SRR2933693.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933693.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123670
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53468	2.517716971092496	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC	21469	1.0109386109894665	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	16986	0.7998417833279182	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	11825	0.5568190914784312	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	11230	0.5288015557972755	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	11011	0.5184892191347996	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	7137	0.3360691632880815	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	6098	0.28714442451039945	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	5758	0.2711344041211676	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC	4813	0.22663596509815553	No Hit
TCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	4593	0.2162765401404173	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	3707	0.17455631053788961	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	3559	0.1675872428390475	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	3525	0.1659862408001243	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT	3373	0.15882881992023243	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	3260	0.15350784255557595	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	3258	0.15341366596505107	No Hit
GTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	2353	0.11079875875253688	No Hit
TTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	2299	0.10825599080836476	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	35	1.2125747E-7	45.000004	2
AGTTCGG	30	2.1663745E-6	45.000004	2
CTATCCG	20	7.0346385E-4	45.0	1
GGCGTTA	25	3.8919265E-5	45.0	8
ACGGGTA	90	0.0	45.0	5
GCGATAC	20	7.0346385E-4	45.0	9
CGGCTCG	20	7.0346385E-4	45.0	1
CGTTTTT	43130	0.0	44.634827	1
TACCGGG	60	3.6379788E-12	41.250004	3
TCGATTG	55	6.184564E-11	40.909092	1
TTCGTTG	425	0.0	40.764706	1
CCTTACG	50	1.0822987E-9	40.5	1
GTTTTTT	47635	0.0	40.446625	2
TACTGCG	45	1.929584E-8	40.0	1
TGTACGG	90	0.0	40.0	2
TGTACCG	45	1.929584E-8	40.0	1
GTTTGCG	250	0.0	39.6	1
CCGTTTG	195	0.0	39.23077	1
CGTTAGG	115	0.0	39.130432	2
GACGGGA	110	0.0	38.863636	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content