##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236816
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.957764678062293	33.0	31.0	34.0	30.0	34.0
2	32.1845863455172	34.0	31.0	34.0	30.0	34.0
3	32.26808154854402	33.0	31.0	34.0	30.0	34.0
4	35.84089335180055	37.0	35.0	37.0	35.0	37.0
5	35.920997736639414	37.0	35.0	37.0	35.0	37.0
6	35.91859080467536	37.0	35.0	37.0	35.0	37.0
7	35.86992010674955	37.0	35.0	37.0	35.0	37.0
8	35.72472721437741	37.0	35.0	37.0	35.0	37.0
9	37.553543679481116	39.0	37.0	39.0	35.0	39.0
10	37.17181693804473	39.0	37.0	39.0	34.0	39.0
11	37.177188196743465	39.0	37.0	39.0	34.0	39.0
12	37.14432724140261	39.0	37.0	39.0	34.0	39.0
13	37.08404415242213	39.0	37.0	39.0	33.0	39.0
14	38.199222180933724	40.0	38.0	41.0	33.0	41.0
15	38.20890902641713	40.0	38.0	41.0	33.0	41.0
16	38.3529702384974	40.0	38.0	41.0	33.0	41.0
17	38.33773055874603	40.0	38.0	41.0	33.0	41.0
18	38.0492027565705	39.0	38.0	40.0	34.0	41.0
19	37.67832832241065	38.0	37.0	40.0	34.0	41.0
20	37.11455307073847	38.0	35.0	40.0	33.0	41.0
21	37.01078896696169	38.0	35.0	40.0	33.0	41.0
22	37.00139348692656	38.0	35.0	40.0	33.0	41.0
23	36.895741841767446	38.0	35.0	40.0	33.0	41.0
24	36.76145615161138	38.0	35.0	40.0	33.0	41.0
25	36.69173957840687	38.0	35.0	40.0	33.0	41.0
26	36.682179413553136	38.0	35.0	40.0	33.0	41.0
27	36.64458060266198	38.0	35.0	40.0	33.0	41.0
28	36.57063289642592	38.0	35.0	40.0	33.0	41.0
29	36.39341936355652	38.0	35.0	40.0	32.0	41.0
30	36.15546246875211	38.0	35.0	40.0	31.0	41.0
31	35.78780994527397	38.0	35.0	40.0	30.0	41.0
32	35.373766975204376	38.0	35.0	40.0	28.0	41.0
33	34.79592595094926	38.0	35.0	40.0	23.0	41.0
34	34.36195611782988	38.0	34.0	40.0	20.0	41.0
35	34.0034837173164	38.0	34.0	40.0	18.0	41.0
36	33.76238514289575	37.0	33.0	40.0	15.0	41.0
37	33.5924810823593	37.0	33.0	40.0	15.0	41.0
38	33.38309489223701	37.0	33.0	40.0	15.0	41.0
39	33.35166542801162	37.0	33.0	40.0	15.0	41.0
40	33.245376157016416	37.0	33.0	40.0	13.0	41.0
41	33.06987703533545	37.0	33.0	40.0	12.0	41.0
42	32.81325163840281	36.0	33.0	40.0	10.0	41.0
43	32.65621832984258	36.0	32.0	40.0	10.0	41.0
44	32.55600128369705	36.0	32.0	40.0	10.0	41.0
45	32.492703195730016	36.0	32.0	40.0	10.0	41.0
46	32.20977890007432	35.0	31.0	40.0	10.0	41.0
47	32.05711185055064	35.0	31.0	40.0	10.0	41.0
48	31.945155732720764	35.0	31.0	40.0	10.0	41.0
49	31.96380734409837	35.0	31.0	40.0	10.0	41.0
50	31.79391595162489	35.0	31.0	39.0	10.0	41.0
51	30.97010759408148	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	7.0
12	4.0
13	15.0
14	13.0
15	13.0
16	29.0
17	52.0
18	100.0
19	200.0
20	377.0
21	551.0
22	850.0
23	1461.0
24	2490.0
25	4310.0
26	6767.0
27	7426.0
28	7067.0
29	6677.0
30	6445.0
31	6979.0
32	7717.0
33	9665.0
34	14081.0
35	19948.0
36	25946.0
37	27044.0
38	42807.0
39	37762.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.419228430511453	5.345500304033511	36.10946895480035	38.125802310654684
2	40.10793189649348	4.836666441456659	37.34460509425039	17.710796567799473
3	17.1398891966759	4.956590770893858	61.12298155530031	16.780538477129923
4	14.104621309370987	5.27582595770556	60.94562867373826	19.67392405918519
5	12.416813053172083	5.85602324167286	61.662218769002095	20.064944936152962
6	15.328778460914801	6.5493885548273765	64.31702249847984	13.804810485777988
7	57.87826836024593	3.0610262820079726	33.8317512330248	5.2289541247213025
8	56.959411526248225	3.5795723261941763	33.17343422741706	6.287581920140531
9	52.08558543341666	5.822241740422944	35.94182825484765	6.150344571312749
10	25.761350584419972	20.54337544760489	39.93142355246267	13.763850415512465
11	18.87203567326532	17.636477265049656	43.9898486588744	19.50163840281062
12	15.938112289710155	15.921221539085199	49.25216201607999	18.888504155124654
13	18.381781636375923	16.72859941895818	50.384686845483415	14.504932099182488
14	15.887862306600905	17.643233565299642	50.579775015201676	15.889129112897777
15	12.428214309843929	16.719731774880074	51.28749746638741	19.564556448888588
16	13.430680359435174	17.839588541314775	48.638605499628405	20.09112559962165
17	13.068796027295454	18.13433213972029	47.002736301601246	21.794135531383017
18	14.67848456185393	16.072393757178567	49.78253158570367	19.466590095263832
19	14.10250996554287	19.512617390716844	49.13857171812715	17.246300925613134
20	14.892574826025267	22.428805486115802	46.732484291601914	15.946135396257011
21	13.868995338152828	23.882676846159043	46.87985609080468	15.368471724883454
22	12.476775217890683	21.40395919194649	45.75831024930748	20.360955340855348
23	12.307445442875482	22.6703432200527	43.76857982568745	21.253631511384366
24	12.437504222687657	22.28523410580366	44.55737787987298	20.719883791635702
25	13.275285453685562	23.204090939801365	41.81558678467671	21.705036821836362
26	13.401543814607123	23.337105600972908	43.08112627525168	20.1802243091683
27	14.025234781433687	21.53148435916492	44.4906594149044	19.952621444496994
28	11.63392676170529	21.352864671305994	44.09879400040538	22.91441456658334
29	14.488463617323156	19.795115194919262	43.336176609688536	22.38024457806905
30	19.27361326937369	19.398182555232754	41.873859874332815	19.45434430106074
31	17.490372272143777	24.56548544017296	36.62970407404905	21.31443821363421
32	16.026788730491184	25.11147895412472	35.69142287683265	23.17030943855145
33	16.708752786973854	22.570265522599826	35.444817917708264	25.276163772718057
34	16.551668806161747	21.79371326261739	36.07568745355044	25.578930477670426
35	18.734798324437538	24.1305486115803	33.41539423011959	23.71925883386258
36	18.775336125937436	25.057850820890483	32.483024795621915	23.683788257550166
37	15.082595770556043	25.899432470779	31.80232754543612	27.215644213228835
38	14.538713600432404	30.062580231065468	32.64390919532464	22.754796973177488
39	16.60656374569286	25.863117356935344	36.17069792581582	21.359620971555977
40	18.684548341328288	22.90047969731775	35.963363961894466	22.451607999459497
41	16.265370583068712	27.504053780149988	31.314184852374837	24.91639078440646
42	17.754290250658737	29.3206540098642	32.118606850888455	20.80644888858861
43	16.477771772177555	25.714901020201335	36.56509695290859	21.242230254712517
44	17.135244240254035	22.59771299236538	35.64032835619215	24.626714411188434
45	20.272701168839944	19.96740085129383	34.741740422944396	25.018157556921828
46	19.767245456388082	21.31823863252483	37.43581514762516	21.478700763461926
47	14.528156881291803	26.58984190257415	38.40576650226336	20.476234713870685
48	14.602053915275995	25.960661441794475	39.23341328288629	20.20387136004324
49	19.098371731639755	21.527683940274304	39.46735017904196	19.906594149043983
50	17.69559489223701	19.44252077562327	37.93831497871765	24.923569353422064
51	15.974427403553815	19.181558678467674	35.66608675089521	29.177927167083308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	671.0
1	1144.0
2	1617.0
3	13222.0
4	24827.0
5	14950.0
6	5073.0
7	4849.0
8	4625.0
9	4482.0
10	4339.0
11	4190.0
12	4041.0
13	3846.0
14	3651.0
15	3365.5
16	3080.0
17	2765.0
18	2450.0
19	2231.5
20	2013.0
21	1878.0
22	1743.0
23	1667.5
24	1592.0
25	1569.0
26	1605.5
27	1665.0
28	1734.5
29	1804.0
30	1929.5
31	2055.0
32	2181.0
33	2307.0
34	2650.0
35	2993.0
36	3095.5
37	3198.0
38	3591.5
39	3985.0
40	5173.0
41	6361.0
42	8446.0
43	10531.0
44	11479.5
45	12428.0
46	15535.0
47	18642.0
48	22507.5
49	26373.0
50	26355.0
51	26337.0
52	21811.0
53	17285.0
54	13886.0
55	10487.0
56	8904.0
57	7321.0
58	6385.5
59	5450.0
60	4884.0
61	4318.0
62	3773.0
63	3228.0
64	2905.5
65	2583.0
66	2236.0
67	1889.0
68	1626.5
69	1364.0
70	1171.5
71	979.0
72	862.0
73	745.0
74	660.5
75	445.5
76	315.0
77	262.5
78	210.0
79	144.0
80	78.0
81	53.0
82	28.0
83	16.5
84	5.0
85	5.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	236816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.97067463277552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29816176717814	34.68191594681074
2	14.148327356011265	13.574096163864272
3	5.869830444410624	8.447391791951466
4	2.8921571346640054	5.549549155753094
5	1.4374895209567984	3.4478671048921465
6	0.8344672830947746	2.4017975117421364
7	0.5274945538419914	1.7712988729040684
8	0.36022086991821317	1.382403051742557
9	0.24542183990070848	1.0595746106688613
>10	1.2785628822911983	11.170580534041484
>50	0.07125116547451102	2.342256471318188
>100	0.030677585134858913	2.8141257320580095
>500	9.895995204793199E-4	0.27628562349152525
>1k	0.0039583980819172795	2.7932381591479976
>5k	0.0	0.0
>10k+	9.895995204793199E-4	8.287619269613483
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17458	7.371968110262821	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC	2552	1.077629889872306	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	1301	0.5493716640767515	No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1022	0.43155867846767115	No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT	1009	0.42606918451455983	No Hit
TCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	582	0.2457604215931356	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	498	0.21028984528072428	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	451	0.1904432132963989	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	394	0.16637389365583408	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.1629957435308425	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	264	0.1114789541247213	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTG	262	0.11063441659347341	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.10176677251537058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4868758867644078	0.0
2	0.0	0.0	0.0	1.5636612391054658	0.0
3	0.0	0.0	0.0	2.1404803729477737	0.0
4	0.0	0.0	0.0	2.9288561583676778	0.0
5	0.0	0.0	0.0	4.585416525910412	0.0
6	4.2226876562394435E-4	0.0	0.0	6.188348760218904	0.0
7	4.2226876562394435E-4	0.0	0.0	7.78199108168367	0.0
8	4.2226876562394435E-4	0.0	0.0	10.197368421052632	0.0
9	4.2226876562394435E-4	0.0	0.0	11.330737787987298	0.0
10	4.2226876562394435E-4	0.0	0.0	13.615211810012838	0.0
11	4.2226876562394435E-4	0.0	0.0	18.948466319843252	0.0
12	4.2226876562394435E-4	0.0	0.0	23.370042564691573	0.0
13	4.2226876562394435E-4	0.0	0.0	24.98099790554692	0.0
14	4.2226876562394435E-4	0.0	0.0	25.581041821498548	0.0
15	4.2226876562394435E-4	0.0	0.0	26.34112559962165	0.0
16	4.2226876562394435E-4	0.0	0.0	27.969393959867578	0.0
17	4.2226876562394435E-4	0.0	0.0	29.993328153503143	0.0
18	4.2226876562394435E-4	0.0	0.0	31.894382136342138	0.0
19	4.2226876562394435E-4	0.0	0.0	33.04295317883927	0.0
20	4.2226876562394435E-4	0.0	0.0	34.0804675359773	0.0
21	4.2226876562394435E-4	0.0	0.0	35.283511249239915	0.0
22	4.2226876562394435E-4	0.0	0.0	36.277954192284305	0.0
23	4.2226876562394435E-4	0.0	0.0	37.06886359029795	0.0
24	4.2226876562394435E-4	0.0	0.0	37.51984663198432	0.0
25	4.2226876562394435E-4	0.0	0.0	37.81754611174921	0.0
26	4.2226876562394435E-4	0.0	0.0	38.059083845686104	0.0
27	4.2226876562394435E-4	0.0	0.0	38.27190730356057	0.0
28	4.2226876562394435E-4	0.0	0.0	38.46784001081008	0.0
29	8.445375312478887E-4	0.0	0.0	38.68150800621579	0.0
30	8.445375312478887E-4	0.0	0.0	38.91037767718397	0.0
31	8.445375312478887E-4	0.0	0.0	39.07464022701169	0.0
32	8.445375312478887E-4	0.0	0.0	39.29675359772988	0.0
33	8.445375312478887E-4	0.0	0.0	39.46650564151071	0.0
34	8.445375312478887E-4	0.0	0.0	39.64132491047902	0.0
35	8.445375312478887E-4	0.0	0.0	39.8119214917911	0.0
36	0.001266806296871833	0.0	0.0	39.95675967840011	0.0
37	0.001266806296871833	0.0	0.0	40.11426592797784	0.0
38	0.001266806296871833	0.0	0.0	40.327089385852304	0.0
39	0.001266806296871833	0.0	0.0	40.6408350787109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	25	3.881398E-5	45.0	1
ATGGGAT	20	7.0219336E-4	45.0	5
ATGGGAC	25	3.881398E-5	45.0	5
GGTACAA	20	7.0219336E-4	45.0	8
CTCGTGG	40	6.7757355E-9	45.0	2
ACGTTGG	20	7.0219336E-4	45.0	2
GGGTAAC	25	3.881398E-5	45.0	7
TATGCGG	20	7.0219336E-4	45.0	2
TGTTGCG	20	7.0219336E-4	45.0	1
GCCGATG	25	3.881398E-5	45.0	9
AGTGCGC	25	3.881398E-5	45.0	13
TGTTCCG	25	3.881398E-5	45.0	1
AGATTAT	20	7.0219336E-4	45.0	6
CTACCAT	20	7.0219336E-4	45.0	3
CGCACCC	20	7.0219336E-4	45.0	31
GCTACCA	20	7.0219336E-4	45.0	2
CACGTTG	20	7.0219336E-4	45.0	1
AGGGCGA	20	7.0219336E-4	45.0	6
TACGTTG	20	7.0219336E-4	45.0	24
AAGCTCC	20	7.0219336E-4	45.0	34
>>END_MODULE