50250.2357280172482542No Hit
TCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG43960.2062209679250399No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT36710.17221045797379925No Hit
TTTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC35930.16855139621352783No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT34940.16390720244087567No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC33910.15907536447538906No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT33580.157527299884505No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT31130.1460340930733961No Hit
GTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC24620.1154950006895924No Hit
TTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG23740.11136682844723493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGG207.034643E-445.0000042
GTCTACG207.034643E-445.0000041
GTCCGGC207.034643E-445.0000043
CGGCTCG207.034643E-445.0000041
GTTAGCG453.8562575E-1045.01
GCGCTAG253.891933E-545.01
TAGTCCG302.16638E-644.9999961
CGTTTTT441000.044.6122441
GCCGATC750.042.09
GTTTTTT484650.040.6453132
CGTGCGG950.040.2631572
TTCGTTG4650.040.1612931
GCGTTAG750.039.01
CGTCCGG750.039.02
CGTTAGG1450.038.7931062
TTCTTCG3200.038.671881
GTTTGCG1750.038.571431
GCGGATA356.2508025E-638.5714265
CGATGCA356.2508025E-638.57142610
TTGGGAC37500.038.2800035
Produced by FastQC (version 0.11.5)