##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933693.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123670
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86401559564339	33.0	31.0	34.0	30.0	34.0
2	32.11856456040722	34.0	31.0	34.0	30.0	34.0
3	32.26128918334769	34.0	31.0	34.0	30.0	34.0
4	35.8287426954282	37.0	35.0	37.0	35.0	37.0
5	35.91453709851342	37.0	35.0	37.0	35.0	37.0
6	35.91926240894301	37.0	35.0	37.0	35.0	37.0
7	35.90089514849294	37.0	35.0	37.0	35.0	37.0
8	35.760518347954246	37.0	35.0	37.0	35.0	37.0
9	37.552905112376216	39.0	37.0	39.0	35.0	39.0
10	36.91390564447396	39.0	37.0	39.0	32.0	39.0
11	36.989534155494965	39.0	37.0	39.0	33.0	39.0
12	37.13964081048374	39.0	37.0	39.0	34.0	39.0
13	37.18455503915392	39.0	37.0	39.0	34.0	39.0
14	38.28691840069314	40.0	38.0	41.0	34.0	41.0
15	38.29175578126545	40.0	38.0	41.0	34.0	41.0
16	38.426919436635636	40.0	38.0	41.0	34.0	41.0
17	38.47432039817863	40.0	38.0	41.0	34.0	41.0
18	38.259572814985376	39.0	38.0	40.0	34.0	41.0
19	37.96980133448229	39.0	37.0	40.0	34.0	41.0
20	37.46004699411867	39.0	35.0	40.0	34.0	41.0
21	37.366619578371406	39.0	35.0	40.0	33.0	41.0
22	37.37863556955647	39.0	35.0	40.0	33.0	41.0
23	37.277886865661806	39.0	35.0	40.0	33.0	41.0
24	37.19339021599401	39.0	35.0	40.0	33.0	41.0
25	37.10127750545047	38.0	35.0	40.0	33.0	41.0
26	37.071281319602384	38.0	35.0	40.0	33.0	41.0
27	36.94896288029685	38.0	35.0	40.0	33.0	41.0
28	36.84958491667726	38.0	35.0	40.0	33.0	41.0
29	36.64732891645124	38.0	35.0	40.0	32.0	41.0
30	36.300534452151226	38.0	35.0	40.0	31.0	41.0
31	35.81527685563199	38.0	35.0	40.0	30.0	41.0
32	35.30634420602071	38.0	35.0	40.0	25.0	41.0
33	34.52799728771419	38.0	34.0	40.0	21.0	41.0
34	33.902530524987405	38.0	33.0	40.0	15.0	41.0
35	33.51942109649804	38.0	33.0	40.0	12.0	41.0
36	33.12964490716543	38.0	32.0	40.0	10.0	41.0
37	32.91467271280378	38.0	31.0	40.0	10.0	41.0
38	32.96217397241568	38.0	32.0	40.0	10.0	41.0
39	33.18461154510823	38.0	33.0	40.0	10.0	41.0
40	33.180054339892735	38.0	33.0	40.0	10.0	41.0
41	33.135443830727	38.0	33.0	40.0	10.0	41.0
42	32.922909868294035	38.0	32.0	40.0	10.0	41.0
43	32.757124223631735	38.0	31.0	40.0	10.0	41.0
44	32.615095565695235	38.0	31.0	40.0	9.0	41.0
45	32.612994015077675	38.0	31.0	40.0	9.0	41.0
46	32.33863500449693	38.0	31.0	40.0	8.0	41.0
47	32.294410148469396	37.0	31.0	40.0	8.0	41.0
48	32.26099346885345	38.0	31.0	40.0	8.0	41.0
49	32.36492345797605	38.0	31.0	40.0	8.0	41.0
50	32.23343268963634	38.0	31.0	40.0	8.0	41.0
51	31.39950651466565	37.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	7.0
11	6.0
12	11.0
13	11.0
14	19.0
15	48.0
16	94.0
17	230.0
18	635.0
19	1372.0
20	2615.0
21	4419.0
22	7657.0
23	13998.0
24	27216.0
25	53081.0
26	79272.0
27	74834.0
28	57840.0
29	48569.0
30	47227.0
31	51354.0
32	60744.0
33	76556.0
34	104838.0
35	139190.0
36	192278.0
37	281735.0
38	476779.0
39	320952.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.068579393220226	4.17663761318849	44.64351806071565	33.111264932875635
2	35.81747634990371	3.8182485979460083	41.944887859224835	18.419387192925456
3	15.063216036389834	3.8976394637584932	62.17679771339238	18.862346786459288
4	13.75034727617756	4.125123018171373	62.3544618514176	19.77006785423347
5	12.282605112847099	5.116896692989024	62.25209189751703	20.348406296646843
6	15.461818455786444	5.642684597889502	65.07979111632221	13.815705830001837
7	62.05997165284626	1.3451713307623123	30.481006936105892	6.113850080285543
8	60.85145055493556	1.7463636063983576	29.556145728856176	7.846040109809904
9	54.63847961312257	4.68782814655761	33.19470539208069	7.478986848239133
10	24.435387795655632	17.366210381085573	41.23536142621029	16.963040397048506
11	15.874076480809165	18.406296646842492	43.2738137281216	22.44581314422674
12	13.169936948772643	15.151459501711658	49.883079762863346	21.795523786652353
13	17.38113737068377	16.440689937702185	51.27176067844815	14.906412013165887
14	15.304402284724086	18.93726426422184	48.56743279323059	17.190900657823484
15	11.126634552449298	19.343824605517806	50.30277773853753	19.226763103495365
16	10.088055112140776	19.685073481284757	47.862426836561234	22.36444457001323
17	10.215240597644645	20.584977892045377	45.486398545913445	23.71338296439654
18	11.23790419415446	18.43163014969369	49.56382112098396	20.766644535167895
19	11.62360442064916	22.196621885697873	48.46289677774796	17.716876915905015
20	12.070283989508727	24.000433212316413	45.943390451435484	17.98589234673937
21	11.348608776316471	25.994716693271553	45.95116002015379	16.705514510258183
22	9.935394858899924	23.677313330225505	42.88373429016749	23.503557520707076
23	10.377271421642723	24.079353195176274	41.14692960770741	24.39644577547359
24	11.98100458169113	22.24239170869297	41.79335772507028	23.98324598454562
25	10.701851040886767	25.185080544529047	39.14106240611771	24.972006008466472
26	10.695117414664237	26.980698507771923	41.94714809739743	20.37703598016641
27	13.322926820080333	26.01275151035707	41.980533698738505	18.683787970824092
28	10.852486497431334	24.175554582397453	42.41068527596095	22.56127364421026
29	12.508252223744742	22.949187020582293	40.814015360201914	23.72854539547105
30	17.22094299020093	26.1503905974092	36.13094313146581	20.49772328092406
31	13.674393855919234	31.423526254079025	31.970268450371293	22.93181143963045
32	11.750554464676716	30.656599189139556	31.774993289917923	25.817853056265804
33	14.370594301374506	26.035589333559354	30.798570399355835	28.795245965710304
34	13.066248522604736	25.080167822684313	30.31440854746736	31.539175107243594
35	11.961274585976165	27.88159177273305	29.28519967791606	30.871933963374726
36	12.512537258613627	32.73766639826338	27.081420371338293	27.66837597178469
37	11.800091351292808	32.54418059302999	29.7467120597833	25.909015995893903
38	12.580108962315236	26.316894809457214	32.34608013486088	28.756916093366673
39	15.366700099356304	19.357009328191292	31.44287954343189	33.83341102902052
40	15.963026270559928	19.262408943009035	29.46437064138967	35.310194145041365
41	13.750676894244398	22.729802652954557	25.725183291189307	37.79433716161174
42	15.948805605390667	23.969967085281613	28.239462816727645	31.84176449260007
43	15.646781279577334	23.10674445653044	34.70529790409998	26.541176359792246
44	16.045807493631308	22.709366332810653	32.33986447988623	28.904961693671805
45	19.385686100006122	19.210282200153507	31.95256325135261	29.45146844848776
46	20.287756572348812	20.29062895835982	32.65309581997203	26.76851864931934
47	14.261679074432468	22.962136301779466	34.61361699322399	28.162567630564073
48	14.362118408227268	20.508506500539163	36.28106061676249	28.84831447447108
49	19.966708575249452	15.960059707958393	36.44139626213112	27.631835454661037
50	18.35388737421539	13.86269994867376	34.630521691223215	33.15289098588764
51	16.544190010689043	13.789713091016967	31.762750333149686	37.903346565144304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	644.0
1	1478.0
2	2312.0
3	43895.0
4	85478.0
5	58974.5
6	32471.0
7	32091.0
8	31711.0
9	32687.5
10	33664.0
11	34041.0
12	34418.0
13	33463.5
14	32509.0
15	31484.5
16	30460.0
17	28956.5
18	27453.0
19	25903.5
20	24354.0
21	22723.0
22	21092.0
23	19953.0
24	18814.0
25	18023.0
26	16826.0
27	16420.0
28	16685.0
29	16950.0
30	17938.0
31	18926.0
32	20799.5
33	22673.0
34	25524.0
35	28375.0
36	33456.5
37	38538.0
38	44624.0
39	50710.0
40	63678.5
41	76647.0
42	97111.5
43	117576.0
44	141201.0
45	164826.0
46	210605.0
47	256384.0
48	297966.5
49	339549.0
50	310589.5
51	281630.0
52	216180.0
53	150730.0
54	110705.0
55	70680.0
56	51915.5
57	33151.0
58	25691.0
59	18231.0
60	14889.5
61	11548.0
62	9587.0
63	7626.0
64	6051.0
65	4476.0
66	3433.0
67	2390.0
68	1876.5
69	1363.0
70	1057.0
71	751.0
72	603.5
73	456.0
74	331.0
75	161.5
76	117.0
77	97.0
78	77.0
79	55.0
80	33.0
81	19.0
82	5.0
83	7.5
84	10.0
85	6.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2123670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.63195358396391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40902713386431	28.37789008913457
2	10.078476210034564	7.5854549786621055
3	4.41993852360434	4.989927640927575
4	2.543479381758062	3.8286439214435446
5	1.6625612388792936	3.128271368600156
6	1.1157031103003767	2.5191652596184766
7	0.8287775107564739	2.1831961771354544
8	0.6328816925406289	1.905325958226357
9	0.4655584077515887	1.5767885152018741
>10	2.5353034903927285	16.47329459556432
>50	0.16391701984851312	4.315856462562717
>100	0.12631440037804853	9.162863657750979
>500	0.010862389305127357	2.8463429506585314
>1k	0.006062728914489688	4.215732266632552
>5k	3.789205571556055E-4	0.9027702630438937
>10k+	7.57841114311211E-4	5.988475894836894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53468	2.517716971092496	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC	21469	1.0109386109894665	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	16986	0.7998417833279182	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	11825	0.5568190914784312	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	11230	0.5288015557972755	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	11011	0.5184892191347996	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	7137	0.3360691632880815	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	6098	0.28714442451039945	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	5758	0.2711344041211676	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC	4813	0.22663596509815553	No Hit
TCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	4593	0.2162765401404173	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	3707	0.17455631053788961	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	3559	0.1675872428390475	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	3525	0.1659862408001243	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT	3373	0.15882881992023243	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	3260	0.15350784255557595	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	3258	0.15341366596505107	No Hit
GTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	2353	0.11079875875253688	No Hit
TTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	2299	0.10825599080836476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2379465736201954E-4	0.0	0.0	0.8604444193306869	0.0
2	4.2379465736201954E-4	0.0	0.0	2.4317808322385304	0.0
3	4.2379465736201954E-4	0.0	0.0	3.5131164446453544	0.0
4	4.2379465736201954E-4	0.0	0.0	4.993337006220364	0.0
5	4.2379465736201954E-4	0.0	0.0	7.906548569222148	0.0
6	4.2379465736201954E-4	0.0	0.0	10.565577514397246	0.0
7	4.2379465736201954E-4	0.0	0.0	13.093936440219055	0.0
8	4.2379465736201954E-4	0.0	0.0	17.091591443114986	0.0
9	4.2379465736201954E-4	0.0	0.0	19.06675707619357	0.0
10	4.2379465736201954E-4	0.0	0.0	22.737101338720233	0.0
11	4.708829526244661E-4	0.0	0.0	29.779862219648063	0.0
12	4.708829526244661E-4	0.0	0.0	35.3581771179138	0.0
13	4.708829526244661E-4	0.0	0.0	37.40195039718977	0.0
14	4.708829526244661E-4	0.0	0.0	38.12433193481096	0.0
15	5.179712478869127E-4	0.0	0.0	38.941549299090724	0.0
16	5.650595431493594E-4	0.0	0.0	41.014328968248364	0.0
17	5.650595431493594E-4	0.0	0.0	43.83171585039107	0.0
18	5.650595431493594E-4	0.0	0.0	46.57644549294382	0.0
19	5.650595431493594E-4	0.0	0.0	48.18243889116482	0.0
20	5.650595431493594E-4	0.0	0.0	49.55741711282826	0.0
21	6.12147838411806E-4	0.0	0.0	51.07582628186112	0.0
22	6.12147838411806E-4	0.0	0.0	52.318486393837084	0.0
23	6.12147838411806E-4	0.0	0.0	53.26731554337538	0.0
24	0.0010359424957738254	0.0	0.0	53.83783732877519	0.0
25	0.001083030791036272	0.0	0.0	54.26525778487242	0.0
26	0.001083030791036272	0.0	0.0	54.592709790127465	0.0
27	0.0011301190862987188	0.0	0.0	54.89760650195181	0.0
28	0.0011301190862987188	0.0	0.0	55.1781114768302	0.0
29	0.0011301190862987188	0.0	0.0	55.43945151553678	0.0
30	0.0011301190862987188	0.0	0.0	55.70385229343542	0.0
31	0.0011301190862987188	0.0	0.0	55.94056515371974	0.0
32	0.0011301190862987188	0.0	0.0	56.160043697938	0.0
33	0.0011301190862987188	0.0	0.0	56.378486299660494	0.0
34	0.0011301190862987188	0.0	0.0	56.578234848163795	0.0
35	0.0011301190862987188	0.0	0.0	56.785376259023295	0.0
36	0.0011772073815611653	0.0	0.0	56.97467120597833	0.0
37	0.0011772073815611653	0.0	0.0	57.18360197205781	0.0
38	0.0011772073815611653	0.0	0.0	57.39309779768043	0.0
39	0.0011772073815611653	0.0	0.0	57.597366822528926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	35	1.2125747E-7	45.000004	2
AGTTCGG	30	2.1663745E-6	45.000004	2
CTATCCG	20	7.0346385E-4	45.0	1
GGCGTTA	25	3.8919265E-5	45.0	8
ACGGGTA	90	0.0	45.0	5
GCGATAC	20	7.0346385E-4	45.0	9
CGGCTCG	20	7.0346385E-4	45.0	1
CGTTTTT	43130	0.0	44.634827	1
TACCGGG	60	3.6379788E-12	41.250004	3
TCGATTG	55	6.184564E-11	40.909092	1
TTCGTTG	425	0.0	40.764706	1
CCTTACG	50	1.0822987E-9	40.5	1
GTTTTTT	47635	0.0	40.446625	2
TACTGCG	45	1.929584E-8	40.0	1
TGTACGG	90	0.0	40.0	2
TGTACCG	45	1.929584E-8	40.0	1
GTTTGCG	250	0.0	39.6	1
CCGTTTG	195	0.0	39.23077	1
CGTTAGG	115	0.0	39.130432	2
GACGGGA	110	0.0	38.863636	4
>>END_MODULE