FastQCFastQC Report
Sat 14 Jan 2017
SRR2933687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900790
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515785.725862853717293No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC14520.16119184271583833No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11300.1254454423339513No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10210.11334495276368521No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC253.890131E-545.033
CCGCTTG207.0324726E-445.01
GCTAGCG253.890131E-545.01
TCATCCG253.890131E-545.01
CGTTTTT307000.044.6848531
GTTTTTT345600.040.201822
CTCGTTG850.039.7058831
CGCCGGT1050.038.5714328
CTTCGCG950.037.8947371
TTGTACG301.1397258E-437.5000041
CATCCGG301.1397258E-437.5000042
TTTCGCG1950.036.9230771
CACGCCG1100.036.8181826
TATTGCG552.748493E-936.818181
GCCGATT1650.036.818189
CGGTAGT1100.036.8181812
CTTTGCG1900.036.710531
TGGGCGA13550.036.5313646
GTTTGCG1750.036.01
CGGGTCT250.00210686736.06
Produced by FastQC (version 0.11.5)